##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7662721
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82497170391562	33.0	33.0	33.0	33.0	33.0
2	32.82503408384567	33.0	33.0	33.0	33.0	33.0
3	32.819875602935305	33.0	33.0	33.0	33.0	33.0
4	36.772339616697515	37.0	37.0	37.0	37.0	37.0
5	36.78523621047928	37.0	37.0	37.0	37.0	37.0
6	36.792943263887594	37.0	37.0	37.0	37.0	37.0
7	36.7776531339194	37.0	37.0	37.0	37.0	37.0
8	36.7775754852617	37.0	37.0	37.0	37.0	37.0
9	36.775808227912776	37.0	37.0	37.0	37.0	37.0
10-11	36.75399985201079	37.0	37.0	37.0	37.0	37.0
12-13	36.73770975088353	37.0	37.0	37.0	37.0	37.0
14-15	39.06693660124125	40.0	40.0	40.0	37.0	40.0
16-17	39.01840057859343	40.0	40.0	40.0	37.0	40.0
18-19	39.05700305413704	40.0	40.0	40.0	37.0	40.0
20-21	39.07681728811695	40.0	40.0	40.0	37.0	40.0
22-23	39.10727173903714	40.0	40.0	40.0	37.0	40.0
24-25	39.106476730557475	40.0	40.0	40.0	37.0	40.0
26-27	39.021540171244254	40.0	40.0	40.0	37.0	40.0
28-29	38.9524400303911	40.0	40.0	40.0	37.0	40.0
30-31	38.8814228162897	40.0	40.0	40.0	37.0	40.0
32-33	38.84554053186132	40.0	40.0	40.0	37.0	40.0
34-35	38.8174281172277	40.0	37.0	40.0	37.0	40.0
36-37	38.78673165307552	40.0	37.0	40.0	37.0	40.0
38-39	38.73414565351347	40.0	37.0	40.0	37.0	40.0
40-41	38.698902489204535	40.0	37.0	40.0	37.0	40.0
42-43	38.632862394476675	40.0	37.0	40.0	37.0	40.0
44-45	38.54743635745627	40.0	37.0	40.0	37.0	40.0
46-47	38.467695487504066	40.0	37.0	40.0	37.0	40.0
48-49	38.42949677854777	40.0	37.0	40.0	37.0	40.0
50-51	38.37470982067438	40.0	37.0	40.0	37.0	40.0
52-53	38.313490632026244	40.0	37.0	40.0	37.0	40.0
54-55	38.23447746637976	40.0	37.0	40.0	37.0	40.0
56-57	38.13036566603874	40.0	37.0	40.0	37.0	40.0
58-59	38.019461623668725	40.0	37.0	40.0	37.0	40.0
60-61	37.847478698991495	40.0	37.0	40.0	33.0	40.0
62-63	37.66443584182663	38.5	37.0	40.0	33.0	40.0
64-65	37.44984884654387	37.0	37.0	40.0	33.0	40.0
66-67	37.22242348249253	37.0	37.0	40.0	33.0	40.0
68-69	36.98682713769721	37.0	37.0	40.0	33.0	40.0
70-71	36.729271603313386	37.0	37.0	40.0	33.0	40.0
72-73	36.48248632895679	37.0	37.0	38.5	33.0	40.0
74-75	36.239253783134686	37.0	37.0	37.0	33.0	40.0
76-77	34.62849746836954	35.0	33.0	37.0	30.0	37.0
78-79	35.84985359474403	37.0	37.0	37.0	33.0	37.0
80-81	35.95193610754061	37.0	37.0	37.0	33.0	37.0
82-83	35.87907198734743	37.0	37.0	37.0	33.0	37.0
84-85	35.75449002562074	37.0	37.0	37.0	33.0	37.0
86-87	35.644440899034706	37.0	37.0	37.0	33.0	37.0
88-89	35.57003137643568	37.0	37.0	37.0	33.0	37.0
90-91	35.50361137935569	37.0	35.0	37.0	33.0	37.0
92-93	35.43452090340794	37.0	33.0	37.0	33.0	37.0
94-95	35.392241422726755	37.0	33.0	37.0	33.0	37.0
96-97	35.35572720514874	37.0	33.0	37.0	33.0	37.0
98-99	35.31457607488838	37.0	33.0	37.0	33.0	37.0
100	35.25642370521068	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	1.0
19	6.0
20	36.0
21	137.0
22	470.0
23	1326.0
24	3178.0
25	6657.0
26	12134.0
27	19729.0
28	29762.0
29	42568.0
30	58637.0
31	79612.0
32	107482.0
33	147112.0
34	212893.0
35	330321.0
36	654568.0
37	2113372.0
38	3674466.0
39	168250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37324065433153	20.33644445621862	14.78007616354556	23.510238725904284
2	14.407153803459632	20.60891946868482	42.33904640401236	22.64488032384319
3	17.967157233147628	26.155741480991363	30.8046517030187	25.07244958284231
4	12.30584139246814	18.36852344573269	38.6153523679356	30.710282793863563
5	13.209989230330866	37.68054140984119	35.422970238461176	13.686499121366772
6	29.415269066954153	37.83066093623923	18.80983269520057	13.94423730160605
7	26.193241278130834	33.05151003148881	22.619615147152036	18.135633543228312
8	24.365822532231043	36.526124336250795	21.056071335495577	18.051981796022588
9	25.267068969364797	17.102032032746592	21.316461867788217	36.314437130100394
10-11	24.081811147763307	26.862618383208787	28.536873520515755	20.518696948512154
12-13	25.069234544752444	24.4703415405572	28.67459874893005	21.785825165760308
14-15	22.211874867948346	25.843104557767404	26.581249663141854	25.363770911142396
16-17	21.49988887759322	29.134507180934815	27.210732323413577	22.15487161805839
18-19	21.481103122507005	27.837370041268628	29.426942465998696	21.25458437022567
20-21	22.25926385843806	26.798423496245988	29.383580089184868	21.558732556131087
22-23	22.042621012682382	27.06592366867744	29.275750554096398	21.615704764543782
24-25	21.923929444888163	27.25107474114332	29.33972982879539	21.485265985173125
26-27	21.728901442724172	27.304833461772343	29.398846635982416	21.56741845952107
28-29	21.391202837013942	27.2061658292854	29.660934978557265	21.741696355143393
30-31	21.840288415007947	27.038422488335666	29.25029998489518	21.870989111761208
32-33	21.826702119189367	27.206184332393242	29.428807496395788	21.538306052021603
34-35	21.80764719795116	27.550373939446267	29.33696146114753	21.305017401455043
36-37	21.759152182087266	27.376817388163836	29.039684847948642	21.82434558180025
38-39	21.70713778686657	27.260806976806425	29.267959021542268	21.76409621478474
40-41	21.821352453147842	27.182816446866077	29.297369011091885	21.698462088894196
42-43	21.931081958855103	27.198472207699066	29.10213249516539	21.76831333828045
44-45	21.796607357958766	27.51360650892538	28.799281755387224	21.89050437772863
46-47	22.174697723600538	27.345665134353304	28.23962668871529	22.240010453330875
48-49	22.0503647771687	27.526935248137445	28.200798044469526	22.221901930224327
50-51	21.934246650784566	27.68362336093097	28.20344566610087	22.1786843221836
52-53	21.997170728450083	27.777849778979007	28.26001912758868	21.96496036498223
54-55	22.027829631865988	27.80042068361833	27.970683188861617	22.201066495654064
56-57	22.242272994007426	28.030561148403795	27.627181321234207	22.099984536354572
58-59	21.851650204383194	28.293956596438953	28.063348919539038	21.791044279638815
60-61	22.25846804504142	28.369432975510378	27.574136461787273	21.797962517660928
62-63	21.992685361597218	28.554659355664892	27.521854056053535	21.930801226684352
64-65	21.989905714130174	28.44112295858361	27.57356963173871	21.995401695547503
66-67	22.15860541487579	28.207347928285394	27.496191037910815	22.137855618928
68-69	22.15679800439541	27.981121431817037	27.656262656458114	22.205817907329447
70-71	22.15411341644012	27.702468881137182	27.752137449469373	22.391280252953326
72-73	22.22919868167449	27.728696516451173	27.703382354335176	22.33872244753916
74-75	22.110906799316673	27.83953138360909	27.714097639289097	22.335464177785138
76-77	22.202875152838946	27.81274706489868	27.633249840730933	22.351127941531438
78-79	22.16857556006516	27.78598702662569	27.601113126734067	22.44432428657508
80-81	22.186164825196563	27.80316729789984	27.652807952302844	22.357859924600753
82-83	22.251115797854094	27.70820851175427	27.745674358734874	22.295001331656763
84-85	22.405216599551203	27.724960168011076	27.58141745910941	22.288405773328307
86-87	22.215348787040444	27.735746356082164	27.736845809562165	22.31205904731523
88-89	22.289134463577824	27.649622468414293	27.704151487795293	22.35709158021259
90-91	22.37509858345679	27.668620203063394	27.653761864626052	22.302519348853767
92-93	22.37874381216144	27.57044226358974	27.73221350905925	22.31860041518957
94-95	22.220298345750784	27.621284848616224	27.785151342699223	22.373265462933773
96-97	22.324092189429575	27.77333047282679	27.780472844578075	22.12210449316556
98-99	23.426230714371933	29.667630537306987	27.98565783570085	18.92048091262023
100	22.402486378715878	30.63702586147037	21.782260319037235	25.178227440776524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1410.0
1	1350.0
2	2061.5
3	3085.5
4	4403.5
5	7148.5
6	9396.0
7	11161.5
8	12822.5
9	13382.5
10	12694.0
11	11755.5
12	12770.0
13	16659.0
14	25735.5
15	38052.5
16	48401.0
17	54742.5
18	55712.5
19	52051.5
20	46367.5
21	40261.0
22	36491.5
23	37429.0
24	42706.5
25	51857.5
26	64363.0
27	78390.5
28	93108.5
29	109777.0
30	126598.0
31	144022.5
32	163158.5
33	181292.5
34	198997.0
35	215060.5
36	231487.5
37	247914.0
38	259463.0
39	266509.5
40	274639.5
41	283569.0
42	289718.5
43	296069.5
44	303486.0
45	311799.0
46	318401.5
47	323331.5
48	332849.5
49	329097.0
50	314433.5
51	306533.0
52	293628.5
53	273331.5
54	252670.5
55	232169.5
56	212105.0
57	191723.0
58	170009.5
59	148703.5
60	125805.5
61	103622.5
62	82689.0
63	63961.0
64	48833.0
65	36599.5
66	27506.0
67	20942.5
68	15867.5
69	11404.0
70	7994.0
71	5744.5
72	3992.0
73	2660.0
74	1776.5
75	1162.0
76	760.5
77	451.5
78	281.5
79	193.0
80	135.5
81	95.5
82	68.0
83	44.5
84	27.5
85	19.0
86	13.5
87	9.5
88	7.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0019705793803532715
4	0.010635908576078915
5	0.018139770454907597
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.003968820476345928
26-27	0.04235516668384121
28-29	0.08819135007320202
30-31	0.08480180073153411
32-33	0.03569817868482402
34-35	0.0030114403831130784
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2067788601874283E-4
56-57	3.3663218550352943E-4
58-59	1.4462693229716815E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.848485140036753E-5
72-73	0.01206511682614555
74-75	0.02522938117455392
76-77	0.005175482079537188
78-79	0.0
80-81	0.0
82-83	0.005921749222729631
84-85	0.043322647684458856
86-87	0.041262447734024724
88-89	0.010901247826695246
90-91	1.8743281704963877E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	680.0
22-23	1265.0
24-25	3097.0
26-27	4027.0
28-29	6725.0
30-31	15765.0
32-33	20335.0
34-35	28281.0
36-37	40906.0
38-39	49351.0
40-41	52047.0
42-43	61308.0
44-45	74313.0
46-47	72387.0
48-49	63410.0
50-51	56622.0
52-53	54133.0
54-55	60181.0
56-57	66848.0
58-59	65597.0
60-61	66528.0
62-63	66904.0
64-65	72557.0
66-67	80073.0
68-69	67648.0
70-71	61213.0
72-73	58250.0
74-75	59297.0
76-77	59472.0
78-79	63334.0
80-81	64390.0
82-83	61896.0
84-85	62518.0
86-87	64610.0
88-89	69256.0
90-91	72699.0
92-93	74943.0
94-95	82780.0
96-97	236494.0
98-99	451915.0
100-101	4968511.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.12103765765832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39760720114043	73.47700837012881
2	7.970704368090385	13.250664358762048
3	2.019049493878453	5.034764670400405
4	0.7594477596935549	2.525043433300489
5	0.32298509080600724	1.342342794787416
6	0.17519641123530252	0.8737504497465705
7	0.09961916522533779	0.579631386888385
8	0.05834393113329608	0.3879686477461221
9	0.04404655688435116	0.32950759621269077
>10	0.14971126511532548	1.8890914764926028
>50	0.0023477868999595944	0.13197889149823505
>100	8.86034948103079E-4	0.13642107412649399
>500	2.7467475044577256E-5	0.017146268956833632
>1k	2.7467474382578375E-5	0.024680580952887818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.9150583715627906E-5	0.0	0.0	0.0
3	0.0	3.9150583715627906E-5	0.0	0.0	0.0
4	0.0	7.830116743125581E-5	0.0	0.0	0.0
5	0.0	7.830116743125581E-5	0.0	0.0	0.0
6	0.0	7.830116743125581E-5	0.0	0.0	0.0
7	0.0	7.830116743125581E-5	0.0	1.305019457187597E-5	0.0
8	0.0	9.135136200313179E-5	0.0	1.305019457187597E-5	0.0
9	0.0	2.2185330772189147E-4	0.0	1.305019457187597E-5	0.0
10-11	0.0	2.2185330772189147E-4	0.0	1.305019457187597E-5	0.0
12-13	0.0	2.3490350229376746E-4	0.0	1.305019457187597E-5	0.0
14-15	0.0	3.197297670109613E-4	0.0	3.9150583715627906E-5	0.0
16-17	0.0	3.3930505886877523E-4	0.0	3.9150583715627906E-5	0.0
18-19	0.0	3.523552534406512E-4	0.0	6.525097285937985E-5	1.305019457187597E-5
20-21	0.0	3.6540544801252716E-4	0.0	1.305019457187597E-4	1.305019457187597E-5
22-23	0.0	4.045560317281551E-4	0.0	1.9575291857813954E-4	1.305019457187597E-5
24-25	0.0	4.37181518157845E-4	0.0	3.327799615828372E-4	1.305019457187597E-5
26-27	0.0	4.5675681001565893E-4	0.0	4.5023171272972094E-4	1.305019457187597E-5
28-29	0.0	4.5675681001565893E-4	0.0	7.830116743125582E-4	1.305019457187597E-5
30-31	0.0	5.481081720187908E-4	0.0	0.0013115445544735348	1.9575291857813953E-5
32-33	0.0	5.611583665906666E-4	0.0	0.0027013902763783257	3.9150583715627906E-5
34-35	0.0	6.720850204516124E-4	0.0	0.004658919462159721	5.220077828750388E-5
36-37	0.0	7.895367715984962E-4	0.0	0.007621313629975566	5.220077828750388E-5
38-39	0.0	8.22162258028186E-4	0.0	0.01191482764412276	5.220077828750388E-5
40-41	0.0	9.918147874625736E-4	0.0	0.016404094576848095	5.220077828750388E-5
42-43	0.0	0.0010440155657500776	0.0	0.020834635633999985	5.220077828750388E-5
44-45	0.0	0.0011679924141828992	0.0	0.025924211517031615	5.220077828750388E-5
46-47	0.0	0.001252818678900093	0.0	0.031594521058511724	6.525097285937985E-5
48-49	0.0	0.001259343776186031	0.0	0.037356181961994965	6.525097285937985E-5
50-51	0.0	0.0014224712083344805	0.0	0.04301996640618913	6.525097285937985E-5
52-53	0.0	0.0014420465001922945	0.0	0.048709851239527056	7.830116743125581E-5
54-55	0.0	0.0014681468893360467	0.0	0.05471946583987594	9.135136200313179E-5
56-57	0.0	0.0015007723757657365	0.0	0.06083348199679983	9.135136200313179E-5
58-59	0.0	0.0015007723757657365	0.0	0.06676479542971747	9.135136200313179E-5
60-61	0.0	0.0015464480567673024	0.0	0.07223935205261943	9.135136200313179E-5
62-63	0.0	0.0015790735431969922	0.0	0.07749858046508545	9.135136200313179E-5
64-65	0.0	0.0018531276292063877	0.0	0.083332017438714	9.135136200313179E-5
66-67	0.0	0.0018792280183501397	0.0	0.08904147756390973	9.135136200313179E-5
68-69	0.0	0.0019966797694970233	0.0	0.09450298399223983	9.787645928906977E-5
70-71	0.0	0.002061930742356403	0.0	0.09931198069197614	1.0440155657500775E-4
72-73	0.0	0.002107606423357969	0.0	0.10330534023097018	1.1745175114688373E-4
74-75	0.0	0.002198957785361101	0.0	0.10617638303678288	1.1745175114688373E-4
76-77	0.0	0.0022903091473642327	0.0	0.10917140269102843	1.1745175114688373E-4
78-79	0.0	0.0023620852175095506	0.0	0.11112893187680982	1.1745175114688373E-4
80-81	0.0	0.0023751354120814267	0.0	0.1118662678701208	1.1745175114688373E-4
82-83	0.0	0.0023947107039392406	0.0	0.11234912506928021	1.1745175114688373E-4
84-85	0.0	0.002414285995797054	0.0	0.11255792818243024	1.1745175114688373E-4
86-87	0.0	0.0024730118713704964	0.0	0.11262317915528962	1.1745175114688373E-4
88	0.0	0.002570888330659566	0.0	0.11263622934986149	1.1745175114688373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14030	0.0	45.935722	1
CTTATAC	7845	0.0	37.488678	1
GTATCAA	24065	0.0	34.588104	1
TATACAC	10560	0.0	30.615244	3
ATCAACG	28295	0.0	28.863958	3
TCAACGC	28370	0.0	28.80219	4
TTATACA	10765	0.0	28.65641	2
CAACGCA	28890	0.0	28.250975	5
AACGCAG	29710	0.0	27.565895	6
TATCAAC	30120	0.0	27.466295	2
ACGCAGA	33680	0.0	24.25379	7
CGCAGAG	34290	0.0	23.797651	8
ACACATC	13345	0.0	22.12617	6
TACACAT	14400	0.0	22.036749	5
ACATCTC	14015	0.0	20.917488	8
GCAGAGT	38880	0.0	20.857645	9
GAGTACT	22950	0.0	20.377245	12-13
GTACATG	25900	0.0	19.94914	1
TGGTATC	7445	0.0	19.83668	2
GTGGTAT	7820	0.0	19.643013	1
>>END_MODULE