FastQCFastQC Report
Fri 27 May 2016
SRR2138647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9192237
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142200.051.2919771
GTATCAA259900.037.858551
TCAACGC315500.030.723844
ATCAACG315950.030.6260643
CAACGCA320900.030.1498745
TATCAAC323850.030.0910682
AACGCAG331000.029.3043526
ACGCAGA373650.025.924977
CGCAGAG381000.025.4248458
GTGGTAT71150.024.8586771
TGGTATC68450.024.2713072
GCAGAGT434300.022.1958439
GAGTACT267200.021.05000912-13
GTACATG293450.020.21761
TACATGG289250.019.91752
CAGAGTA426900.019.6348610-11
ACATGGG296000.019.144233
AGAGTAC399500.018.90271410-11
GTACTTT296150.018.86235614-15
AGTACTT290600.017.37592912-13
Produced by FastQC (version 0.11.5)