FastQCFastQC Report
Fri 27 May 2016
SRR2138635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8671522
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133300.049.571241
GTATCAA241450.036.9582141
ATCAACG293450.029.9610923
TCAACGC294750.029.9003774
CAACGCA297800.029.5776865
TATCAAC303700.029.1593742
AACGCAG304500.028.9470526
CGCAGAG344700.025.534268
ACGCAGA344850.025.5231537
GCAGAGT401300.021.901179
GTGGTAT65250.020.6049331
GAGTACT251600.020.34762412-13
TGGTATC62700.019.8871362
GTACATG272950.019.6717531
TACATGG262000.019.6359522
CAGAGTA394000.019.38542210-11
AGAGTAC368100.019.02172710-11
ACATGGG268200.018.596923
GTACTTT278500.018.35229914-15
AGTACTT267300.017.91531612-13
Produced by FastQC (version 0.11.5)