##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8100872
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76197969798807	33.0	33.0	33.0	33.0	33.0
2	32.76276516898428	33.0	33.0	33.0	33.0	33.0
3	32.75984511297055	33.0	33.0	33.0	33.0	33.0
4	36.70170816178801	37.0	37.0	37.0	37.0	37.0
5	36.71946550939207	37.0	37.0	37.0	37.0	37.0
6	36.72774474155375	37.0	37.0	37.0	37.0	37.0
7	36.714357046994444	37.0	37.0	37.0	37.0	37.0
8	36.706385806367514	37.0	37.0	37.0	37.0	37.0
9	36.70465562225894	37.0	37.0	37.0	37.0	37.0
10-11	36.676079377627495	37.0	37.0	37.0	37.0	37.0
12-13	36.65369320734854	37.0	37.0	37.0	37.0	37.0
14-15	38.85340374468329	40.0	37.0	40.0	37.0	40.0
16-17	38.800077942226466	40.0	37.0	40.0	37.0	40.0
18-19	38.83599031067273	40.0	38.5	40.0	37.0	40.0
20-21	38.87658683835919	40.0	40.0	40.0	37.0	40.0
22-23	38.904872808811646	40.0	40.0	40.0	37.0	40.0
24-25	38.91406289158529	40.0	40.0	40.0	37.0	40.0
26-27	38.81658065280487	40.0	37.0	40.0	37.0	40.0
28-29	38.73920665108589	40.0	37.0	40.0	37.0	40.0
30-31	38.67024894161561	40.0	37.0	40.0	37.0	40.0
32-33	38.35308117293762	40.0	37.0	40.0	37.0	40.0
34-35	38.48229136536713	40.0	37.0	40.0	37.0	40.0
36-37	38.45905629516482	40.0	37.0	40.0	37.0	40.0
38-39	38.476102786598375	40.0	37.0	40.0	37.0	40.0
40-41	38.45846332858765	40.0	37.0	40.0	37.0	40.0
42-43	38.170175711307465	40.0	37.0	40.0	37.0	40.0
44-45	38.07350745082617	40.0	37.0	40.0	35.0	40.0
46-47	38.10515610099097	40.0	37.0	40.0	35.0	40.0
48-49	37.97583237970327	40.0	37.0	40.0	33.0	40.0
50-51	38.07552091549721	40.0	37.0	40.0	37.0	40.0
52-53	38.07780980812241	40.0	37.0	40.0	37.0	40.0
54-55	38.02646162371265	40.0	37.0	40.0	37.0	40.0
56-57	37.9224684085735	40.0	37.0	40.0	33.0	40.0
58-59	37.80281462314883	40.0	37.0	40.0	33.0	40.0
60-61	37.57543882726088	40.0	37.0	40.0	33.0	40.0
62-63	37.393269410781	37.0	37.0	40.0	33.0	40.0
64-65	37.22670390245659	37.0	37.0	40.0	33.0	40.0
66-67	36.996121479267266	37.0	37.0	40.0	33.0	40.0
68-69	36.71749955641063	37.0	37.0	40.0	33.0	40.0
70-71	36.27803592834158	37.0	37.0	40.0	33.0	40.0
72-73	36.1639493098728	37.0	37.0	37.0	33.0	40.0
74-75	35.930847691946525	37.0	37.0	37.0	33.0	40.0
76-77	33.89165301015998	35.0	33.0	37.0	27.0	37.0
78-79	35.30990299651417	37.0	33.0	37.0	33.0	37.0
80-81	35.61278737370182	37.0	37.0	37.0	33.0	37.0
82-83	35.59937104144285	37.0	37.0	37.0	33.0	37.0
84-85	35.485253764495006	37.0	35.0	37.0	33.0	37.0
86-87	35.05617867026598	37.0	33.0	37.0	33.0	37.0
88-89	34.97123049736028	37.0	33.0	37.0	33.0	37.0
90-91	35.120579830015444	37.0	33.0	37.0	33.0	37.0
92-93	35.10472955856304	37.0	33.0	37.0	33.0	37.0
94-95	35.067138563039435	37.0	33.0	37.0	33.0	37.0
96-97	35.02367145107172	37.0	33.0	37.0	33.0	37.0
98-99	34.990574285662824	37.0	33.0	37.0	33.0	37.0
100	34.92880567527394	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	27.0
21	135.0
22	584.0
23	1714.0
24	4231.0
25	9067.0
26	16604.0
27	27306.0
28	41886.0
29	60366.0
30	81136.0
31	110365.0
32	148416.0
33	203360.0
34	290111.0
35	446574.0
36	870496.0
37	2411971.0
38	3251152.0
39	125367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38849496696158	20.612126694508937	14.870954633032099	23.128423705497383
2	14.394919460522274	20.749716820608942	42.65962972875019	22.195733990118594
3	17.974740735162868	26.489613975920868	30.871544627128962	24.66410066178731
4	11.97891454447295	18.2974400835754	39.70795770019991	30.015687671751735
5	12.80521017798536	38.16525991630609	35.837084777367686	13.192445128340868
6	28.948402097946985	38.70685773087144	18.762399899665123	13.582340271516449
7	25.752869567621854	33.474507435742716	22.968206385682922	17.8044166109525
8	24.13450058215955	36.735810663345866	21.11764017503301	18.01204857946157
9	25.157254675792927	17.272313400335175	21.54500157513907	36.025430348732826
10-11	23.87694806188766	27.34948163605103	28.730493457988228	20.04307684407308
12-13	24.9054484504878	24.697693038477833	28.84966581375437	21.547192697279996
14-15	21.97403563468229	26.17437974578539	27.134838076691004	24.716746542841314
16-17	21.09190837727099	29.33650846476774	27.741772737552207	21.829810420409064
18-19	20.894324709734953	28.15469742022834	29.974254623452882	20.976723246583827
20-21	22.252452101391178	27.3885174191629	29.781037576359093	20.57799290308683
22-23	21.635862699354192	27.37372274614625	29.661079199954866	21.329335354544696
24-25	21.304831604717286	27.47070218683081	30.089242473747234	21.13522373470467
26-27	21.359229899306236	27.736431648645386	29.80746370536447	21.09687474668391
28-29	21.07702489290366	27.83913676335979	29.993684973688005	21.090153370048544
30-31	21.656522136123968	27.61525997390743	29.75868243363496	20.969535456333638
32-33	21.51758590686858	27.629415884883407	29.561353119989842	21.29164508825817
34-35	21.284785938424662	27.983043409071694	29.826834902305965	20.905335750197676
36-37	21.54291567481498	27.65739229821535	29.645512108136803	21.15417991883287
38-39	21.180592031155967	27.868325122008926	29.73723134347559	21.21385150335952
40-41	21.54728827247761	27.81244926339047	29.563809928086076	21.076452536045846
42-43	21.67116060681707	27.779317275142667	29.28097487601781	21.268547242022446
44-45	21.65076738566182	28.229092094171794	28.88270246646913	21.237438053697268
46-47	21.739109579286847	27.806251181770325	28.523017369406638	21.931621869536187
48-49	21.743767888584614	27.919030511938903	28.664613333635288	21.6725882658412
50-51	21.453413075388898	28.276158291812653	28.501611734719706	21.76881689807874
52-53	21.671109144114002	28.28419124557883	28.41379185613993	21.630907754167236
54-55	21.799746641940505	28.116587851528614	28.3022953296914	21.781370176839477
56-57	21.91533350187201	28.44650894563385	27.962262019629307	21.67589553286484
58-59	21.621323229495985	28.638425181846376	28.381304002654627	21.35894758600301
60-61	21.87576337540273	28.699951879633495	27.911950798558134	21.51233394640564
62-63	21.684338642116934	28.6897881929497	27.923700461576857	21.702172703356506
64-65	21.590903443372646	28.69837228200059	28.016553320663295	21.694170953963464
66-67	21.885312370730887	28.65856865319219	27.717941895733066	21.738177080343856
68-69	21.928180129917802	28.41473981816305	27.998340083555505	21.658739968363644
70-71	21.8459685937955	27.93997482212742	28.083327605850016	22.13072897822706
72-73	21.88415037730379	28.10819300377627	28.179344687901747	21.828311931018188
74-75	21.67282139186154	28.35423359394238	28.01659792772224	21.956347086473833
76-77	21.71142285606337	28.371716114187524	27.86326302990511	22.05359799984399
78-79	21.934968458411873	28.426426457786786	27.739262172248324	21.899342911553017
80-81	21.759906992469116	28.267497550006045	27.906219916276658	22.066375541248185
82-83	21.98537387134706	28.214791479663454	28.03161893297962	21.768215716009866
84-85	22.287888550079803	28.126196942489806	27.796007763594467	21.789906743835925
86-87	21.712565595496653	28.347513808344573	27.908814443170822	22.03110615298796
88-89	21.999596657027077	28.26785479371245	27.90660692849944	21.825941620761036
90-91	21.984967100303134	28.308817770630405	28.016259112939935	21.689956016126523
92-93	21.97463640955582	28.306441808360976	27.900981966925638	21.817939815157565
94-95	21.76737108688462	28.460638615241933	27.859719637078207	21.912270660795237
96-97	21.730301825656976	28.602294897999354	28.136033103943664	21.531370172400006
98-99	22.808987791930782	30.492576429652395	28.270959537240227	18.427476241176596
100	21.94500257695382	31.682732168600005	22.037436789937182	24.334828464509
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2779.0
1	2343.0
2	2716.0
3	3781.0
4	5388.5
5	9035.0
6	11935.5
7	13586.5
8	14373.0
9	13714.5
10	12565.5
11	11757.0
12	13431.0
13	18937.5
14	30732.5
15	46207.0
16	58609.5
17	65808.0
18	67330.0
19	63699.0
20	57082.5
21	49709.0
22	44920.0
23	45457.5
24	50352.0
25	60667.5
26	78444.5
27	98641.0
28	112298.0
29	125767.5
30	138795.5
31	150079.5
32	168793.0
33	190999.0
34	215589.0
35	238803.5
36	264107.0
37	286377.5
38	290502.0
39	293050.0
40	306526.5
41	315392.0
42	317386.0
43	322368.5
44	326853.5
45	331054.0
46	338124.0
47	346405.0
48	344889.5
49	337002.0
50	326855.5
51	306828.0
52	284109.0
53	263399.0
54	242594.5
55	220509.0
56	200240.0
57	181528.5
58	161946.0
59	143018.0
60	119848.0
61	92265.5
62	67441.0
63	48228.0
64	34295.5
65	23794.5
66	16599.5
67	12057.5
68	8944.5
69	6564.5
70	4834.0
71	3527.5
72	2495.0
73	1649.0
74	1079.5
75	665.5
76	419.5
77	266.5
78	178.5
79	126.5
80	92.0
81	66.0
82	52.0
83	37.0
84	25.0
85	18.0
86	13.5
87	11.5
88	7.5
89	3.0
90	1.0
91	0.0
92	0.5
93	2.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0010122367073569364
4	0.010875372429042206
5	0.010863028078952489
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.8516903433052415E-5
22-23	0.0
24-25	0.0056304345732389885
26-27	0.04651437388021524
28-29	0.08256141316074155
30-31	0.07512601933543939
32-33	0.03422230539325805
34-35	0.002890456365763943
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.4738667020216024E-4
56-57	3.6482049480468596E-4
58-59	1.3630255732902599E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.8010921390469067E-4
72-73	0.012099409621479599
74-75	0.02786462366158011
76-77	0.0024768433629470147
78-79	0.0
80-81	0.0
82-83	0.0073668073252977605
84-85	0.044760476974517065
86-87	0.03761344547448517
88-89	0.009617012832178196
90-91	6.328838539462287E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	463.0
22-23	1089.0
24-25	2064.0
26-27	3585.0
28-29	6856.0
30-31	15719.0
32-33	20871.0
34-35	29008.0
36-37	40984.0
38-39	49832.0
40-41	53825.0
42-43	64731.0
44-45	78295.0
46-47	77166.0
48-49	65749.0
50-51	57355.0
52-53	55267.0
54-55	60516.0
56-57	64876.0
58-59	63678.0
60-61	65091.0
62-63	64722.0
64-65	66429.0
66-67	72392.0
68-69	64462.0
70-71	60790.0
72-73	58217.0
74-75	59691.0
76-77	58501.0
78-79	62146.0
80-81	64484.0
82-83	60176.0
84-85	60829.0
86-87	64237.0
88-89	68324.0
90-91	72811.0
92-93	76423.0
94-95	92138.0
96-97	252184.0
98-99	489651.0
100-101	5355160.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.52510259701198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.84733871851218	73.32135743216692
2	7.448524510556139	12.293804988600074
3	2.0027704906153234	4.958365206488928
4	0.7650435081586541	2.525411760078836
5	0.3640670016272526	1.5022333330737774
6	0.1991094410679772	0.9858916231301118
7	0.10939884213027966	0.6319705469556952
8	0.07237571338229987	0.47782505379249834
9	0.04643169128996725	0.34486020787116006
>10	0.13930733716584673	1.7136750441804973
>50	0.0027917400151876706	0.16145898260200117
>100	0.0022110229605516057	0.35544666779976963
>500	3.674900499850542E-4	0.2151494158936192
>1k	2.3624322154797806E-4	0.3773991599845257
>5k	2.624924683864412E-5	0.13515057738160755
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2344350089718736E-5	0.0
5	0.0	0.0	0.0	1.2344350089718736E-5	0.0
6	0.0	0.0	0.0	1.2344350089718736E-5	0.0
7	0.0	0.0	0.0	1.2344350089718736E-5	0.0
8	0.0	0.0	0.0	1.2344350089718736E-5	0.0
9	0.0	0.0	0.0	2.4688700179437472E-5	0.0
10-11	0.0	0.0	0.0	2.4688700179437472E-5	0.0
12-13	0.0	1.2344350089718736E-5	0.0	2.4688700179437472E-5	0.0
14-15	0.0	4.9377400358874943E-5	0.0	2.4688700179437472E-5	0.0
16-17	0.0	7.406610053831242E-5	0.0	2.4688700179437472E-5	0.0
18-19	0.0	7.406610053831242E-5	0.0	7.406610053831242E-5	0.0
20-21	0.0	8.023827558317179E-5	0.0	1.2344350089718735E-4	0.0
22-23	0.0	8.641045062803115E-5	0.0	1.2344350089718735E-4	0.0
24-25	0.0	9.258262567289052E-5	0.0	1.543043761214842E-4	0.0
26-27	0.0	9.875480071774989E-5	0.0	1.8516525134578104E-4	0.0
28-29	0.0	9.875480071774989E-5	0.0	3.2712527737754655E-4	0.0
30-31	0.0	9.875480071774989E-5	0.0	6.974557800691086E-4	0.0
32-33	0.0	9.875480071774989E-5	0.0	0.001456633310586811	0.0
34-35	0.0	1.1109915080746862E-4	0.0	0.0027774787701867155	0.0
36-37	0.0	1.543043761214842E-4	0.0	0.004709369559227699	0.0
38-39	0.0	1.7899307630092168E-4	0.0	0.00771521880607421	0.0
40-41	0.0	1.8516525134578104E-4	0.0	0.010609968902113255	0.0
42-43	0.0	1.9750960143549977E-4	0.0	0.01387504950084386	0.0
44-45	0.0	2.098539515252185E-4	1.2344350089718736E-5	0.01731295100083053	0.0
46-47	0.0	2.2837047665979663E-4	1.2344350089718736E-5	0.021294003904764817	0.0
48-49	0.0	2.4071482674951536E-4	1.2344350089718736E-5	0.02561452643616638	0.0
50-51	0.0	2.468870017943747E-4	1.2344350089718736E-5	0.029694334140818422	0.0
52-53	0.0	2.592313518840935E-4	1.2344350089718736E-5	0.033663042694663	0.0
54-55	0.0	2.592313518840935E-4	1.2344350089718736E-5	0.037878638250301945	0.0
56-57	0.0	2.592313518840935E-4	1.2344350089718736E-5	0.042316432107555826	0.0
58-59	0.0	2.592313518840935E-4	1.2344350089718736E-5	0.04705049036696296	0.0
60-61	0.0	2.6540352692895283E-4	1.2344350089718736E-5	0.051531489449530864	0.0
62-63	0.0	2.715757019738122E-4	1.2344350089718736E-5	0.055710051954900655	0.0
64-65	0.0	2.715757019738122E-4	1.2344350089718736E-5	0.060221911912692855	0.0
66-67	0.0	2.715757019738122E-4	1.2344350089718736E-5	0.06460415619454302	0.0
68-69	0.0	2.715757019738122E-4	1.2344350089718736E-5	0.06922711530314267	0.0
70-71	0.0	2.777478770186716E-4	1.2344350089718736E-5	0.07384390223669748	0.0
72-73	0.0	2.8392005206353096E-4	1.2344350089718736E-5	0.07778174991531775	0.0
74-75	0.0	2.8392005206353096E-4	1.2344350089718736E-5	0.08023210340812693	0.0
76-77	0.0	2.962644021532497E-4	1.2344350089718736E-5	0.08278121170165384	0.0
78-79	0.0	3.086087522429684E-4	1.2344350089718736E-5	0.08478099641618828	0.0
80-81	0.0	3.086087522429684E-4	1.2344350089718736E-5	0.08542907479589851	0.0
82-83	0.0	3.086087522429684E-4	1.2344350089718736E-5	0.08584878269894895	0.0
84-85	0.0	3.1478092728782776E-4	1.2344350089718736E-5	0.08602160360020503	0.0
86-87	0.0	3.2095310233268715E-4	1.2344350089718736E-5	0.08616356362623678	0.0
88	0.0	3.2095310233268715E-4	1.2344350089718736E-5	0.08618825232641622	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13800	0.0	51.258083	1
GTATCAA	25260	0.0	38.25366	1
CTTATAC	6155	0.0	30.997149	1
ATCAACG	30765	0.0	30.882643	3
TCAACGC	31115	0.0	30.535067	4
CAACGCA	31385	0.0	30.28277	5
TATCAAC	31650	0.0	30.247843	2
AACGCAG	32105	0.0	29.65303	6
ACGCAGA	36500	0.0	26.070816	7
CGCAGAG	36705	0.0	25.925207	8
TATACAC	9300	0.0	25.780853	3
GTGGTAT	6290	0.0	24.644644	1
TGGTATC	6085	0.0	24.005201	2
GCAGAGT	41030	0.0	23.09902	9
GAGTACT	26170	0.0	20.743233	12-13
CAGAGTA	41040	0.0	19.866959	10-11
TACATGG	27160	0.0	19.6155	2
GTACTTT	28645	0.0	18.906956	14-15
AGAGTAC	38870	0.0	18.900372	10-11
ACATGGG	27745	0.0	18.726082	3
>>END_MODULE