FastQCFastQC Report
Fri 27 May 2016
SRR2138651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8475193
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129750.048.6716581
GTATCAA228200.037.427751
ATCAACG284900.029.3653373
CAACGCA289200.028.9114115
TCAACGC290000.028.8193364
AACGCAG292250.028.7677926
TATCAAC297100.028.5039482
ACGCAGA326600.025.637727
CGCAGAG331200.025.2687688
GCAGAGT380200.021.7878729
TACATGG267900.020.3098352
GAGTACT239250.020.01160612-13
ACATGGG268700.019.7732853
GTACATG291500.019.309181
AGAGTAC358800.019.04737910-11
CAGAGTA380000.019.04497310-11
CATGGGG182150.017.7914094
GTACTTT272000.017.46172314-15
AGTACTT265650.016.94760112-13
GTGGTAT66200.016.7472611
Produced by FastQC (version 0.11.5)