##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7848269
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.774270351844464	33.0	33.0	33.0	33.0	33.0
2	32.77356777653773	33.0	33.0	33.0	33.0	33.0
3	32.772993509779035	33.0	33.0	33.0	33.0	33.0
4	36.713902390450684	37.0	37.0	37.0	37.0	37.0
5	36.72807672621823	37.0	37.0	37.0	37.0	37.0
6	36.738182903771516	37.0	37.0	37.0	37.0	37.0
7	36.72541461053387	37.0	37.0	37.0	37.0	37.0
8	36.715927550393594	37.0	37.0	37.0	37.0	37.0
9	36.71506009796555	37.0	37.0	37.0	37.0	37.0
10-11	36.684979579573536	37.0	37.0	37.0	37.0	37.0
12-13	36.664044899582315	37.0	37.0	37.0	37.0	37.0
14-15	38.88988552762399	40.0	38.5	40.0	37.0	40.0
16-17	38.83849992909265	40.0	37.0	40.0	37.0	40.0
18-19	38.86706164123579	40.0	38.5	40.0	37.0	40.0
20-21	38.90209399033877	40.0	40.0	40.0	37.0	40.0
22-23	38.92926298082395	40.0	40.0	40.0	37.0	40.0
24-25	38.93842273271265	40.0	40.0	40.0	37.0	40.0
26-27	38.84328800526913	40.0	40.0	40.0	37.0	40.0
28-29	38.76471384455071	40.0	37.0	40.0	37.0	40.0
30-31	38.70340313503416	40.0	37.0	40.0	37.0	40.0
32-33	38.39072459836421	40.0	37.0	40.0	37.0	40.0
34-35	38.52101148576638	40.0	37.0	40.0	37.0	40.0
36-37	38.49058198961276	40.0	37.0	40.0	37.0	40.0
38-39	38.50882671997964	40.0	37.0	40.0	37.0	40.0
40-41	38.483990145986695	40.0	37.0	40.0	37.0	40.0
42-43	38.19722841509477	40.0	37.0	40.0	37.0	40.0
44-45	38.09529489212944	40.0	37.0	40.0	35.0	40.0
46-47	38.11789963987502	40.0	37.0	40.0	35.0	40.0
48-49	37.981051411498534	40.0	37.0	40.0	33.0	40.0
50-51	38.0645111798117	40.0	37.0	40.0	37.0	40.0
52-53	38.0513073840482	40.0	37.0	40.0	37.0	40.0
54-55	37.98968482038747	40.0	37.0	40.0	33.0	40.0
56-57	37.87495545101241	40.0	37.0	40.0	33.0	40.0
58-59	37.74680128488711	40.0	37.0	40.0	33.0	40.0
60-61	37.502901102514414	38.5	37.0	40.0	33.0	40.0
62-63	37.310019026120116	37.0	37.0	40.0	33.0	40.0
64-65	37.14160454616075	37.0	37.0	40.0	33.0	40.0
66-67	36.90768152419291	37.0	37.0	40.0	33.0	40.0
68-69	36.63753912409537	37.0	37.0	40.0	33.0	40.0
70-71	36.203444825431546	37.0	37.0	38.5	33.0	40.0
72-73	36.092058541893294	37.0	37.0	37.0	33.0	40.0
74-75	35.862197905538345	37.0	37.0	37.0	33.0	40.0
76-77	33.821738007934854	35.0	33.0	37.0	27.0	37.0
78-79	35.247964065777765	37.0	33.0	37.0	33.0	37.0
80-81	35.56037278260135	37.0	35.0	37.0	33.0	37.0
82-83	35.56515036107406	37.0	37.0	37.0	33.0	37.0
84-85	35.46445755471762	37.0	33.0	37.0	33.0	37.0
86-87	35.03714796630072	37.0	33.0	37.0	33.0	37.0
88-89	34.96481533503177	37.0	33.0	37.0	33.0	37.0
90-91	35.12836632773943	37.0	33.0	37.0	33.0	37.0
92-93	35.11871679635311	37.0	33.0	37.0	33.0	37.0
94-95	35.09256622011037	37.0	33.0	37.0	33.0	37.0
96-97	35.05013223019748	37.0	33.0	37.0	33.0	37.0
98-99	35.015355272389456	37.0	33.0	37.0	33.0	37.0
100	34.95912664630752	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	3.0
19	9.0
20	25.0
21	147.0
22	552.0
23	1752.0
24	4184.0
25	8560.0
26	16052.0
27	26409.0
28	39948.0
29	56917.0
30	78464.0
31	106430.0
32	143526.0
33	196837.0
34	281337.0
35	436301.0
36	861145.0
37	2377569.0
38	3096793.0
39	115305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76077552897333	19.91167733929609	14.657079159748474	23.670467971982102
2	14.749863950891593	20.632562415992624	41.6349388635889	22.982634769526886
3	18.291087249112312	25.637959767248038	30.53101590521016	25.539937078429492
4	12.679349592791445	17.98253522512336	38.16764496709952	31.17047021498568
5	13.619291340188107	37.37818858176287	35.13160322635235	13.870916851696668
6	30.183598446995124	37.290120917109235	18.45627360632007	14.070007029575565
7	26.649761877428002	32.779266357970144	22.288265093869743	18.282706670732107
8	24.705141987360523	36.17467749894913	20.906597365610175	18.21358314808017
9	25.63343585700235	16.922190103320872	20.90718348211561	36.53719055756117
10-11	24.411344718179258	26.68153321452157	28.23435333319997	20.6727687340992
12-13	25.26635491214687	24.18632057591298	28.46773600650029	22.07958850543986
14-15	22.473726372019105	25.566331887961535	26.343847286580008	25.616094453439352
16-17	21.91514460067564	28.807957525411016	26.950293115590203	22.32660475832314
18-19	21.88955297021547	27.610961092184787	28.95576081808613	21.543725119513617
20-21	22.760501709237207	26.724535512869597	28.979905496769504	21.5350572811237
22-23	22.365427002282615	26.831573958905295	28.816695233856137	21.98630380495595
24-25	22.214581076917145	27.005215926084187	28.938839928839844	21.841363068158827
26-27	22.135724380363467	27.137982010387653	28.913977918592664	21.812315690656217
28-29	21.825759914694164	27.07898034604498	28.998821274605103	22.09643846465576
30-31	22.384242314434346	26.83985092879457	28.99929302723998	21.776613729531107
32-33	22.10749053723259	27.179082157468105	28.688262316697372	22.02516498860194
34-35	22.10023750961731	27.31744041051634	28.931000253341026	21.651321826525326
36-37	22.30609169992662	27.106328644123767	28.696011610934846	21.891568045014765
38-39	22.03625663044754	27.115682982570654	28.843357687407234	22.004702699574572
40-41	22.27589101476867	27.025553146506898	28.698765858351187	21.999789980373244
42-43	22.39056031881535	27.07174758567194	28.511858746041106	22.0258333494716
44-45	22.28420468788125	27.296411565095642	28.30087560799518	22.118508139027927
46-47	22.500886143954478	27.122443643472742	27.870170216131324	22.506499996441455
48-49	22.403281034874613	27.2725894485572	27.90733805397785	22.41679146259034
50-51	22.27573372748872	27.499624058752943	27.881952082951056	22.342690130807277
52-53	22.42408316831252	27.484021376681824	27.832790007522558	22.2591054474831
54-55	22.38600393286209	27.40667194391664	27.707083303587716	22.500240819633564
56-57	22.574628888746915	27.62400736265387	27.45202792421163	22.34933582438758
58-59	22.322970930753737	27.8015148362865	27.726736310901472	22.148777922058294
60-61	22.602737994004325	27.824579232947528	27.429313958959174	22.14336881408898
62-63	22.386976887977273	27.973396000431567	27.377578735955815	22.26204837563535
64-65	22.367418837307817	27.83638174752399	27.446048361372398	22.35015105379579
66-67	22.512942315621668	27.744662614226144	27.299569218590804	22.442825851561388
68-69	22.485449353370598	27.600692340586846	27.45593114553766	22.457927160504894
70-71	22.52927220304406	27.331411651867686	27.450417830760284	22.688898314327965
72-73	22.572784453736563	27.40228054715445	27.418884096099976	22.606050903009013
74-75	22.38171984756313	27.55388928332401	27.408607618730628	22.655783250382232
76-77	22.558882794649417	27.473075866011786	27.280048574145678	22.68799276519312
78-79	22.586645271945223	27.553236068357535	27.236906138120816	22.623212521576427
80-81	22.535205314023585	27.473183445453735	27.274473984816	22.71713725570668
82-83	22.57276496023277	27.456146007721642	27.378108511077002	22.59298052096858
84-85	22.688302770878526	27.415061902038502	27.31911848349997	22.577516843583
86-87	22.56089492783902	27.423293346603156	27.367612014758873	22.64819971079895
88-89	22.657139837253553	27.40798880420584	27.299110601481313	22.635760757059295
90-91	22.631006864387132	27.343022117538446	27.419677661778657	22.60629335629576
92-93	22.66910444415326	27.306043353404704	27.384639006311307	22.640213196130734
94-95	22.525566227018462	27.40814121231603	27.395273217299724	22.67101934336578
96-97	22.579713393119185	27.47926293179758	27.518847699315756	22.422175975767473
98-99	23.692437420119365	29.411262885140772	27.657512759665437	19.23878693507443
100	22.642304793224294	30.484562995140234	21.497375031151723	25.37575718048375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1575.0
1	1427.5
2	1835.0
3	2636.5
4	3802.5
5	6301.5
6	8425.5
7	10253.0
8	11947.5
9	12464.0
10	11876.5
11	11079.0
12	12219.5
13	16009.5
14	24361.5
15	35519.5
16	44557.5
17	50113.5
18	51370.0
19	48325.5
20	42988.5
21	37791.0
22	34713.5
23	35405.0
24	40179.0
25	49175.5
26	62147.0
27	76246.5
28	89456.0
29	103954.5
30	117864.5
31	131270.0
32	147756.5
33	165487.0
34	183711.0
35	200639.5
36	218917.5
37	234996.0
38	244919.5
39	254541.0
40	267940.0
41	279729.0
42	287135.5
43	295635.5
44	303818.5
45	314085.5
46	325792.5
47	335236.0
48	336389.5
49	331447.0
50	325443.5
51	315039.0
52	301996.0
53	286518.0
54	270797.5
55	253655.5
56	233472.0
57	212428.0
58	191393.5
59	168844.5
60	143170.0
61	117762.0
62	93436.0
63	71849.0
64	54066.0
65	39757.0
66	29583.0
67	22454.0
68	17182.5
69	12465.0
70	8599.5
71	5843.5
72	3854.5
73	2510.0
74	1637.5
75	1074.0
76	693.5
77	447.5
78	301.0
79	207.0
80	145.5
81	104.5
82	74.5
83	51.0
84	34.0
85	27.0
86	22.0
87	17.0
88	15.0
89	13.0
90	10.5
91	8.5
92	6.5
93	3.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0010957830319016843
4	0.010397197139904352
5	0.011378305203351211
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.822583940598575E-5
22-23	0.0
24-25	0.0057990316891591565
26-27	0.045195939377519964
28-29	0.08028311597217541
30-31	0.07251653838642753
32-33	0.033368234489719296
34-35	0.0032315806475254074
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.3979137876000588E-4
56-57	5.24914406144648E-4
58-59	6.965212386042161E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.1767484219987527E-4
72-73	0.012551241630836532
74-75	0.02793356688608057
76-77	0.002263844427403565
78-79	0.0
80-81	0.0
82-83	0.007550194046970742
84-85	0.04350550672870549
86-87	0.036587824979983495
88-89	0.009721179620378342
90-91	1.2938992730146068E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	108.0
20-21	389.0
22-23	916.0
24-25	2474.0
26-27	3391.0
28-29	5858.0
30-31	13852.0
32-33	17734.0
34-35	24672.0
36-37	35427.0
38-39	42825.0
40-41	45777.0
42-43	54257.0
44-45	66634.0
46-47	65668.0
48-49	57290.0
50-51	50394.0
52-53	49254.0
54-55	56101.0
56-57	61583.0
58-59	60413.0
60-61	60479.0
62-63	61589.0
64-65	64639.0
66-67	70686.0
68-69	62532.0
70-71	58803.0
72-73	56563.0
74-75	58024.0
76-77	57544.0
78-79	60924.0
80-81	62705.0
82-83	60599.0
84-85	61884.0
86-87	64764.0
88-89	69972.0
90-91	73595.0
92-93	77273.0
94-95	87862.0
96-97	253716.0
98-99	480656.0
100-101	5228443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.50388588596137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.1551133405895	77.08612523109677
2	6.91164707210665	11.819453650748917
3	1.6565809882976839	4.249323353527747
4	0.5706567377867677	1.9517347435109904
5	0.2636690633965238	1.1272364754157342
6	0.1445155254677498	0.7413983399006533
7	0.08781585280990307	0.5256017660345433
8	0.053038804156882	0.3628019086526313
9	0.034353065744307874	0.2643588551910806
>10	0.12023193677008401	1.5549607063852056
>50	0.0017414786330912629	0.101817317227819
>100	4.706788457109896E-4	0.07478816005196288
>500	1.1818255138087758E-4	0.06738054839569521
>1k	4.7272843760112984E-5	0.07301894386014465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2741663161647492E-5	0.0	1.2741663161647492E-5	0.0
3	0.0	1.2741663161647492E-5	0.0	2.5483326323294985E-5	0.0
4	0.0	5.096665264658997E-5	0.0	7.644997896988495E-5	0.0
5	0.0	7.644997896988495E-5	0.0	7.644997896988495E-5	0.0
6	0.0	7.644997896988495E-5	0.0	7.644997896988495E-5	1.2741663161647492E-5
7	0.0	7.644997896988495E-5	0.0	7.644997896988495E-5	1.2741663161647492E-5
8	0.0	8.919164213153244E-5	0.0	1.1467496845482743E-4	1.2741663161647492E-5
9	0.0	1.656416211014174E-4	0.0	1.1467496845482743E-4	2.5483326323294985E-5
10-11	0.0	1.656416211014174E-4	0.0	1.3378746319729867E-4	2.5483326323294985E-5
12-13	0.0	1.7201245268224114E-4	0.0	1.4652912635894615E-4	2.5483326323294985E-5
14-15	0.0	3.057999158795398E-4	0.0	1.528999579397699E-4	3.185415790411873E-5
16-17	6.370831580823746E-6	3.057999158795398E-4	0.0	1.7838328426306489E-4	3.8224989484942474E-5
18-19	1.2741663161647492E-5	3.376540737836585E-4	0.0	2.0386661058635988E-4	3.8224989484942474E-5
20-21	1.2741663161647492E-5	3.6950823168777725E-4	0.0	2.1660827374800736E-4	3.8224989484942474E-5
22-23	1.2741663161647492E-5	4.5869987381930974E-4	0.0	2.6757492639459734E-4	5.096665264658997E-5
24-25	1.2741663161647492E-5	5.03295694885076E-4	0.0	2.803165895562448E-4	6.370831580823746E-5
26-27	1.2741663161647492E-5	5.351498527891947E-4	0.0	3.3765407378365855E-4	6.370831580823746E-5
28-29	1.2741663161647492E-5	5.351498527891947E-4	0.0	4.969248633042522E-4	7.00791473890612E-5
30-31	1.2741663161647492E-5	5.861165054357846E-4	0.0	9.683664002852094E-4	7.644997896988495E-5
32-33	1.2741663161647492E-5	5.988581685974321E-4	0.0	0.0019112494742471236	8.919164213153244E-5
34-35	1.2741663161647492E-5	7.390164633755545E-4	0.0	0.003115336643022812	8.919164213153244E-5
36-37	1.2741663161647492E-5	8.664330949920294E-4	0.0	0.005294161043664532	8.919164213153244E-5
38-39	1.2741663161647492E-5	8.982872528961481E-4	0.0	0.008377643528783226	1.1467496845482743E-4
40-41	1.2741663161647492E-5	9.556247371235619E-4	0.0	0.011766925929781458	1.1467496845482743E-4
42-43	1.2741663161647492E-5	0.0010129622213509756	0.0	0.014780329267511091	1.1467496845482743E-4
44-45	1.2741663161647492E-5	0.0011722330108715692	0.0	0.0185391199001971	1.2741663161647493E-4
46-47	1.2741663161647492E-5	0.0012741663161647493	0.0	0.022794835396187364	1.5927078952059364E-4
48-49	1.2741663161647492E-5	0.0012805371477455731	0.0	0.026808459292106325	2.1660827374800736E-4
50-51	1.2741663161647492E-5	0.001458920432008638	0.0	0.030599104082696452	2.229791053288311E-4
52-53	1.2741663161647492E-5	0.0014652912635894615	0.0	0.03464458213651953	2.357207684904786E-4
54-55	1.2741663161647492E-5	0.0014652912635894615	0.0	0.038760139337731675	2.4209160007130235E-4
56-57	1.2741663161647492E-5	0.0014907745899127565	0.0	0.04328342976011653	2.484624316521261E-4
58-59	1.2741663161647492E-5	0.0014971454214935801	0.0	0.04753914525610679	2.5483326323294986E-4
60-61	1.2741663161647492E-5	0.001579966232044289	0.0	0.05212614399429989	2.6757492639459734E-4
62-63	1.2741663161647492E-5	0.0016500453794333503	0.0	0.056451938637679215	2.930582527178923E-4
64-65	1.2741663161647492E-5	0.0018985078110854764	0.0	0.060809587438962656	2.930582527178923E-4
66-67	2.5483326323294985E-5	0.0019176203058279477	0.0	0.06517997790340774	2.9942908429871605E-4
68-69	2.5483326323294985E-5	0.002000441116378656	0.0	0.06978608913634332	3.185415790411873E-4
70-71	2.5483326323294985E-5	0.0020322952742827752	0.0	0.07416285043236923	3.2491241062201107E-4
72-73	2.5483326323294985E-5	0.0020450369374444225	0.0	0.07791527023347442	3.8224989484942474E-4
74-75	2.5483326323294985E-5	0.002089632758510189	0.0	0.08087770691855745	3.8224989484942474E-4
76-77	2.5483326323294985E-5	0.0021724535690608973	0.0	0.08369361447728155	3.8224989484942474E-4
78-79	2.5483326323294985E-5	0.0022170493901266636	0.0	0.08563034727785196	3.8224989484942474E-4
80-81	2.5483326323294985E-5	0.0022170493901266636	0.0	0.08630565542541929	4.01362389591896E-4
82-83	2.5483326323294985E-5	0.0022170493901266636	0.0	0.08664330949920294	4.714415369809572E-4
84-85	2.5483326323294985E-5	0.00226164521119243	0.0	0.08683443444662765	4.714415369809572E-4
86-87	2.5483326323294985E-5	0.0023253535270006676	0.0	0.08687903026769342	4.714415369809572E-4
88	2.5483326323294985E-5	0.002408174337551376	0.0	0.08688540109927424	4.714415369809572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11640	0.0	52.61335	1
GTATCAA	21105	0.0	38.155582	1
CTTATAC	5930	0.0	37.580738	1
TCAACGC	26220	0.0	30.172422	4
ATCAACG	26290	0.0	30.076164	3
TATCAAC	26860	0.0	29.677248	2
CAACGCA	26835	0.0	29.413305	5
TATACAC	9095	0.0	28.838017	3
AACGCAG	27735	0.0	28.548271	6
TGGTATC	4945	0.0	25.4799	2
GTGGTAT	5270	0.0	25.45367	1
ACGCAGA	32130	0.0	24.563196	7
CGCAGAG	32470	0.0	24.292797	8
TTATACA	10085	0.0	23.924847	2
ACACATC	10840	0.0	21.501839	6
TACACAT	11770	0.0	21.33423	5
GCAGAGT	37035	0.0	21.22901	9
ACATCTC	11240	0.0	20.35546	8
GAGTACT	21780	0.0	20.124437	12-13
GTACATG	26150	0.0	19.355076	1
>>END_MODULE