FastQCFastQC Report
Fri 27 May 2016
SRR2138637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7975979
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123400.051.7802121
GTATCAA231200.039.0926781
ATCAACG270000.033.0023463
TCAACGC281950.031.6627144
CAACGCA287500.031.002465
TATCAAC289350.031.001042
AACGCAG292050.030.5460766
ACGCAGA295800.030.1875677
CGCAGAG342750.026.1392658
GTGGTAT58600.025.7543581
TGGTATC58600.023.7228812
GCAGAGT382500.023.2783819
GAGTACT229850.021.29215412-13
GTACATG269650.019.99121
CAGAGTA377950.019.94892310-11
TACATGG268350.019.5017572
ACATGGG261650.019.3999613
AGAGTAC352700.019.37071810-11
GTACTTT254150.019.17327914-15
AGTACTT247900.018.03596712-13
Produced by FastQC (version 0.11.5)