##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7552195
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78417082715687	33.0	33.0	33.0	33.0	33.0
2	32.78169538789716	33.0	33.0	33.0	33.0	33.0
3	32.781112246174786	33.0	33.0	33.0	33.0	33.0
4	36.72483562725804	37.0	37.0	37.0	37.0	37.0
5	36.74051967672975	37.0	37.0	37.0	37.0	37.0
6	36.74869796132118	37.0	37.0	37.0	37.0	37.0
7	36.737527434077116	37.0	37.0	37.0	37.0	37.0
8	36.73006695404449	37.0	37.0	37.0	37.0	37.0
9	36.727321394640896	37.0	37.0	37.0	37.0	37.0
10-11	36.70107412480743	37.0	37.0	37.0	37.0	37.0
12-13	36.67950986435069	37.0	37.0	37.0	37.0	37.0
14-15	38.930868906324584	40.0	40.0	40.0	37.0	40.0
16-17	38.88746913976665	40.0	40.0	40.0	37.0	40.0
18-19	38.91446725091183	40.0	40.0	40.0	37.0	40.0
20-21	38.94656235266783	40.0	40.0	40.0	37.0	40.0
22-23	38.97455259932542	40.0	40.0	40.0	37.0	40.0
24-25	38.982579902159145	40.0	40.0	40.0	37.0	40.0
26-27	38.89121709806837	40.0	40.0	40.0	37.0	40.0
28-29	38.81884476635257	40.0	38.5	40.0	37.0	40.0
30-31	38.75856307221672	40.0	37.0	40.0	37.0	40.0
32-33	38.44274350658971	40.0	37.0	40.0	37.0	40.0
34-35	38.5859112572755	40.0	37.0	40.0	37.0	40.0
36-37	38.55790671347722	40.0	37.0	40.0	37.0	40.0
38-39	38.59368187467817	40.0	37.0	40.0	37.0	40.0
40-41	38.560590170921856	40.0	37.0	40.0	37.0	40.0
42-43	38.26795480502671	40.0	37.0	40.0	37.0	40.0
44-45	38.176919954811595	40.0	37.0	40.0	37.0	40.0
46-47	38.20317258528563	40.0	37.0	40.0	37.0	40.0
48-49	38.07407433363941	40.0	37.0	40.0	37.0	40.0
50-51	38.18222969421306	40.0	37.0	40.0	37.0	40.0
52-53	38.1733737068691	40.0	37.0	40.0	37.0	40.0
54-55	38.118881370175416	40.0	37.0	40.0	37.0	40.0
56-57	38.015650146812064	40.0	37.0	40.0	35.0	40.0
58-59	37.8942133745818	40.0	37.0	40.0	33.0	40.0
60-61	37.66091505112841	40.0	37.0	40.0	33.0	40.0
62-63	37.47793056619285	38.5	37.0	40.0	33.0	40.0
64-65	37.30928989103592	37.0	37.0	40.0	33.0	40.0
66-67	37.07757359859386	37.0	37.0	40.0	33.0	40.0
68-69	36.805998243332795	37.0	37.0	40.0	33.0	40.0
70-71	36.36215212238634	37.0	37.0	40.0	33.0	40.0
72-73	36.25178674404003	37.0	37.0	37.0	33.0	40.0
74-75	36.01665957892035	37.0	37.0	37.0	33.0	40.0
76-77	33.980191904173765	35.0	33.0	37.0	27.0	37.0
78-79	35.37967045458909	37.0	33.0	37.0	33.0	37.0
80-81	35.674088931636476	37.0	37.0	37.0	33.0	37.0
82-83	35.66900204005456	37.0	37.0	37.0	33.0	37.0
84-85	35.56314561226989	37.0	37.0	37.0	33.0	37.0
86-87	35.13373386697103	37.0	33.0	37.0	33.0	37.0
88-89	35.06284009570952	37.0	33.0	37.0	33.0	37.0
90-91	35.22589651537064	37.0	33.0	37.0	33.0	37.0
92-93	35.21639390211051	37.0	33.0	37.0	33.0	37.0
94-95	35.18760960732821	37.0	33.0	37.0	33.0	37.0
96-97	35.14478628442021	37.0	33.0	37.0	33.0	37.0
98-99	35.10779815591859	37.0	33.0	37.0	33.0	37.0
100	35.051988712059455	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	141.0
22	493.0
23	1454.0
24	3547.0
25	7492.0
26	13824.0
27	22613.0
28	35208.0
29	50742.0
30	70450.0
31	96170.0
32	130382.0
33	179340.0
34	255503.0
35	397503.0
36	778395.0
37	2199961.0
38	3184447.0
39	124507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7902874594737	20.10097726554995	14.663657387024568	23.445077887951783
2	14.583455538422935	20.89936766728084	41.805541302892735	22.71163549140349
3	18.17042219895571	25.951685245818286	30.549649547947112	25.3282430072789
4	12.406197198989796	18.1706522028454	39.05983925781718	30.36331134034762
5	13.403975898875817	37.614007775299484	35.40935533505338	13.572660990771318
6	29.76292322960411	37.80528707216909	18.86091394621034	13.570875752016468
7	26.35251076011676	33.00488136230592	22.886048360774584	17.75655951680273
8	24.377786855344706	36.42507906641711	21.179445181169182	18.017688897068997
9	25.604529544059705	17.01007455448383	21.38008883510026	36.00530706635621
10-11	24.096590461448624	26.90833592088128	28.754607104292194	20.2404665133779
12-13	25.012549331684365	24.548876187651405	28.916533537600657	21.522040943063573
14-15	22.144634771745167	25.92276814886268	26.912434067181795	25.020163012210357
16-17	21.44722825615599	28.9797270859664	27.59425570976385	21.978788948113763
18-19	21.428127848923392	28.0630002270863	29.53341379559188	20.975458128398433
20-21	22.3422863106437	27.168438055376225	29.514714020988926	20.974561612991153
22-23	22.044299113167572	27.114376076858015	29.353426340230126	21.487898469744287
24-25	21.719883091400757	27.243881296087764	29.458464909373056	21.577770703138427
26-27	21.687784983277623	27.435998516983446	29.303899493152446	21.572317006586488
28-29	21.59041050944948	27.559542928741127	29.33344902473607	21.516597537073324
30-31	21.946873577905244	27.336531774473443	29.257814370221652	21.458780277399658
32-33	21.755731613927313	27.44249728399486	29.088642838190616	21.713128263887214
34-35	21.84703882972084	27.57678836804648	29.12333746555445	21.452835336678227
36-37	21.84625405919047	27.52831418591064	29.074824173977287	21.550607580921604
38-39	21.686386789850147	27.661294772328127	29.110602706358122	21.5417157314636
40-41	21.984265787525036	27.495518698390903	28.965022762263615	21.555192751820446
42-43	21.934265875341797	27.635635245398856	28.662315197744885	21.76778368151446
44-45	22.068978550505527	27.751196172248804	28.456313298342966	21.7235119789027
46-47	21.999727088833556	27.761205088024255	28.186576058864453	22.052491764277732
48-49	22.044367036969497	27.905766510758895	28.100528135676512	21.949338316595092
50-51	22.010040075635708	28.006081901052692	27.986354528264613	21.997523495046988
52-53	22.178219144565105	27.922312111584187	27.832586197743947	22.06688254610676
54-55	22.12986802142083	27.85411524759146	27.935734361210173	22.080282369777535
56-57	22.118178908411817	27.977558557908022	27.853748729746364	22.050513803933793
58-59	22.097315019028244	28.026171159230135	27.857381025676897	22.019132796064728
60-61	22.241112464880626	27.977750467898066	27.829062168943935	21.952074898277374
62-63	22.138890486799102	28.017840505455883	27.776502321334828	22.06676668641019
64-65	22.137297490796684	27.97027700553743	27.791215648742053	22.101209854923834
66-67	22.198079709761576	28.02799370843924	27.622145867649557	22.151780714149627
68-69	22.163260109807283	28.013754899569886	27.702163977646173	22.12082101297666
70-71	22.176974084379967	27.781174802957693	27.69152438993661	22.35032672272573
72-73	22.198627242577942	27.862780643359297	27.683733365295428	22.254858748767337
74-75	22.06158830997987	27.96495710272185	27.679876541492852	22.293578045805422
76-77	22.166559384638436	27.935895344148054	27.537078910202673	22.360466361010843
78-79	22.21880930276981	27.98612399191715	27.50833371679262	22.286732988520413
80-81	22.147072062006398	27.97402620204432	27.58612709013018	22.2927746458191
82-83	22.199864364958653	27.96623900886027	27.65433642566295	22.17956020051813
84-85	22.35912772784385	27.91447129099927	27.539808850475683	22.18659213068119
86-87	22.180808496187495	27.967293516025066	27.564832931171214	22.287065056616225
88-89	22.280609498447813	27.914907442869065	27.554505792246424	22.249977266436698
90-91	22.240311509265545	27.95886915507037	27.602362019140198	22.198457316523886
92-93	22.26663616613783	27.958545640658745	27.610417061923425	22.164401131280002
94-95	22.122165463880364	28.044295852520385	27.602581544563385	22.230957139035866
96-97	22.207952005424158	28.13249237402491	27.72039758977554	21.93915803077539
98-99	23.291263837379983	30.072535767304153	27.876572046732363	18.7596283485835
100	22.170422544976937	31.108973793871268	21.781366973958296	24.939236687193496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2376.0
1	2041.5
2	2397.5
3	3330.0
4	4562.5
5	7154.5
6	9317.5
7	11045.5
8	12387.5
9	12647.5
10	12004.5
11	11163.0
12	12200.5
13	16023.0
14	24537.0
15	35623.5
16	44490.0
17	50003.5
18	51403.5
19	48647.0
20	43686.0
21	38698.0
22	35848.5
23	36946.5
24	42221.5
25	51414.0
26	64398.0
27	79553.0
28	94296.5
29	109890.0
30	124910.0
31	139314.5
32	156354.0
33	173964.5
34	191673.5
35	208555.5
36	226472.5
37	243757.5
38	255750.5
39	264629.5
40	275127.0
41	284666.5
42	290722.0
43	296846.0
44	303037.0
45	309493.5
46	313376.5
47	314309.5
48	313296.0
49	308437.0
50	300864.5
51	290442.5
52	276212.5
53	260037.5
54	243088.0
55	224676.5
56	205844.5
57	185786.5
58	165239.5
59	143765.0
60	120071.5
61	97401.5
62	76652.5
63	58795.5
64	44138.0
65	33070.5
66	25196.0
67	19340.5
68	14744.0
69	10571.0
70	7531.5
71	5440.0
72	3791.0
73	2575.0
74	1682.0
75	1109.5
76	732.5
77	460.5
78	295.0
79	195.0
80	139.0
81	106.0
82	74.5
83	44.0
84	28.0
85	20.0
86	16.5
87	14.5
88	11.5
89	8.5
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001218188884158844
4	0.011228523627898909
5	0.011202041260851978
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.296585271613196E-5
22-23	0.0
24-25	0.0056621871591926234
26-27	0.046087733104261557
28-29	0.08224302336171142
30-31	0.07451010056773393
32-33	0.0341175969934491
34-35	0.003147074527525434
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.863318011480044E-4
56-57	5.84311453875103E-4
58-59	2.1812202895075524E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.2906960478468374E-4
72-73	0.012338596979831864
74-75	0.027404229867736527
76-77	0.0022882129605322444
78-79	0.0
80-81	0.0
82-83	0.007484586103194154
84-85	0.04487523294580531
86-87	0.037830597916418435
88-89	0.009407823160799609
90-91	1.7613957904150375E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	94.0
20-21	411.0
22-23	944.0
24-25	2428.0
26-27	3471.0
28-29	6327.0
30-31	14679.0
32-33	18936.0
34-35	26132.0
36-37	37078.0
38-39	44622.0
40-41	47784.0
42-43	56781.0
44-45	67649.0
46-47	67197.0
48-49	57909.0
50-51	51404.0
52-53	50508.0
54-55	53029.0
56-57	54333.0
58-59	53829.0
60-61	52703.0
62-63	52172.0
64-65	54400.0
66-67	57106.0
68-69	57714.0
70-71	56980.0
72-73	54869.0
74-75	56581.0
76-77	55484.0
78-79	58566.0
80-81	59467.0
82-83	58078.0
84-85	58588.0
86-87	61089.0
88-89	64864.0
90-91	67855.0
92-93	71475.0
94-95	85525.0
96-97	243304.0
98-99	461965.0
100-101	5047865.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.47914403723442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27799338358065	78.07163593211169
2	6.961538898637041	12.040558502720861
3	1.6361339732186317	4.2447439660256014
4	0.553777780446857	1.9156091375953495
5	0.24266248593500384	1.0492622036803285
6	0.11857327511698426	0.6152469202684984
7	0.0655725994540678	0.39694635911589465
8	0.03995226547670242	0.2764030176618862
9	0.027029405841933113	0.21037318929408594
>10	0.07487682202586053	0.9467883965790526
>50	0.0014264207058830199	0.08231474391545102
>100	3.2805761527911713E-4	0.053754099589361344
>500	8.975530256420599E-5	0.04226387424403955
>1k	4.487664249525188E-5	0.05409965719789595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3241183523465694E-5
2	0.0	0.0	0.0	0.0	1.3241183523465694E-5
3	0.0	0.0	0.0	1.3241183523465694E-5	1.3241183523465694E-5
4	0.0	7.944710114079417E-5	0.0	2.6482367046931387E-5	1.3241183523465694E-5
5	0.0	7.944710114079417E-5	0.0	2.6482367046931387E-5	1.3241183523465694E-5
6	1.5889420228158834E-4	7.944710114079417E-5	0.0	2.6482367046931387E-5	1.3241183523465694E-5
7	1.5889420228158834E-4	7.944710114079417E-5	0.0	5.2964734093862775E-5	1.3241183523465694E-5
8	1.7213538580505402E-4	7.944710114079417E-5	0.0	5.2964734093862775E-5	2.6482367046931387E-5
9	1.7213538580505402E-4	1.4565301875812264E-4	0.0	5.2964734093862775E-5	6.620591761732847E-5
10-11	1.7213538580505402E-4	1.4565301875812264E-4	0.0	6.620591761732847E-5	7.944710114079417E-5
12-13	1.7213538580505402E-4	1.4565301875812264E-4	0.0	9.268828466425986E-5	9.268828466425986E-5
14-15	1.7213538580505402E-4	1.8537656932851972E-4	0.0	9.268828466425986E-5	1.9861775285198542E-4
16-17	2.383413034223825E-4	1.8537656932851972E-4	0.0	9.268828466425986E-5	2.5820307870758107E-4
18-19	2.383413034223825E-4	2.251001198989168E-4	0.0	9.268828466425986E-5	3.111678128014438E-4
20-21	2.515824869458482E-4	2.648236704693139E-4	0.0	9.268828466425986E-5	3.3102958808664236E-4
22-23	2.515824869458482E-4	2.9792662927797815E-4	0.0	9.268828466425986E-5	3.7075313865703944E-4
24-25	2.515824869458482E-4	3.111678128014438E-4	0.0	9.93088764259927E-5	4.3033846451263506E-4
26-27	2.515824869458482E-4	3.177884045631767E-4	0.0	1.5227361051985548E-4	4.502002397978336E-4
28-29	2.515824869458482E-4	3.177884045631767E-4	0.0	3.111678128014438E-4	4.6344142332129933E-4
30-31	2.515824869458482E-4	3.5751195513357377E-4	0.0	9.93088764259927E-4	4.6344142332129933E-4
32-33	2.5820307870758107E-4	3.7075313865703944E-4	0.0	0.0020457628543754497	4.76682606844765E-4
34-35	2.648236704693139E-4	4.2371787275090225E-4	0.0	0.003621463693667867	4.76682606844765E-4
36-37	2.648236704693139E-4	4.899237903682307E-4	0.0	0.006362388683025267	4.899237903682307E-4
38-39	2.648236704693139E-4	4.899237903682307E-4	0.0	0.010083161253119127	5.16406157415162E-4
40-41	2.648236704693139E-4	5.561297079855592E-4	0.0	0.014002551576064972	5.296473409386278E-4
42-43	2.648236704693139E-4	6.09094442079422E-4	0.0	0.017630635861494572	5.495091162238264E-4
44-45	2.7144426223104674E-4	7.216445020288803E-4	0.0	0.02206643234185558	5.561297079855592E-4
46-47	2.780648539927796E-4	7.679886443610102E-4	0.0	0.026422781721075796	5.62750299747292E-4
48-49	2.780648539927796E-4	7.679886443610102E-4	0.0	0.031116781280144383	5.693708915090248E-4
50-51	2.913060375162453E-4	8.937798878339343E-4	0.0	0.03554595716874366	5.693708915090248E-4
52-53	2.913060375162453E-4	9.004004795956672E-4	0.0	0.040120786076101055	6.223356256028876E-4
54-55	2.913060375162453E-4	9.136416631191329E-4	0.0	0.045006782796259893	6.753003596967505E-4
56-57	2.913060375162453E-4	9.268828466425987E-4	0.0	0.04978685004823101	6.95162134981949E-4
58-59	2.913060375162453E-4	9.335034384043315E-4	0.0	0.05462650262605773	7.150239102671475E-4
60-61	3.177884045631767E-4	9.599858054512629E-4	0.0	0.059618428814404295	7.150239102671475E-4
62-63	3.7075313865703944E-4	9.666063972129957E-4	0.0	0.06433891074051981	7.746092361227431E-4
64-65	3.7075313865703944E-4	0.0010990182324476527	0.0	0.06931759574534291	7.81229827884476E-4
66-67	3.773737304187723E-4	0.0011122594159711184	0.0	0.0744353131771624	7.878504196462088E-4
68-69	3.839943221805051E-4	0.0011122594159711184	0.0	0.07957951297602882	8.143327866931402E-4
70-71	4.0385609746570366E-4	0.0011188800077328513	0.0	0.08447875087971113	8.408151537400716E-4
72-73	4.369590562743679E-4	0.0011387417830180497	0.0	0.08843124416146564	9.136416631191329E-4
74-75	4.4357964803610074E-4	0.0011784653335884469	0.0	0.09137078690367503	9.136416631191329E-4
76-77	4.6344142332129933E-4	0.0012645330264909739	0.0	0.09427060609531401	9.268828466425987E-4
78-79	4.76682606844765E-4	0.0012843948017761723	0.0	0.09622368066502521	9.268828466425987E-4
80-81	4.76682606844765E-4	0.0012910153935379051	0.0	0.09675994859772556	9.798475807364614E-4
82-83	4.76682606844765E-4	0.001297635985299638	0.0	0.0971902870622382	0.001032812314830324
84-85	4.833031986064978E-4	0.0013108771688231037	0.0	0.09740876659037538	0.001039432906592057
86-87	4.899237903682307E-4	0.0013241183523465695	0.0	0.09748159309975443	0.0010526740901155227
88	4.899237903682307E-4	0.001430047820534295	0.0	0.0974948342832779	0.001085777048924187
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11280	0.0	51.344814	1
GTATCAA	20715	0.0	38.31947	1
ATCAACG	25120	0.0	31.258762	3
TCAACGC	25160	0.0	31.225887	4
CAACGCA	25610	0.0	30.690481	5
TATCAAC	26095	0.0	30.271406	2
AACGCAG	26180	0.0	30.083736	6
ACGCAGA	29970	0.0	26.236485	7
CGCAGAG	30240	0.0	26.016392	8
GTGGTAT	5095	0.0	25.896168	1
TGGTATC	5165	0.0	23.222914	2
GCAGAGT	34740	0.0	22.560099	9
GAGTACT	21365	0.0	20.53661	12-13
GTACATG	23725	0.0	20.02416	1
CAGAGTA	33815	0.0	19.941278	10-11
TACATGG	23450	0.0	19.857092	2
AGAGTAC	31710	0.0	19.30668	10-11
ACATGGG	24260	0.0	18.787968	3
GTACTTT	23710	0.0	18.5239	14-15
AGTACTT	22725	0.0	17.828178	12-13
>>END_MODULE