##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8496649
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.770468451739035	33.0	33.0	33.0	33.0	33.0
2	32.7668420809192	33.0	33.0	33.0	33.0	33.0
3	32.76364293735095	33.0	33.0	33.0	33.0	33.0
4	36.70736369126229	37.0	37.0	37.0	37.0	37.0
5	36.72396529502396	37.0	37.0	37.0	37.0	37.0
6	36.73189995255777	37.0	37.0	37.0	37.0	37.0
7	36.71932122887505	37.0	37.0	37.0	37.0	37.0
8	36.7106239177351	37.0	37.0	37.0	37.0	37.0
9	36.707852942966106	37.0	37.0	37.0	37.0	37.0
10-11	36.67802630189855	37.0	37.0	37.0	37.0	37.0
12-13	36.65655295399398	37.0	37.0	37.0	37.0	37.0
14-15	38.87777346104329	40.0	38.5	40.0	37.0	40.0
16-17	38.82849621068259	40.0	37.0	40.0	37.0	40.0
18-19	38.85759633003552	40.0	38.5	40.0	37.0	40.0
20-21	38.893294322569275	40.0	40.0	40.0	37.0	40.0
22-23	38.92152594961491	40.0	40.0	40.0	37.0	40.0
24-25	38.93052757228052	40.0	40.0	40.0	37.0	40.0
26-27	38.83565815499577	40.0	40.0	40.0	37.0	40.0
28-29	38.76232207997717	40.0	37.0	40.0	37.0	40.0
30-31	38.6976553707462	40.0	37.0	40.0	37.0	40.0
32-33	38.378179268330356	40.0	37.0	40.0	37.0	40.0
34-35	38.51478813185334	40.0	37.0	40.0	37.0	40.0
36-37	38.483654470283525	40.0	37.0	40.0	37.0	40.0
38-39	38.514258158635464	40.0	37.0	40.0	37.0	40.0
40-41	38.47937853031378	40.0	37.0	40.0	37.0	40.0
42-43	38.17891136440306	40.0	37.0	40.0	37.0	40.0
44-45	38.08041735089439	40.0	37.0	40.0	35.0	40.0
46-47	38.106029631431085	40.0	37.0	40.0	35.0	40.0
48-49	37.973053198179166	40.0	37.0	40.0	33.0	40.0
50-51	38.076085830760526	40.0	37.0	40.0	37.0	40.0
52-53	38.06354313019075	40.0	37.0	40.0	37.0	40.0
54-55	38.00741428859839	40.0	37.0	40.0	33.0	40.0
56-57	37.8983354265764	40.0	37.0	40.0	33.0	40.0
58-59	37.77257090083434	40.0	37.0	40.0	33.0	40.0
60-61	37.53333672235945	40.0	37.0	40.0	33.0	40.0
62-63	37.34553199842628	37.0	37.0	40.0	33.0	40.0
64-65	37.175105263188286	37.0	37.0	40.0	33.0	40.0
66-67	36.937102467561616	37.0	37.0	40.0	33.0	40.0
68-69	36.66344169799631	37.0	37.0	40.0	33.0	40.0
70-71	36.20861521870262	37.0	37.0	40.0	33.0	40.0
72-73	36.099804719735985	37.0	37.0	37.0	33.0	40.0
74-75	35.86179461321265	37.0	37.0	37.0	33.0	40.0
76-77	33.81808876682909	35.0	33.0	37.0	27.0	37.0
78-79	35.23772370993743	37.0	33.0	37.0	33.0	37.0
80-81	35.55032541360397	37.0	35.0	37.0	33.0	37.0
82-83	35.549682812261864	37.0	37.0	37.0	33.0	37.0
84-85	35.442456705080374	37.0	33.0	37.0	33.0	37.0
86-87	35.0051015628686	37.0	33.0	37.0	33.0	37.0
88-89	34.92631964655799	37.0	33.0	37.0	33.0	37.0
90-91	35.08844845667699	37.0	33.0	37.0	33.0	37.0
92-93	35.079300112021244	37.0	33.0	37.0	33.0	37.0
94-95	35.04933295627881	37.0	33.0	37.0	33.0	37.0
96-97	35.0079914423394	37.0	33.0	37.0	33.0	37.0
98-99	34.97615798741754	37.0	33.0	37.0	33.0	37.0
100	34.91742658219943	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	32.0
21	147.0
22	611.0
23	1927.0
24	4716.0
25	9722.0
26	17840.0
27	29691.0
28	44748.0
29	63689.0
30	87878.0
31	118347.0
32	157964.0
33	216174.0
34	305781.0
35	470380.0
36	915839.0
37	2515177.0
38	3406850.0
39	129132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.142802415399295	20.00625187647507	14.494608403854272	23.356337304271367
2	14.713741852817504	20.868968460389503	41.819557333720624	22.59773235307237
3	18.273532401559173	25.997325267629183	30.426416554199463	25.302725776612178
4	12.364927495802295	18.089695805473703	38.9325795415689	30.612797157155107
5	13.319817551064078	37.71624790615822	35.34927534918354	13.614659193594159
6	29.740042221351032	37.88644205497956	18.761643560890885	13.611872162778527
7	26.431643816285693	32.985027391386886	22.732079435080816	17.851249357246605
8	24.509309493660382	36.45658423691505	21.00288007660432	18.031226192820252
9	25.636636278608187	16.92215366316768	21.221495674353502	36.219714383870624
10-11	24.114612713788695	26.936725290170276	28.643480506256054	20.305181489784975
12-13	25.16189617812858	24.523150244290427	28.80028938467389	21.51466419290711
14-15	22.234347917631997	25.889435941157508	26.816707386641486	25.05950875456901
16-17	21.45601165824315	29.042614329484483	27.46590449952681	22.035469512745554
18-19	21.43083114296001	27.967037357904275	29.559447495124253	21.042684004011463
20-21	22.443228562369313	27.0704853021937	29.473156868796075	21.013129266640913
22-23	22.06969314211788	27.041910450122337	29.337621120754747	21.55077528700503
24-25	21.792447848584686	27.208086500362892	29.44895537047183	21.550510280580585
26-27	21.68828580516212	27.381904585785087	29.356455775816144	21.57335383323665
28-29	21.56404530477366	27.506341681216117	29.402345008332237	21.527268005677985
30-31	22.010102094175625	27.25747777762429	29.273811476140498	21.45860865205959
32-33	21.7942431187248	27.37608612296943	29.13048474688886	21.699186011416906
34-35	21.854604866705987	27.48874811588981	29.18596240212085	21.47068461528335
36-37	21.882544669983815	27.438226258451024	29.123433160330837	21.555795911234327
38-39	21.65015469365758	27.55783401696556	29.209921445810462	21.5820898435664
40-41	21.947832551817708	27.451526049081547	29.008873912600784	21.59176748649995
42-43	21.98966390555819	27.50829366844605	28.72558405096938	21.77645837502638
44-45	22.062884607961173	27.679354532475838	28.51843466245957	21.739326197103416
46-47	22.090276439397307	27.666504825582255	28.217604603699364	22.025614131321067
48-49	22.05723045445917	27.792382426585483	28.140990534529315	22.00939658442603
50-51	21.97917478302615	27.964542714881404	28.029829663272665	22.026452838819782
52-53	22.145552011599325	27.885957709538694	27.912248494732356	22.056241784129625
54-55	22.17903569675775	27.76588174498692	27.950593918722568	22.10448863953276
56-57	22.124196369436238	27.914940374095586	27.88172527923283	22.079137977235348
58-59	22.01854673729999	27.965252643743348	27.942263009426462	22.0739376095302
60-61	22.220309638174097	27.91552215323325	27.868815332280278	21.99535287631237
62-63	22.08494294054175	28.021753021074453	27.8228306804454	22.070473357938397
64-65	22.07046269044024	28.007795715831215	27.785347880614907	22.13639371311364
66-67	22.156570342362336	28.06754401330473	27.6075597717096	22.168325872623335
68-69	22.15350061077667	28.035589613424648	27.66964753625198	22.141262239546705
70-71	22.14845691239407	27.771471725189667	27.684050326544835	22.396021035871428
72-73	22.152676809274	27.81255579688574	27.696853462060577	22.337913931779678
74-75	22.043500969100087	27.976635817806976	27.60166735901219	22.378195854080744
76-77	22.24576632969269	27.880826246846986	27.476079854738888	22.397327568721437
78-79	22.287106948895982	27.991199235706894	27.362726182168796	22.35896763322833
80-81	22.185750925879972	27.96856063812281	27.454264060170676	22.391424375826542
82-83	22.24178698461305	27.968176869250993	27.559560633371195	22.230475512764762
84-85	22.3809125380828	27.927998060925578	27.473599375263806	22.217490025727816
86-87	22.223921754038678	27.969915530851182	27.523625244966475	22.282537470143662
88-89	22.320321765917697	28.01145687887821	27.458429028153493	22.2097923270506
90-91	22.30209431839672	28.017488978974576	27.54840342954742	22.132013273081288
92-93	22.29042892147515	27.981308542608645	27.51138228272033	22.21688025319587
94-95	22.15882626833142	28.110350845811656	27.525745343817658	22.205077542039266
96-97	22.215234958965834	28.17484844040587	27.68935089727963	21.920565703348668
98-99	23.28388933018378	30.0870098381223	27.88655106730652	18.742549764387405
100	22.25935891840637	31.107207309981	21.80098498589015	24.832448785722484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2540.0
1	2230.5
2	2733.0
3	3803.0
4	5164.5
5	8267.0
6	10901.5
7	12824.0
8	14288.0
9	14585.0
10	14000.0
11	13169.5
12	14221.0
13	18503.5
14	28620.0
15	42100.0
16	52975.0
17	59316.0
18	60469.5
19	56944.0
20	51032.5
21	44570.0
22	40213.0
23	40720.5
24	46150.5
25	56456.5
26	70827.0
27	86914.5
28	103174.5
29	120780.0
30	137880.5
31	155136.0
32	174629.5
33	194236.5
34	214838.5
35	234077.0
36	254473.0
37	273743.5
38	284498.0
39	292729.0
40	305742.0
41	317787.0
42	325632.5
43	333469.5
44	339852.0
45	346098.5
46	350228.0
47	351137.0
48	349184.5
49	343414.5
50	335822.5
51	324251.5
52	308397.0
53	292079.5
54	274257.0
55	254397.0
56	233581.0
57	210727.0
58	188168.0
59	165200.0
60	139003.5
61	113196.5
62	89473.5
63	68732.0
64	52088.5
65	39218.0
66	29953.0
67	23196.5
68	17717.5
69	12385.5
70	8574.0
71	6211.0
72	4353.5
73	3048.0
74	2089.5
75	1406.0
76	956.5
77	600.5
78	378.0
79	249.0
80	162.5
81	103.5
82	70.0
83	54.0
84	42.0
85	32.0
86	26.0
87	20.0
88	13.5
89	7.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0011533958858368752
4	0.010992568952771852
5	0.01061594988800879
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.3539270565083727E-5
22-23	0.0
24-25	0.006139602674111891
26-27	0.046624996238732466
28-29	0.08182542820628798
30-31	0.07448051814750328
32-33	0.03447529266750552
34-35	0.003184310186205058
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.846481608883996E-4
56-57	4.6167486278478044E-4
58-59	1.2919152203891095E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.7612959019115346E-4
72-73	0.013256299730332306
74-75	0.030065290630569043
76-77	0.002554415647572826
78-79	0.0
80-81	0.0
82-83	0.007727958757629134
84-85	0.04624870017586289
86-87	0.03888070546984755
88-89	0.009464271715507946
90-91	7.395787256037939E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	125.0
20-21	532.0
22-23	1212.0
24-25	2774.0
26-27	4048.0
28-29	7722.0
30-31	18298.0
32-33	22826.0
34-35	31596.0
36-37	44283.0
38-39	50918.0
40-41	55027.0
42-43	64856.0
44-45	78459.0
46-47	76349.0
48-49	65599.0
50-51	55627.0
52-53	50506.0
54-55	53826.0
56-57	57481.0
58-59	56549.0
60-61	57440.0
62-63	57560.0
64-65	59827.0
66-67	63619.0
68-69	63166.0
70-71	60826.0
72-73	58298.0
74-75	59822.0
76-77	57930.0
78-79	61175.0
80-81	63129.0
82-83	60768.0
84-85	61968.0
86-87	64620.0
88-89	69125.0
90-91	73633.0
92-93	78047.0
94-95	95224.0
96-97	273936.0
98-99	525049.0
100-101	5732874.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.41497662211911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.41599338146756	78.13295954325197
2	6.867771060267761	11.869565512382094
3	1.5727004703968928	4.077146231488296
4	0.5529705224272286	1.9113973907307977
5	0.23887187302379767	1.032105366151664
6	0.12493884669925011	0.6477952510026133
7	0.06786886962874632	0.4105420747636434
8	0.042027359238281804	0.2905434612852419
9	0.026585436543752214	0.20676418896754611
>10	0.08768825948807829	1.1323985934004104
>50	0.0018862247583091682	0.10623664879704196
>100	5.751429322494646E-4	0.08440893752893894
>500	1.0212816651195546E-4	0.05715297601387623
>1k	2.042496157380579E-5	0.04098382423580628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1769345773845666E-5
2	0.0	0.0	0.0	1.1769345773845666E-5	1.1769345773845666E-5
3	0.0	0.0	0.0	2.3538691547691332E-5	1.1769345773845666E-5
4	0.0	1.1769345773845666E-5	0.0	3.5308037321536996E-5	1.1769345773845666E-5
5	0.0	1.1769345773845666E-5	0.0	4.7077383095382664E-5	1.1769345773845666E-5
6	4.7077383095382664E-5	2.3538691547691332E-5	0.0	5.8846728869228324E-5	1.1769345773845666E-5
7	4.7077383095382664E-5	3.5308037321536996E-5	0.0	5.8846728869228324E-5	1.1769345773845666E-5
8	4.7077383095382664E-5	4.7077383095382664E-5	0.0	5.8846728869228324E-5	2.3538691547691332E-5
9	4.7077383095382664E-5	1.2946280351230232E-4	0.0	8.238542041691965E-5	2.3538691547691332E-5
10-11	4.7077383095382664E-5	1.2946280351230232E-4	0.0	1.1180878485153383E-4	2.3538691547691332E-5
12-13	4.7077383095382664E-5	1.2946280351230232E-4	0.0	1.3534747639922515E-4	2.3538691547691332E-5
14-15	4.7077383095382664E-5	2.2361756970306764E-4	0.0	2.0007887815537631E-4	2.3538691547691332E-5
16-17	4.7077383095382664E-5	2.353869154769133E-4	0.0	2.1184822392922198E-4	3.5308037321536996E-5
18-19	4.7077383095382664E-5	2.5892560702460465E-4	0.0	2.47156261250759E-4	3.5308037321536996E-5
20-21	4.7077383095382664E-5	2.8246429857229597E-4	0.0	2.5892560702460465E-4	3.5308037321536996E-5
22-23	4.7077383095382664E-5	3.0011831723306446E-4	0.0	2.7657962568537314E-4	4.1192710208459826E-5
24-25	4.7077383095382664E-5	3.354263545546015E-4	0.0	3.295416816676786E-4	4.7077383095382664E-5
26-27	4.7077383095382664E-5	3.413110274415243E-4	0.0	4.825431767276723E-4	4.7077383095382664E-5
28-29	4.7077383095382664E-5	3.413110274415243E-4	0.0	0.0010474717738722644	5.2962055982305494E-5
30-31	4.7077383095382664E-5	3.8250373764998414E-4	0.0	0.002877605041705265	5.8846728869228324E-5
32-33	4.7077383095382664E-5	4.0015775631075263E-4	0.0	0.006402524100972042	5.8846728869228324E-5
34-35	4.7077383095382664E-5	5.119665411622864E-4	0.0	0.010986684279884928	5.8846728869228324E-5
36-37	4.7077383095382664E-5	6.002366344661289E-4	0.0	0.01794825230511464	5.8846728869228324E-5
38-39	4.7077383095382664E-5	6.355446717876659E-4	0.0	0.03582000386269928	5.8846728869228324E-5
40-41	4.7077383095382664E-5	7.179300922045856E-4	0.0	0.05464507242796543	5.8846728869228324E-5
42-43	4.7077383095382664E-5	7.41468783752277E-4	0.0	0.06582006624023187	5.8846728869228324E-5
44-45	4.7077383095382664E-5	8.415082228299651E-4	0.0	0.08071417331703357	5.8846728869228324E-5
46-47	4.7077383095382664E-5	9.180089703599619E-4	0.0	0.09620851702830138	5.8846728869228324E-5
48-49	4.7077383095382664E-5	9.180089703599619E-4	0.0	0.11064950429281002	5.8846728869228324E-5
50-51	4.7077383095382664E-5	9.474323347945761E-4	0.0	0.1246138330534779	5.8846728869228324E-5
52-53	4.7077383095382664E-5	9.53317007681499E-4	0.0	0.1652769227021147	5.8846728869228324E-5
54-55	4.7077383095382664E-5	9.709710263422675E-4	0.0	0.21775643550769253	5.8846728869228324E-5
56-57	5.8846728869228324E-5	9.88625045003036E-4	0.0	0.27201900419800795	5.8846728869228324E-5
58-59	5.8846728869228324E-5	0.0010062790636638044	0.0	0.3122701667445601	5.8846728869228324E-5
60-61	5.8846728869228324E-5	0.0010415871009853413	0.0	0.3389630429596421	5.8846728869228324E-5
62-63	7.061607464307399E-5	0.0010651257925330328	0.0	0.35789992030975976	7.061607464307399E-5
64-65	7.061607464307399E-5	0.001235781306253795	0.0	0.362607658619298	7.061607464307399E-5
66-67	7.061607464307399E-5	0.001235781306253795	0.0	0.3670388173031509	7.061607464307399E-5
68-69	7.061607464307399E-5	0.0012475506520276405	0.0	0.3717406709398023	7.061607464307399E-5
70-71	7.650074752999682E-5	0.0012475506520276405	0.0	0.37562455504517134	7.061607464307399E-5
72-73	8.238542041691965E-5	0.0012475506520276405	0.0	0.37895527989916966	8.238542041691965E-5
74-75	8.238542041691965E-5	0.0012475506520276405	0.0	0.38149745858632034	8.238542041691965E-5
76-77	8.827009330384248E-5	0.0012652046706884091	0.0	0.3841985234414179	8.238542041691965E-5
78-79	9.415476619076533E-5	0.0013063973808968689	0.0	0.38565203764448786	8.238542041691965E-5
80-81	9.415476619076533E-5	0.0013181667266707144	0.0	0.3861463501669894	8.827009330384248E-5
82-83	9.415476619076533E-5	0.0013358207453314829	0.0	0.38638762175535324	9.415476619076533E-5
84-85	9.415476619076533E-5	0.0013417054182184058	0.0	0.3865170845588655	9.415476619076533E-5
86-87	1.0003943907768816E-4	0.0013534747639922515	0.0	0.38656416194196086	9.415476619076533E-5
88	1.0592411196461099E-4	0.0014005521470876342	0.0	0.3865759312877347	9.415476619076533E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13070	0.0	52.272	1
GTATCAA	24510	0.0	39.387745	1
TCAACGC	29785	0.0	31.66258	4
ATCAACG	29900	0.0	31.584576	3
TATCAAC	30655	0.0	31.100477	2
CAACGCA	30535	0.0	30.896025	5
AACGCAG	31350	0.0	30.158377	6
ACGCAGA	35930	0.0	26.278284	7
CGCAGAG	36135	0.0	26.14107	8
GTGGTAT	6110	0.0	24.005669	1
TGGTATC	5885	0.0	22.664328	2
GCAGAGT	41680	0.0	22.560452	9
TACATGG	29030	0.0	20.889656	2
GTACATG	29490	0.0	20.85467	1
GAGTACT	25210	0.0	20.682241	12-13
ACATGGG	29575	0.0	19.997166	3
CAGAGTA	40425	0.0	19.882563	10-11
AGAGTAC	37895	0.0	19.48445	10-11
GTACTTT	27620	0.0	18.777191	14-15
AGTACTT	26700	0.0	18.35574	12-13
>>END_MODULE