FastQCFastQC Report
Fri 27 May 2016
SRR2138636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477048
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC118700.057.821631
GGTATCA96250.050.3789861
TTATACA155250.044.7235532
TATACAC162150.043.9356773
GTATCAA175450.037.704761
TACACAT224550.031.4595975
TCAACGC214500.030.1733474
ATCAACG214650.030.1332743
CAACGCA218750.029.6218055
ACACATC231400.029.5596876
TATCAAC221500.029.4861832
AACGCAG225850.028.7038356
ACATCTC247400.027.8519048
ATACACA274100.027.0646044
CACATCT253100.026.9588897
ACGCAGA259750.024.9576937
CGCAGAG265800.024.4212518
CATCTCC282500.024.3467229
GTGGTAT47650.022.6825081
TGGTATC45100.022.4730172
Produced by FastQC (version 0.11.5)