FastQCFastQC Report
Fri 27 May 2016
SRR2138646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6895216
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107800.053.6169431
GTATCAA203500.038.3118021
TCAACGC246250.031.1212844
ATCAACG246950.030.9988463
TATCAAC251600.030.69822
CAACGCA252900.030.2989965
AACGCAG258800.029.68736
GTGGTAT50100.026.2354011
ACGCAGA295700.025.8813367
CGCAGAG297850.025.6945158
TGGTATC48500.024.982262
GCAGAGT331800.023.0138349
GAGTACT201400.021.48632612-13
CAGAGTA328400.020.1761510-11
GTACATG231700.020.0673871
CTTATAC42800.019.9065671
TACATGG227900.019.6129652
GTACTTT222700.019.36422214-15
AGAGTAC305850.019.3056210-11
ACATGGG230400.019.0656623
Produced by FastQC (version 0.11.5)