FastQCFastQC Report
Fri 27 May 2016
SRR2138642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7737993
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114800.052.393831
GTATCAA205850.037.585631
CTTATAC91000.034.884441
TCAACGC249600.030.4996594
ATCAACG251100.030.3688433
CAACGCA254100.029.9893675
TATCAAC257750.029.7515282
AACGCAG260850.029.1774736
TATACAC123850.028.2605743
TTATACA127500.025.9395282
ACGCAGA299750.025.419557
CGCAGAG302750.025.1393688
GTGGTAT53300.024.5951331
TGGTATC51250.024.0742932
GCAGAGT348700.021.6915139
ACACATC148400.021.5883186
TACACAT161650.021.5697295
GAGTACT206550.020.83972412-13
GTACATG250350.020.3464761
TACATGG241750.020.2373922
Produced by FastQC (version 0.11.5)