FastQCFastQC Report
Fri 27 May 2016
SRR2138648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7552195
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112800.051.3448141
GTATCAA207150.038.319471
ATCAACG251200.031.2587623
TCAACGC251600.031.2258874
CAACGCA256100.030.6904815
TATCAAC260950.030.2714062
AACGCAG261800.030.0837366
ACGCAGA299700.026.2364857
CGCAGAG302400.026.0163928
GTGGTAT50950.025.8961681
TGGTATC51650.023.2229142
GCAGAGT347400.022.5600999
GAGTACT213650.020.5366112-13
GTACATG237250.020.024161
CAGAGTA338150.019.94127810-11
TACATGG234500.019.8570922
AGAGTAC317100.019.3066810-11
ACATGGG242600.018.7879683
GTACTTT237100.018.523914-15
AGTACTT227250.017.82817812-13
Produced by FastQC (version 0.11.5)