FastQCFastQC Report
Fri 27 May 2016
SRR2138654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8496649
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130700.052.2721
GTATCAA245100.039.3877451
TCAACGC297850.031.662584
ATCAACG299000.031.5845763
TATCAAC306550.031.1004772
CAACGCA305350.030.8960255
AACGCAG313500.030.1583776
ACGCAGA359300.026.2782847
CGCAGAG361350.026.141078
GTGGTAT61100.024.0056691
TGGTATC58850.022.6643282
GCAGAGT416800.022.5604529
TACATGG290300.020.8896562
GTACATG294900.020.854671
GAGTACT252100.020.68224112-13
ACATGGG295750.019.9971663
CAGAGTA404250.019.88256310-11
AGAGTAC378950.019.4844510-11
GTACTTT276200.018.77719114-15
AGTACTT267000.018.3557412-13
Produced by FastQC (version 0.11.5)