FastQCFastQC Report
Fri 27 May 2016
SRR2138640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7694134
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123850.052.6131
GTATCAA228900.037.8946381
ATCAACG282900.030.1537653
TCAACGC282700.030.1446324
TATCAAC286600.029.9265632
CAACGCA288400.029.5881615
AACGCAG291750.029.3160136
ACGCAGA336800.025.3822027
CGCAGAG340500.025.1063888
GTGGTAT55350.023.8045561
TGGTATC55750.022.1945272
GCAGAGT386400.021.960159
GAGTACT224600.020.44070412-13
GTACATG276200.019.9072111
TACATGG271100.019.87672
CAGAGTA376900.019.61265410-11
ACATGGG278750.018.9676073
AGAGTAC352050.018.952510-11
GTACTTT245200.018.58604414-15
GGACCGA15450.017.4875036
Produced by FastQC (version 0.11.5)