##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7553923
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75370969494924	33.0	33.0	33.0	33.0	33.0
2	32.765009651276564	33.0	33.0	33.0	33.0	33.0
3	32.762684237051396	33.0	33.0	33.0	33.0	33.0
4	36.69695600550866	37.0	37.0	37.0	37.0	37.0
5	36.70560210899688	37.0	37.0	37.0	37.0	37.0
6	36.71906637120871	37.0	37.0	37.0	37.0	37.0
7	36.70136788526968	37.0	37.0	37.0	37.0	37.0
8	36.693856556387985	37.0	37.0	37.0	37.0	37.0
9	36.69654469604734	37.0	37.0	37.0	37.0	37.0
10-11	36.66061091700299	37.0	37.0	37.0	37.0	37.0
12-13	36.63629984049348	37.0	37.0	37.0	37.0	37.0
14-15	38.817931292124634	40.0	37.0	40.0	37.0	40.0
16-17	38.74707737952849	40.0	37.0	40.0	37.0	40.0
18-19	38.777838945406245	40.0	37.0	40.0	37.0	40.0
20-21	38.82846680034299	40.0	37.0	40.0	37.0	40.0
22-23	38.85129506209938	40.0	37.0	40.0	37.0	40.0
24-25	38.85136266777111	40.0	37.0	40.0	37.0	40.0
26-27	38.71564835726753	40.0	37.0	40.0	37.0	40.0
28-29	38.574019575471844	40.0	37.0	40.0	37.0	40.0
30-31	38.540396545475375	40.0	37.0	40.0	37.0	40.0
32-33	38.202885432483136	40.0	37.0	40.0	37.0	40.0
34-35	38.304259010047666	40.0	37.0	40.0	37.0	40.0
36-37	38.27396955796682	40.0	37.0	40.0	37.0	40.0
38-39	38.24642946248634	40.0	37.0	40.0	37.0	40.0
40-41	38.25834401661777	40.0	37.0	40.0	37.0	40.0
42-43	38.007986713837056	40.0	37.0	40.0	33.0	40.0
44-45	37.914578322302944	40.0	37.0	40.0	35.0	40.0
46-47	37.93152425747909	40.0	37.0	40.0	33.0	40.0
48-49	37.8330170848903	40.0	37.0	40.0	33.0	40.0
50-51	37.80183931557842	40.0	37.0	40.0	33.0	40.0
52-53	37.80496158081573	40.0	37.0	40.0	33.0	40.0
54-55	37.75905462710706	40.0	37.0	40.0	33.0	40.0
56-57	37.608210812954184	40.0	37.0	40.0	33.0	40.0
58-59	37.49629766348535	40.0	37.0	40.0	33.0	40.0
60-61	37.24138475108086	37.0	37.0	40.0	33.0	40.0
62-63	37.04810387642273	37.0	37.0	40.0	33.0	40.0
64-65	36.923045507712935	37.0	37.0	40.0	33.0	40.0
66-67	36.72043760842013	37.0	37.0	40.0	33.0	40.0
68-69	36.481913653109444	37.0	37.0	40.0	33.0	40.0
70-71	36.05440994368303	37.0	37.0	38.5	33.0	40.0
72-73	35.97720319634992	37.0	37.0	37.0	33.0	40.0
74-75	35.76436128157287	37.0	37.0	37.0	33.0	40.0
76-77	33.706634711574345	35.0	33.0	37.0	27.0	37.0
78-79	35.16055066351621	37.0	33.0	37.0	33.0	37.0
80-81	35.49769190146421	37.0	35.0	37.0	33.0	37.0
82-83	35.51882217636809	37.0	37.0	37.0	33.0	37.0
84-85	35.43172784454498	37.0	33.0	37.0	33.0	37.0
86-87	35.00300193304538	37.0	33.0	37.0	33.0	37.0
88-89	34.93645217444552	37.0	33.0	37.0	33.0	37.0
90-91	35.12025565921526	37.0	33.0	37.0	33.0	37.0
92-93	35.12337025508578	37.0	33.0	37.0	33.0	37.0
94-95	35.10754738757071	37.0	33.0	37.0	33.0	37.0
96-97	35.068183524206745	37.0	33.0	37.0	33.0	37.0
98-99	35.03932606048245	37.0	33.0	37.0	33.0	37.0
100	34.987591050504726	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	14.0
20	54.0
21	319.0
22	1088.0
23	2945.0
24	6573.0
25	12366.0
26	20887.0
27	32779.0
28	46507.0
29	64001.0
30	84665.0
31	110780.0
32	146805.0
33	198079.0
34	280452.0
35	436912.0
36	862524.0
37	2327666.0
38	2805104.0
39	113399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.52742171187077	20.607186491045777	14.950523059342807	24.91486873774064
2	13.965816172603295	19.9388317831675	42.324233381780566	23.771118662448636
3	17.25318378268822	26.47600309723718	31.71146712481842	24.55934599525618
4	12.119057268229042	18.777646867082275	36.10855769792181	32.994738166766865
5	13.191939396394744	37.40058693921237	35.06839989291788	14.339073771475007
6	29.52878921323397	36.93621182000399	18.219738273742	15.315260693020038
7	25.643549715823156	33.14980838433222	21.282160276190265	19.92448162365436
8	25.132570189026282	35.5801746986301	21.127326820779082	18.15992829156453
9	24.677945486073924	16.424723948073076	19.86514821504005	39.03218235081295
10-11	24.06478726351857	26.116270976021333	27.73950700847758	22.079434751982514
12-13	25.239898526897882	23.549664194353053	27.593662259993913	23.616775018755156
14-15	22.830349210602225	25.305692154924003	24.70709590235431	27.156862732119457
16-17	22.503207935797068	29.12762679736079	25.281691380756726	23.087473886085416
18-19	22.33434865565879	27.022078461747622	27.16383923955804	23.479733643035548
20-21	23.316092459975206	26.264791484500094	27.146372896658683	23.27274315886602
22-23	23.134649413696412	27.663484598571088	26.90717716563345	22.29468882209905
24-25	23.43504378670674	27.830115563216317	27.07197011807801	21.662870531998934
26-27	23.200983381092772	28.147315799259538	27.07289071819129	21.5788101014564
28-29	22.299338364982585	26.777890852273313	27.622806031479808	23.299964751264294
30-31	23.799684771331723	26.89795255420748	27.652092254586197	21.650270419874598
32-33	23.839479893528196	26.788536453230392	28.15105692211466	21.220926731126752
34-35	22.727993236640753	27.30284486768314	28.736094237455873	21.233067658220232
36-37	23.122463332987632	26.441109771726868	27.8429223852046	22.5935045100809
38-39	22.575056930576366	25.79560653314324	28.51231522308874	23.117021313191657
40-41	22.091879484849223	25.78238127182789	29.169335057583595	22.956404185739295
42-43	22.77245595315867	25.516159888807465	29.445814408183534	22.265569749850332
44-45	21.953560979328614	26.34099306640245	28.817716896503313	22.887729057765615
46-47	23.367036656637772	25.329034769940108	27.492694628493602	23.811233944928524
48-49	22.784881480087908	25.590091127970222	28.103181129259667	23.521846262682207
50-51	22.297536576249865	26.46468699696836	28.168705164175524	23.069071262606254
52-53	22.07753824619587	27.22587626045093	28.709740030976548	21.986845462376646
54-55	21.983159744679067	27.471201241195615	27.512239607985677	23.03339940613964
56-57	23.071399984495436	27.8910480484692	26.494655394936277	22.54289657209908
58-59	21.748125094272694	28.818478437440024	27.86851479219006	21.564881676097222
60-61	22.927142549773542	29.455194144303675	25.942512181235998	21.67515112468678
62-63	22.017942347027436	29.965596257184853	25.995386060552217	22.021075335235498
64-65	22.23087189047012	29.19581367180659	26.33171932244549	22.241595115277796
66-67	22.54558333484961	28.341232348518147	26.478084661992696	22.635099654639543
68-69	22.645707749476333	27.550590104449196	26.92762509625133	22.87607704982314
70-71	22.772243609095746	27.189493509286685	26.995276910135008	23.042985971482562
72-73	22.86290277059284	27.152935449788856	26.899333001737887	23.084828777880414
74-75	22.764583365981935	27.338164404645788	26.913144549406965	22.984107679965312
76-77	23.026194138060717	27.175599845643166	26.703216167744802	23.094989848551315
78-79	22.8799014060569	27.15101596002265	26.7903589062085	23.178723727711954
80-81	22.874013750631157	27.195943905487102	26.878770319040733	23.05127202484101
82-83	22.899424787051245	27.103099185676598	26.97767648563258	23.01979954163958
84-85	23.02928535129805	27.157944037874763	26.903641224403202	22.90912938642398
86-87	22.932527881404923	27.14006997525352	26.880925449523613	23.046476693817937
88-89	23.00284725480055	27.050918426181465	26.891578707800416	23.054655611217566
90-91	22.955553703493702	27.13455214184122	26.929046144121077	22.980848010544
92-93	23.069715097481218	27.073810830631288	26.913339267841575	22.94313480404592
94-95	22.928172248485215	27.08084360726259	26.991704477457272	22.999279666794923
96-97	22.962609012749446	27.18670420740534	27.104693392532774	22.745993387312435
98-99	24.186147536515794	29.054706378845864	27.192206936307343	19.566939148331002
100	23.202032316900954	29.896139385071457	21.02348098498253	25.87834731304505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1225.0
1	1194.5
2	1929.5
3	2846.0
4	4199.5
5	7461.5
6	10044.5
7	11627.0
8	12459.5
9	11981.0
10	10812.5
11	9680.5
12	10624.5
13	14362.5
14	23208.5
15	35548.0
16	45605.5
17	51542.5
18	52524.5
19	48654.0
20	42353.0
21	35866.5
22	31446.5
23	30999.5
24	35582.5
25	43809.0
26	54533.0
27	66956.5
28	78762.5
29	92918.0
30	106183.5
31	118071.5
32	133216.5
33	147192.0
34	161062.0
35	175207.5
36	189572.5
37	203962.5
38	213322.5
39	218054.0
40	226008.5
41	235462.5
42	244275.0
43	256792.5
44	270894.5
45	284565.5
46	307103.0
47	339873.0
48	398031.0
49	422497.5
50	399904.0
51	400479.0
52	393642.0
53	352192.5
54	307140.0
55	275992.5
56	250991.5
57	227388.0
58	200721.5
59	174407.5
60	147062.5
61	119509.5
62	95043.5
63	73551.5
64	54959.5
65	40890.5
66	31212.0
67	24087.5
68	18262.0
69	13232.0
70	9547.0
71	6836.0
72	4732.5
73	3181.5
74	2108.0
75	1387.0
76	908.0
77	544.0
78	334.0
79	221.5
80	148.5
81	100.0
82	74.5
83	52.0
84	33.5
85	25.0
86	19.5
87	15.0
88	11.0
89	7.0
90	3.5
91	2.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001244386526047459
4	0.01127890766162165
5	0.011133287961765033
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.6476890903427262E-5
22-23	0.0
24-25	0.005991969569004581
26-27	0.04526295431845939
28-29	0.08124147039109551
30-31	0.0733149651111937
32-33	0.03336860675356386
34-35	0.0028898416393477633
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.3007389353365099E-4
56-57	3.590363552153291E-4
58-59	7.441356529529908E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.4798686073992442E-4
72-73	0.01273204230092406
74-75	0.028610843434259653
76-77	0.0025037526684843683
78-79	0.0
80-81	0.0
82-83	0.0077064592245924615
84-85	0.045467310694434576
86-87	0.037998167545044294
88-89	0.009466255274490357
90-91	3.6433990399097025E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	98.0
20-21	408.0
22-23	992.0
24-25	2470.0
26-27	3006.0
28-29	7521.0
30-31	18200.0
32-33	22521.0
34-35	30632.0
36-37	43262.0
38-39	49566.0
40-41	53574.0
42-43	62750.0
44-45	74399.0
46-47	71817.0
48-49	65314.0
50-51	55583.0
52-53	55382.0
54-55	82107.0
56-57	110157.0
58-59	99564.0
60-61	107890.0
62-63	116734.0
64-65	116681.0
66-67	128653.0
68-69	77853.0
70-71	58885.0
72-73	55758.0
74-75	57385.0
76-77	55605.0
78-79	59107.0
80-81	60242.0
82-83	57848.0
84-85	58263.0
86-87	61074.0
88-89	65695.0
90-91	69082.0
92-93	72646.0
94-95	82025.0
96-97	230417.0
98-99	428675.0
100-101	4624082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.38506069419971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19277304049145	71.77574186690175
2	7.6267015167622425	12.4139913167648
3	2.1454543833970385	5.238238056282143
4	0.8433042302917221	2.7452946386386867
5	0.4374556137035397	1.780117583614048
6	0.23553178584358128	1.1501261211775866
7	0.140173374129112	0.7985612992845978
8	0.09622381741974942	0.6264944976747117
9	0.06037795210816285	0.4422476967222885
>10	0.2175174679531561	2.7180932310623405
>50	0.003579255753294771	0.19175756863360954
>100	8.762923641001396E-4	0.10490657896237786
>500	3.126978280222043E-5	0.014429544281005372
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3238154532419776E-5	0.0
2	0.0	1.3238154532419776E-5	0.0	2.6476309064839553E-5	0.0
3	0.0	1.3238154532419776E-5	0.0	3.971446359725933E-5	0.0
4	0.0	5.2952618129679105E-5	0.0	5.2952618129679105E-5	0.0
5	0.0	6.619077266209888E-5	0.0	6.619077266209888E-5	0.0
6	1.5885785438903732E-4	6.619077266209888E-5	0.0	6.619077266209888E-5	0.0
7	1.5885785438903732E-4	9.266708172693843E-5	0.0	1.9857231798629666E-4	0.0
8	1.5885785438903732E-4	1.0590523625935821E-4	0.0	2.3828678158355597E-4	0.0
9	1.5885785438903732E-4	2.780012451808153E-4	0.0	2.515249361159758E-4	0.0
10-11	1.5885785438903732E-4	2.780012451808153E-4	0.0	2.846203224470252E-4	0.0
12-13	1.5885785438903732E-4	2.780012451808153E-4	0.0	3.375729405767043E-4	0.0
14-15	1.5885785438903732E-4	4.037637132388032E-4	0.0	3.905255587063834E-4	0.0
16-17	3.2433478604428453E-4	4.2362094503743284E-4	0.0	4.699544859009021E-4	0.0
18-19	3.309538633104944E-4	4.500972541022724E-4	0.0	6.221932630237296E-4	6.619077266209888E-6
20-21	3.309538633104944E-4	4.964307949657416E-4	0.0	8.207655810100261E-4	1.3238154532419776E-5
22-23	3.309538633104944E-4	6.023360312250998E-4	0.0	0.0012708628351122985	1.3238154532419776E-5
24-25	3.309538633104944E-4	6.619077266209888E-4	0.0	0.0028329650699378322	1.3238154532419776E-5
26-27	3.375729405767043E-4	6.751458811534086E-4	0.0	0.006857364047793444	1.3238154532419776E-5
28-29	3.441920178429142E-4	6.883840356858284E-4	0.0	0.019393896389994975	1.3238154532419776E-5
30-31	3.508110951091241E-4	8.737181991397053E-4	0.0	0.02196871744655062	1.3238154532419776E-5
32-33	3.7066832690775374E-4	8.737181991397053E-4	0.0	0.026919787241675618	1.9857231798629665E-5
34-35	3.8390648144017353E-4	0.001012718821730113	0.0	0.03276443246773895	2.6476309064839553E-5
36-37	3.971446359725933E-4	0.0011517194443205207	0.0	0.04161413877266157	2.6476309064839553E-5
38-39	3.971446359725933E-4	0.0011781957533853601	0.0	0.06415209686410624	2.6476309064839553E-5
40-41	3.971446359725933E-4	0.0012708628351122985	0.0	0.08770939285454724	2.6476309064839553E-5
42-43	4.037637132388032E-4	0.0012841009896447184	0.0	0.10172197942711356	2.6476309064839553E-5
44-45	4.2362094503743284E-4	0.0015091496166958545	0.0	0.12067239764027247	2.6476309064839553E-5
46-47	4.3685909956985263E-4	0.0016415311620200524	0.0	0.14025162819372133	2.6476309064839553E-5
48-49	4.500972541022724E-4	0.0016481502392862624	0.0	0.15719646599521864	2.6476309064839553E-5
50-51	4.964307949657416E-4	0.00179376993914288	0.0	0.17532611862736752	2.6476309064839553E-5
52-53	5.162880267643713E-4	0.0018003890164090897	0.0	0.22146770625011666	2.6476309064839553E-5
54-55	5.295261812967911E-4	0.0018070080936752994	0.0	0.2791728218569345	2.6476309064839553E-5
56-57	5.427643358292108E-4	0.001840103480006349	0.0	0.33986976038807915	2.6476309064839553E-5
58-59	5.427643358292108E-4	0.001840103480006349	0.0	0.38542225013413556	3.309538633104944E-5
60-61	5.692406448940504E-4	0.0019129133299346577	0.0	0.41649219882172483	3.971446359725933E-5
62-63	6.883840356858284E-4	0.001952627793531917	0.0	0.4407114025387868	3.971446359725933E-5
64-65	6.883840356858284E-4	0.002197533652381683	0.0	0.44785338690902726	3.971446359725933E-5
66-67	7.14860344750668E-4	0.0022107718069141026	0.0	0.45392969983940795	3.971446359725933E-5
68-69	7.14860344750668E-4	0.002290200734108621	0.0	0.4602906330922356	3.971446359725933E-5
70-71	7.545748083479273E-4	0.002329915197705881	0.0	0.4655130850552752	3.971446359725933E-5
72-73	8.538609673410756E-4	0.0023431533522383008	0.0	0.46932567356061217	3.971446359725933E-5
74-75	8.604800446072855E-4	0.0024225822794328193	0.0	0.4723241155622052	3.971446359725933E-5
76-77	8.604800446072855E-4	0.0025814401338218563	0.0	0.4754417009545901	3.971446359725933E-5
78-79	8.604800446072855E-4	0.002641011829217745	0.0	0.47736785243905716	3.971446359725933E-5
80-81	8.670991218734953E-4	0.0026476309064839554	0.0	0.4778576641567567	3.971446359725933E-5
82-83	8.737181991397053E-4	0.0026476309064839554	0.0	0.47823495156093065	3.971446359725933E-5
84-85	8.737181991397053E-4	0.0026476309064839554	0.0	0.47842690480165073	3.971446359725933E-5
86-87	8.737181991397053E-4	0.0026873453700812146	0.0	0.4785592863469749	3.971446359725933E-5
88	8.737181991397053E-4	0.0029256321516647706	0.0	0.47857252450150733	3.971446359725933E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	20075	0.0	56.76019	1
TTATACA	23675	0.0	48.532997	2
TATACAC	24530	0.0	47.36653	3
GGTATCA	12065	0.0	47.290638	1
GTATCAA	22135	0.0	36.36487	1
TACACAT	35045	0.0	33.113487	5
ATACACA	36880	0.0	31.954985	4
ATCAACG	27315	0.0	29.149174	3
TCAACGC	27355	0.0	29.12116	4
TATCAAC	27515	0.0	29.073221	2
CAACGCA	27615	0.0	28.812687	5
AACGCAG	27935	0.0	28.55398	6
ACACATC	41340	0.0	27.786812	6
ACATCTC	42690	0.0	27.005417	8
CACATCT	42830	0.0	26.664946	7
CGCAGAG	32345	0.0	24.558102	8
ACGCAGA	32825	0.0	24.224302	7
CATCTCC	47745	0.0	23.989595	9
GCAGAGT	36555	0.0	21.616123	9
GTGGTAT	6100	0.0	21.459156	1
>>END_MODULE