FastQCFastQC Report
Fri 27 May 2016
SRR2138649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8100872
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138000.051.2580831
GTATCAA252600.038.253661
CTTATAC61550.030.9971491
ATCAACG307650.030.8826433
TCAACGC311150.030.5350674
CAACGCA313850.030.282775
TATCAAC316500.030.2478432
AACGCAG321050.029.653036
ACGCAGA365000.026.0708167
CGCAGAG367050.025.9252078
TATACAC93000.025.7808533
GTGGTAT62900.024.6446441
TGGTATC60850.024.0052012
GCAGAGT410300.023.099029
GAGTACT261700.020.74323312-13
CAGAGTA410400.019.86695910-11
TACATGG271600.019.61552
GTACTTT286450.018.90695614-15
AGAGTAC388700.018.90037210-11
ACATGGG277450.018.7260823
Produced by FastQC (version 0.11.5)