##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8475193
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.774635220696446	33.0	33.0	33.0	33.0	33.0
2	32.772476449798845	33.0	33.0	33.0	33.0	33.0
3	32.7723411136478	33.0	33.0	33.0	33.0	33.0
4	36.715955849029044	37.0	37.0	37.0	37.0	37.0
5	36.73094689407073	37.0	37.0	37.0	37.0	37.0
6	36.740763779656696	37.0	37.0	37.0	37.0	37.0
7	36.72869184217988	37.0	37.0	37.0	37.0	37.0
8	36.721700614959445	37.0	37.0	37.0	37.0	37.0
9	36.719390815052826	37.0	37.0	37.0	37.0	37.0
10-11	36.69062226665517	37.0	37.0	37.0	37.0	37.0
12-13	36.670654343800784	37.0	37.0	37.0	37.0	37.0
14-15	38.90518398814045	40.0	40.0	40.0	37.0	40.0
16-17	38.85827136915938	40.0	37.0	40.0	37.0	40.0
18-19	38.890893104145235	40.0	40.0	40.0	37.0	40.0
20-21	38.92819511923527	40.0	40.0	40.0	37.0	40.0
22-23	38.95137792978079	40.0	40.0	40.0	37.0	40.0
24-25	38.95941528698027	40.0	40.0	40.0	37.0	40.0
26-27	38.86815619054289	40.0	40.0	40.0	37.0	40.0
28-29	38.79157284837001	40.0	38.5	40.0	37.0	40.0
30-31	38.73121572614698	40.0	37.0	40.0	37.0	40.0
32-33	38.42302764513432	40.0	37.0	40.0	37.0	40.0
34-35	38.55275195363427	40.0	37.0	40.0	37.0	40.0
36-37	38.53037639017496	40.0	37.0	40.0	37.0	40.0
38-39	38.556297665303404	40.0	37.0	40.0	37.0	40.0
40-41	38.537247176683536	40.0	37.0	40.0	37.0	40.0
42-43	38.25194954119675	40.0	37.0	40.0	37.0	40.0
44-45	38.163132664296924	40.0	37.0	40.0	37.0	40.0
46-47	38.203927921166795	40.0	37.0	40.0	37.0	40.0
48-49	38.08023114399165	40.0	37.0	40.0	37.0	40.0
50-51	38.18046719284276	40.0	37.0	40.0	37.0	40.0
52-53	38.17411625163791	40.0	37.0	40.0	37.0	40.0
54-55	38.12831546285787	40.0	37.0	40.0	37.0	40.0
56-57	38.02316425077497	40.0	37.0	40.0	35.0	40.0
58-59	37.90549404140223	40.0	37.0	40.0	33.0	40.0
60-61	37.68076090917665	40.0	37.0	40.0	33.0	40.0
62-63	37.50081635251847	38.5	37.0	40.0	33.0	40.0
64-65	37.341374746466705	37.0	37.0	40.0	33.0	40.0
66-67	37.11834736003616	37.0	37.0	40.0	33.0	40.0
68-69	36.84918384544413	37.0	37.0	40.0	33.0	40.0
70-71	36.409580628959276	37.0	37.0	40.0	33.0	40.0
72-73	36.302365200603035	37.0	37.0	38.5	33.0	40.0
74-75	36.063604608660754	37.0	37.0	37.0	33.0	40.0
76-77	34.03276637928715	35.0	33.0	37.0	30.0	37.0
78-79	35.409357136378745	37.0	33.0	37.0	33.0	37.0
80-81	35.697794161071144	37.0	37.0	37.0	33.0	37.0
82-83	35.68163818294795	37.0	37.0	37.0	33.0	37.0
84-85	35.57129728251197	37.0	37.0	37.0	33.0	37.0
86-87	35.12614068336411	37.0	33.0	37.0	33.0	37.0
88-89	35.053625608138134	37.0	33.0	37.0	33.0	37.0
90-91	35.22365086361877	37.0	33.0	37.0	33.0	37.0
92-93	35.21543460954829	37.0	33.0	37.0	33.0	37.0
94-95	35.18664158905856	37.0	33.0	37.0	33.0	37.0
96-97	35.14682021423319	37.0	33.0	37.0	33.0	37.0
98-99	35.11284170055633	37.0	33.0	37.0	33.0	37.0
100	35.05712882962418	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	18.0
21	148.0
22	583.0
23	1733.0
24	4258.0
25	9114.0
26	16258.0
27	26949.0
28	40618.0
29	58364.0
30	79471.0
31	107671.0
32	144875.0
33	198492.0
34	284299.0
35	438740.0
36	863331.0
37	2461569.0
38	3593460.0
39	145240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3892639377062	20.79716650700462	14.409772143242048	23.403797412047137
2	14.282022840069835	21.03594573008544	42.523326607429475	22.158704822415253
3	17.50497780856071	26.927988587765476	31.07136599326381	24.495667610410006
4	11.752927024348661	18.42929674713186	40.16666379524176	29.65111243327772
5	12.578278147445893	38.41288710718141	36.167371624310206	12.84146312106249
6	28.69357665365261	39.082779589798136	19.101500107431182	13.12214364911808
7	25.491348692590243	33.36095119013809	23.742291178501777	17.405408938769888
8	23.98214412344356	36.80589928748525	21.548382438016457	17.663574151054732
9	25.28896982051028	16.72973111054816	21.80003452428753	36.18126454465403
10-11	23.62929670156184	27.223155862055293	29.450715753611746	19.69683168277112
12-13	24.658636092417012	24.783471007680888	29.320559425608362	21.23733347429374
14-15	21.84891246724411	26.170825844320007	27.36758325149646	24.61267843693943
16-17	21.045739017388748	29.227942065744106	28.025792450980173	21.70052646588697
18-19	20.868038049398994	28.331171927294164	29.916663844705365	20.884126178601477
20-21	22.099976955733176	27.61321454778783	29.649192630601583	20.63761586587741
22-23	21.588333845177587	27.549428371748203	29.551379575142718	21.310858207931492
24-25	21.292955856290238	27.68457245242242	29.84279199713416	21.17967969415318
26-27	21.298598827680905	27.905499859735123	29.618184234227584	21.17771707835639
28-29	21.098340640057835	27.98355979598116	29.707381274470485	21.210718289490522
30-31	21.67231068866142	27.79454832862605	29.513608526326497	21.01953245638603
32-33	21.496691422263392	27.9100847924932	29.389854232410322	21.20336955283309
34-35	21.40383777173083	28.131961275916407	29.503584843515302	20.960616108837463
36-37	21.526217883785232	27.870072189343254	29.40077457257786	21.202935354293654
38-39	21.223410787631448	28.024688921236258	29.528309521862578	21.22359076926972
40-41	21.519072017375947	27.892830694995634	29.441148794711484	21.14694849291693
42-43	21.580308779502676	27.97094686924766	29.112571761215932	21.336172590033733
44-45	21.600656207501093	28.32882515689392	28.750765697042326	21.319752938562658
46-47	21.690754230301206	27.99918842429455	28.49596268337251	21.81409466203173
48-49	21.71996491500755	28.105882450770125	28.518248028688713	21.655904605533607
50-51	21.432489613342938	28.38059437507538	28.374390845203834	21.812525166377853
52-53	21.58398006766494	28.37231730476101	28.32929728270301	21.71440534487104
54-55	21.769093988161885	28.20736419299265	28.233978630052224	21.789563188793238
56-57	21.860953346718716	28.40844399326653	27.9950494746399	21.735553185374847
58-59	21.572204794354775	28.508332837230533	28.32301569495943	21.59644667345526
60-61	21.79994261912055	28.558117608254296	28.048893438515442	21.593046334109708
62-63	21.682422503941932	28.534426841589912	28.057223424039464	21.72592723042869
64-65	21.557522619643862	28.550815587630574	28.071753304998897	21.81990848772667
66-67	21.77720499532544	28.616724318553487	27.7346339561299	21.871436729991174
68-69	21.75170529426204	28.457570236683143	28.01248032784177	21.77824414121304
70-71	21.733786810789088	28.036368138016275	28.065731814318358	22.164113236876275
72-73	21.841667953188743	28.072368823404048	28.142239265955872	21.94372395745134
74-75	21.653503159961506	28.39711675135702	27.987838081423938	21.96154200725754
76-77	21.781744062524194	28.36573302585672	27.77412540008695	22.078397511532135
78-79	21.89779494758125	28.330832797063604	27.724406199432277	22.04696605592287
80-81	21.70877890835963	28.29480705913871	27.828523489739965	22.167890542761693
82-83	21.890863538302664	28.232457183621683	27.9842623900869	21.89241688798875
84-85	22.20412820292491	28.155818222289607	27.737276208402044	21.902777366383436
86-87	21.72414398271493	28.362446499729604	27.893627922396387	22.01978159515908
88-89	22.022900580616454	28.342217785482227	27.808791567879876	21.826090066021443
90-91	22.02347005308324	28.294074593144742	27.92580118532045	21.75665416845157
92-93	22.006413660094392	28.337869922984925	27.834554279109536	21.821162137811143
94-95	21.75900142592929	28.455974035535046	27.877596448434556	21.907428090101106
96-97	21.81624111673383	28.5529096331884	28.087724960677857	21.54312428939991
98-99	22.819488816528334	30.44594292723725	28.337869567717828	18.396698688516587
100	22.02121496419986	31.438370287981133	22.128726968248966	24.41168777957004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1784.0
1	1708.5
2	2744.5
3	4111.5
4	6074.5
5	10503.0
6	13847.5
7	15401.0
8	15584.0
9	14373.0
10	12729.0
11	11323.5
12	12536.5
13	17506.0
14	28483.5
15	42759.0
16	54152.0
17	60534.5
18	61394.5
19	57795.0
20	51982.5
21	45955.5
22	42792.5
23	44956.5
24	51246.5
25	62784.5
26	81271.5
27	101872.5
28	117173.0
29	132632.0
30	149036.0
31	164869.5
32	186797.0
33	211597.5
34	237822.0
35	261700.5
36	288033.0
37	312383.0
38	319422.5
39	322912.5
40	333924.0
41	338270.0
42	335933.0
43	340348.5
44	345265.5
45	347442.0
46	348719.0
47	350536.0
48	352186.0
49	344408.0
50	328502.5
51	310812.5
52	292783.0
53	273460.0
54	252076.5
55	230333.5
56	208938.5
57	187924.0
58	166450.0
59	144520.0
60	119283.0
61	92147.5
62	68862.0
63	50262.5
64	36055.0
65	25340.0
66	17803.5
67	12731.0
68	9264.5
69	6560.0
70	4635.5
71	3254.0
72	2205.5
73	1424.0
74	892.0
75	573.5
76	378.0
77	238.0
78	151.0
79	98.0
80	71.5
81	61.0
82	54.5
83	40.5
84	22.0
85	11.0
86	6.0
87	3.5
88	2.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	9.203330236845344E-4
4	0.010465838359079256
5	0.010914205729592235
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.359883398161297E-5
22-23	0.0
24-25	0.005954404604094353
26-27	0.04600342513888542
28-29	0.08157286132152879
30-31	0.07394964860316706
32-33	0.034014972039574466
34-35	0.0030427958539529306
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.0462764131920627E-4
56-57	5.027066564226221E-4
58-59	6.499162875325844E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.2645457434465933E-4
72-73	0.013365575996422734
74-75	0.0287596318739171
76-77	0.002615823702789406
78-79	0.0
80-81	0.0
82-83	0.007780660283380067
84-85	0.046205515832003045
86-87	0.03930514717556988
88-89	0.009836565648752921
90-91	1.210528266969904E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	119.0
20-21	552.0
22-23	1314.0
24-25	2387.0
26-27	3888.0
28-29	7308.0
30-31	17061.0
32-33	22408.0
34-35	30360.0
36-37	43243.0
38-39	51209.0
40-41	56854.0
42-43	65187.0
44-45	79888.0
46-47	78555.0
48-49	68401.0
50-51	58800.0
52-53	54222.0
54-55	59226.0
56-57	64910.0
58-59	64112.0
60-61	65467.0
62-63	65958.0
64-65	67964.0
66-67	73157.0
68-69	69352.0
70-71	67128.0
72-73	64462.0
74-75	64344.0
76-77	64424.0
78-79	67820.0
80-81	69737.0
82-83	66104.0
84-85	67473.0
86-87	69179.0
88-89	74293.0
90-91	78927.0
92-93	81613.0
94-95	96492.0
96-97	263820.0
98-99	506240.0
100-101	5601235.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.8852242576362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.72268300626193	70.07746204436765
2	7.773522482876646	12.419791736327557
3	2.2674986659051024	5.4341891828916005
4	0.9460317565471001	3.0229583610644233
5	0.473979252086421	1.8931969423195212
6	0.2603499354314489	1.247886778644144
7	0.15569463269497008	0.8706390453983587
8	0.10256189444306658	0.655454395029791
9	0.0700859499562382	0.5038948646607202
>10	0.22009119758099302	2.6613767325397197
>50	0.004627124694631927	0.25105188074843066
>100	0.002311383506198037	0.3737599878491728
>500	2.6655079091890437E-4	0.1532656149031202
>1k	2.9616722435632373E-4	0.43507243325572936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1799141329288902E-5
2	0.0	0.0	0.0	1.1799141329288902E-5	1.1799141329288902E-5
3	0.0	0.0	0.0	3.539742398786671E-5	1.1799141329288902E-5
4	0.0	0.0	0.0	3.539742398786671E-5	1.1799141329288902E-5
5	0.0	0.0	0.0	3.539742398786671E-5	1.1799141329288902E-5
6	0.0	0.0	0.0	3.539742398786671E-5	1.1799141329288902E-5
7	0.0	0.0	0.0	3.539742398786671E-5	1.1799141329288902E-5
8	0.0	0.0	0.0	3.539742398786671E-5	4.719656531715561E-5
9	0.0	0.0	0.0	4.719656531715561E-5	5.899570664644451E-5
10-11	0.0	0.0	0.0	8.259398930502232E-5	5.899570664644451E-5
12-13	0.0	0.0	0.0	8.259398930502232E-5	5.899570664644451E-5
14-15	0.0	1.1799141329288902E-5	0.0	1.0029270129895567E-4	7.669441864037787E-5
16-17	0.0	1.1799141329288902E-5	0.0	1.1799141329288902E-4	1.1799141329288902E-4
18-19	0.0	1.1799141329288902E-5	0.0	1.4158969595146683E-4	1.2979055462217793E-4
20-21	0.0	1.1799141329288902E-5	0.0	1.5338883728075573E-4	1.4158969595146683E-4
22-23	0.0	1.1799141329288902E-5	0.0	1.5928840794540017E-4	1.7698711993933353E-4
24-25	0.0	1.1799141329288902E-5	0.0	2.182841145918447E-4	2.0058540259791134E-4
26-27	0.0	1.1799141329288902E-5	0.0	3.2447638655544485E-4	2.1238454392720024E-4
28-29	0.0	1.1799141329288902E-5	0.0	7.728437570684232E-4	2.1238454392720024E-4
30-31	0.0	1.1799141329288902E-5	0.0	0.0023362299831992026	2.1238454392720024E-4
32-33	0.0	1.1799141329288902E-5	0.0	0.005710784403375829	2.3598282658577805E-4
34-35	0.0	1.1799141329288902E-5	0.0	0.009923077857931967	2.47781967915067E-4
36-37	0.0	2.3598282658577805E-5	0.0	0.017970092244506998	2.47781967915067E-4
38-39	0.0	2.3598282658577805E-5	0.0	0.03873658098405547	2.713802505736448E-4
40-41	0.0	2.3598282658577805E-5	0.0	0.059798048256836156	2.8317939190293366E-4
42-43	0.0	2.3598282658577805E-5	0.0	0.07237003334319349	2.8317939190293366E-4
44-45	0.0	2.3598282658577805E-5	0.0	0.08937849556936343	2.8317939190293366E-4
46-47	0.0	2.3598282658577805E-5	0.0	0.10788544874435307	2.8317939190293366E-4
48-49	0.0	2.3598282658577805E-5	0.0	0.12472872299191298	2.8317939190293366E-4
50-51	0.0	2.3598282658577805E-5	0.0	0.14084045047705698	2.8317939190293366E-4
52-53	0.0	2.3598282658577805E-5	0.0	0.18634383901345963	2.890789625675781E-4
54-55	0.0	2.3598282658577805E-5	0.0	0.24482038344141543	2.949785332322226E-4
56-57	0.0	2.3598282658577805E-5	0.0	0.30569215355921686	3.00878103896867E-4
58-59	0.0	2.3598282658577805E-5	0.0	0.35136073007422963	3.0677767456151146E-4
60-61	0.0	2.3598282658577805E-5	0.0	0.38466970604681217	3.0677767456151146E-4
62-63	0.0	2.3598282658577805E-5	0.0	0.40891694147850083	3.185768158908004E-4
64-65	0.0	2.3598282658577805E-5	0.0	0.4164506932172518	3.185768158908004E-4
66-67	0.0	2.3598282658577805E-5	0.0	0.42268653940978096	3.2447638655544485E-4
68-69	0.0	2.3598282658577805E-5	0.0	0.4295418405220979	3.3037595722008926E-4
70-71	0.0	2.3598282658577805E-5	0.0	0.43487505240293645	3.3037595722008926E-4
72-73	0.0	2.3598282658577805E-5	0.0	0.43892215787888256	3.5397423987866707E-4
74-75	0.0	2.3598282658577805E-5	0.0	0.4422082187390895	3.5397423987866707E-4
76-77	0.0	2.3598282658577805E-5	0.0	0.44486302553817947	3.5397423987866707E-4
78-79	0.0	2.3598282658577805E-5	0.0	0.44689837741748184	3.5397423987866707E-4
80-81	0.0	2.3598282658577805E-5	0.0	0.44760632589723914	3.6577338120795594E-4
82-83	0.0	2.3598282658577805E-5	0.0	0.44809009069174	3.775725225372449E-4
84-85	0.0	2.3598282658577805E-5	0.0	0.4483201739476611	3.775725225372449E-4
86-87	0.0	2.3598282658577805E-5	0.0	0.44838506922497223	3.893716638665338E-4
88	0.0	2.3598282658577805E-5	0.0	0.44840276793696615	3.893716638665338E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12975	0.0	48.671658	1
GTATCAA	22820	0.0	37.42775	1
ATCAACG	28490	0.0	29.365337	3
CAACGCA	28920	0.0	28.911411	5
TCAACGC	29000	0.0	28.819336	4
AACGCAG	29225	0.0	28.767792	6
TATCAAC	29710	0.0	28.503948	2
ACGCAGA	32660	0.0	25.63772	7
CGCAGAG	33120	0.0	25.268768	8
GCAGAGT	38020	0.0	21.787872	9
TACATGG	26790	0.0	20.309835	2
GAGTACT	23925	0.0	20.011606	12-13
ACATGGG	26870	0.0	19.773285	3
GTACATG	29150	0.0	19.30918	1
AGAGTAC	35880	0.0	19.047379	10-11
CAGAGTA	38000	0.0	19.044973	10-11
CATGGGG	18215	0.0	17.791409	4
GTACTTT	27200	0.0	17.461723	14-15
AGTACTT	26565	0.0	16.947601	12-13
GTGGTAT	6620	0.0	16.747261	1
>>END_MODULE