FastQCFastQC Report
Fri 27 May 2016
SRR2138639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7662721
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140300.045.9357221
CTTATAC78450.037.4886781
GTATCAA240650.034.5881041
TATACAC105600.030.6152443
ATCAACG282950.028.8639583
TCAACGC283700.028.802194
TTATACA107650.028.656412
CAACGCA288900.028.2509755
AACGCAG297100.027.5658956
TATCAAC301200.027.4662952
ACGCAGA336800.024.253797
CGCAGAG342900.023.7976518
ACACATC133450.022.126176
TACACAT144000.022.0367495
ACATCTC140150.020.9174888
GCAGAGT388800.020.8576459
GAGTACT229500.020.37724512-13
GTACATG259000.019.949141
TGGTATC74450.019.836682
GTGGTAT78200.019.6430131
Produced by FastQC (version 0.11.5)