FastQCFastQC Report
Fri 27 May 2016
SRR2138643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7553923
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC200750.056.760191
TTATACA236750.048.5329972
TATACAC245300.047.366533
GGTATCA120650.047.2906381
GTATCAA221350.036.364871
TACACAT350450.033.1134875
ATACACA368800.031.9549854
ATCAACG273150.029.1491743
TCAACGC273550.029.121164
TATCAAC275150.029.0732212
CAACGCA276150.028.8126875
AACGCAG279350.028.553986
ACACATC413400.027.7868126
ACATCTC426900.027.0054178
CACATCT428300.026.6649467
CGCAGAG323450.024.5581028
ACGCAGA328250.024.2243027
CATCTCC477450.023.9895959
GCAGAGT365550.021.6161239
GTGGTAT61000.021.4591561
Produced by FastQC (version 0.11.5)