##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8278609
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79052362540615	33.0	33.0	33.0	33.0	33.0
2	32.78288369459169	33.0	33.0	33.0	33.0	33.0
3	32.78004070490586	33.0	33.0	33.0	33.0	33.0
4	36.724898228675855	37.0	37.0	37.0	37.0	37.0
5	36.74028777056629	37.0	37.0	37.0	37.0	37.0
6	36.748937049690355	37.0	37.0	37.0	37.0	37.0
7	36.73644171381932	37.0	37.0	37.0	37.0	37.0
8	36.72809139796311	37.0	37.0	37.0	37.0	37.0
9	36.72470785853034	37.0	37.0	37.0	37.0	37.0
10-11	36.6964467098277	37.0	37.0	37.0	37.0	37.0
12-13	36.67588419745395	37.0	37.0	37.0	37.0	37.0
14-15	38.92057753905276	40.0	40.0	40.0	37.0	40.0
16-17	38.87741056498743	40.0	38.5	40.0	37.0	40.0
18-19	38.908438603634984	40.0	40.0	40.0	37.0	40.0
20-21	38.94274524474113	40.0	40.0	40.0	37.0	40.0
22-23	38.96580354940677	40.0	40.0	40.0	37.0	40.0
24-25	38.97242781982685	40.0	40.0	40.0	37.0	40.0
26-27	38.8810855217463	40.0	40.0	40.0	37.0	40.0
28-29	38.80868296393197	40.0	38.5	40.0	37.0	40.0
30-31	38.74195040003826	40.0	37.0	40.0	37.0	40.0
32-33	38.41248075127838	40.0	37.0	40.0	37.0	40.0
34-35	38.55430564167044	40.0	37.0	40.0	37.0	40.0
36-37	38.52553532432855	40.0	37.0	40.0	37.0	40.0
38-39	38.56259476757805	40.0	37.0	40.0	37.0	40.0
40-41	38.52577961506226	40.0	37.0	40.0	37.0	40.0
42-43	38.21218280640998	40.0	37.0	40.0	37.0	40.0
44-45	38.11815701094719	40.0	37.0	40.0	35.0	40.0
46-47	38.148118917132265	40.0	37.0	40.0	37.0	40.0
48-49	38.00815519918535	40.0	37.0	40.0	35.0	40.0
50-51	38.118921685697885	40.0	37.0	40.0	37.0	40.0
52-53	38.104129733109986	40.0	37.0	40.0	37.0	40.0
54-55	38.042838247264314	40.0	37.0	40.0	37.0	40.0
56-57	37.934664882888335	40.0	37.0	40.0	33.0	40.0
58-59	37.80554717685626	40.0	37.0	40.0	33.0	40.0
60-61	37.56218510393634	40.0	37.0	40.0	33.0	40.0
62-63	37.372718398215376	37.0	37.0	40.0	33.0	40.0
64-65	37.20600330656668	37.0	37.0	40.0	33.0	40.0
66-67	36.97454787282549	37.0	37.0	40.0	33.0	40.0
68-69	36.70879714454131	37.0	37.0	40.0	33.0	40.0
70-71	36.2537723153074	37.0	37.0	40.0	33.0	40.0
72-73	36.16186911116965	37.0	37.0	37.0	33.0	40.0
74-75	35.92778416950602	37.0	37.0	37.0	33.0	40.0
76-77	33.87996376201662	35.0	33.0	37.0	27.0	37.0
78-79	35.28601086847195	37.0	33.0	37.0	33.0	37.0
80-81	35.5973981073234	37.0	37.0	37.0	33.0	37.0
82-83	35.59947760431264	37.0	37.0	37.0	33.0	37.0
84-85	35.49757482397776	37.0	35.0	37.0	33.0	37.0
86-87	35.03678978520949	37.0	33.0	37.0	33.0	37.0
88-89	34.96628985088667	37.0	33.0	37.0	33.0	37.0
90-91	35.14825690086812	37.0	33.0	37.0	33.0	37.0
92-93	35.14325193393076	37.0	33.0	37.0	33.0	37.0
94-95	35.11824738368101	37.0	33.0	37.0	33.0	37.0
96-97	35.07902629309996	37.0	33.0	37.0	33.0	37.0
98-99	35.04426770376175	37.0	33.0	37.0	33.0	37.0
100	34.989100855404395	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	33.0
21	128.0
22	529.0
23	1727.0
24	4157.0
25	8861.0
26	15981.0
27	26491.0
28	40452.0
29	58344.0
30	80423.0
31	109054.0
32	147385.0
33	201078.0
34	289379.0
35	449683.0
36	893730.0
37	2480164.0
38	3342727.0
39	128277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.085753778201145	19.974611676913355	14.55304870661243	23.38658583827307
2	14.763784592314966	20.603328409398245	41.772367797537	22.86051920074979
3	18.365198278487263	25.878220211237906	30.2056656656439	25.55091584463093
4	12.542701717974769	17.919149722691014	38.7054363675241	30.832712191810113
5	13.451256595627523	37.61709704078806	35.234558919731136	13.697087443853281
6	30.05207758936314	37.52547076447263	18.670866083903707	13.751585562260521
7	26.726325642387504	32.83947822635421	22.618449548710416	17.815746582547863
8	24.692578185538174	36.30949353931319	20.790727041221537	18.2072012339271
9	25.790141797975963	17.019392992228525	21.20602627808609	35.98443893170942
10-11	24.4346544208091	26.77966189730666	28.39097727649657	20.39470640538767
12-13	25.344004047056696	24.41582275476472	28.633318713324908	21.606854484853674
14-15	22.40175251663655	25.714241365910627	26.72526266187955	25.158743455573273
16-17	21.627739635970244	28.814321343114525	27.356316743549552	22.20162227736568
18-19	21.549749480860854	27.859722569335016	29.433332338802327	21.1571956110018
20-21	22.55761106522866	26.89957275565656	29.423683390065303	21.11913278904948
22-23	22.274931900139087	26.82743410379182	29.223059669654745	21.67457432641435
24-25	21.96296179712202	26.986151078365168	29.353286613812717	21.697600510700095
26-27	21.909580229942545	27.151651362235086	29.21470236349051	21.724066044331863
28-29	21.824121718410595	27.27380881493649	29.27804185891822	21.624027607734696
30-31	22.09746821127116	27.09257032186139	29.16703290033257	21.64292856653488
32-33	21.945854313495442	27.148428872456957	29.02021206933125	21.88550474471635
34-35	22.052372809347755	27.295517075584364	29.050915795808873	21.601194319259008
36-37	22.06213481431705	27.196877757237957	28.965796160731127	21.77519126771387
38-39	21.928378114809526	27.334799147711546	29.053694197145035	21.683128540333893
40-41	22.192653065760144	27.264634895823203	28.827223543872353	21.715488494544296
42-43	22.116237785682262	27.385948639181546	28.55125473804103	21.94655883709516
44-45	22.268335100391525	27.493846621604646	28.321535298843344	21.916282979160492
46-47	22.209076421036656	27.532670562343476	28.045325187827274	22.21292782879259
48-49	22.268505156787015	27.65686565847023	27.906918573054	22.167710611688758
50-51	22.216652349342368	27.776125751337982	27.759894162840386	22.24732773647926
52-53	22.315629497623103	27.738937562698062	27.608835034370323	22.33659790530851
54-55	22.351379391926386	27.647262076288758	27.68745116045655	22.313907371328305
56-57	22.335335269596133	27.744270023042134	27.645038140143235	22.275356567218495
58-59	22.266338455634187	27.845870943233603	27.676243555880596	22.211547045251613
60-61	22.462835390616743	27.727916005227442	27.6056490259506	22.20359957820521
62-63	22.407488059672378	27.790507974977146	27.51932717881945	22.282676786531027
64-65	22.318675052860563	27.773505500949426	27.546091458750254	22.361727987439753
66-67	22.395400927373363	27.81640169442097	27.39157113515695	22.39662624304872
68-69	22.31888572476256	27.80693646110035	27.458890192033625	22.415287622103463
70-71	22.311265267060367	27.57655614218629	27.466883639426833	22.64529495132651
72-73	22.41341527973085	27.64816180143383	27.423222724033796	22.515200194801526
74-75	22.29455203308282	27.756618101437724	27.417773898184418	22.53105596729504
76-77	22.456512744409256	27.70606310013328	27.2225611263959	22.614863029061564
78-79	22.42351078099848	27.70590127415541	27.21535269427462	22.655235250571494
80-81	22.387398458395776	27.75747531333601	27.29712112385946	22.558005104408753
82-83	22.416077717595766	27.739473914506828	27.396768475413296	22.44767989248411
84-85	22.633771639051297	27.732446499838247	27.241890616171755	22.3918912449387
86-87	22.482675093248	27.739212842917382	27.298531631591366	22.479580432243253
88-89	22.54201129412484	27.762887816878308	27.284993751711177	22.410107137285674
90-91	22.515732746790135	27.854133974896506	27.32242145930383	22.307711819009526
92-93	22.544701580599746	27.779739571162516	27.35869498305286	22.316863865184878
94-95	22.351428439430812	27.881947362040304	27.39516576371489	22.37145843481399
96-97	22.387124594936132	28.007683267761426	27.495667868412998	22.109524268889448
98-99	23.593245115716186	29.864340690053798	27.59698108609533	18.945433108134687
100	22.444549480944627	30.87049535427847	21.507116580154463	25.177838584622442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2602.0
1	2265.5
2	2690.5
3	3716.5
4	5166.0
5	8342.0
6	10970.5
7	12867.5
8	14260.5
9	14300.5
10	13285.0
11	12243.0
12	13354.0
13	17747.0
14	27974.0
15	41415.0
16	52257.5
17	58635.0
18	59566.0
19	55909.0
20	49970.5
21	43524.5
22	39143.0
23	39420.0
24	44483.5
25	54069.5
26	67205.5
27	81770.0
28	96646.5
29	114566.5
30	132033.5
31	148035.5
32	166691.0
33	184022.5
34	201099.5
35	219145.5
36	238174.0
37	255969.5
38	267758.0
39	275913.0
40	286817.0
41	296636.0
42	303027.5
43	311297.0
44	319507.5
45	327691.0
46	333517.0
47	335079.5
48	334258.5
49	331350.0
50	326261.5
51	318979.5
52	308563.5
53	294067.5
54	279143.0
55	261636.0
56	241638.0
57	220828.5
58	198050.0
59	173622.5
60	145219.5
61	117085.0
62	92855.5
63	71798.5
64	53741.5
65	39895.5
66	30120.5
67	22939.0
68	17358.0
69	12624.0
70	9129.5
71	6721.5
72	4860.0
73	3301.0
74	2239.5
75	1520.5
76	976.0
77	605.0
78	396.0
79	260.5
80	181.0
81	127.0
82	93.0
83	70.5
84	45.5
85	28.0
86	21.0
87	14.5
88	7.0
89	3.0
90	3.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	9.180286205085904E-4
4	0.010134552797456673
5	0.011668626939622345
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.2079625995451778E-5
22-23	0.0
24-25	0.0062045293184853165
26-27	0.04559744161185657
28-29	0.07945767311339422
30-31	0.07184019805328075
32-33	0.03336134068431267
34-35	0.003247839532885257
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.889561022395859E-4
56-57	4.920565022576864E-4
58-59	1.3215485562379372E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.2153214957629209E-4
72-73	0.012752902908318008
74-75	0.027894880212811755
76-77	0.0023841381585356794
78-79	0.0
80-81	0.0
82-83	0.008058378801363512
84-85	0.044817239457988524
86-87	0.037396528050252155
88-89	0.009522507318882248
90-91	8.328704592659716E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	524.0
22-23	1136.0
24-25	2556.0
26-27	3630.0
28-29	6987.0
30-31	15910.0
32-33	20478.0
34-35	28826.0
36-37	40089.0
38-39	46840.0
40-41	51735.0
42-43	60747.0
44-45	74081.0
46-47	72754.0
48-49	63216.0
50-51	53233.0
52-53	49294.0
54-55	51952.0
56-57	54068.0
58-59	54072.0
60-61	53893.0
62-63	54677.0
64-65	57596.0
66-67	60708.0
68-69	61809.0
70-71	60128.0
72-73	57915.0
74-75	58732.0
76-77	58316.0
78-79	61811.0
80-81	63266.0
82-83	60365.0
84-85	61579.0
86-87	64865.0
88-89	69026.0
90-91	72488.0
92-93	77469.0
94-95	92857.0
96-97	268844.0
98-99	511968.0
100-101	5598054.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.73421664536576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71424437000842	74.28417757608206
2	7.782374156699571	13.033020073047554
3	1.9270867961040143	4.840893098381921
4	0.7483764153612957	2.5065885158457992
5	0.3314281439509918	1.3875938003981911
6	0.1694433970379708	0.8512926070022506
7	0.09928481628149033	0.5819475421276754
8	0.06171666189503651	0.4134237070198221
9	0.040732266573574184	0.3069615990355606
>10	0.12259556425858432	1.5251007818711084
>50	0.00214569179891667	0.11846103399354235
>100	4.530477564309881E-4	0.06518196805121027
>500	9.493819173236469E-5	0.0508770920787945
>1k	2.3734081936708005E-5	0.03448060506461041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.831729581624159E-5	0.0	0.0	0.0
3	0.0	4.831729581624159E-5	0.0	0.0	0.0
4	0.0	1.449518874487248E-4	0.0	0.0	0.0
5	0.0	1.6911053535684556E-4	0.0	0.0	0.0
6	1.2079323954060398E-4	1.6911053535684556E-4	0.0	0.0	0.0
7	1.2079323954060398E-4	1.6911053535684556E-4	0.0	0.0	0.0
8	1.328725634946644E-4	2.1742783117308716E-4	0.0	0.0	2.4158647908120795E-5
9	1.328725634946644E-4	3.5030039466775155E-4	0.0	0.0	2.4158647908120795E-5
10-11	1.328725634946644E-4	3.5030039466775155E-4	0.0	0.0	2.4158647908120795E-5
12-13	1.328725634946644E-4	3.623797186218119E-4	0.0	0.0	2.4158647908120795E-5
14-15	1.5703121140278516E-4	4.7109363420835555E-4	0.0	0.0	4.22776338392114E-5
16-17	2.657451269893288E-4	4.831729581624159E-4	0.0	0.0	6.039661977030199E-5
18-19	2.657451269893288E-4	5.435695779327179E-4	0.0	0.0	6.039661977030199E-5
20-21	2.71784788966359E-4	6.039661977030199E-4	0.0	0.0	6.039661977030199E-5
22-23	2.8386411292041937E-4	6.402041695652012E-4	0.0	1.8118985931090596E-5	6.64362817473322E-5
24-25	2.899037748974496E-4	6.824818034044124E-4	0.0	9.059492965545298E-5	7.24759437243624E-5
26-27	3.0198309885150996E-4	6.885214653814427E-4	0.0	2.174278311730872E-4	7.24759437243624E-5
28-29	3.0198309885150996E-4	6.885214653814427E-4	0.0	7.247594372436239E-4	7.24759437243624E-5
30-31	3.0802276082854017E-4	7.609974091058051E-4	0.0	0.002458142424651291	7.24759437243624E-5
32-33	3.2614174675963076E-4	7.670370710828353E-4	0.0	0.005526290708982633	8.455526767842278E-5
34-35	3.623797186218119E-4	8.878303106234393E-4	0.0	0.009385634712304929	9.663459163248318E-5
36-37	3.623797186218119E-4	0.0010086235501640434	0.0	0.01601114390110706	9.663459163248318E-5
38-39	3.623797186218119E-4	0.0010388218600491943	0.0	0.03229407259118047	9.663459163248318E-5
40-41	3.865383665299327E-4	0.0011958530714519795	0.0	0.04886086539417431	1.0267425360951338E-4
42-43	3.865383665299327E-4	0.0012441703672682212	0.0	0.05882630765627414	1.0871391558654358E-4
44-45	3.9861769048399315E-4	0.0014072412406480363	0.0	0.07269337155553549	1.0871391558654358E-4
46-47	3.9861769048399315E-4	0.0015099154942575497	0.0	0.08810054925893951	1.0871391558654358E-4
48-49	4.0465735246102336E-4	0.0015099154942575497	0.0	0.10140592459433705	1.0871391558654358E-4
50-51	4.288160003691441E-4	0.0017092243394995464	0.0	0.11529110747952948	1.0871391558654358E-4
52-53	4.4089532432320454E-4	0.0017333829874076672	0.0	0.14938499933986493	1.2683290151763418E-4
54-55	4.4693498630023475E-4	0.0017696209592698484	0.0	0.19474286078736175	1.449518874487248E-4
56-57	4.4693498630023475E-4	0.0018602158889253013	0.0	0.2428729270823154	1.449518874487248E-4
58-59	4.5297464827726496E-4	0.0018722952128793617	0.0	0.28359836779343006	1.449518874487248E-4
60-61	5.012919440935066E-4	0.0018843745368334222	0.0	0.3184894950347335	1.449518874487248E-4
62-63	5.556489018867783E-4	0.0019568504805577845	0.0	0.34549282373403556	1.449518874487248E-4
64-65	5.556489018867783E-4	0.0021923972976619624	0.0	0.35049366385101655	1.449518874487248E-4
66-67	5.677282258408387E-4	0.0022286352695241435	0.0	0.355186481207169	1.449518874487248E-4
68-69	5.677282258408387E-4	0.0022950715512714756	0.0	0.3600242504507702	1.449518874487248E-4
70-71	5.918868737489595E-4	0.002307150875225536	0.0	0.3647351867928537	1.449518874487248E-4
72-73	6.402041695652011E-4	0.0023192301991795967	0.0	0.368582451472222	1.9326918326496636E-4
74-75	6.402041695652011E-4	0.002373587156972868	0.0	0.3711372284885057	1.9326918326496636E-4
76-77	6.402041695652011E-4	0.0024762614105823815	0.0	0.3735772519272259	1.9326918326496636E-4
78-79	6.402041695652011E-4	0.002524578706398623	0.0	0.3754072695062661	1.9326918326496636E-4
80-81	6.402041695652011E-4	0.002524578706398623	0.0	0.37604747367583125	2.1742783117308716E-4
82-83	6.462438315422312E-4	0.0025306183683756534	0.0	0.3763615360986369	2.5366580303526836E-4
84-85	6.522834935192615E-4	0.002578935664191895	0.0	0.37651252764806264	2.5970546501229857E-4
86-87	6.522834935192615E-4	0.002609133974077046	0.0	0.3765548052819018	2.657451269893288E-4
88	6.522834935192615E-4	0.0027178478896635896	0.0	0.37656084494387887	2.899037748974496E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13210	0.0	50.43013	1
GTATCAA	24250	0.0	38.44583	1
ATCAACG	29580	0.0	30.923346	3
TCAACGC	29705	0.0	30.83601	4
TATCAAC	30330	0.0	30.3285	2
CAACGCA	30320	0.0	30.207804	5
AACGCAG	30730	0.0	29.870275	6
ACGCAGA	35220	0.0	26.086649	7
CGCAGAG	35595	0.0	25.775654	8
GTGGTAT	6175	0.0	24.12091	1
GCAGAGT	40425	0.0	22.66412	9
TGGTATC	6340	0.0	22.61301	2
GTACATG	28180	0.0	21.599138	1
TACATGG	27510	0.0	21.516659	2
GAGTACT	24860	0.0	20.688448	12-13
ACATGGG	28075	0.0	20.334478	3
CAGAGTA	39600	0.0	19.673971	10-11
AGAGTAC	37185	0.0	19.128317	10-11
GTACTTT	27780	0.0	18.452732	14-15
CATGGGG	20425	0.0	17.988916	4
>>END_MODULE