FastQCFastQC Report
Fri 27 May 2016
SRR2138652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8103841
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114200.050.549711
ATCTCGT66700.038.29556794
GTATCAA200350.035.946151
TATCTCG83450.030.60891794
ATCAACG239350.029.4701443
TCAACGC239650.029.4687064
CAACGCA244500.028.8620595
TATCAAC246350.028.844982
AACGCAG250600.028.2264296
TATGCCG36450.024.96325586-87
ACGCAGA290600.024.2812147
GTGGTAT59700.024.2288421
CCGTCTT38900.024.16027690-91
GCCGTCT40700.024.11051890-91
CGCAGAG293550.024.0371998
TGGTATC55900.023.8494572
ATGCCGT41950.022.93085588-89
TGCCGTC40750.022.8059188-89
TCTCGTA43050.021.96344682-83
CGCTATC126950.021.85945194
Produced by FastQC (version 0.11.5)