FastQCFastQC Report
Fri 27 May 2016
SRR2138638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5804796
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC81050.055.455751
GGTATCA86300.049.3154261
TTATACA107100.042.5930022
TATACAC109900.042.460723
GTATCAA159450.036.963191
ATCAACG191000.030.2872733
TCAACGC193300.029.969724
TATCAAC197550.029.4951062
CAACGCA196500.029.478875
TACACAT159600.029.3662455
CTCGTAT273700.029.0109799
AACGCAG200950.028.9893156
TCTCGTA272400.028.9034448
AATCTCG274500.028.7586086
ATCTCGT276150.028.571617
AAATCTC298950.026.577865
ACACATC169700.026.553966
GAAATCT304350.026.0950054
ACATCTC174500.025.919538
ATACACA190150.025.5316854
Produced by FastQC (version 0.11.5)