FastQCFastQC Report
Fri 27 May 2016
SRR2138641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942411
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170650.052.68361
GTATCAA322500.038.2200661
TCAACGC391950.030.9223484
ATCAACG392250.030.8335423
TATCAAC399400.030.5713922
CAACGCA398850.030.3622935
AACGCAG408350.029.6941266
CTTATAC72900.026.1119141
ACGCAGA474350.025.481237
CGCAGAG477850.025.3304738
GTGGTAT83250.025.028531
TGGTATC83400.023.2871042
GCAGAGT541400.022.277999
GAGTACT324750.021.20981412-13
TACATGG367400.020.3278882
TATACAC114300.020.2924423
GTACATG379550.020.0612161
CAGAGTA527850.019.80484410-11
ACATGGG378550.019.1515393
GTACTTT362950.018.98384914-15
Produced by FastQC (version 0.11.5)