FastQCFastQC Report
Fri 27 May 2016
SRR2138656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7660427
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144850.049.6595651
GTATCAA257700.036.7643131
ATCAACG316350.029.4757733
TCAACGC320700.029.1684364
CAACGCA322500.028.9223885
TATCAAC326450.028.851692
AACGCAG327750.028.5726456
ATCTCGT113600.027.10817594
ACGCAGA366250.025.4758327
CGCAGAG370250.025.1775368
GCAGAGT409550.022.6989659
GTGGTAT70250.021.4030481
GAGTACT243950.021.03185312-13
TATCTCG136700.020.52703594
TACATGG290350.020.346032
GTACATG303900.019.9589161
CAGAGTA412700.019.7837210-11
TGGTATC72100.019.6689912
ACATGGG299300.019.5235443
GTACTTT268050.019.10973214-15
Produced by FastQC (version 0.11.5)