FastQCFastQC Report
Fri 27 May 2016
SRR2138655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8278609
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132100.050.430131
GTATCAA242500.038.445831
ATCAACG295800.030.9233463
TCAACGC297050.030.836014
TATCAAC303300.030.32852
CAACGCA303200.030.2078045
AACGCAG307300.029.8702756
ACGCAGA352200.026.0866497
CGCAGAG355950.025.7756548
GTGGTAT61750.024.120911
GCAGAGT404250.022.664129
TGGTATC63400.022.613012
GTACATG281800.021.5991381
TACATGG275100.021.5166592
GAGTACT248600.020.68844812-13
ACATGGG280750.020.3344783
CAGAGTA396000.019.67397110-11
AGAGTAC371850.019.12831710-11
GTACTTT277800.018.45273214-15
CATGGGG204250.017.9889164
Produced by FastQC (version 0.11.5)