FastQCFastQC Report
Fri 27 May 2016
SRR2138644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7062711
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111500.051.2037851
GTATCAA201900.037.4919551
ATCAACG250300.029.5603143
TCAACGC250650.029.5190354
TATCAAC256600.029.067362
CAACGCA256100.028.9221235
AACGCAG263300.028.2424746
TGGTATC51250.027.2254262
GTGGTAT57950.026.286671
ACGCAGA301100.024.6119087
CGCAGAG304150.024.3089928
CTTATAC46150.024.0393331
GCAGAGT342000.021.5063849
GAGTACT213250.020.27640512-13
GTACATG233300.019.7345621
TACATGG229650.019.4908082
TATACAC71500.019.3953723
CAGAGTA336350.019.22932210-11
AGAGTAC317600.018.63168710-11
GTACTTT234100.018.40729714-15
Produced by FastQC (version 0.11.5)