##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9192237
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.779656029321266	33.0	33.0	33.0	33.0	33.0
2	32.77780479332724	33.0	33.0	33.0	33.0	33.0
3	32.77629134235769	33.0	33.0	33.0	33.0	33.0
4	36.71976571100158	37.0	37.0	37.0	37.0	37.0
5	36.73468710608745	37.0	37.0	37.0	37.0	37.0
6	36.743979729852484	37.0	37.0	37.0	37.0	37.0
7	36.73142435296218	37.0	37.0	37.0	37.0	37.0
8	36.723059577336834	37.0	37.0	37.0	37.0	37.0
9	36.71938386706087	37.0	37.0	37.0	37.0	37.0
10-11	36.69259593720223	37.0	37.0	37.0	37.0	37.0
12-13	36.67095566617788	37.0	37.0	37.0	37.0	37.0
14-15	38.907322722423274	40.0	40.0	40.0	37.0	40.0
16-17	38.86023815530431	40.0	37.0	40.0	37.0	40.0
18-19	38.88917577951918	40.0	40.0	40.0	37.0	40.0
20-21	38.92590423417746	40.0	40.0	40.0	37.0	40.0
22-23	38.95453856602039	40.0	40.0	40.0	37.0	40.0
24-25	38.962005526944104	40.0	40.0	40.0	37.0	40.0
26-27	38.866154562283086	40.0	40.0	40.0	37.0	40.0
28-29	38.79483907910067	40.0	38.5	40.0	37.0	40.0
30-31	38.73187972623528	40.0	37.0	40.0	37.0	40.0
32-33	38.41828593962542	40.0	37.0	40.0	37.0	40.0
34-35	38.55274923779346	40.0	37.0	40.0	37.0	40.0
36-37	38.523722336784466	40.0	37.0	40.0	37.0	40.0
38-39	38.55548005585267	40.0	37.0	40.0	37.0	40.0
40-41	38.52287320889396	40.0	37.0	40.0	37.0	40.0
42-43	38.228958989756265	40.0	37.0	40.0	37.0	40.0
44-45	38.135288320065655	40.0	37.0	40.0	35.0	40.0
46-47	38.1579337749659	40.0	37.0	40.0	37.0	40.0
48-49	38.02868701388965	40.0	37.0	40.0	35.0	40.0
50-51	38.130815083716996	40.0	37.0	40.0	37.0	40.0
52-53	38.12089426782623	40.0	37.0	40.0	37.0	40.0
54-55	38.06314965156671	40.0	37.0	40.0	37.0	40.0
56-57	37.95724133345283	40.0	37.0	40.0	33.0	40.0
58-59	37.83414618993875	40.0	37.0	40.0	33.0	40.0
60-61	37.59805106034149	40.0	37.0	40.0	33.0	40.0
62-63	37.41392966969748	37.0	37.0	40.0	33.0	40.0
64-65	37.25039886391114	37.0	37.0	40.0	33.0	40.0
66-67	37.019605621308976	37.0	37.0	40.0	33.0	40.0
68-69	36.74960358865361	37.0	37.0	40.0	33.0	40.0
70-71	36.311592739238606	37.0	37.0	40.0	33.0	40.0
72-73	36.204489815297364	37.0	37.0	37.0	33.0	40.0
74-75	35.96950428616345	37.0	37.0	37.0	33.0	40.0
76-77	33.924237784583966	35.0	33.0	37.0	27.0	37.0
78-79	35.34311210642841	37.0	33.0	37.0	33.0	37.0
80-81	35.64183016783353	37.0	37.0	37.0	33.0	37.0
82-83	35.63544456699188	37.0	37.0	37.0	33.0	37.0
84-85	35.52477077221968	37.0	35.0	37.0	33.0	37.0
86-87	35.100698030085965	37.0	33.0	37.0	33.0	37.0
88-89	35.02460411435359	37.0	33.0	37.0	33.0	37.0
90-91	35.17966457324752	37.0	33.0	37.0	33.0	37.0
92-93	35.16567752264201	37.0	33.0	37.0	33.0	37.0
94-95	35.13563368547095	37.0	33.0	37.0	33.0	37.0
96-97	35.09323589437135	37.0	33.0	37.0	33.0	37.0
98-99	35.05580190250113	37.0	33.0	37.0	33.0	37.0
100	35.00112587640896	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	8.0
20	42.0
21	149.0
22	624.0
23	1903.0
24	4710.0
25	9820.0
26	17673.0
27	29144.0
28	44877.0
29	64080.0
30	89544.0
31	121273.0
32	163754.0
33	223902.0
34	321006.0
35	496808.0
36	973011.0
37	2708794.0
38	3776409.0
39	144702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52593106552844	20.29564729455953	14.810551555622423	23.367870084289603
2	14.576386574889225	20.804489701473102	41.99184594566045	22.627277777977223
3	18.096083746449544	26.110268058742008	30.539471441158287	25.25417675365016
4	12.440359011172152	18.16031254086769	38.96684954357657	30.432478904383586
5	13.341509940801377	37.62874321597439	35.41360646814403	13.6161403750802
6	29.640869790454705	37.80039613861131	18.833108850435426	13.725625220498557
7	26.334014233967206	32.97831637717783	22.89365472191372	17.794014666941248
8	24.318802920333756	36.51663898570065	21.050316696577774	18.11424139738782
9	25.408885780468886	17.201732287798933	21.533202418519018	35.85617951321316
10-11	24.14702210136662	26.902347056543473	28.60410365833692	20.346527183752986
12-13	25.02180916353658	24.520342545563174	28.8730915010133	21.584756789886946
14-15	22.156271645302443	25.8608813066939	26.886904678371543	25.095942369632112
16-17	21.4320464104657	29.008194632057467	27.524725483035304	22.03503347444153
18-19	21.40524118340291	27.95670955829359	29.588793239338802	21.049256018964698
20-21	22.277020967863535	27.00513347502872	29.621038642326564	21.096806914781183
22-23	21.988978004423466	27.01629200824811	29.45549113691556	21.53923885041287
24-25	21.771678992891562	27.189267043055455	29.50259438376856	21.536459580284426
26-27	21.651317396230084	27.350387836244316	29.466618638084757	21.53167612944084
28-29	21.547441060837347	27.41686973977429	29.501813596545727	21.53387560284263
30-31	21.838177074849206	27.216195996225366	29.340691149247043	21.60493577967838
32-33	21.755619816915406	27.294757321698178	29.24794047999619	21.70168238139023
34-35	21.82121409929177	27.480644666528203	29.297296356057206	21.400844878122825
36-37	21.79566921102589	27.45982874155776	29.10697233855658	21.637529708859766
38-39	21.63674059430462	27.534639582259864	29.229630758012586	21.59898906542293
40-41	21.921251556023282	27.39529813206099	29.08634157094244	21.597108740973287
42-43	21.960908176131433	27.520792283149774	28.76735919203454	21.75094034868425
44-45	22.005557231578234	27.6913074688311	28.55675698797665	21.746378311614016
46-47	22.023300333929445	27.67193317270962	28.247935589458432	22.0568309039025
48-49	22.102372440577128	27.76055941013716	28.123550516510875	22.01351763277484
50-51	22.000770334448145	27.916768969485783	28.05627071198601	22.026189984080062
52-53	22.069153663161842	27.880791114023896	27.97058383483121	22.07947138798306
54-55	22.117552887819794	27.806766697383335	27.95788144991418	22.11779896488269
56-57	22.18352004637808	27.925787990865803	27.847180877282067	22.04351108547405
58-59	22.07649001801411	28.061898875474263	27.947532362113087	21.914078744398545
60-61	22.26478447659475	27.98314328554899	27.814016360223633	21.938055877632635
62-63	22.111376915993418	28.069557216252285	27.812305840041667	22.006760027712634
64-65	22.09261534558944	28.004658787683002	27.803707944137397	22.099017922590168
66-67	22.17215481103756	27.940487658303358	27.68469461702297	22.20266291363611
68-69	22.152052099969218	27.900714274570383	27.787145534155687	22.16008809130471
70-71	22.14105361351709	27.69544937962436	27.769543376774124	22.39395363008442
72-73	22.24440739894292	27.731859954905136	27.75326562407602	22.270467022075927
74-75	22.083774320217632	27.875068315128875	27.718973881679908	22.322183482973575
76-77	22.223899718987276	27.764716352584205	27.62665580775631	22.384728120672204
78-79	22.235835479778533	27.778698028822262	27.588622597297174	22.39684389410203
80-81	22.14358633808861	27.814558833161012	27.705309421067398	22.336545407682976
82-83	22.19740071451736	27.778424628022734	27.79774461957641	22.226430037883496
84-85	22.382588906712083	27.7234370706984	27.67464300536376	22.21933101722576
86-87	22.2509658912112	27.747857317052972	27.714156147456094	22.28702064427973
88-89	22.341636520931843	27.65201083011513	27.724768301013157	22.281584347939866
90-91	22.319228241883426	27.674821061385167	27.71989296689276	22.286057729838642
92-93	22.37070915766964	27.570525347932257	27.82288616896848	22.235879325429625
94-95	22.176848736082114	27.644817653918857	27.835709968847393	22.342623641151636
96-97	22.248863515412868	27.798083554433937	27.8963893552186	22.0566635749346
98-99	23.431098372617463	29.668465210275347	28.045846224328447	18.854590192778748
100	22.326455671242794	30.740902963870454	21.88056594442056	25.052075420466192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1648.0
1	1578.5
2	2411.5
3	3620.0
4	5113.0
5	8325.0
6	11020.0
7	13203.5
8	15056.0
9	15392.5
10	14482.5
11	13446.0
12	14711.0
13	19383.5
14	29818.0
15	43823.5
16	55564.5
17	62732.5
18	64003.0
19	60361.0
20	54656.0
21	48537.0
22	44885.0
23	46375.0
24	52729.0
25	63660.5
26	78677.0
27	95255.5
28	112815.0
29	133522.0
30	153832.5
31	173889.0
32	195819.5
33	217078.0
34	237942.0
35	256101.0
36	274980.0
37	294569.5
38	309461.0
39	320005.0
40	331128.5
41	340872.5
42	345916.0
43	351725.0
44	357322.0
45	363715.5
46	366906.0
47	366506.5
48	367787.0
49	365262.0
50	357931.0
51	348372.5
52	336139.5
53	319158.5
54	300229.5
55	277575.5
56	253121.0
57	229321.0
58	204831.5
59	178740.0
60	148906.5
61	120979.0
62	95812.0
63	74139.5
64	56944.0
65	43148.0
66	32515.0
67	24759.5
68	18942.0
69	13644.5
70	9434.0
71	6754.0
72	4741.5
73	3183.5
74	2076.5
75	1274.5
76	835.0
77	549.0
78	365.5
79	247.0
80	167.5
81	112.5
82	76.5
83	53.5
84	39.0
85	26.0
86	20.0
87	16.0
88	10.0
89	6.0
90	4.5
91	4.0
92	3.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0011966619224460814
4	0.010780836046764242
5	0.010617654875521595
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.263699760509711E-5
22-23	0.0
24-25	0.005930601085463226
26-27	0.04779335765400744
28-29	0.08449873279128606
30-31	0.07681156821138177
32-33	0.03514095515562981
34-35	0.002984204414388487
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.9920484456807642E-4
56-57	4.2496531958365915E-4
58-59	2.379041783824562E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.5611858717924187E-4
72-73	0.011624087098289717
74-75	0.026468081772595818
76-77	0.0024777677962790844
78-79	0.0
80-81	0.0
82-83	0.007485360791759104
84-85	0.04526029215259689
86-87	0.03847399275388596
88-89	0.009656719207526011
90-91	7.532521834212892E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	461.0
22-23	1020.0
24-25	3300.0
26-27	4038.0
28-29	7119.0
30-31	16765.0
32-33	21323.0
34-35	29922.0
36-37	42717.0
38-39	51502.0
40-41	55988.0
42-43	66333.0
44-45	79604.0
46-47	77873.0
48-49	68019.0
50-51	60016.0
52-53	57189.0
54-55	61175.0
56-57	64884.0
58-59	64172.0
60-61	63872.0
62-63	63238.0
64-65	66426.0
66-67	71437.0
68-69	69805.0
70-71	67547.0
72-73	64574.0
74-75	66168.0
76-77	65838.0
78-79	69622.0
80-81	71261.0
82-83	69345.0
84-85	71664.0
86-87	74851.0
88-89	79977.0
90-91	84161.0
92-93	89021.0
94-95	101589.0
96-97	295674.0
98-99	565434.0
100-101	6187180.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.87429237459577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.65449125380171	73.47178228488606
2	7.594793476851737	12.588262702505673
3	2.0032858609045	4.9806269443948
4	0.7731739281472128	2.5630496871074726
5	0.3666850053036917	1.5194380169459172
6	0.20694343243059338	1.0290174314553242
7	0.12048162865565921	0.6989380803283602
8	0.06600360787715708	0.43760018375917503
9	0.04618170697769242	0.34445486577844725
>10	0.16490259013731537	2.0809542768636633
>50	0.0022893978675483854	0.12271647113449756
>100	7.21606805079548E-4	0.11650631241550471
>500	2.3252120272601063E-5	0.017458647305035303
>1k	2.3252119843244358E-5	0.029194095120005314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0878744749509831E-5
2	0.0	0.0	0.0	0.0	3.263623424852949E-5
3	0.0	0.0	0.0	0.0	3.263623424852949E-5
4	0.0	2.1757489499019662E-5	0.0	0.0	3.263623424852949E-5
5	0.0	5.4393723747549155E-5	0.0	2.1757489499019662E-5	3.263623424852949E-5
6	0.0	5.4393723747549155E-5	0.0	2.1757489499019662E-5	5.4393723747549155E-5
7	0.0	5.4393723747549155E-5	0.0	3.263623424852949E-5	5.4393723747549155E-5
8	0.0	6.527246849705899E-5	0.0	4.3514978998039324E-5	6.527246849705899E-5
9	0.0	8.702995799607865E-5	0.0	7.615121324656881E-5	8.702995799607865E-5
10-11	0.0	8.702995799607865E-5	0.0	9.790870274558847E-5	8.702995799607865E-5
12-13	0.0	8.702995799607865E-5	0.0	1.1422681986985323E-4	1.0334807512034339E-4
14-15	0.0	1.5774179886789255E-4	0.0	1.5774179886789255E-4	1.6318117124264748E-4
16-17	0.0	1.740599159921573E-4	0.0	1.740599159921573E-4	1.8493866074166713E-4
18-19	0.0	1.9037803311642204E-4	0.0	1.9037803311642204E-4	1.9581740549117694E-4
20-21	0.0	2.0669615024068678E-4	0.0	1.9581740549117694E-4	1.9581740549117694E-4
22-23	0.0	2.2301426736495152E-4	0.0	2.1213552261544171E-4	2.2845363973970646E-4
24-25	0.0	2.774079911125007E-4	0.0	2.5021112923872613E-4	2.7196861873774575E-4
26-27	0.0	3.154835977357851E-4	0.0	2.774079911125007E-4	2.7196861873774575E-4
28-29	0.0	3.3180171486004985E-4	0.0	5.058616308522071E-4	2.774079911125007E-4
30-31	0.0	3.807560662328441E-4	0.0	0.0011205107091995126	2.9372610823676543E-4
32-33	0.0	3.916348109823539E-4	0.0	0.002447717568639712	3.0460485298627524E-4
34-35	0.0	4.297104176056383E-4	0.0	0.004166559239062265	3.372410872348048E-4
36-37	0.0	4.895435137279424E-4	0.0	0.006543564966830163	3.372410872348048E-4
38-39	5.4393723747549155E-6	5.276191203512268E-4	0.0	0.010617654875521595	3.589985767338244E-4
40-41	1.0878744749509831E-5	5.928915888482858E-4	0.0	0.014669987294714006	3.589985767338244E-4
42-43	1.0878744749509831E-5	6.581640573453448E-4	0.0	0.018374199881922106	3.86195438607599E-4
44-45	1.0878744749509831E-5	7.615121324656882E-4	0.0	0.022829045856846378	4.1339230048137356E-4
46-47	1.0878744749509831E-5	8.159058562132374E-4	0.0	0.028110676432733406	4.2971041760563836E-4
48-49	1.0878744749509831E-5	8.159058562132374E-4	0.0	0.033163853368880725	4.3514978998039324E-4
50-51	1.0878744749509831E-5	8.811783247102963E-4	0.0	0.03832037838014837	4.3514978998039324E-4
52-53	1.0878744749509831E-5	8.811783247102963E-4	0.0	0.043253889124051086	4.949828861026973E-4
54-55	1.0878744749509831E-5	9.301326760830905E-4	0.0	0.0479698249729636	5.548159822250014E-4
56-57	2.1757489499019662E-5	9.736476550811299E-4	0.0	0.0532296980593516	5.711340993492661E-4
58-59	3.263623424852949E-5	0.0010008445169549045	0.0	0.05860923733798422	5.874522164735309E-4
60-61	3.263623424852949E-5	0.0010117232617044142	0.0	0.0639452616376188	5.874522164735309E-4
62-63	3.263623424852949E-5	0.0010280413788286789	0.0	0.06946622459799502	6.092097059725505E-4
64-65	3.263623424852949E-5	0.0011313894539490225	0.0	0.07468802207775975	6.092097059725505E-4
66-67	4.3514978998039324E-5	0.0011422681986985323	0.0	0.07977927462053035	6.146490783473054E-4
68-69	4.3514978998039324E-5	0.0011585863158227968	0.0	0.08521864699528525	6.200884507220603E-4
70-71	4.3514978998039324E-5	0.0011966619224460812	0.0	0.09003793091931811	6.200884507220603E-4
72-73	4.3514978998039324E-5	0.0012401769014441207	0.0	0.09403586961476298	7.179971534676488E-4
74-75	4.3514978998039324E-5	0.0012619343909431404	0.0	0.09682626764301225	7.179971534676488E-4
76-77	4.3514978998039324E-5	0.0013054493699411797	0.0	0.0996928168845081	7.179971534676488E-4
78-79	4.3514978998039324E-5	0.0013163281146906895	0.0	0.10138990106543164	7.179971534676488E-4
80-81	4.3514978998039324E-5	0.0013380856041897093	0.0	0.10204262575040221	7.343152705919136E-4
82-83	4.3514978998039324E-5	0.0013489643489392191	0.0	0.10232547311388947	7.506333877161783E-4
84-85	4.3514978998039324E-5	0.001354403721313974	0.0	0.1025267298917554	7.560727600909332E-4
86-87	4.3514978998039324E-5	0.0013707218384382386	0.0	0.10261919922212623	7.669515048404431E-4
88	4.3514978998039324E-5	0.001425115562185788	0.0	0.10263007796687575	7.72390877215198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14220	0.0	51.291977	1
GTATCAA	25990	0.0	37.85855	1
TCAACGC	31550	0.0	30.72384	4
ATCAACG	31595	0.0	30.626064	3
CAACGCA	32090	0.0	30.149874	5
TATCAAC	32385	0.0	30.091068	2
AACGCAG	33100	0.0	29.304352	6
ACGCAGA	37365	0.0	25.92497	7
CGCAGAG	38100	0.0	25.424845	8
GTGGTAT	7115	0.0	24.858677	1
TGGTATC	6845	0.0	24.271307	2
GCAGAGT	43430	0.0	22.195843	9
GAGTACT	26720	0.0	21.050009	12-13
GTACATG	29345	0.0	20.2176	1
TACATGG	28925	0.0	19.9175	2
CAGAGTA	42690	0.0	19.63486	10-11
ACATGGG	29600	0.0	19.14423	3
AGAGTAC	39950	0.0	18.902714	10-11
GTACTTT	29615	0.0	18.862356	14-15
AGTACTT	29060	0.0	17.375929	12-13
>>END_MODULE