FastQCFastQC Report
Fri 27 May 2016
SRR2138650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8061775
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114100.050.0384251
GTATCAA202000.036.196991
TCAACGC240650.030.0078814
ATCAACG240550.029.9496673
CAACGCA245350.029.3610025
TATCAAC251800.028.8504222
AACGCAG251650.028.7249896
ACGCAGA284400.025.402077
CGCAGAG288300.025.0286458
TGGTATC53600.022.4417922
GTGGTAT57800.022.0746021
GCAGAGT336200.021.2455129
GAGTACT210550.020.16480812-13
GTACATG230450.019.909431
TACATGG232200.019.2968462
CAGAGTA331300.019.28449210-11
AGAGTAC308150.018.6912910-11
ACATGGG236500.018.4192143
GTACTTT233300.018.0516814-15
AGTACTT226850.016.95506712-13
Produced by FastQC (version 0.11.5)