##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7660427
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79812221955773	33.0	33.0	33.0	33.0	33.0
2	32.79260085632302	33.0	33.0	33.0	33.0	33.0
3	32.790730595043854	33.0	33.0	33.0	33.0	33.0
4	36.73764726169964	37.0	37.0	37.0	37.0	37.0
5	36.75256810096879	37.0	37.0	37.0	37.0	37.0
6	36.762310769360504	37.0	37.0	37.0	37.0	37.0
7	36.75136372946312	37.0	37.0	37.0	37.0	37.0
8	36.74493027607991	37.0	37.0	37.0	37.0	37.0
9	36.74042517473243	37.0	37.0	37.0	37.0	37.0
10-11	36.714535821567125	37.0	37.0	37.0	37.0	37.0
12-13	36.69345364168342	37.0	37.0	37.0	37.0	37.0
14-15	38.95859192966658	40.0	40.0	40.0	37.0	40.0
16-17	38.9130652899636	40.0	40.0	40.0	37.0	40.0
18-19	38.951924873117385	40.0	40.0	40.0	37.0	40.0
20-21	38.98801874877417	40.0	40.0	40.0	37.0	40.0
22-23	39.01163479628855	40.0	40.0	40.0	37.0	40.0
24-25	39.01582816790195	40.0	40.0	40.0	37.0	40.0
26-27	38.92500717768101	40.0	40.0	40.0	37.0	40.0
28-29	38.85224417734665	40.0	40.0	40.0	37.0	40.0
30-31	38.78123731034647	40.0	37.0	40.0	37.0	40.0
32-33	38.461017056116916	40.0	37.0	40.0	37.0	40.0
34-35	38.604651908690684	40.0	37.0	40.0	37.0	40.0
36-37	38.57553294245268	40.0	37.0	40.0	37.0	40.0
38-39	38.603119901905984	40.0	37.0	40.0	37.0	40.0
40-41	38.574450452265495	40.0	37.0	40.0	37.0	40.0
42-43	38.27518351718581	40.0	37.0	40.0	37.0	40.0
44-45	38.176022376251254	40.0	37.0	40.0	37.0	40.0
46-47	38.196527005024635	40.0	37.0	40.0	37.0	40.0
48-49	38.060280401015675	40.0	37.0	40.0	37.0	40.0
50-51	38.1560072489495	40.0	37.0	40.0	37.0	40.0
52-53	38.1409088472375	40.0	37.0	40.0	37.0	40.0
54-55	38.083117425214176	40.0	37.0	40.0	37.0	40.0
56-57	37.96542721468163	40.0	37.0	40.0	35.0	40.0
58-59	37.8364290857908	40.0	37.0	40.0	33.0	40.0
60-61	37.59280603675721	38.5	37.0	40.0	33.0	40.0
62-63	37.408172031220474	37.0	37.0	40.0	33.0	40.0
64-65	37.24554891261709	37.0	37.0	40.0	33.0	40.0
66-67	37.01627081124444	37.0	37.0	40.0	33.0	40.0
68-69	36.74794553380228	37.0	37.0	40.0	33.0	40.0
70-71	36.30901878366164	37.0	37.0	38.5	33.0	40.0
72-73	36.21396706449154	37.0	37.0	37.0	33.0	40.0
74-75	35.98130953382474	37.0	37.0	37.0	33.0	40.0
76-77	33.90452620926705	35.0	33.0	37.0	27.0	37.0
78-79	35.32601940707844	37.0	33.0	37.0	33.0	37.0
80-81	35.63454547454095	37.0	37.0	37.0	33.0	37.0
82-83	35.639488620755834	37.0	37.0	37.0	33.0	37.0
84-85	35.55676716914219	37.0	37.0	37.0	33.0	37.0
86-87	35.13585642549147	37.0	33.0	37.0	33.0	37.0
88-89	35.06539407025926	37.0	33.0	37.0	33.0	37.0
90-91	35.2527874602574	37.0	33.0	37.0	33.0	37.0
92-93	35.26226556952177	37.0	33.0	37.0	33.0	37.0
94-95	35.21240782932663	37.0	33.0	37.0	33.0	37.0
96-97	35.16687052540067	37.0	33.0	37.0	33.0	37.0
98-99	35.13750158926224	37.0	33.0	37.0	33.0	37.0
100	35.0776132147015	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	7.0
20	27.0
21	113.0
22	492.0
23	1446.0
24	3632.0
25	7869.0
26	14248.0
27	23878.0
28	36539.0
29	52323.0
30	71135.0
31	95462.0
32	128104.0
33	175093.0
34	250712.0
35	393746.0
36	800683.0
37	2348290.0
38	3133958.0
39	122668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.105355223670955	20.14425566616587	14.46256194334859	23.287827166814594
2	14.842057759965599	20.502107780675935	41.74759448787907	22.908239971479397
3	18.06279397429951	26.338883187793304	30.481725243628826	25.11659759427836
4	12.303558289000726	17.899415159479883	38.862336615173476	30.934689936345915
5	13.21422868922007	37.875464828888525	35.30935739082992	13.600949091061484
6	29.82145773336134	37.84358234860798	18.476567951107686	13.858391966922992
7	26.4786414647643	32.96170043784766	22.673892721645935	17.88576537574211
8	25.03447914848611	35.68315447689796	20.79824009810419	18.484126276511738
9	25.94098214107386	16.60129389654128	21.07336836445279	36.38435559793207
10-11	24.48901347144226	26.695430946603892	28.294538933665187	20.521016648288665
12-13	25.502801606229	24.30787474379692	28.358353914213918	21.83096973576016
14-15	22.661602284050222	25.480139684119436	26.625774255142698	25.23248377668764
16-17	21.649054289010262	28.59601037905589	27.248761720462838	22.506173611471006
18-19	21.562107438658444	27.578913029260637	29.42780474247715	21.431174789603766
20-21	22.793164437010407	26.69455213767833	29.2678911809995	21.244392244311765
22-23	22.396046469015793	26.607225140931067	29.130814722199066	21.865913667854077
24-25	21.991336937577856	26.761949776019335	29.418547384075993	21.828165902326816
26-27	21.93863339890055	26.9953260082307	29.21527444959639	21.850766143272367
28-29	21.750810140626665	27.146808082225654	29.323098266283036	21.779283510864644
30-31	22.106716692333308	26.83267521289997	29.275467197834637	21.785140896932088
32-33	21.971197327108175	27.086839509312497	28.989291115303846	21.952672048275485
34-35	21.967260040370867	27.141006450025362	29.16297469803028	21.72875881157349
36-37	22.110300004335564	27.031705533931067	29.092738992885376	21.765255468847993
38-39	21.865388820881396	27.132968839198828	29.098346200037504	21.903296139882265
40-41	22.21167421727773	26.929775399595535	29.088220437350376	21.770329945776364
42-43	22.17534863715335	27.073770696585576	28.697073088716195	22.053807577544884
44-45	22.178325110723083	27.322572846734843	28.398771535549876	22.1003305069922
46-47	22.262618006942052	27.162493402620964	28.141316462622463	22.43357212781452
48-49	22.374757754911005	27.346981154810372	27.929408358557318	22.348852731721305
50-51	22.083182615197046	27.580781929562388	27.67898011843894	22.657055336801626
52-53	22.05806010214748	27.535805736095735	27.613392720133817	22.792741441622965
54-55	22.400802286641508	27.238064673176705	27.74661462770195	22.61451841247984
56-57	22.338986344683093	27.04653503337407	27.689100215826777	22.925378406116064
58-59	21.938543556586755	26.93583902164559	28.08540317967458	23.040214242093082
60-61	22.311252387165595	27.316748908660376	27.952784683480186	22.41921402069384
62-63	21.93310430500423	27.388972996970374	27.91569624438629	22.762226453639105
64-65	21.607623940050598	27.759847580721814	27.643500884490198	22.989027594737394
66-67	21.773704168301034	28.276445078303347	27.131040854595334	22.81880989880029
68-69	21.940019943665437	28.11446291000162	27.051900213769347	22.893616932563592
70-71	21.95388102452553	27.471032469399397	27.036319277902614	23.538767228172457
72-73	22.23552510143709	27.07694123110769	27.10968418389329	23.57784948356193
74-75	22.19396467561356	27.177350909435326	27.233452028828214	23.395232386122892
76-77	22.54807015263289	27.080011946932096	26.562463729764108	23.8094541706709
78-79	22.812735236751447	27.042607955479873	26.180409473573597	23.96424733419508
80-81	22.43902404370308	27.367436949650337	26.500888326596026	23.692650680050555
82-83	22.651261350044777	27.08419539065593	26.56781441390683	23.69672884539246
84-85	22.909026753973507	27.398256915131807	26.348949999951643	23.343766330943044
86-87	22.992907340483573	27.412358983843994	26.578826725058313	23.01590695061412
88-89	23.094876588806656	27.360769676393694	26.82109932933835	22.7232544054613
90-91	22.972594055644922	26.982597112875105	26.788208921280905	23.256599910199068
92-93	22.933302309006347	26.58397018694913	26.96285112440125	23.51987637964327
94-95	22.150825696797384	26.958247484479653	27.625598148520925	23.26532867020204
96-97	22.228888682175455	26.89286298852691	27.85908973554961	23.019158593748028
98-99	23.43022581416434	28.796331673875013	28.127387690654242	19.646054821306404
100	22.664445701744544	29.80324447086366	21.67219321678639	25.860116610605406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1476.0
1	1472.0
2	2419.5
3	3634.5
4	5502.5
5	9571.5
6	12559.5
7	14253.0
8	15210.5
9	14656.0
10	13241.0
11	12017.5
12	13527.5
13	18872.5
14	30391.0
15	45350.0
16	57066.0
17	63643.5
18	64354.0
19	59444.5
20	51641.5
21	43387.5
22	37555.5
23	36689.5
24	40240.0
25	47359.5
26	58733.0
27	72689.0
28	84522.0
29	97058.5
30	109310.5
31	120866.5
32	138421.5
33	157433.0
34	175085.0
35	193221.5
36	214681.5
37	236051.0
38	245399.5
39	249362.0
40	258658.5
41	264474.5
42	268180.5
43	278519.0
44	288955.5
45	298504.5
46	307422.0
47	316959.0
48	327205.5
49	328170.0
50	322394.0
51	315417.5
52	304894.5
53	291779.5
54	276062.5
55	259124.0
56	240025.0
57	216947.0
58	191863.5
59	167334.0
60	139843.5
61	110118.5
62	85063.5
63	63983.0
64	46966.0
65	34049.5
66	24568.0
67	18144.0
68	13546.0
69	9905.5
70	7127.0
71	5071.5
72	3627.5
73	2414.5
74	1536.0
75	981.0
76	616.5
77	377.0
78	245.0
79	161.0
80	110.5
81	77.5
82	57.0
83	40.5
84	29.0
85	16.5
86	10.5
87	9.0
88	6.5
89	3.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001383734875353554
4	0.01178785464570056
5	0.011592043106735435
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.263595092806201E-5
22-23	0.0
24-25	0.005314479770210774
26-27	0.04570953422905473
28-29	0.0815148710568692
30-31	0.07466835243069701
32-33	0.033874945278051155
34-35	0.002713535662543651
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.415664712270748E-4
56-57	3.0497939864162176E-4
58-59	7.106910390103997E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.6545004480312007E-4
72-73	0.012785733429605957
74-75	0.02821125470417499
76-77	0.0026616808638454293
78-79	0.0
80-81	0.0
82-83	0.006802944071744662
84-85	0.04544403966496923
86-87	0.03815794572665201
88-89	0.008921823917105392
90-91	4.9981939859064254E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	443.0
22-23	992.0
24-25	2272.0
26-27	2851.0
28-29	8654.0
30-31	20067.0
32-33	25558.0
34-35	35166.0
36-37	47340.0
38-39	35852.0
40-41	60274.0
42-43	68293.0
44-45	83417.0
46-47	80664.0
48-49	69394.0
50-51	38503.0
52-53	13411.0
54-55	13658.0
56-57	14569.0
58-59	38112.0
60-61	65188.0
62-63	64729.0
64-65	67192.0
66-67	71661.0
68-69	67078.0
70-71	64389.0
72-73	61443.0
74-75	61933.0
76-77	61315.0
78-79	64871.0
80-81	65900.0
82-83	62879.0
84-85	64425.0
86-87	65627.0
88-89	70869.0
90-91	75594.0
92-93	78726.0
94-95	85680.0
96-97	256798.0
98-99	497900.0
100-101	5026644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.22881390072637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31723718540798	69.18041135288271
2	8.038846132729569	12.738164884532097
3	2.397296803694027	5.698049469740407
4	0.9566743413074091	3.031846934041788
5	0.4676971573465775	1.8527545520655362
6	0.2542113478393866	1.2084518141651746
7	0.1559297977614968	0.8647893049896469
8	0.09659768992928253	0.6122656318917755
9	0.06579833557487277	0.4691811675815224
>10	0.23827253359899164	2.950097629465029
>50	0.006932880964896816	0.36795248834687155
>100	0.003978066284281411	0.5831702392050746
>500	3.8700021388035025E-4	0.24420544963287555
>1k	1.4072734728431084E-4	0.19865908145957142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3054102597675038E-5	0.0
5	0.0	0.0	0.0	1.3054102597675038E-5	0.0
6	0.0	0.0	0.0	1.3054102597675038E-5	1.3054102597675038E-5
7	0.0	0.0	0.0	1.3054102597675038E-5	1.3054102597675038E-5
8	0.0	0.0	0.0	1.3054102597675038E-5	1.3054102597675038E-5
9	0.0	0.0	0.0	3.916230779302511E-5	1.3054102597675038E-5
10-11	0.0	0.0	0.0	5.2216410390700145E-5	1.3054102597675038E-5
12-13	0.0	0.0	0.0	7.832461558605022E-5	1.3054102597675038E-5
14-15	0.0	0.0	0.0	1.3054102597675037E-4	5.8743461689537665E-5
16-17	0.0	0.0	0.0	1.8928448766628806E-4	1.044328207814003E-4
18-19	0.0	0.0	0.0	2.676091032523383E-4	1.3054102597675037E-4
20-21	0.0	0.0	0.0	4.373124370221138E-4	1.3054102597675037E-4
22-23	0.0	0.0	0.0	0.0010247470539174906	1.5012217987326291E-4
24-25	0.0	0.0	0.0	0.003237417444223409	1.697033337697755E-4
26-27	0.0	0.0	0.0	0.00820450348263876	1.8275743636745053E-4
28-29	0.0	0.0	0.0	0.024528658781031398	1.9581153896512557E-4
30-31	0.0	0.0	0.0	0.028986634818137423	1.9581153896512557E-4
32-33	0.0	0.0	0.0	0.03662981188907616	1.9581153896512557E-4
34-35	0.0	0.0	0.0	0.044873477679507945	2.3497384675815069E-4
36-37	0.0	0.0	0.0	0.0611323624649122	2.3497384675815069E-4
38-39	0.0	0.0	0.0	0.22231789428970472	2.5455500065466323E-4
40-41	0.0	0.0	0.0	0.39141421228868833	2.6108205195350074E-4
42-43	0.0	0.0	0.0	0.42734562968878886	2.6108205195350074E-4
44-45	0.0	0.0	0.0	0.4789941866164902	2.6760910325233824E-4
46-47	0.0	0.0	0.0	0.5302054311071693	3.067714110453634E-4
48-49	0.0	0.0	0.0	0.577337268536075	3.39406667539551E-4
50-51	0.0	0.0	0.0	0.6215776222395958	3.39406667539551E-4
52-53	0.0	0.0	0.0	1.0940382305059495	3.52460770137226E-4
54-55	0.0	0.0	0.0	1.723428733150254	3.6551487273490105E-4
56-57	0.0	0.0	0.0	2.407652993756092	3.7204192403373856E-4
58-59	0.0	0.0	0.0	3.110107308639584	3.785689753325761E-4
60-61	0.0	0.0	0.0	3.303869353496874	3.785689753325761E-4
62-63	0.0	0.0	0.0	3.3109968935152048	3.9162307793025113E-4
64-65	0.0	0.0	0.0	3.3173216062237785	3.9162307793025113E-4
66-67	0.0	0.0	0.0	3.3225758825193425	3.9162307793025113E-4
68-69	0.0	0.0	6.527051298837519E-6	3.3281695654824466	3.981501292290887E-4
70-71	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3331301244695632	4.112042318267637E-4
72-73	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3368897060176934	4.8300179611397644E-4
74-75	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3398921496151583	4.8300179611397644E-4
76-77	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3429011202639227	4.8300179611397644E-4
78-79	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.345055047192539	4.8300179611397644E-4
80-81	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3456751170659285	4.8300179611397644E-4
82-83	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3461189565542497	4.8300179611397644E-4
84-85	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3462886598880193	4.8300179611397644E-4
86-87	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.346380038606203	4.960558987116515E-4
88	0.0	1.3054102597675038E-5	1.3054102597675038E-5	3.3463930927088006	4.960558987116515E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14485	0.0	49.659565	1
GTATCAA	25770	0.0	36.764313	1
ATCAACG	31635	0.0	29.475773	3
TCAACGC	32070	0.0	29.168436	4
CAACGCA	32250	0.0	28.922388	5
TATCAAC	32645	0.0	28.85169	2
AACGCAG	32775	0.0	28.572645	6
ATCTCGT	11360	0.0	27.108175	94
ACGCAGA	36625	0.0	25.475832	7
CGCAGAG	37025	0.0	25.177536	8
GCAGAGT	40955	0.0	22.698965	9
GTGGTAT	7025	0.0	21.403048	1
GAGTACT	24395	0.0	21.031853	12-13
TATCTCG	13670	0.0	20.527035	94
TACATGG	29035	0.0	20.34603	2
GTACATG	30390	0.0	19.958916	1
CAGAGTA	41270	0.0	19.78372	10-11
TGGTATC	7210	0.0	19.668991	2
ACATGGG	29930	0.0	19.523544	3
GTACTTT	26805	0.0	19.109732	14-15
>>END_MODULE