##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8061775
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7807067302176	33.0	33.0	33.0	33.0	33.0
2	32.77717723454202	33.0	33.0	33.0	33.0	33.0
3	32.77874041386667	33.0	33.0	33.0	33.0	33.0
4	36.72030005798971	37.0	37.0	37.0	37.0	37.0
5	36.735130414828	37.0	37.0	37.0	37.0	37.0
6	36.74370346480769	37.0	37.0	37.0	37.0	37.0
7	36.73168489569605	37.0	37.0	37.0	37.0	37.0
8	36.72481047908184	37.0	37.0	37.0	37.0	37.0
9	36.722716647388445	37.0	37.0	37.0	37.0	37.0
10-11	36.69448489941731	37.0	37.0	37.0	37.0	37.0
12-13	36.673716644287396	37.0	37.0	37.0	37.0	37.0
14-15	38.919952988020626	40.0	40.0	40.0	37.0	40.0
16-17	38.87288841477218	40.0	37.0	40.0	37.0	40.0
18-19	38.89754086413972	40.0	40.0	40.0	37.0	40.0
20-21	38.93297991143645	40.0	40.0	40.0	37.0	40.0
22-23	38.95885609539488	40.0	40.0	40.0	37.0	40.0
24-25	38.96818380694485	40.0	40.0	40.0	37.0	40.0
26-27	38.87920760415955	40.0	40.0	40.0	37.0	40.0
28-29	38.80842034430075	40.0	38.5	40.0	37.0	40.0
30-31	38.74933418339971	40.0	37.0	40.0	37.0	40.0
32-33	38.438203156932104	40.0	37.0	40.0	37.0	40.0
34-35	38.57816250332619	40.0	37.0	40.0	37.0	40.0
36-37	38.55167486009395	40.0	37.0	40.0	37.0	40.0
38-39	38.5872370922813	40.0	37.0	40.0	37.0	40.0
40-41	38.55450995272599	40.0	37.0	40.0	37.0	40.0
42-43	38.26518501489625	40.0	37.0	40.0	37.0	40.0
44-45	38.174640817685166	40.0	37.0	40.0	37.0	40.0
46-47	38.20682115767289	40.0	37.0	40.0	37.0	40.0
48-49	38.08002410750756	40.0	37.0	40.0	37.0	40.0
50-51	38.19331284255395	40.0	37.0	40.0	37.0	40.0
52-53	38.185216730720576	40.0	37.0	40.0	37.0	40.0
54-55	38.134566211424115	40.0	37.0	40.0	37.0	40.0
56-57	38.029424976701875	40.0	37.0	40.0	35.0	40.0
58-59	37.90841560026912	40.0	37.0	40.0	33.0	40.0
60-61	37.67470362382317	40.0	37.0	40.0	33.0	40.0
62-63	37.49138418919331	38.5	37.0	40.0	33.0	40.0
64-65	37.32486241953724	37.0	37.0	40.0	33.0	40.0
66-67	37.094092599429246	37.0	37.0	40.0	33.0	40.0
68-69	36.82205258649941	37.0	37.0	40.0	33.0	40.0
70-71	36.367807596657194	37.0	37.0	40.0	33.0	40.0
72-73	36.257440559384264	37.0	37.0	37.0	33.0	40.0
74-75	36.01710335606084	37.0	37.0	37.0	33.0	40.0
76-77	33.97944888373662	35.0	33.0	37.0	27.0	37.0
78-79	35.37360658948607	37.0	33.0	37.0	33.0	37.0
80-81	35.66907620985529	37.0	37.0	37.0	33.0	37.0
82-83	35.658754970726996	37.0	37.0	37.0	33.0	37.0
84-85	35.545162017859525	37.0	37.0	37.0	33.0	37.0
86-87	35.10907516704903	37.0	33.0	37.0	33.0	37.0
88-89	35.03321009428373	37.0	33.0	37.0	33.0	37.0
90-91	35.19529205825694	37.0	33.0	37.0	33.0	37.0
92-93	35.18493275535161	37.0	33.0	37.0	33.0	37.0
94-95	35.15318920320965	37.0	33.0	37.0	33.0	37.0
96-97	35.11143632066681	37.0	33.0	37.0	33.0	37.0
98-99	35.07325457012283	37.0	33.0	37.0	33.0	37.0
100	35.015638531139	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	24.0
21	139.0
22	536.0
23	1656.0
24	3854.0
25	8234.0
26	15264.0
27	25150.0
28	38021.0
29	54706.0
30	75989.0
31	103227.0
32	139759.0
33	192082.0
34	275299.0
35	425015.0
36	829887.0
37	2331667.0
38	3406489.0
39	134771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63533465024762	20.359002825060237	14.551844972106018	23.453817552586123
2	14.463080401028309	20.99249855025723	42.04407838224212	22.500342666472335
3	17.993031469530322	26.212183553508943	30.55347954536969	25.241305431591044
4	12.23981952376256	18.202978701496548	39.40569874685131	30.151503027889582
5	13.19655315335902	37.82611613686305	35.7016788744863	13.275651835291633
6	29.50255992011685	38.16026867532275	19.007290578067483	13.329880826492923
7	26.12558648684688	32.92535452800407	23.324069451206462	17.624989533942585
8	24.0833935454661	36.78839957701623	21.327747301307713	17.800459576209953
9	25.346403242462113	17.00556018990855	21.61506616098812	36.03297040664122
10-11	23.850504386440953	27.036893240012276	29.099404287517327	20.01319808602944
12-13	24.756334678157106	24.634909805843005	29.19215681410111	21.416598701898774
14-15	21.89308806063181	26.074270244456088	27.105519566100515	24.927122128811583
16-17	21.328038800388253	29.159223620108477	27.727894911480412	21.78484266802286
18-19	21.233959022671804	28.270387104576844	29.625876435400393	20.869777437350955
20-21	22.13545257195105	27.42329677657882	29.572095376653778	20.869155274816354
22-23	21.83189597155372	27.34208466025526	29.40931644648788	21.41670292170314
24-25	21.57186800860155	27.471928934199113	29.507631830708736	21.4485712264906
26-27	21.516288073748786	27.667734346915573	29.38001076579309	21.43596681354255
28-29	21.419473737944983	27.767071458935533	29.414232381587745	21.399222421531743
30-31	21.775034876491066	27.563269699339394	29.316200581063367	21.345494843106174
32-33	21.608545562621437	27.659141672009536	29.139759978772656	21.59255278659637
34-35	21.702215532682235	27.801118594104253	29.16707151094378	21.32959436226973
36-37	21.715999617186807	27.73564304813312	29.118308141179348	21.430049193500725
38-39	21.515875524628647	27.875181690052298	29.179690123103867	21.429252662215184
40-41	21.79348872243908	27.770107490608844	29.009348513616622	21.42705527333545
42-43	21.805159765686305	27.81879849663655	28.740694930309346	21.6353468073678
44-45	21.889671561410363	27.934707556797928	28.565379628594727	21.610241253196985
46-47	21.851312036553242	27.977299248522915	28.322846138399843	21.848542576524007
48-49	21.866734915205182	28.081545489201424	28.248928431868798	21.802791163724596
50-51	21.838831821654512	28.22378815381439	28.116909608612627	21.820470415918475
52-53	21.955656830624307	28.123018675559287	28.007418184907685	21.913906308908725
54-55	21.974051427937216	28.04935212094286	28.074583184631983	21.902013266487945
56-57	21.92732419454234	28.11227747238197	28.075108637914646	21.885289695161045
58-59	21.94410300983637	28.15892621548216	28.038843526351247	21.858127248330227
60-61	22.05340711767899	28.146101366213657	27.987001479849603	21.81349003625775
62-63	21.920271103574336	28.180078236835733	28.003838890479443	21.89581176911049
64-65	21.902702564762937	28.16717264040208	27.974130814233888	21.955993980601093
66-67	22.001258519492545	28.18501313154143	27.811602945699033	22.00212540326699
68-69	21.97980114475832	28.20177787886849	27.826683074009097	21.991737902364093
70-71	21.94969394351663	27.959018940867487	27.88344891200067	22.207838203615214
72-73	21.97082501111363	27.993683828893225	27.894918333029533	22.140572826963613
74-75	21.891200078949467	28.131659236092986	27.82505718084558	22.152083504111964
76-77	22.03866795683387	28.058306141970142	27.641353263226208	22.261672637969784
78-79	22.02431452389209	28.10858285383909	27.65554836616738	22.211554256101447
80-81	21.952010135280684	28.103981704677434	27.719095371529406	22.224912788512476
82-83	21.99092570796455	28.12752330287073	27.78992562517922	22.0916253639855
84-85	22.149509404239915	28.06871232599609	27.717301527241716	22.06447674252228
86-87	22.03041283248274	28.077482814949224	27.774360161005156	22.11774419156288
88-89	22.091511005087234	28.088050575579427	27.720485083659714	22.09995333567362
90-91	22.051826803243692	28.0887583222822	27.778042107210666	22.081372767263446
92-93	22.0268832825883	28.056589026623964	27.81356039042683	22.10296730036091
94-95	21.88536982403575	28.169595036800892	27.842801776259783	22.102233362903576
96-97	21.99365351476062	28.269371052252783	27.95012755589567	21.786847877090928
98-99	23.06648181336897	30.164211424331494	28.17020487340139	18.599101888898147
100	21.99030625863621	31.197223664724984	22.01664894829114	24.795821128347665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1911.0
1	1711.0
2	2288.5
3	3312.5
4	4721.0
5	7726.0
6	10164.5
7	11695.0
8	12548.0
9	12350.0
10	11407.0
11	10605.0
12	11786.5
13	15706.0
14	24379.0
15	35576.0
16	44610.0
17	50307.5
18	51757.0
19	49077.0
20	44653.0
21	40408.0
22	38185.5
23	40154.5
24	46770.5
25	57396.5
26	71553.0
27	87456.0
28	103857.5
29	122078.5
30	139621.5
31	156586.5
32	175568.5
33	194038.0
34	212830.0
35	230935.0
36	250427.0
37	269230.5
38	281269.0
39	291279.0
40	302759.5
41	311301.0
42	316852.0
43	323182.0
44	328853.0
45	333770.5
46	335735.5
47	335066.0
48	332046.5
49	324721.5
50	314656.0
51	301334.5
52	285045.5
53	266998.0
54	249548.5
55	231049.5
56	209808.0
57	187278.0
58	164358.0
59	141301.0
60	117193.0
61	94770.0
62	75185.5
63	58330.0
64	43854.5
65	32289.0
66	23988.5
67	18009.0
68	13442.5
69	9593.0
70	6636.5
71	4596.0
72	3141.0
73	2105.5
74	1412.5
75	892.5
76	573.5
77	377.5
78	251.5
79	168.5
80	117.5
81	87.5
82	67.5
83	55.0
84	48.0
85	42.0
86	33.0
87	23.5
88	15.5
89	8.0
90	4.0
91	3.5
92	2.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012900384840807391
4	0.010915710249913946
5	0.0118832391129745
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.2404523160516204E-5
22-23	0.0
24-25	0.005577297087974696
26-27	0.04495521668550971
28-29	0.0808466414774005
30-31	0.07285349312329781
32-33	0.03332015682022817
34-35	0.002922286656839125
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.4032086704931945E-4
56-57	3.634093368068985E-4
58-59	1.3558705227714726E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.9913011431917628E-4
72-73	0.012688407429137861
74-75	0.0285560387306103
76-77	0.002429217667167562
78-79	0.0
80-81	0.0
82-83	0.00734815781683532
84-85	0.04538767949245708
86-87	0.03811842562193764
88-89	0.009645242576693374
90-91	9.35985312518476E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	121.0
20-21	478.0
22-23	1077.0
24-25	2476.0
26-27	3366.0
28-29	6535.0
30-31	15214.0
32-33	19056.0
34-35	27008.0
36-37	38322.0
38-39	45052.0
40-41	48999.0
42-43	57484.0
44-45	70003.0
46-47	68248.0
48-49	60705.0
50-51	52558.0
52-53	49208.0
54-55	52644.0
56-57	54353.0
58-59	55680.0
60-61	54578.0
62-63	54184.0
64-65	57364.0
66-67	60298.0
68-69	60939.0
70-71	60385.0
72-73	58264.0
74-75	58635.0
76-77	59206.0
78-79	62083.0
80-81	63526.0
82-83	61085.0
84-85	61565.0
86-87	64308.0
88-89	68431.0
90-91	71944.0
92-93	76612.0
94-95	92284.0
96-97	261624.0
98-99	497422.0
100-101	5428451.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.37873518651486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81448994011649	76.68247552509109
2	7.112990526307342	12.14596269059567
3	1.7405156333151526	4.458090700344108
4	0.638574414709717	2.180827034015386
5	0.28063970626556284	1.1980331582034403
6	0.14705160001164794	0.7533047769688677
7	0.07832970321674282	0.46813836917264107
8	0.05022788008984329	0.34307142985366024
9	0.03433038857902458	0.2637976639804825
>10	0.10069768097922842	1.2847920910290545
>50	0.0016507295791578869	0.09160871701903546
>100	4.564971770982522E-4	0.08507204597970325
>500	2.2649826556330083E-5	0.01840990493481875
>1k	2.2649826390800204E-5	0.026415892812159506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.721264857925209E-5	0.0	0.0	0.0
3	0.0	3.721264857925209E-5	0.0	0.0	0.0
4	0.0	6.202108096542014E-5	0.0	0.0	0.0
5	0.0	6.202108096542014E-5	0.0	0.0	0.0
6	0.0	6.202108096542014E-5	0.0	0.0	0.0
7	0.0	6.202108096542014E-5	0.0	0.0	0.0
8	0.0	7.442529715850418E-5	0.0	0.0	0.0
9	0.0	1.1163794573775626E-4	0.0	0.0	0.0
10-11	0.0	1.1163794573775626E-4	0.0	6.202108096542015E-6	0.0
12-13	0.0	1.1163794573775626E-4	0.0	1.240421619308403E-5	0.0
14-15	0.0	1.6125481051009236E-4	0.0	1.240421619308403E-5	0.0
16-17	0.0	1.736590267031764E-4	0.0	1.8606324289626044E-5	0.0
18-19	0.0	1.8606324289626043E-4	0.0	2.480843238616806E-5	0.0
20-21	0.0	1.9846745908934447E-4	0.0	2.480843238616806E-5	0.0
22-23	1.240421619308403E-5	1.9846745908934447E-4	0.0	4.34147566757941E-5	6.202108096542015E-6
24-25	1.240421619308403E-5	2.046695671858865E-4	0.0	8.68295133515882E-5	1.240421619308403E-5
26-27	1.240421619308403E-5	2.108716752824285E-4	0.0	1.4264848622046633E-4	1.240421619308403E-5
28-29	1.240421619308403E-5	2.108716752824285E-4	0.0	4.4655178295102503E-4	1.240421619308403E-5
30-31	1.240421619308403E-5	2.3568010766859655E-4	0.0	0.0011846026464395248	1.240421619308403E-5
32-33	1.240421619308403E-5	2.3568010766859655E-4	0.0	0.0030328308592090453	2.480843238616806E-5
34-35	1.240421619308403E-5	3.039032967305587E-4	0.0	0.0056191099354670655	2.480843238616806E-5
36-37	1.240421619308403E-5	3.7212648579252087E-4	0.0	0.009824139224922552	3.721264857925209E-5
38-39	1.240421619308403E-5	3.9693491817868894E-4	0.0	0.02182521839173135	3.721264857925209E-5
40-41	1.240421619308403E-5	4.27945458661399E-4	0.0	0.03432866831436005	3.721264857925209E-5
42-43	1.240421619308403E-5	4.7756232343373515E-4	0.0	0.04185182543546552	4.34147566757941E-5
44-45	1.240421619308403E-5	5.829981610749493E-4	0.0	0.05353039498125413	4.961686477233612E-5
46-47	1.240421619308403E-5	6.450192420403696E-4	0.0	0.0650104970679534	4.961686477233612E-5
48-49	1.240421619308403E-5	6.512213501369115E-4	0.0	0.0752253691029581	6.202108096542014E-5
50-51	1.240421619308403E-5	7.132424311023317E-4	0.0	0.08610386670429279	6.202108096542014E-5
52-53	1.240421619308403E-5	7.194445391988737E-4	0.0	0.11730667253799566	6.822318906196216E-5
54-55	1.240421619308403E-5	7.256466472954158E-4	0.0	0.15628692192476223	7.442529715850418E-5
56-57	1.240421619308403E-5	7.566571877781258E-4	0.0	0.1986039054674684	7.442529715850418E-5
58-59	1.240421619308403E-5	7.752635120677518E-4	0.0	0.2377392075566485	7.442529715850418E-5
60-61	1.240421619308403E-5	8.062740525504619E-4	0.0	0.25219632152968796	7.442529715850418E-5
62-63	1.240421619308403E-5	8.310824849366299E-4	0.0	0.2728431393830763	7.442529715850418E-5
64-65	1.240421619308403E-5	9.365183225778442E-4	0.0	0.2792561191549008	7.442529715850418E-5
66-67	1.240421619308403E-5	9.551246468674703E-4	0.0	0.2847139742798577	7.442529715850418E-5
68-69	1.240421619308403E-5	9.613267549640123E-4	0.0	0.2898245113514083	7.442529715850418E-5
70-71	1.240421619308403E-5	9.675288630605543E-4	0.0	0.29458152826145606	7.442529715850418E-5
72-73	1.240421619308403E-5	9.675288630605543E-4	0.0	0.2982779846869951	7.442529715850418E-5
74-75	1.240421619308403E-5	0.0010109436197363484	0.0	0.301286007113818	7.442529715850418E-5
76-77	1.240421619308403E-5	0.0010481562683156004	0.0	0.30410176418964807	7.442529715850418E-5
78-79	1.240421619308403E-5	0.0010605604845086846	0.0	0.3063221188882101	7.442529715850418E-5
80-81	1.240421619308403E-5	0.0010667625926052266	0.0	0.30693612758976774	8.062740525504618E-5
82-83	1.240421619308403E-5	0.0010667625926052266	0.0	0.3073950835889119	1.1163794573775626E-4
84-85	1.240421619308403E-5	0.0010791668087983106	0.0	0.3075749447237116	1.2404216193084028E-4
86-87	1.240421619308403E-5	0.0011039752411844785	0.0	0.3076617742370632	1.2404216193084028E-4
88	1.240421619308403E-5	0.0011411878897637307	0.0	0.3076741784532563	1.2404216193084028E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11410	0.0	50.038425	1
GTATCAA	20200	0.0	36.19699	1
TCAACGC	24065	0.0	30.007881	4
ATCAACG	24055	0.0	29.949667	3
CAACGCA	24535	0.0	29.361002	5
TATCAAC	25180	0.0	28.850422	2
AACGCAG	25165	0.0	28.724989	6
ACGCAGA	28440	0.0	25.40207	7
CGCAGAG	28830	0.0	25.028645	8
TGGTATC	5360	0.0	22.441792	2
GTGGTAT	5780	0.0	22.074602	1
GCAGAGT	33620	0.0	21.245512	9
GAGTACT	21055	0.0	20.164808	12-13
GTACATG	23045	0.0	19.90943	1
TACATGG	23220	0.0	19.296846	2
CAGAGTA	33130	0.0	19.284492	10-11
AGAGTAC	30815	0.0	18.69129	10-11
ACATGGG	23650	0.0	18.419214	3
GTACTTT	23330	0.0	18.05168	14-15
AGTACTT	22685	0.0	16.955067	12-13
>>END_MODULE