##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2138638_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5804796 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.804638957165764 33.0 33.0 33.0 33.0 33.0 2 32.819054106294175 33.0 33.0 33.0 33.0 33.0 3 32.81576820270686 33.0 33.0 33.0 33.0 33.0 4 36.7570769067509 37.0 37.0 37.0 37.0 37.0 5 36.74332052323631 37.0 37.0 37.0 37.0 37.0 6 36.77019381904205 37.0 37.0 37.0 37.0 37.0 7 36.760091482973735 37.0 37.0 37.0 37.0 37.0 8 36.69106287283825 37.0 37.0 37.0 37.0 37.0 9 36.69067371187549 37.0 37.0 37.0 37.0 37.0 10-11 36.72842473706225 37.0 37.0 37.0 37.0 37.0 12-13 36.711792025077195 37.0 37.0 37.0 37.0 37.0 14-15 39.01940946761953 40.0 40.0 40.0 37.0 40.0 16-17 38.97668626080916 40.0 40.0 40.0 37.0 40.0 18-19 39.023953641092646 40.0 40.0 40.0 37.0 40.0 20-21 38.99620787187049 40.0 40.0 40.0 37.0 40.0 22-23 39.060161632672475 40.0 40.0 40.0 37.0 40.0 24-25 39.05443103104548 40.0 40.0 40.0 37.0 40.0 26-27 38.93502021552055 40.0 40.0 40.0 37.0 40.0 28-29 38.88029556605384 40.0 40.0 40.0 37.0 40.0 30-31 38.73717532917324 40.0 38.5 40.0 37.0 40.0 32-33 38.740262741071874 40.0 37.0 40.0 37.0 40.0 34-35 38.72882790767827 40.0 37.0 40.0 37.0 40.0 36-37 38.69915725267026 40.0 37.0 40.0 37.0 40.0 38-39 38.63614338570309 40.0 37.0 40.0 37.0 40.0 40-41 38.64592180694531 40.0 37.0 40.0 37.0 40.0 42-43 38.60987497992612 40.0 37.0 40.0 37.0 40.0 44-45 38.53671681568157 40.0 37.0 40.0 37.0 40.0 46-47 38.46639768105023 40.0 37.0 40.0 37.0 40.0 48-49 38.44187904558861 40.0 37.0 40.0 37.0 40.0 50-51 38.353137654142415 40.0 37.0 40.0 37.0 40.0 52-53 38.25946507327095 40.0 37.0 40.0 37.0 40.0 54-55 38.20030727292472 40.0 37.0 40.0 37.0 40.0 56-57 38.08788795083727 40.0 37.0 40.0 37.0 40.0 58-59 37.97504373219395 40.0 37.0 40.0 37.0 40.0 60-61 37.80510596184507 40.0 37.0 40.0 33.0 40.0 62-63 37.61861503335832 38.5 37.0 40.0 33.0 40.0 64-65 37.40921774256572 37.0 37.0 40.0 33.0 40.0 66-67 37.194197401621366 37.0 37.0 40.0 33.0 40.0 68-69 36.97540882915127 37.0 37.0 40.0 33.0 40.0 70-71 36.72602479728347 37.0 37.0 40.0 33.0 40.0 72-73 36.48321200324029 37.0 37.0 38.5 33.0 40.0 74-75 36.24707168747023 37.0 37.0 37.0 33.0 40.0 76-77 34.67109540576459 37.0 33.0 37.0 30.0 37.0 78-79 35.848109930947466 37.0 37.0 37.0 33.0 37.0 80-81 35.94714901495692 37.0 37.0 37.0 33.0 37.0 82-83 35.87482103389381 37.0 37.0 37.0 33.0 37.0 84-85 35.75375629006452 37.0 37.0 37.0 33.0 37.0 86-87 35.64796550638334 37.0 37.0 37.0 33.0 37.0 88-89 35.573502784097 37.0 37.0 37.0 33.0 37.0 90-91 35.509658075679525 37.0 35.0 37.0 33.0 37.0 92-93 35.44113363689574 37.0 33.0 37.0 33.0 37.0 94-95 35.40595046511871 37.0 33.0 37.0 33.0 37.0 96-97 35.376291348288035 37.0 33.0 37.0 33.0 37.0 98-99 35.33447983858441 37.0 33.0 37.0 33.0 37.0 100 35.27603392152027 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 5.0 19 11.0 20 39.0 21 129.0 22 455.0 23 1291.0 24 2863.0 25 6090.0 26 10555.0 27 16918.0 28 25147.0 29 35094.0 30 47869.0 31 64173.0 32 86869.0 33 117761.0 34 169226.0 35 259730.0 36 509681.0 37 1617755.0 38 2716714.0 39 116420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64025746985768 20.1637576927768 14.521578363821916 23.674406473543602 2 14.045144738936562 20.149648669824057 41.64456425342079 24.16064233781859 3 17.374341375933184 27.534857439167986 30.622330295285817 24.468470889613013 4 13.530404380954481 18.420866735400494 37.36173404970889 30.686994833936133 5 12.902358204161748 38.65908970300701 34.875725114870875 13.562826977960368 6 28.747918100825594 38.84758396332963 18.400750000516812 14.00374793532796 7 25.48976742679674 34.20087458715172 21.842714197019156 18.466643789032382 8 23.969128286334264 35.7345546682433 22.556382687694796 17.739934357727645 9 24.556401292999787 16.739950895776527 20.48657696153319 38.2170708496905 10-11 23.45733596839579 26.29828851866629 28.913203840410585 21.33117167252734 12-13 25.391012879694653 23.77538332096425 28.80886770181071 22.024736097530386 14-15 21.898900495383472 26.1586281412818 26.446166239089198 25.496305124245538 16-17 22.226508907462037 28.75727243472467 26.21584806770126 22.80037059011204 18-19 22.16749735908032 27.456908046380956 28.123555074114577 22.252039520424148 20-21 22.37384852183811 26.286731596699443 28.939626680880536 22.39979320058191 22-23 21.762940545189505 27.040592792496952 29.57023612152253 21.62623054079101 24-25 21.892933321431638 27.342336691427022 28.934616602798442 21.8301133843429 26-27 22.662198693302297 27.581171123805206 27.996038763284375 21.760591419608122 28-29 21.307142532270518 27.20876356879871 29.90148674774223 21.582607151188547 30-31 23.158444657469712 27.640486506393092 28.147271199888152 21.053797636249044 32-33 21.105046708651525 29.145199694665024 28.395468862211747 21.3542847344717 34-35 21.540110304977002 28.99019674303381 28.68199344725143 20.787699504737752 36-37 21.356091862907547 28.765651108825736 28.350738489636353 21.527518538630368 38-39 21.603272032728093 27.59939238014771 28.881949290235028 21.915386296889174 40-41 21.81980706963083 27.02100838683072 29.213641180701526 21.945543362836922 42-43 22.10844781723693 26.788940218394046 29.287368770450588 21.815243193918434 44-45 21.78053567540843 27.333413306984504 28.859141279583273 22.026909738023797 46-47 22.4948375953412 26.76824137743059 28.008358393468313 22.728562633759896 48-49 22.229455747342946 26.893526757960025 28.302715603072475 22.574301891624554 50-51 21.894543695967652 27.371936032642523 28.339518705765666 22.394001565624155 52-53 21.92546789355077 27.65726463363008 28.556539122790497 21.86072835002865 54-55 21.94626339353994 27.712323054225845 27.958919035997614 22.382494516236594 56-57 22.46136557745884 28.107103000856 27.303625200125982 22.127906221559183 58-59 21.729174044396647 28.61383644577755 28.1290231967955 21.527966313030305 60-61 22.349334253948577 29.04897764631113 27.017696997277383 21.583991102462907 62-63 21.8909815426962 29.397630975548754 26.953310272244064 21.75807720951098 64-65 21.935498197576486 28.996008503082145 27.17980907855544 21.88868422078593 66-67 22.14871170986788 28.571163566654405 27.20471741113982 22.075407312337887 68-69 22.230595771853643 28.030302325458784 27.46922281157539 22.26987909111218 70-71 22.257249503616748 27.75480824112649 27.577738510180776 22.410203745075986 72-73 22.379292252452714 27.70148511513435 27.548285206162976 22.370937426249956 74-75 22.227662568874308 27.895834362695798 27.495703241840943 22.38079982658895 76-77 22.3338470329481 27.906661033578768 27.34775956715084 22.411732366322294 78-79 22.266360524526032 27.88274000818896 27.365233396271037 22.485666071013966 80-81 22.256166043724875 27.890831928118757 27.439970509858064 22.413031518298297 82-83 22.360498711552072 27.859546677317383 27.53957375779381 22.240380853336735 84-85 22.530064997017092 27.813850179280074 27.38542765387257 22.270657169830262 86-87 22.283253332370037 27.85592893576525 27.453813172331465 22.40700455953325 88-89 22.366861063806752 27.78816737090893 27.451086988529617 22.393884576754704 90-91 22.389698215884334 27.844672545888166 27.470169173160368 22.29546006506713 92-93 22.420327592106712 27.812834399728654 27.513763078439567 22.253074929725067 94-95 22.268072117739003 27.884368798817572 27.515105328031353 22.332453755412075 96-97 22.36070059028055 28.005900780795834 27.526075260204735 22.10732336871888 98-99 23.454656223071243 29.88117630465154 27.78407938532687 18.880088086950344 100 22.38131721284026 30.9031542857389 21.630433963300323 25.085094538120522 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1497.0 1 1320.0 2 1695.0 3 2400.5 4 3309.5 5 5315.0 6 6941.0 7 7998.0 8 8715.0 9 8636.0 10 7944.5 11 7400.0 12 8459.0 13 11614.5 14 18507.5 15 27473.0 16 34767.5 17 39326.5 18 39999.5 19 37166.5 20 32824.5 21 28252.0 22 25505.0 23 26287.5 24 30150.5 25 36928.5 26 45876.5 27 55843.0 28 66691.0 29 79091.0 30 91054.5 31 102414.0 32 115537.0 33 128554.0 34 141641.0 35 155648.0 36 177709.5 37 208717.0 38 233573.5 39 245401.5 40 248347.0 41 244199.0 42 234545.0 43 228000.5 44 230478.5 45 244378.5 46 266164.0 47 281359.5 48 274724.0 49 261556.5 50 253346.0 51 237537.0 52 218775.0 53 201847.0 54 186962.5 55 173176.0 56 158834.0 57 143512.5 58 127156.5 59 110566.5 60 92407.0 61 74213.5 62 58345.0 63 44911.0 64 33246.5 65 24249.5 66 18087.5 67 13859.5 68 10423.5 69 7503.5 70 5478.5 71 3867.0 72 2515.5 73 1553.0 74 1000.5 75 689.5 76 457.0 77 297.5 78 201.0 79 134.5 80 85.5 81 54.5 82 35.5 83 20.0 84 13.0 85 10.5 86 8.5 87 8.0 88 6.0 89 3.5 90 3.0 91 3.0 92 2.5 93 2.5 94 2.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0013953978744472675 4 0.013161530568860644 5 0.015297695216162635 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004126938422546283 26-27 0.04148851197409821 28-29 0.08519555613770602 30-31 0.0810457360592398 32-33 0.033044816350772416 34-35 0.002881619511827616 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.0449776427033343E-4 56-57 2.590155146456006E-4 58-59 2.9098680025676674E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.0444126014646843E-5 72-73 0.012862459852217605 74-75 0.024911005565405465 76-77 0.004060715735969905 78-79 0.0 80-81 0.0 82-83 0.006276391814660132 84-85 0.04239964666961109 86-87 0.03875609530994054 88-89 0.010464003826835686 90-91 1.8311970512233883E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 74.0 20-21 312.0 22-23 750.0 24-25 1530.0 26-27 2641.0 28-29 5500.0 30-31 11921.0 32-33 16124.0 34-35 25448.0 36-37 57890.0 38-39 73247.0 40-41 56772.0 42-43 46068.0 44-45 53887.0 46-47 52267.0 48-49 45822.0 50-51 40456.0 52-53 39784.0 54-55 47781.0 56-57 57465.0 58-59 55955.0 60-61 59424.0 62-63 60747.0 64-65 68079.0 66-67 73876.0 68-69 52893.0 70-71 41905.0 72-73 39569.0 74-75 40076.0 76-77 40125.0 78-79 42490.0 80-81 42804.0 82-83 40825.0 84-85 41037.0 86-87 42864.0 88-89 45693.0 90-91 47836.0 92-93 49199.0 94-95 59827.0 96-97 174042.0 98-99 335099.0 100-101 3714692.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.69659702170947 #Duplication Level Percentage of deduplicated Percentage of total 1 89.74705215856086 76.91016962718531 2 7.329909282149188 12.562965639160534 3 1.6910714491315384 4.347572055334312 4 0.6103022686041165 2.0920331029600825 5 0.2585769904979909 1.107958407689636 6 0.13025854982302848 0.6697628671689801 7 0.0768435129179075 0.46096592921809115 8 0.044198524653702644 0.3030130524961957 9 0.02881594976330808 0.22224859511976333 >10 0.08071182194905918 1.009412340248877 >50 0.0012914135275013633 0.07647253439054182 >100 8.451487888103265E-4 0.15925874649956315 >500 9.219723715990857E-5 0.04751127990280744 >1k 3.073239579044877E-5 0.030655822625265547 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.72271342524354E-5 2 0.0 1.72271342524354E-5 0.0 0.0 1.72271342524354E-5 3 0.0 1.72271342524354E-5 0.0 0.0 1.72271342524354E-5 4 0.0 3.44542685048708E-5 0.0 0.0 1.72271342524354E-5 5 3.44542685048708E-5 3.44542685048708E-5 0.0 0.0 1.72271342524354E-5 6 3.44542685048708E-5 3.44542685048708E-5 0.0 0.0 1.72271342524354E-5 7 3.44542685048708E-5 3.44542685048708E-5 0.0 0.0 1.72271342524354E-5 8 3.44542685048708E-5 3.44542685048708E-5 0.0 0.0 5.16814027573062E-5 9 3.44542685048708E-5 5.16814027573062E-5 0.0 0.0 6.89085370097416E-5 10-11 5.16814027573062E-5 5.16814027573062E-5 0.0 0.0 6.89085370097416E-5 12-13 1.033628055146124E-4 5.16814027573062E-5 0.0 0.0 6.89085370097416E-5 14-15 1.033628055146124E-4 6.89085370097416E-5 0.0 0.0 8.6135671262177E-5 16-17 1.2058993976704781E-4 6.89085370097416E-5 0.0 8.6135671262177E-6 8.6135671262177E-5 18-19 1.378170740194832E-4 7.75221041359593E-5 0.0 1.72271342524354E-5 9.47492383883947E-5 20-21 1.378170740194832E-4 8.6135671262177E-5 0.0 2.5840701378653103E-5 1.205899397670478E-4 22-23 1.72271342524354E-4 8.6135671262177E-5 0.0 3.44542685048708E-5 1.205899397670478E-4 24-25 2.239527452816602E-4 9.47492383883947E-5 0.0 3.44542685048708E-5 1.205899397670478E-4 26-27 2.411798795340956E-4 1.033628055146124E-4 0.0 8.6135671262177E-5 1.205899397670478E-4 28-29 2.411798795340956E-4 1.033628055146124E-4 0.0 3.4454268504870795E-4 1.205899397670478E-4 30-31 2.58407013786531E-4 1.205899397670478E-4 0.0 0.0011972858305442603 1.205899397670478E-4 32-33 2.756341480389664E-4 1.378170740194832E-4 0.0 0.0030836570311859367 1.205899397670478E-4 34-35 2.756341480389664E-4 1.6365777539813632E-4 0.0 0.005624659333420158 1.205899397670478E-4 36-37 2.756341480389664E-4 1.894984767767894E-4 0.0 0.009294038929188897 1.205899397670478E-4 38-39 2.756341480389664E-4 2.067256110292248E-4 0.0 0.014591382711812782 1.205899397670478E-4 40-41 2.756341480389664E-4 2.239527452816602E-4 0.0 0.020164360642475634 1.205899397670478E-4 42-43 2.756341480389664E-4 2.411798795340956E-4 0.0 0.025892382781410407 1.205899397670478E-4 44-45 2.756341480389664E-4 2.7563414803896636E-4 0.0 0.032421466663083424 1.205899397670478E-4 46-47 2.842477151651841E-4 2.928612822914018E-4 0.0 0.03988081579438795 1.205899397670478E-4 48-49 2.928612822914018E-4 2.928612822914018E-4 0.0 0.04664246598846884 1.205899397670478E-4 50-51 2.928612822914018E-4 3.014748494176195E-4 0.0 0.053714204599093575 1.205899397670478E-4 52-53 2.928612822914018E-4 3.100884165438372E-4 0.0 0.06069119397132991 1.205899397670478E-4 54-55 2.928612822914018E-4 3.100884165438372E-4 0.0 0.06671207739255608 1.205899397670478E-4 56-57 2.928612822914018E-4 3.100884165438372E-4 0.0 0.0711480644625582 1.205899397670478E-4 58-59 2.928612822914018E-4 3.100884165438372E-4 0.0 0.07597166205324012 1.205899397670478E-4 60-61 3.100884165438372E-4 3.100884165438372E-4 0.0 0.08035596772048492 1.205899397670478E-4 62-63 3.100884165438372E-4 3.100884165438372E-4 0.0 0.08542935875782715 1.205899397670478E-4 64-65 3.100884165438372E-4 3.617698193011434E-4 0.0 0.09031325131839257 1.205899397670478E-4 66-67 3.100884165438372E-4 3.617698193011434E-4 0.0 0.09505932680493853 1.205899397670478E-4 68-69 3.100884165438372E-4 3.617698193011434E-4 0.0 0.09966758521746501 1.292035068932655E-4 70-71 3.100884165438372E-4 3.617698193011434E-4 0.0 0.10382793813942816 1.378170740194832E-4 72-73 3.100884165438372E-4 3.617698193011434E-4 0.0 0.10746286346669202 1.464306411457009E-4 74-75 3.100884165438372E-4 3.617698193011434E-4 0.0 0.11019336424570303 1.550442082719186E-4 76-77 3.100884165438372E-4 3.617698193011434E-4 0.0 0.11290663789046161 1.550442082719186E-4 78-79 3.273155507962726E-4 3.617698193011434E-4 0.0 0.1145604427786954 1.6365777539813632E-4 80-81 3.273155507962726E-4 3.617698193011434E-4 0.0 0.11506864323914226 1.808849096505717E-4 82-83 3.273155507962726E-4 3.617698193011434E-4 0.0 0.11549932159545315 1.894984767767894E-4 84-85 3.273155507962726E-4 3.617698193011434E-4 0.0 0.11567159293797749 1.9811204390300711E-4 86-87 3.273155507962726E-4 3.703833864273611E-4 0.0 0.11573188790786101 2.067256110292248E-4 88 3.273155507962726E-4 4.134512220584496E-4 0.0 0.11573188790786101 2.067256110292248E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 8105 0.0 55.45575 1 GGTATCA 8630 0.0 49.315426 1 TTATACA 10710 0.0 42.593002 2 TATACAC 10990 0.0 42.46072 3 GTATCAA 15945 0.0 36.96319 1 ATCAACG 19100 0.0 30.287273 3 TCAACGC 19330 0.0 29.96972 4 TATCAAC 19755 0.0 29.495106 2 CAACGCA 19650 0.0 29.47887 5 TACACAT 15960 0.0 29.366245 5 CTCGTAT 27370 0.0 29.010979 9 AACGCAG 20095 0.0 28.989315 6 TCTCGTA 27240 0.0 28.903444 8 AATCTCG 27450 0.0 28.758608 6 ATCTCGT 27615 0.0 28.57161 7 AAATCTC 29895 0.0 26.57786 5 ACACATC 16970 0.0 26.55396 6 GAAATCT 30435 0.0 26.095005 4 ACATCTC 17450 0.0 25.91953 8 ATACACA 19015 0.0 25.531685 4 >>END_MODULE