##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7737993
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7880436180286	33.0	33.0	33.0	33.0	33.0
2	32.78635235260616	33.0	33.0	33.0	33.0	33.0
3	32.78602229286069	33.0	33.0	33.0	33.0	33.0
4	36.72770174385012	37.0	37.0	37.0	37.0	37.0
5	36.74114851228219	37.0	37.0	37.0	37.0	37.0
6	36.750687290619155	37.0	37.0	37.0	37.0	37.0
7	36.737618527181404	37.0	37.0	37.0	37.0	37.0
8	36.73081870195541	37.0	37.0	37.0	37.0	37.0
9	36.72874490840196	37.0	37.0	37.0	37.0	37.0
10-11	36.70051207076563	37.0	37.0	37.0	37.0	37.0
12-13	36.67937810230637	37.0	37.0	37.0	37.0	37.0
14-15	38.92856067458319	40.0	40.0	40.0	37.0	40.0
16-17	38.87838429163738	40.0	40.0	40.0	37.0	40.0
18-19	38.90672768765751	40.0	40.0	40.0	37.0	40.0
20-21	38.94396770288035	40.0	40.0	40.0	37.0	40.0
22-23	38.969196004326676	40.0	40.0	40.0	37.0	40.0
24-25	38.97642458014557	40.0	40.0	40.0	37.0	40.0
26-27	38.88516892728173	40.0	40.0	40.0	37.0	40.0
28-29	38.81489295949326	40.0	38.5	40.0	37.0	40.0
30-31	38.759388922811404	40.0	37.0	40.0	37.0	40.0
32-33	38.43761596431047	40.0	37.0	40.0	37.0	40.0
34-35	38.5718284516651	40.0	37.0	40.0	37.0	40.0
36-37	38.545431497524376	40.0	37.0	40.0	37.0	40.0
38-39	38.57192625057566	40.0	37.0	40.0	37.0	40.0
40-41	38.546209908612425	40.0	37.0	40.0	37.0	40.0
42-43	38.250541732399064	40.0	37.0	40.0	37.0	40.0
44-45	38.155765830951225	40.0	37.0	40.0	37.0	40.0
46-47	38.185806651316135	40.0	37.0	40.0	37.0	40.0
48-49	38.060966861377686	40.0	37.0	40.0	37.0	40.0
50-51	38.15502449593124	40.0	37.0	40.0	37.0	40.0
52-53	38.152181212135524	40.0	37.0	40.0	37.0	40.0
54-55	38.0897600607205	40.0	37.0	40.0	37.0	40.0
56-57	37.967715419962566	40.0	37.0	40.0	35.0	40.0
58-59	37.84772782240043	40.0	37.0	40.0	33.0	40.0
60-61	37.603656323551306	40.0	37.0	40.0	33.0	40.0
62-63	37.41800217695165	37.0	37.0	40.0	33.0	40.0
64-65	37.25853041925181	37.0	37.0	40.0	33.0	40.0
66-67	37.02797630251648	37.0	37.0	40.0	33.0	40.0
68-69	36.76138379352976	37.0	37.0	40.0	33.0	40.0
70-71	36.313003500780255	37.0	37.0	40.0	33.0	40.0
72-73	36.206605294904435	37.0	37.0	37.0	33.0	40.0
74-75	35.974976676230604	37.0	37.0	37.0	33.0	40.0
76-77	33.939673896207765	35.0	33.0	37.0	27.0	37.0
78-79	35.34103467784265	37.0	33.0	37.0	33.0	37.0
80-81	35.64276201383308	37.0	37.0	37.0	33.0	37.0
82-83	35.64219567104548	37.0	37.0	37.0	33.0	37.0
84-85	35.53485862671994	37.0	37.0	37.0	33.0	37.0
86-87	35.10656224380436	37.0	33.0	37.0	33.0	37.0
88-89	35.0339048732371	37.0	33.0	37.0	33.0	37.0
90-91	35.20321727585977	37.0	33.0	37.0	33.0	37.0
92-93	35.19890554233223	37.0	33.0	37.0	33.0	37.0
94-95	35.171385438036424	37.0	33.0	37.0	33.0	37.0
96-97	35.130501561639136	37.0	33.0	37.0	33.0	37.0
98-99	35.09269492591689	37.0	33.0	37.0	33.0	37.0
100	35.037837011156086	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	23.0
21	120.0
22	530.0
23	1564.0
24	3868.0
25	8090.0
26	14850.0
27	24713.0
28	37838.0
29	53960.0
30	73329.0
31	99429.0
32	134549.0
33	183810.0
34	263781.0
35	409895.0
36	805092.0
37	2281008.0
38	3225264.0
39	116275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43878134808341	20.086397079966343	14.674968044039327	23.79985352791092
2	14.406216185514772	20.67572302016815	42.12845630643501	22.789604487882066
3	18.094796606776548	25.84290064491716	30.739388616513562	25.32291413179272
4	12.340552359339759	18.334102139806628	38.46333400585051	30.8620114950031
5	13.387422414536273	37.478307477220504	35.41898638419782	13.71528372404539
6	29.71628431299951	37.71722202385037	18.705147445855793	13.86134621729433
7	26.316901553154675	32.90534121703134	22.444773470330098	18.332983759483888
8	24.442216476546307	36.44331288487855	21.149502202961415	17.96496843561373
9	25.334954425520934	17.005947149344795	20.997356808154258	36.66174161698001
10-11	24.0408720969378	26.834180387601798	28.591703300842997	20.533244214617408
12-13	24.998058282037732	24.316686510313463	28.699192413329914	21.98606279431889
14-15	22.212575017837313	25.782473569050783	26.461305922608098	25.543645490503803
16-17	21.6722992641632	29.13087411684141	27.075063262528154	22.121763356467238
18-19	21.608632884521864	27.84360233977984	29.068519446838476	21.47924532885982
20-21	22.394433890953135	26.904846307665885	29.110426193186743	21.590293608194237
22-23	21.994363634131226	27.126371045028243	29.000737341602417	21.878527979238115
24-25	21.85271107932082	27.30669089462615	29.16556324608549	21.67503477996754
26-27	21.76635626156823	27.395621124450635	29.124719718523494	21.713302895457637
28-29	21.42732518938142	27.18983162046119	29.20558628497263	22.177256905184763
30-31	21.788816802144698	27.0207387929278	29.274537561001406	21.91590684392609
32-33	21.715276241065855	27.211133626352424	29.08355250478616	21.990037627795562
34-35	21.74086519854654	27.602971723882508	29.206918683746352	21.4492443938246
36-37	21.86102260993427	27.394980750436133	28.800506224046334	21.943490415583266
38-39	21.786814087088324	27.327160526654954	29.01279541976142	21.873229966495302
40-41	22.005588101891853	27.21910040382582	28.97137874275896	21.803932751523362
42-43	22.1516824133269	27.208806431071636	28.80803963718206	21.831471518419406
44-45	22.02212649727308	27.511896647070404	28.525990922730653	21.939985932925865
46-47	22.288482519742022	27.292499582675738	28.047881848656214	22.371136048926022
48-49	22.16695611017926	27.431975091376742	28.11402289882935	22.287045899614647
50-51	21.990979316359464	27.705306651914153	28.10240006200784	22.201313969718544
52-53	22.132646980217825	27.718022523935765	28.161442105140843	21.98788839070557
54-55	22.105243590102415	27.69479152785535	27.89747867823359	22.30248620380864
56-57	22.319888147641073	27.895581730781377	27.62959838177266	22.154931739804894
58-59	21.971355268722455	28.12194165702075	28.014595012250705	21.892108062006095
60-61	22.36490684525918	28.281219000052864	27.49050966882666	21.863364485861293
62-63	22.040822024142997	28.456972789874346	27.48663195491281	22.015573231069848
64-65	22.09267619323442	28.28803406116404	27.507042093958688	22.112247651642853
66-67	22.18331206881384	28.137446008065524	27.448186489530247	22.231055433590388
68-69	22.197511992444834	27.97261334469503	27.560178628083726	22.269696034776405
70-71	22.23250291452394	27.673804746181517	27.610603156207418	22.48308918308712
72-73	22.26170242492394	27.728923005089246	27.584423503885695	22.424951066101116
74-75	22.153732866140164	27.8744108431597	27.538684117133737	22.433172173566398
76-77	22.29451713702338	27.796909463206237	27.41581537407661	22.492758025693764
78-79	22.30164922271503	27.869237470180785	27.363751213549055	22.46536209355513
80-81	22.252500210952135	27.88894669963436	27.389628440894015	22.46892464851949
82-83	22.300075727285034	27.826361512824484	27.505753357708873	22.367809402181614
84-85	22.43190532616388	27.79819852995937	27.42940486901439	22.340491274862355
86-87	22.294877226736283	27.836236669514413	27.488744282224847	22.38014182152445
88-89	22.365549371420606	27.86062661790477	27.439293890223794	22.334530120450832
90-91	22.308533619166184	27.796437940107072	27.539523999678718	22.355504441048033
92-93	22.316402253108002	27.803015501047785	27.550553186577158	22.330029059267055
94-95	22.140643313126674	27.959417934238278	27.547983281993915	22.351955470641133
96-97	22.27759511006142	27.983342761010338	27.662118045590855	22.076944083337388
98-99	23.364304454147288	29.912833912552323	27.848123079314778	18.874738553985612
100	22.328309531969406	30.942259225387165	21.70560813121267	25.023823111430755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2032.0
1	1784.5
2	2140.0
3	2965.5
4	4125.5
5	6654.0
6	8739.0
7	10149.5
8	11304.5
9	11492.0
10	10782.5
11	10213.5
12	11487.0
13	15205.0
14	23450.5
15	34645.5
16	43907.0
17	49466.0
18	50639.5
19	47903.0
20	43007.5
21	37524.0
22	34003.5
23	34640.0
24	39582.5
25	48561.5
26	60826.5
27	74769.5
28	89546.5
29	105877.5
30	121857.5
31	137604.0
32	155176.5
33	172437.0
34	190204.5
35	207656.5
36	225524.5
37	242471.5
38	253233.0
39	262905.0
40	275966.5
41	287321.0
42	294985.5
43	302586.0
44	309843.0
45	319712.0
46	334090.0
47	345930.0
48	344215.5
49	335034.5
50	325954.5
51	308400.5
52	287385.0
53	268651.0
54	251147.5
55	233604.5
56	214100.0
57	192453.0
58	170961.0
59	149416.0
60	124834.5
61	100496.0
62	78847.5
63	60640.0
64	45405.0
65	33413.0
66	25047.0
67	19089.0
68	14490.0
69	10525.5
70	7497.5
71	5379.5
72	3729.0
73	2465.0
74	1607.5
75	1045.0
76	679.0
77	406.0
78	256.0
79	178.5
80	128.0
81	89.0
82	67.0
83	49.0
84	28.0
85	16.0
86	12.0
87	8.0
88	4.0
89	3.0
90	2.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012923247669001509
4	0.011527536920749348
5	0.011372457948721328
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.938527859973154E-5
22-23	0.0
24-25	0.005726578375237861
26-27	0.04580723204708867
28-29	0.08113711765354276
30-31	0.07394666476193079
32-33	0.034080396121661305
34-35	0.003077611573016725
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.8102007317666834E-4
56-57	4.631023224581471E-4
58-59	2.1223136019715443E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.2425114693249096E-4
72-73	0.012764063149411272
74-75	0.028758320225528412
76-77	0.0029166856774350807
78-79	0.0
80-81	0.0
82-83	0.007529759233789288
84-85	0.04557532492514124
86-87	0.03854921988363169
88-89	0.009516181217767969
90-91	8.960780050566495E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	432.0
22-23	1010.0
24-25	2273.0
26-27	3287.0
28-29	6399.0
30-31	14890.0
32-33	19183.0
34-35	26558.0
36-37	37727.0
38-39	43555.0
40-41	47397.0
42-43	55784.0
44-45	67137.0
46-47	66006.0
48-49	56968.0
50-51	48945.0
52-53	46458.0
54-55	52664.0
56-57	59104.0
58-59	58366.0
60-61	59948.0
62-63	63103.0
64-65	66357.0
66-67	72393.0
68-69	59298.0
70-71	53976.0
72-73	51513.0
74-75	53020.0
76-77	52043.0
78-79	54921.0
80-81	55543.0
82-83	54340.0
84-85	55044.0
86-87	57286.0
88-89	61020.0
90-91	64627.0
92-93	68766.0
94-95	84665.0
96-97	248176.0
98-99	476393.0
100-101	5211326.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.59789178662317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.98491207016549	77.02518765810827
2	7.1000263849571335	12.1549458036346
3	1.644955459342971	4.224141582079639
4	0.6039020222297342	2.0677095979417475
5	0.2585042914267446	1.106371118196209
6	0.1343031272297572	0.6897638730730704
7	0.08145001242948147	0.48803645449705063
8	0.05068655460238537	0.34709297727134
9	0.03140847081836046	0.2419648997663997
>10	0.1073739605608867	1.3810840890187681
>50	0.0019150300975523643	0.10572781661402791
>100	4.4452841613473303E-4	0.06976437099857766
>500	7.085281579372219E-5	0.03551981089631591
>1k	4.7234907575617045E-5	0.06268994790399673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.876974300700453E-5	0.0	2.584649533800302E-5	0.0
5	0.0	3.876974300700453E-5	0.0	2.584649533800302E-5	0.0
6	0.0	3.876974300700453E-5	0.0	2.584649533800302E-5	0.0
7	0.0	3.876974300700453E-5	0.0	2.584649533800302E-5	1.292324766900151E-5
8	0.0	5.169299067600604E-5	0.0	2.584649533800302E-5	1.292324766900151E-5
9	0.0	1.292324766900151E-4	0.0	3.876974300700453E-5	1.292324766900151E-5
10-11	0.0	1.292324766900151E-4	0.0	3.876974300700453E-5	1.292324766900151E-5
12-13	0.0	1.292324766900151E-4	0.0	5.169299067600604E-5	1.292324766900151E-5
14-15	0.0	2.584649533800302E-4	0.0	5.169299067600604E-5	1.292324766900151E-5
16-17	0.0	2.713882010490317E-4	0.0	8.400110984850982E-5	1.292324766900151E-5
18-19	0.0	2.843114487180332E-4	0.0	9.692435751751133E-5	1.292324766900151E-5
20-21	0.0	2.9723469638703474E-4	0.0	1.163092290210136E-4	1.292324766900151E-5
22-23	0.0	3.4892768706304075E-4	0.0	1.2277085285551437E-4	1.292324766900151E-5
24-25	0.0	4.135439254080483E-4	0.0	1.4861734819351734E-4	1.292324766900151E-5
26-27	0.0	4.264671730770498E-4	0.0	1.873870912005219E-4	1.292324766900151E-5
28-29	0.0	4.264671730770498E-4	0.0	4.135439254080483E-4	1.292324766900151E-5
30-31	0.0	4.652369160840544E-4	0.0	0.0015378664726111797	1.292324766900151E-5
32-33	0.0	4.846217875875566E-4	0.0	0.0039351289152109595	1.292324766900151E-5
34-35	0.0	5.621612736015657E-4	0.0	0.006571471439687268	1.292324766900151E-5
36-37	0.0	6.397007596155746E-4	0.0	0.010571216593243235	1.292324766900151E-5
38-39	0.0	6.59085631119077E-4	0.0	0.02293230298864318	1.292324766900151E-5
40-41	1.292324766900151E-5	8.529343461540996E-4	0.0	0.0359266285198242	1.292324766900151E-5
42-43	1.292324766900151E-5	8.723192176576019E-4	0.0	0.04359657601137659	1.292324766900151E-5
44-45	1.292324766900151E-5	9.563203275061117E-4	0.0	0.05345701398282474	1.292324766900151E-5
46-47	1.292324766900151E-5	0.0010080133181821177	0.0	0.0641380781812545	1.292324766900151E-5
48-49	1.292324766900151E-5	0.0010209365658511193	0.0	0.07349450949361158	1.292324766900151E-5
50-51	1.292324766900151E-5	0.001163092290210136	0.0	0.08415618882053783	1.292324766900151E-5
52-53	1.292324766900151E-5	0.001163092290210136	0.0	0.11164393661250405	1.292324766900151E-5
54-55	1.292324766900151E-5	0.001163092290210136	0.0	0.14873365742253838	1.292324766900151E-5
56-57	1.292324766900151E-5	0.001163092290210136	0.0	0.18791048273111643	1.292324766900151E-5
58-59	1.292324766900151E-5	0.001163092290210136	0.0	0.22462542935874974	1.292324766900151E-5
60-61	1.292324766900151E-5	0.0012277085285551433	0.0	0.23787821984331078	1.292324766900151E-5
62-63	1.292324766900151E-5	0.0012600166477276472	0.0	0.256591082468025	1.292324766900151E-5
64-65	1.292324766900151E-5	0.0014086339959211646	0.0	0.2621868487087027	1.292324766900151E-5
66-67	1.292324766900151E-5	0.0014344804912591676	0.0	0.2669296806032262	1.292324766900151E-5
68-69	1.292324766900151E-5	0.001447403738928169	0.0	0.2714140475443697	1.292324766900151E-5
70-71	1.292324766900151E-5	0.0014603269865971706	0.0	0.27561410303679523	1.292324766900151E-5
72-73	1.292324766900151E-5	0.0014603269865971706	0.0	0.27896768580690107	1.292324766900151E-5
74-75	1.292324766900151E-5	0.0014926351057696742	0.0	0.28178495379874346	1.292324766900151E-5
76-77	1.292324766900151E-5	0.0015895594632871857	0.0	0.28475730076261374	1.292324766900151E-5
78-79	1.292324766900151E-5	0.0016218675824596893	0.0	0.286760404151309	1.292324766900151E-5
80-81	1.292324766900151E-5	0.0016283292062941903	0.0	0.28741948978242804	1.292324766900151E-5
82-83	1.292324766900151E-5	0.0016283292062941903	0.0	0.28778780234099466	1.292324766900151E-5
84-85	1.292324766900151E-5	0.0016283292062941903	0.0	0.2879299580653536	1.292324766900151E-5
86-87	1.292324766900151E-5	0.0016412524539631919	0.0	0.2880074975513677	1.292324766900151E-5
88	1.292324766900151E-5	0.0017317151876462024	0.0	0.28802042079903667	1.292324766900151E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11480	0.0	52.39383	1
GTATCAA	20585	0.0	37.58563	1
CTTATAC	9100	0.0	34.88444	1
TCAACGC	24960	0.0	30.499659	4
ATCAACG	25110	0.0	30.368843	3
CAACGCA	25410	0.0	29.989367	5
TATCAAC	25775	0.0	29.751528	2
AACGCAG	26085	0.0	29.177473	6
TATACAC	12385	0.0	28.260574	3
TTATACA	12750	0.0	25.939528	2
ACGCAGA	29975	0.0	25.41955	7
CGCAGAG	30275	0.0	25.139368	8
GTGGTAT	5330	0.0	24.595133	1
TGGTATC	5125	0.0	24.074293	2
GCAGAGT	34870	0.0	21.691513	9
ACACATC	14840	0.0	21.588318	6
TACACAT	16165	0.0	21.569729	5
GAGTACT	20655	0.0	20.839724	12-13
GTACATG	25035	0.0	20.346476	1
TACATGG	24175	0.0	20.237392	2
>>END_MODULE