##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7477048
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81174335111932	33.0	33.0	33.0	33.0	33.0
2	32.81349912425331	33.0	33.0	33.0	33.0	33.0
3	32.81198876882962	33.0	33.0	33.0	33.0	33.0
4	36.75736226382391	37.0	37.0	37.0	37.0	37.0
5	36.77094931047654	37.0	37.0	37.0	37.0	37.0
6	36.77626290482554	37.0	37.0	37.0	37.0	37.0
7	36.76012739252175	37.0	37.0	37.0	37.0	37.0
8	36.7597904948584	37.0	37.0	37.0	37.0	37.0
9	36.76097170969078	37.0	37.0	37.0	37.0	37.0
10-11	36.73660273412716	37.0	37.0	37.0	37.0	37.0
12-13	36.71840631489861	37.0	37.0	37.0	37.0	37.0
14-15	39.02866512292017	40.0	40.0	40.0	37.0	40.0
16-17	38.969761863238006	40.0	40.0	40.0	37.0	40.0
18-19	38.99908613666784	40.0	40.0	40.0	37.0	40.0
20-21	39.01773412776683	40.0	40.0	40.0	37.0	40.0
22-23	39.050957967966056	40.0	40.0	40.0	37.0	40.0
24-25	39.05617107027122	40.0	40.0	40.0	37.0	40.0
26-27	38.96131920494504	40.0	40.0	40.0	37.0	40.0
28-29	38.87339877525845	40.0	40.0	40.0	37.0	40.0
30-31	38.79874038277303	40.0	38.5	40.0	37.0	40.0
32-33	38.76439694246611	40.0	37.0	40.0	37.0	40.0
34-35	38.72145132519195	40.0	37.0	40.0	37.0	40.0
36-37	38.67375198410643	40.0	37.0	40.0	37.0	40.0
38-39	38.60811218801474	40.0	37.0	40.0	37.0	40.0
40-41	38.58922211530207	40.0	37.0	40.0	37.0	40.0
42-43	38.53797769543215	40.0	37.0	40.0	37.0	40.0
44-45	38.441259376041096	40.0	37.0	40.0	37.0	40.0
46-47	38.34847022196627	40.0	37.0	40.0	37.0	40.0
48-49	38.30990045619649	40.0	37.0	40.0	37.0	40.0
50-51	38.20316004370711	40.0	37.0	40.0	37.0	40.0
52-53	38.155519302148996	40.0	37.0	40.0	37.0	40.0
54-55	38.09391019246762	40.0	37.0	40.0	37.0	40.0
56-57	37.951594981455344	40.0	37.0	40.0	33.0	40.0
58-59	37.85407208549977	40.0	37.0	40.0	33.0	40.0
60-61	37.671530912463936	40.0	37.0	40.0	33.0	40.0
62-63	37.50639929076846	37.0	37.0	40.0	33.0	40.0
64-65	37.30133821432316	37.0	37.0	40.0	33.0	40.0
66-67	37.082025459961855	37.0	37.0	40.0	33.0	40.0
68-69	36.848683185045644	37.0	37.0	40.0	33.0	40.0
70-71	36.587326876114545	37.0	37.0	40.0	33.0	40.0
72-73	36.34107662173219	37.0	37.0	37.0	33.0	40.0
74-75	36.104724789326305	37.0	37.0	37.0	33.0	40.0
76-77	34.50636006725469	35.0	33.0	37.0	30.0	37.0
78-79	35.736262233894905	37.0	35.0	37.0	33.0	37.0
80-81	35.8536196183604	37.0	37.0	37.0	33.0	37.0
82-83	35.78753227273583	37.0	37.0	37.0	33.0	37.0
84-85	35.666759401034554	37.0	37.0	37.0	33.0	37.0
86-87	35.55949033571804	37.0	37.0	37.0	33.0	37.0
88-89	35.48655240767691	37.0	35.0	37.0	33.0	37.0
90-91	35.42158073214937	37.0	33.0	37.0	33.0	37.0
92-93	35.35314360809467	37.0	33.0	37.0	33.0	37.0
94-95	35.30975228969371	37.0	33.0	37.0	33.0	37.0
96-97	35.27829094609274	37.0	33.0	37.0	33.0	37.0
98-99	35.23754666687691	37.0	33.0	37.0	33.0	37.0
100	35.178152741822686	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	10.0
20	32.0
21	141.0
22	586.0
23	1692.0
24	4114.0
25	7957.0
26	13844.0
27	21944.0
28	32674.0
29	46473.0
30	62663.0
31	84653.0
32	114185.0
33	155671.0
34	223939.0
35	347799.0
36	688637.0
37	2119939.0
38	3402706.0
39	147388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.055667958798715	19.988276121806358	14.37515179787531	24.580904121519616
2	14.400910626760721	20.225990257117516	42.23546511938937	23.1376339967324
3	18.199043322011818	25.53501009440391	30.792940671386525	25.473005912197742
4	12.284621330669232	18.188588764361754	37.177210379667834	32.34957952530118
5	13.393685774036973	37.20503644180908	35.32276476727297	14.078513016880972
6	30.23875197805337	37.02204399383286	18.261150657318236	14.478053370795532
7	26.388395527218762	32.60366925556717	21.665381845883562	19.342553371330503
8	24.54828429615538	36.46425701694038	21.01298533859887	17.974473348305374
9	24.81641150357735	16.96797987655021	20.35133384191194	37.864274777960496
10-11	24.170748937281132	26.464762564049344	28.224929143159173	21.139559355510357
12-13	25.075263660203866	23.82831432939845	28.237454139655117	22.85896787074257
14-15	22.322191859675105	25.520131741831804	25.559625937937003	26.598050460556088
16-17	22.175262215783555	29.06702618466539	26.199477387332543	22.55823421221851
18-19	22.096982659466676	27.456972323836894	28.294348250807005	22.151696765889426
20-21	22.586159578284104	27.00007168763664	28.080201078470946	22.333567655608313
22-23	22.62095226579621	27.28918818368144	27.916884212626965	22.172975337895384
24-25	22.62649408206743	27.380700027311526	28.147723862054775	21.845082028566267
26-27	22.374355105114955	27.918396181328013	28.240539065054026	21.466709648503002
28-29	21.8379274226446	27.05145313916333	28.835118007754435	22.275501430437632
30-31	22.255295646715535	27.637182550364024	28.19946133093018	21.908060471990254
32-33	23.071317578149607	26.94876090576317	28.425734585771767	21.55418693031546
34-35	22.265268074667244	27.077741933330497	28.663365932727952	21.993624059274307
36-37	21.99819631060577	27.603241709687293	28.243000068955826	22.155561910751107
38-39	22.050679996618424	26.711792971232068	28.706854235014028	22.53067279713548
40-41	22.07625066294104	26.612459687177648	28.91949793265377	22.39179171722754
42-43	22.402295007024932	26.515395199531927	29.0050324308754	22.077277362567745
44-45	21.939377355071795	27.0793199099314	28.621262906998723	22.360039827998083
46-47	22.76791776429535	26.517116453955193	27.72229002427555	22.992675757473897
48-49	22.365323351578425	26.608463159649983	28.147954124865176	22.878259363906416
50-51	22.10833007040534	27.16169607391732	28.188677098134452	22.541296757542888
52-53	22.20167141329607	27.449190623875026	28.43227877696203	21.91685918586688
54-55	22.073493698007677	27.534909240386803	27.787862543856455	22.603734517749064
56-57	22.63670026964834	28.018913856546142	27.100984799427952	22.24340107437757
58-59	21.907234627670547	28.42656537882035	27.970023990208443	21.69617600330066
60-61	22.5035159089406	28.833699696531077	26.889176315472383	21.773608079055933
62-63	21.990974062288426	29.224786701702605	26.88717745915706	21.897061776851913
64-65	22.124355572732025	28.794022960314926	27.01896028271808	22.062661184234976
66-67	22.301429605508375	28.372321919871553	27.053466751136156	22.27278172348392
68-69	22.401325855498115	27.88085547698155	27.30271172412495	22.415106943395376
70-71	22.471444316538136	27.52029514228601	27.41304511109743	22.595215430078426
72-73	22.49761945249481	27.53206775791664	27.39650931103308	22.573803478555472
74-75	22.358740762703647	27.6842327553376	27.35046192019593	22.606564561762823
76-77	22.48737669118658	27.684703479992418	27.235587846039866	22.59233198278114
78-79	22.481680229027095	27.69259276919247	27.195848545704166	22.629878456076266
80-81	22.456879047364446	27.693223253535344	27.224255257121378	22.625642441978837
82-83	22.518668079106263	27.62759092083373	27.346164175248628	22.50757682481138
84-85	22.638814221197716	27.616113094370938	27.252069960296126	22.49300272413522
86-87	22.436483658487123	27.618387643643977	27.349163792941123	22.59596490492778
88-89	22.54127789944326	27.651122988466465	27.284645524039377	22.5229535880509
90-91	22.567455863240887	27.666984131033416	27.27812571305473	22.487434292670965
92-93	22.5381872164712	27.65812994964515	27.315881823561373	22.487801010322272
94-95	22.440921636885065	27.746612526710752	27.319884443947036	22.49258139245715
96-97	22.532812899110716	27.80804149496447	27.40074554804684	22.258400057877974
98-99	23.64193242614782	29.6933092089906	27.605675623704183	19.059082741157397
100	22.490624969955174	30.785065496670722	21.44585933212081	25.278450201253293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2051.0
1	1768.5
2	2009.0
3	2724.5
4	3677.5
5	5809.0
6	7678.0
7	9137.5
8	10241.0
9	10402.0
10	9953.0
11	9412.5
12	10360.5
13	13661.5
14	20787.0
15	30224.5
16	38212.5
17	43031.0
18	43830.5
19	41413.0
20	37365.0
21	33118.5
22	30809.0
23	32064.5
24	36846.0
25	44891.0
26	56695.5
27	70229.5
28	83143.5
29	96957.5
30	110838.0
31	124407.0
32	140178.5
33	157149.5
34	174001.0
35	189681.0
36	206825.0
37	222885.5
38	233226.5
39	242398.5
40	254452.5
41	265738.5
42	274019.0
43	283214.0
44	295091.5
45	314970.5
46	348327.0
47	374888.5
48	370287.5
49	355712.5
50	344227.5
51	325060.0
52	302438.5
53	280571.0
54	262032.5
55	244595.0
56	225462.0
57	205120.5
58	184846.5
59	162657.0
60	136968.5
61	111702.0
62	88690.0
63	68843.0
64	51882.5
65	38293.5
66	28607.5
67	21838.5
68	16410.0
69	11800.5
70	8419.0
71	5910.0
72	3949.0
73	2567.0
74	1713.0
75	1133.5
76	730.0
77	462.5
78	314.0
79	211.0
80	139.5
81	94.5
82	68.5
83	48.0
84	32.0
85	19.0
86	10.5
87	6.5
88	4.5
89	3.5
90	3.0
91	3.5
92	3.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017787768648803645
4	0.011434994131373772
5	0.017306295211693173
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.687279089914814E-6
22-23	0.0
24-25	0.004220633587333872
26-27	0.0424394855956987
28-29	0.08841618146666425
30-31	0.08502726449780555
32-33	0.035733852492377406
34-35	0.0031420874181312605
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	8.750683191359575E-5
56-57	2.2847754430566522E-4
58-59	1.4921601532508164E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	8.059063912325766E-6
72-73	0.012595451814062183
74-75	0.025762704413033976
76-77	0.004824251603836099
78-79	0.0
80-81	0.0
82-83	0.0063751495479006095
84-85	0.04274106389595751
86-87	0.04029877997742193
88-89	0.0114700011277228
90-91	1.6829834868317613E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	383.0
22-23	875.0
24-25	2003.0
26-27	3553.0
28-29	7125.0
30-31	18217.0
32-33	22571.0
34-35	30171.0
36-37	45207.0
38-39	51300.0
40-41	51849.0
42-43	60274.0
44-45	70416.0
46-47	68628.0
48-49	61819.0
50-51	55092.0
52-53	55105.0
54-55	68135.0
56-57	81728.0
58-59	79299.0
60-61	79323.0
62-63	82656.0
64-65	89484.0
66-67	104387.0
68-69	68624.0
70-71	56547.0
72-73	52883.0
74-75	53727.0
76-77	53904.0
78-79	56680.0
80-81	56731.0
82-83	54774.0
84-85	54942.0
86-87	57370.0
88-89	60709.0
90-91	63588.0
92-93	65800.0
94-95	78918.0
96-97	229721.0
98-99	437915.0
100-101	4784518.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.27138366933899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2127141458926	77.82775673932704
2	7.04858319896567	12.161820509664482
3	1.5985658178547593	4.137314549785158
4	0.5458494106227351	1.8836473571806616
5	0.23807914800495455	1.0269708760602387
6	0.12291920613765321	0.6362645995819229
7	0.07200272957968151	0.43482425761658566
8	0.0420154749362352	0.2899786526618754
9	0.02787718928546119	0.21645033232219274
>10	0.08924814894978153	1.160373905823848
>50	0.0015774822565652442	0.0886677988692474
>100	4.5012699699372057E-4	0.06677331428048404
>500	9.433656454263197E-5	0.04647109150774631
>1k	2.358395242318109E-5	0.022686015318527576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6748524283915258E-5
2	0.0	0.0	0.0	1.3374262141957629E-5	2.6748524283915258E-5
3	0.0	0.0	0.0	1.3374262141957629E-5	2.6748524283915258E-5
4	0.0	4.012278642587288E-5	0.0	2.6748524283915258E-5	5.3497048567830516E-5
5	1.3374262141957629E-5	4.012278642587288E-5	0.0	2.6748524283915258E-5	5.3497048567830516E-5
6	1.3374262141957629E-5	4.012278642587288E-5	0.0	2.6748524283915258E-5	8.024557285174576E-5
7	1.3374262141957629E-5	4.012278642587288E-5	0.0	4.012278642587288E-5	8.024557285174576E-5
8	1.3374262141957629E-5	5.3497048567830516E-5	0.0	6.687131070978814E-5	8.024557285174576E-5
9	1.3374262141957629E-5	1.2036835927761865E-4	0.0	1.3374262141957629E-4	1.0699409713566103E-4
10-11	2.6748524283915258E-5	1.2036835927761865E-4	0.0	1.6049114570349153E-4	1.0699409713566103E-4
12-13	2.6748524283915258E-5	1.2036835927761865E-4	0.0	2.0061393212936443E-4	1.0699409713566103E-4
14-15	2.6748524283915258E-5	1.2705549034859747E-4	0.0	2.0061393212936443E-4	1.1368122820663984E-4
16-17	2.6748524283915258E-5	1.404297524905551E-4	0.0	2.0730106320034325E-4	1.2036835927761865E-4
18-19	2.6748524283915258E-5	1.872396699874068E-4	0.0	2.2736245641327967E-4	1.2705549034859747E-4
20-21	2.6748524283915258E-5	2.2736245641327967E-4	0.0	2.407367185552373E-4	1.3374262141957629E-4
22-23	2.6748524283915258E-5	2.407367185552373E-4	0.0	2.4742384962621615E-4	1.538040146325127E-4
24-25	3.343565535489407E-5	2.6079811176817373E-4	0.0	2.87546636052089E-4	1.939268010583856E-4
26-27	4.012278642587288E-5	2.6748524283915257E-4	0.0	3.6110507783285596E-4	2.0061393212936443E-4
28-29	4.012278642587288E-5	2.6748524283915257E-4	0.0	7.355844178076696E-4	2.0730106320034325E-4
30-31	4.012278642587288E-5	2.808595049811102E-4	0.0	0.0020195135834356016	2.1398819427132206E-4
32-33	4.012278642587288E-5	2.808595049811102E-4	0.0	0.004701053142898107	2.1398819427132206E-4
34-35	5.349704856783051E-5	3.209822914069831E-4	0.0	0.007609955158773891	2.6748524283915257E-4
36-37	6.687131070978814E-5	3.6110507783285596E-4	0.0	0.012244136990962207	2.808595049811102E-4
38-39	6.687131070978814E-5	4.146021264006865E-4	0.0	0.018630347163746976	2.808595049811102E-4
40-41	6.687131070978814E-5	4.5472491282655934E-4	0.0	0.025049992991886635	2.808595049811102E-4
42-43	8.024557285174576E-5	4.68099174968517E-4	0.0	0.03130914767432281	2.87546636052089E-4
44-45	8.024557285174576E-5	5.617190099622203E-4	0.0	0.038725176032038316	3.343565535489407E-4
46-47	8.024557285174576E-5	6.419645828139661E-4	0.0	0.04644881241901884	3.677922089038348E-4
48-49	8.024557285174576E-5	6.419645828139661E-4	0.0	0.05414570028171546	3.878536021167712E-4
50-51	8.024557285174576E-5	7.690200731625636E-4	0.0	0.06118724929945615	3.878536021167712E-4
52-53	8.024557285174576E-5	7.757072042335425E-4	0.0	0.06788775463257693	4.146021264006865E-4
54-55	8.024557285174576E-5	7.757072042335425E-4	0.0	0.07422715488786483	4.5472491282655934E-4
56-57	8.024557285174576E-5	7.890814663755E-4	0.0	0.08006502031282933	4.68099174968517E-4
58-59	8.024557285174576E-5	7.890814663755E-4	0.0	0.08633754925740747	4.68099174968517E-4
60-61	8.693270392272458E-5	8.024557285174576E-4	0.0	0.0926702623816244	4.68099174968517E-4
62-63	9.36198349937034E-5	8.29204252801373E-4	0.0	0.09890266853977667	4.814734371104746E-4
64-65	9.36198349937034E-5	9.763211363629069E-4	0.0	0.10480740527545096	4.814734371104746E-4
66-67	9.36198349937034E-5	9.896953985048644E-4	0.0	0.11115349266180985	4.814734371104746E-4
68-69	9.36198349937034E-5	0.0010164439227887798	0.0	0.1171986591499747	4.814734371104746E-4
70-71	9.36198349937034E-5	0.0010365053160017162	0.0	0.12239455999212523	4.814734371104746E-4
72-73	9.36198349937034E-5	0.001043192447072695	0.0	0.1265405812561321	5.349704856783051E-4
74-75	9.36198349937034E-5	0.0010565667092146526	0.0	0.12958991302449843	5.416576167492839E-4
76-77	9.36198349937034E-5	0.001076628102427589	0.0	0.13252556356465814	5.483447478202627E-4
78-79	9.36198349937034E-5	0.001083315233498568	0.0	0.1345250157548808	5.483447478202627E-4
80-81	9.36198349937034E-5	0.0010966894956405257	0.0	0.1351870417309077	5.617190099622203E-4
82-83	9.36198349937034E-5	0.0011100637577824831	0.0	0.1355347725465986	5.884675342461357E-4
84-85	9.36198349937034E-5	0.0011234380199244406	0.0	0.135722012216586	5.884675342461357E-4
86-87	9.36198349937034E-5	0.00118362219956325	0.0	0.1357888835272958	5.884675342461357E-4
88	9.36198349937034E-5	0.001230432117060102	0.0	0.1357888835272958	6.018417963880933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	11870	0.0	57.82163	1
GGTATCA	9625	0.0	50.378986	1
TTATACA	15525	0.0	44.723553	2
TATACAC	16215	0.0	43.935677	3
GTATCAA	17545	0.0	37.70476	1
TACACAT	22455	0.0	31.459597	5
TCAACGC	21450	0.0	30.173347	4
ATCAACG	21465	0.0	30.133274	3
CAACGCA	21875	0.0	29.621805	5
ACACATC	23140	0.0	29.559687	6
TATCAAC	22150	0.0	29.486183	2
AACGCAG	22585	0.0	28.703835	6
ACATCTC	24740	0.0	27.851904	8
ATACACA	27410	0.0	27.064604	4
CACATCT	25310	0.0	26.958889	7
ACGCAGA	25975	0.0	24.957693	7
CGCAGAG	26580	0.0	24.421251	8
CATCTCC	28250	0.0	24.346722	9
GTGGTAT	4765	0.0	22.682508	1
TGGTATC	4510	0.0	22.473017	2
>>END_MODULE