##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7694134
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82819119084747	33.0	33.0	33.0	33.0	33.0
2	32.82542050866283	33.0	33.0	33.0	33.0	33.0
3	32.82144384280284	33.0	33.0	33.0	33.0	33.0
4	36.77538329849727	37.0	37.0	37.0	37.0	37.0
5	36.78891243640935	37.0	37.0	37.0	37.0	37.0
6	36.79553891315124	37.0	37.0	37.0	37.0	37.0
7	36.78187135810216	37.0	37.0	37.0	37.0	37.0
8	36.78117680300343	37.0	37.0	37.0	37.0	37.0
9	36.77776953195772	37.0	37.0	37.0	37.0	37.0
10-11	36.75664493236016	37.0	37.0	37.0	37.0	37.0
12-13	36.73981457822284	37.0	37.0	37.0	37.0	37.0
14-15	39.08017737928661	40.0	40.0	40.0	37.0	40.0
16-17	39.034578160453144	40.0	40.0	40.0	37.0	40.0
18-19	39.06903635938755	40.0	40.0	40.0	37.0	40.0
20-21	39.082987884513	40.0	40.0	40.0	37.0	40.0
22-23	39.11544960647342	40.0	40.0	40.0	37.0	40.0
24-25	39.11715470042344	40.0	40.0	40.0	37.0	40.0
26-27	39.03139153575963	40.0	40.0	40.0	37.0	40.0
28-29	38.96187172276176	40.0	40.0	40.0	37.0	40.0
30-31	38.88962057112032	40.0	40.0	40.0	37.0	40.0
32-33	38.85931139575298	40.0	40.0	40.0	37.0	40.0
34-35	38.84022553312151	40.0	40.0	40.0	37.0	40.0
36-37	38.80698011852343	40.0	37.0	40.0	37.0	40.0
38-39	38.763245399641065	40.0	37.0	40.0	37.0	40.0
40-41	38.7165347884195	40.0	37.0	40.0	37.0	40.0
42-43	38.64792010465423	40.0	37.0	40.0	37.0	40.0
44-45	38.56184087558628	40.0	37.0	40.0	37.0	40.0
46-47	38.47833452027241	40.0	37.0	40.0	37.0	40.0
48-49	38.429989617721745	40.0	37.0	40.0	37.0	40.0
50-51	38.379785887399265	40.0	37.0	40.0	37.0	40.0
52-53	38.306892772898365	40.0	37.0	40.0	37.0	40.0
54-55	38.23653108584651	40.0	37.0	40.0	37.0	40.0
56-57	38.130654529229126	40.0	37.0	40.0	37.0	40.0
58-59	38.007636521204546	40.0	37.0	40.0	37.0	40.0
60-61	37.83295417177194	40.0	37.0	40.0	33.0	40.0
62-63	37.63918651332771	38.5	37.0	40.0	33.0	40.0
64-65	37.40819591959173	37.0	37.0	40.0	33.0	40.0
66-67	37.1706472109435	37.0	37.0	40.0	33.0	40.0
68-69	36.92463250411771	37.0	37.0	40.0	33.0	40.0
70-71	36.66071639974032	37.0	37.0	40.0	33.0	40.0
72-73	36.41709285805683	37.0	37.0	37.0	33.0	40.0
74-75	36.17930513109947	37.0	37.0	37.0	33.0	40.0
76-77	34.55988606786028	35.0	33.0	37.0	30.0	37.0
78-79	35.80004369770876	37.0	37.0	37.0	33.0	37.0
80-81	35.91476782168485	37.0	37.0	37.0	33.0	37.0
82-83	35.85398066161749	37.0	37.0	37.0	33.0	37.0
84-85	35.736264064637396	37.0	37.0	37.0	33.0	37.0
86-87	35.62823453968272	37.0	37.0	37.0	33.0	37.0
88-89	35.558213193996195	37.0	37.0	37.0	33.0	37.0
90-91	35.497802296813006	37.0	35.0	37.0	33.0	37.0
92-93	35.428951402833064	37.0	33.0	37.0	33.0	37.0
94-95	35.38696965108376	37.0	33.0	37.0	33.0	37.0
96-97	35.35133162774379	37.0	33.0	37.0	33.0	37.0
98-99	35.30550563504311	37.0	33.0	37.0	33.0	37.0
100	35.251416949266876	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	7.0
20	34.0
21	119.0
22	418.0
23	1255.0
24	3073.0
25	6367.0
26	11754.0
27	18963.0
28	28984.0
29	41821.0
30	58052.0
31	79459.0
32	107536.0
33	147515.0
34	214191.0
35	333467.0
36	664325.0
37	2169987.0
38	3635881.0
39	170923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27809133555511	19.805282829750563	14.546211438480276	23.370414396214052
2	14.877242845003739	20.498720193851575	41.614481889709744	23.00955507143494
3	18.374977060034865	25.709020384206077	30.228635419098214	25.687367136660843
4	12.699480426702106	17.744145636504587	38.48432571157313	31.07204822522018
5	13.58552349357966	37.508235037190914	35.054557933844116	13.851683535385312
6	30.25537770142523	37.27189275256874	18.54431162029562	13.928417925710407
7	26.92769932622688	32.75399580433559	22.46093224538749	17.85737262405004
8	24.919259908816237	36.052431118619864	20.72728662215743	18.301022350406473
9	25.91342000456712	16.91049530856823	21.10994182190508	36.06614286495957
10-11	24.643302890657075	26.610087192410113	28.183688792486432	20.56292112444638
12-13	25.47999235261465	24.251049468471628	28.445388193835484	21.82356998507824
14-15	22.581620139551777	25.4444571308777	26.57317422423201	25.400748505338516
16-17	21.796949468257246	28.639785842045384	27.162075679992054	22.401189009705316
18-19	21.845129029465824	27.632485995175028	29.196593144855548	21.3257918305036
20-21	22.80457293178944	26.678054372450116	29.154734161947605	21.362638533812845
22-23	22.535036709394912	26.583036398481752	29.0002497573753	21.88167713474804
24-25	22.22322623323901	26.758847446203355	29.09644729184858	21.92147902870905
26-27	22.117051400727266	26.945103212373045	28.994900862980927	21.94294452391876
28-29	21.99703061450127	27.05227427638733	29.033484234217365	21.91721087489404
30-31	22.339894737171647	26.860439876944653	28.94556898487688	21.854096401006817
32-33	22.089523790350043	27.045225203059974	28.849387156357654	22.01586385023233
34-35	22.18005543835234	27.090311376166543	28.87363913821256	21.85599404726856
36-37	22.244745004439217	27.050913457499643	28.761015271237	21.94332626682414
38-39	22.15002766422399	27.14229893018399	28.770414804141275	21.937258601450747
40-41	22.381892819462603	27.068315469108217	28.588199650581664	21.96159206084751
42-43	22.2546696301292	27.16455209301617	28.427022443902374	22.153755832952253
44-45	22.37907761300182	27.29377022756121	28.201049625086267	22.126102534350707
46-47	22.45958885243605	27.25158094377783	27.842535295525	22.44629490826112
48-49	22.432358927867718	27.431271686160642	27.761101947807255	22.37526743816439
50-51	22.41164868895609	27.543113132084322	27.5940960088142	22.451142170145385
52-53	22.563404262789206	27.5026061200696	27.438701585572865	22.495288031568332
54-55	22.593141007453564	27.36390775923755	27.49007410738344	22.55287712592545
56-57	22.58087304484361	27.4952761841693	27.45825474709375	22.465596023893337
58-59	22.477205709088587	27.61001288496962	27.50535202531915	22.40742938062264
60-61	22.672653860181924	27.4850451513813	27.456379935081713	22.38592105335506
62-63	22.58682197949314	27.59646962612624	27.320517279207618	22.496191115173
64-65	22.56965467393538	27.53169681601544	27.361911159032143	22.536737351017038
66-67	22.70083539187471	27.51850729827324	27.230000328242365	22.550656981609695
68-69	22.58827517873574	27.44294727832531	27.344677302698965	22.62410024023999
70-71	22.581696745163693	27.251957270079053	27.38264535057542	22.783700634181834
72-73	22.695243039193556	27.283931129615556	27.28760422505164	22.733221606139246
74-75	22.482586826527154	27.462763825651454	27.29725146709291	22.757397880728483
76-77	22.640896844377682	27.348995129597046	27.244386335464426	22.76572169056084
78-79	22.62694485115079	27.346769122128006	27.175567225835866	22.85071880088534
80-81	22.64250750363717	27.34189858830239	27.231939995704728	22.783653912355714
82-83	22.636855208872156	27.309794383060083	27.37325504538304	22.680095362684725
84-85	22.829397566745456	27.300444416087643	27.219243844756612	22.65091417241029
86-87	22.634406516341237	27.28075307874095	27.33004151177668	22.754798893141132
88-89	22.66060397366519	27.26522451419066	27.285267237854903	22.788904274289248
90-91	22.737242871983714	27.296153907584632	27.313119913384163	22.65348330704749
92-93	22.81178928855446	27.177915313514216	27.325826723894288	22.684468674037035
94-95	22.608767854105853	27.245564179986676	27.384296820763314	22.761371145144157
96-97	22.73474052708063	27.33020660386257	27.412219422365926	22.52283344669087
98-99	23.83994294309214	29.282332519540898	27.55185504967254	19.32586948769442
100	22.751049763065527	30.265859673439166	21.33783459315608	25.645255970339225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1508.0
1	1412.5
2	2104.5
3	3134.5
4	4376.0
5	7166.5
6	9554.5
7	11520.5
8	13331.5
9	13984.5
10	13200.0
11	11961.5
12	12736.5
13	16423.5
14	25327.0
15	37231.0
16	46765.0
17	52268.0
18	53063.5
19	49774.0
20	44453.0
21	38708.0
22	35239.0
23	36096.5
24	41161.0
25	50242.5
26	62949.0
27	77101.0
28	90212.0
29	105686.0
30	120789.5
31	134842.5
32	151542.5
33	167849.5
34	184802.0
35	200389.5
36	216494.5
37	233320.0
38	244792.0
39	252540.5
40	262933.0
41	273725.5
42	280515.5
43	288353.0
44	298284.0
45	307971.0
46	314698.0
47	318194.0
48	319787.0
49	319627.5
50	318091.0
51	313285.0
52	304194.0
53	290895.5
54	277216.0
55	261508.5
56	243160.0
57	222538.5
58	200473.0
59	177429.0
60	150688.5
61	124405.0
62	99018.0
63	76690.0
64	58635.0
65	44160.5
66	33711.5
67	26071.5
68	20021.0
69	14369.5
70	10077.0
71	7181.5
72	4841.5
73	3242.5
74	2157.5
75	1376.5
76	871.0
77	575.5
78	377.0
79	249.5
80	180.0
81	128.5
82	88.5
83	49.5
84	24.5
85	15.0
86	11.0
87	10.5
88	7.0
89	4.0
90	3.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.5
96	1.5
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017935741696206487
4	0.01167122901680683
5	0.018117698496023075
6	1.2996914272613396E-5
7	1.2996914272613396E-5
8	1.2996914272613396E-5
9	1.2996914272613396E-5
10-11	1.2996914272613396E-5
12-13	1.2996914272613396E-5
14-15	6.498457136306698E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00415383274277186
26-27	0.04370116622284838
28-29	0.09043912431136801
30-31	0.0873730328034649
32-33	0.03631020137623308
34-35	0.003127604082828136
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.1420339810783528E-4
56-57	2.880476983944725E-4
58-59	1.4532191674667243E-5
60-61	0.0
62-63	7.3948154061809424E-6
64-65	1.4918340361487797E-5
66-67	1.5056986931288194E-5
68-69	1.520265480920402E-5
70-71	2.3027378785797018E-5
72-73	0.011932261635446627
74-75	0.02478043045069965
76-77	0.005201751387857265
78-79	0.0
80-81	0.0
82-83	0.006289525149801355
84-85	0.04419842644397279
86-87	0.04161159006098328
88-89	0.01161956698138846
90-91	1.4442748223903037E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	390.0
22-23	940.0
24-25	3221.0
26-27	4198.0
28-29	7970.0
30-31	19700.0
32-33	24513.0
34-35	33285.0
36-37	48335.0
38-39	57992.0
40-41	58088.0
42-43	67885.0
44-45	80730.0
46-47	78789.0
48-49	68415.0
50-51	61622.0
52-53	57837.0
54-55	60841.0
56-57	62594.0
58-59	61344.0
60-61	59531.0
62-63	57931.0
64-65	60434.0
66-67	63823.0
68-69	63818.0
70-71	62127.0
72-73	58765.0
74-75	59776.0
76-77	59965.0
78-79	63638.0
80-81	64920.0
82-83	61418.0
84-85	62249.0
86-87	64723.0
88-89	68636.0
90-91	72250.0
92-93	73672.0
94-95	82539.0
96-97	238613.0
98-99	455394.0
100-101	4981124.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.49212594259548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38131120041497	73.79143565716771
2	7.9978496432613415	13.355149393702362
3	2.0185094199429567	5.055889281185781
4	0.7652695429989165	2.5557592425639206
5	0.3350509417522051	1.3987057712980164
6	0.18535189089409643	0.9285254050936869
7	0.09836717332258157	0.574901909656627
8	0.060763089865894265	0.40585916413955925
9	0.041369094520074	0.31085942848210396
>10	0.11395016838756324	1.3995077556422764
>50	0.00170517405663471	0.09210787630280108
>100	4.004631214450218E-4	0.06035003700824907
>500	7.66482784599088E-5	0.03410948069518806
>1k	2.554918283863469E-5	0.036839597061641964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5993828545226792E-5	0.0	0.0	0.0
3	0.0	3.899074281784019E-5	0.0	0.0	0.0
4	0.0	1.2996914272613396E-4	0.0	0.0	0.0
5	0.0	1.4296605699874737E-4	0.0	0.0	0.0
6	0.0	1.4296605699874737E-4	0.0	0.0	0.0
7	0.0	1.4296605699874737E-4	0.0	0.0	0.0
8	0.0	1.8195679981658754E-4	0.0	0.0	0.0
9	0.0	3.249228568153349E-4	0.0	2.5993828545226792E-5	0.0
10-11	0.0	3.249228568153349E-4	0.0	2.5993828545226792E-5	0.0
12-13	0.0	3.249228568153349E-4	0.0	3.899074281784019E-5	0.0
14-15	0.0	4.4189508526885544E-4	0.0	3.899074281784019E-5	0.0
16-17	0.0	4.6788891381408226E-4	0.0	3.899074281784019E-5	0.0
18-19	0.0	4.8738428522300233E-4	0.0	3.899074281784019E-5	0.0
20-21	0.0	5.198765709045358E-4	0.0	3.899074281784019E-5	0.0
22-23	0.0	6.433472564943631E-4	0.0	3.899074281784019E-5	0.0
24-25	0.0	7.798148563568038E-4	0.0	9.747685704460048E-5	0.0
26-27	0.0	8.253040563109507E-4	0.0	2.2094754263442772E-4	0.0
28-29	0.0	8.447994277198707E-4	0.0	5.913595994039095E-4	0.0
30-31	0.0	0.0010202577704001516	0.0	0.0012866945129887263	0.0
32-33	0.0	0.0010527500560816852	0.0	0.003028281025518921	0.0
34-35	0.0	0.0011892176559441256	0.0	0.005159774966227518	0.0
36-37	0.0	0.0013711744557607132	0.0	0.0082465421059732	0.0
38-39	0.0	0.0014361590271237804	0.0	0.013672753814789293	0.0
40-41	0.0	0.0015466327984409941	0.0	0.01873505192397221	0.0
42-43	0.0	0.0016246142840766744	0.0	0.023413941062113034	0.0
44-45	0.0	0.001806571083893262	0.0	0.02892463271370111	0.0
46-47	0.0	0.001962534055164623	0.0	0.03446781665097073	0.0
48-49	0.0	0.0019755309694372363	0.0	0.04008898207387602	0.0
50-51	0.0	0.002189980054935357	0.0	0.04556068298264626	0.0
52-53	0.0	0.002235469254889504	0.0	0.051038882348552805	0.0
54-55	0.0	0.002254964626298424	0.0	0.05614666965768987	0.0
56-57	0.0	0.002365438397615638	0.0	0.0615078967951429	0.0
58-59	0.0	0.002365438397615638	0.0	0.06682363473264177	0.0
60-61	0.0	0.002384933769024558	0.0	0.07270473844099934	0.0
62-63	0.0	0.0024499183403876254	0.0	0.07824142392113265	0.0
64-65	0.0	0.0027033581687035866	0.0	0.08395356774394623	0.0
66-67	0.0	0.0027358504543851195	0.0	0.08957473316685152	0.0
68-69	0.0	0.00277484119720296	0.0	0.09521539396116574	0.0
70-71	0.0	0.002807333482884493	0.0	0.10073908252702643	0.0
72-73	0.0	0.00283332731142972	0.0	0.10456017532317477	0.0
74-75	0.0	0.0028788165113838673	0.0	0.10796536686259947	0.0
76-77	0.0	0.0030412779397915347	0.0	0.11100014634525471	0.0
78-79	0.0	0.0031192594254272153	0.0	0.11309264954314546	0.0
80-81	0.0	0.0031192594254272153	0.0	0.11372299988536722	0.0
82-83	0.0	0.0031192594254272153	0.0	0.11421688262772653	0.0
84-85	0.0	0.0031322563396998285	0.0	0.11444432862749726	0.0
86-87	0.0	0.003210237825335509	0.0	0.11454830394167817	0.0
88	0.0	0.00340519153942471	0.0	0.11456779931308708	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12385	0.0	52.613	1
GTATCAA	22890	0.0	37.894638	1
ATCAACG	28290	0.0	30.153765	3
TCAACGC	28270	0.0	30.144632	4
TATCAAC	28660	0.0	29.926563	2
CAACGCA	28840	0.0	29.588161	5
AACGCAG	29175	0.0	29.316013	6
ACGCAGA	33680	0.0	25.382202	7
CGCAGAG	34050	0.0	25.106388	8
GTGGTAT	5535	0.0	23.804556	1
TGGTATC	5575	0.0	22.194527	2
GCAGAGT	38640	0.0	21.96015	9
GAGTACT	22460	0.0	20.440704	12-13
GTACATG	27620	0.0	19.907211	1
TACATGG	27110	0.0	19.8767	2
CAGAGTA	37690	0.0	19.612654	10-11
ACATGGG	27875	0.0	18.967607	3
AGAGTAC	35205	0.0	18.9525	10-11
GTACTTT	24520	0.0	18.586044	14-15
GGACCGA	1545	0.0	17.487503	6
>>END_MODULE