FastQCFastQC Report
Fri 27 May 2016
SRR2138645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7848269
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.052.613351
GTATCAA211050.038.1555821
CTTATAC59300.037.5807381
TCAACGC262200.030.1724224
ATCAACG262900.030.0761643
TATCAAC268600.029.6772482
CAACGCA268350.029.4133055
TATACAC90950.028.8380173
AACGCAG277350.028.5482716
TGGTATC49450.025.47992
GTGGTAT52700.025.453671
ACGCAGA321300.024.5631967
CGCAGAG324700.024.2927978
TTATACA100850.023.9248472
ACACATC108400.021.5018396
TACACAT117700.021.334235
GCAGAGT370350.021.229019
ACATCTC112400.020.355468
GAGTACT217800.020.12443712-13
GTACATG261500.019.3550761
Produced by FastQC (version 0.11.5)