FastQCFastQC Report
Fri 27 May 2016
SRR2138653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577652
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120150.050.993881
GTATCAA215650.037.2020871
ATCAACG254250.030.9661393
TCAACGC267300.029.4849074
TATCAAC272850.029.027522
CAACGCA272200.028.9034565
AACGCAG273200.028.8422556
ACGCAGA273650.028.7792267
CGCAGAG317100.024.849278
CCCACGA48750.022.24799782-83
GTGGTAT56350.022.1247751
AGACCGA51700.021.977388-89
CACGAGA49800.021.94176984-85
ACCGAGG54050.021.87917790-91
GCAGAGT361400.021.626119
CGAGACC51750.021.54725386-87
TACATGG248800.020.7131822
TGGTATC56050.020.6435152
TCCGAGC51400.020.61376676-77
CGAGCCC53750.020.40056678-79
Produced by FastQC (version 0.11.5)