##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8671522
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82914983090627	33.0	33.0	33.0	33.0	33.0
2	32.824133641130125	33.0	33.0	33.0	33.0	33.0
3	32.82260853400361	33.0	33.0	33.0	33.0	33.0
4	36.775657145308514	37.0	37.0	37.0	37.0	37.0
5	36.789586533944096	37.0	37.0	37.0	37.0	37.0
6	36.79598333487478	37.0	37.0	37.0	37.0	37.0
7	36.78274482841651	37.0	37.0	37.0	37.0	37.0
8	36.78198106399315	37.0	37.0	37.0	37.0	37.0
9	36.78128695285557	37.0	37.0	37.0	37.0	37.0
10-11	36.760293233413925	37.0	37.0	37.0	37.0	37.0
12-13	36.74526968852757	37.0	37.0	37.0	37.0	37.0
14-15	39.08917206229772	40.0	40.0	40.0	37.0	40.0
16-17	39.04327481381008	40.0	40.0	40.0	37.0	40.0
18-19	39.07526354658387	40.0	40.0	40.0	37.0	40.0
20-21	39.09014596445369	40.0	40.0	40.0	37.0	40.0
22-23	39.12334151510438	40.0	40.0	40.0	37.0	40.0
24-25	39.1248564761635	40.0	40.0	40.0	37.0	40.0
26-27	39.04657958306413	40.0	40.0	40.0	37.0	40.0
28-29	38.97853460347296	40.0	40.0	40.0	37.0	40.0
30-31	38.90586973447246	40.0	40.0	40.0	37.0	40.0
32-33	38.877626250004994	40.0	40.0	40.0	37.0	40.0
34-35	38.85883118383143	40.0	40.0	40.0	37.0	40.0
36-37	38.827352029529365	40.0	38.5	40.0	37.0	40.0
38-39	38.783767249790756	40.0	37.0	40.0	37.0	40.0
40-41	38.743186458445905	40.0	37.0	40.0	37.0	40.0
42-43	38.679926652271504	40.0	37.0	40.0	37.0	40.0
44-45	38.60046020416952	40.0	37.0	40.0	37.0	40.0
46-47	38.52532637688607	40.0	37.0	40.0	37.0	40.0
48-49	38.48525953361594	40.0	37.0	40.0	37.0	40.0
50-51	38.440563777836616	40.0	37.0	40.0	37.0	40.0
52-53	38.369204089459245	40.0	37.0	40.0	37.0	40.0
54-55	38.30636362560675	40.0	37.0	40.0	37.0	40.0
56-57	38.20925589216199	40.0	37.0	40.0	37.0	40.0
58-59	38.09360556713003	40.0	37.0	40.0	37.0	40.0
60-61	37.93205963136862	40.0	37.0	40.0	35.0	40.0
62-63	37.74820475051356	40.0	37.0	40.0	33.0	40.0
64-65	37.524825064199284	37.0	37.0	40.0	33.0	40.0
66-67	37.29133348999475	37.0	37.0	40.0	33.0	40.0
68-69	37.04800188350825	37.0	37.0	40.0	33.0	40.0
70-71	36.78322660109602	37.0	37.0	40.0	33.0	40.0
72-73	36.53233282205001	37.0	37.0	38.5	33.0	40.0
74-75	36.287146928867955	37.0	37.0	37.0	33.0	40.0
76-77	34.67358878714957	37.0	33.0	37.0	30.0	37.0
78-79	35.8762211161632	37.0	37.0	37.0	33.0	37.0
80-81	35.97374482134405	37.0	37.0	37.0	33.0	37.0
82-83	35.895206568483246	37.0	37.0	37.0	33.0	37.0
84-85	35.76666141588049	37.0	37.0	37.0	33.0	37.0
86-87	35.655659328142605	37.0	37.0	37.0	33.0	37.0
88-89	35.58168815679059	37.0	37.0	37.0	33.0	37.0
90-91	35.51950375037829	37.0	35.0	37.0	33.0	37.0
92-93	35.45290446078908	37.0	33.0	37.0	33.0	37.0
94-95	35.40825142302323	37.0	33.0	37.0	33.0	37.0
96-97	35.37440770114789	37.0	33.0	37.0	33.0	37.0
98-99	35.332929804328614	37.0	33.0	37.0	33.0	37.0
100	35.272565327493226	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	21.0
21	96.0
22	408.0
23	1283.0
24	3155.0
25	6698.0
26	12591.0
27	20971.0
28	32149.0
29	45950.0
30	63442.0
31	87126.0
32	117756.0
33	162599.0
34	234429.0
35	361286.0
36	721141.0
37	2348508.0
38	4249328.0
39	202579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77510014966231	20.228974798195747	14.58033549358463	23.415589558557308
2	14.466687624156405	20.988057229169225	42.007896652975106	22.537358493699262
3	18.00684784589233	26.12651562503604	30.68992796961023	25.176708559461396
4	12.315574153358988	18.149547007239903	39.30157666899869	30.23330217040241
5	13.223972859729633	37.75644553162596	35.591754526944364	13.427827081700054
6	29.570068553132888	38.110172585619914	18.895656379583652	13.42410248166354
7	26.15840679410143	33.11809622347726	23.139548051656906	17.583948930764404
8	24.284502766642348	36.57428303820252	21.280370389419527	17.860843805735602
9	25.512130396486338	17.001582882451316	21.543219287225472	35.94306743383687
10-11	23.96929858449301	26.98941431504181	28.94908759961631	20.09219950084887
12-13	24.898570285585393	24.623607020774436	29.10670698869241	21.37111570494776
14-15	22.01407088628732	26.04897963702335	27.074422460094087	24.86252701659524
16-17	21.311016681961945	29.124154906139893	27.750612868190842	21.81421554370732
18-19	21.257715773540102	28.186493674351514	29.725168199999953	20.83062235210843
20-21	22.22082553994231	27.351830045242124	29.590456749317838	20.83688766549772
22-23	21.86939367838015	27.24044837482952	29.482605082048106	21.407552864742225
24-25	21.603127327438422	27.373637522992126	29.60121291966853	21.422022229900925
26-27	21.574817955967752	27.5643961789742	29.437410582506274	21.423375282551778
28-29	21.463684964428268	27.736996063274404	29.456123613380143	21.343195358917185
30-31	21.813808898498205	27.51357073066384	29.334765359881704	21.337855010956254
32-33	21.558579185671405	27.683626591275146	29.270989903185935	21.486804319867513
34-35	21.680484705181442	27.73806166393758	29.239152359848468	21.342301271032515
36-37	21.69257864425031	27.68228000110895	29.186103689739152	21.43903766490159
38-39	21.548492480563013	27.880314294420444	29.162292021710496	21.408901203306055
40-41	21.886212638336016	27.71846388185235	28.97502442093412	21.42029905887751
42-43	21.738754831367306	27.825982720098068	28.789360666983388	21.645901781551242
44-45	21.911453659064566	27.964552600603415	28.5346514113567	21.589342328975317
46-47	21.893732647149438	27.943210565238818	28.22812393847193	21.93493284913981
48-49	21.869037362679446	28.080177614403773	28.198078650688274	21.852706372228504
50-51	21.900863008132017	28.23185960695828	28.00593612584448	21.86134125906522
52-53	22.054961230484736	28.1320169877058	27.843132629504176	21.969889152305296
54-55	22.065660346314715	27.992133407783648	27.946641817469853	21.995564428431784
56-57	22.03729821838384	28.09768000568743	27.897567106852893	21.967454669075835
58-59	21.980632044801684	28.157067149178904	27.929402264213323	21.932898541806093
60-61	22.086675652293042	28.083945634271668	27.916412121600086	21.912966591835204
62-63	22.011707300436896	28.158225521118318	27.82577302202641	22.004294156418382
64-65	21.984722420273677	28.14738337045321	27.82645466713831	22.041439542134803
66-67	22.082063868388236	28.19427418852532	27.643804591410092	22.079857351676356
68-69	22.051659953751635	28.14597437507338	27.74210899374513	22.060256677429855
70-71	22.030027137106227	27.8937903518366	27.793476961157527	22.282705549899646
72-73	22.07360006242681	27.96482325682411	27.76218168602589	22.199394994723185
74-75	21.994839630737975	28.11660982924396	27.660159061231198	22.228391478786865
76-77	22.10848694153599	28.110228097875677	27.571100808697885	22.21018415189045
78-79	22.10901034189088	28.141892337745812	27.513056177434287	22.236041142929025
80-81	22.066161119100624	28.103134143355618	27.53897500509964	22.291729732444118
82-83	22.160330056298562	28.049137459715578	27.67288434583492	22.11764813815094
84-85	22.313712950999655	28.039482037669305	27.529643112027653	22.11716189930339
86-87	22.069234437089193	28.066294452048247	27.64718334881015	22.21728776205241
88-89	22.163151263973493	28.080139106779633	27.564703563523622	22.19200606572325
90-91	22.234505499292755	28.087752749384393	27.601796068108786	22.07594568321406
92-93	22.24940459219234	28.05956798165675	27.610275935992895	22.08075149015801
94-95	22.091969809812134	28.16898024366904	27.63728510616477	22.10176484035405
96-97	22.160221417673394	28.2619400465221	27.752377691903657	21.825460843900853
98-99	23.276876479613655	30.149875569368312	27.89971728880292	18.673530662215114
100	22.151298500848686	31.13525619309854	21.89831174103751	24.815133565015262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2036.0
1	1849.5
2	2568.5
3	3731.0
4	5275.5
5	8756.5
6	11526.5
7	13191.0
8	14037.0
9	13592.0
10	12700.5
11	12083.5
12	13615.0
13	18287.5
14	28461.5
15	41847.5
16	52828.0
17	59620.0
18	61158.0
19	57852.5
20	52265.0
21	46641.0
22	43535.0
23	45488.0
24	51924.5
25	63300.5
26	79597.0
27	97854.5
28	116227.5
29	135942.0
30	155301.5
31	173579.0
32	192956.5
33	212424.0
34	232958.5
35	252349.5
36	272226.0
37	291181.0
38	302984.0
39	311896.0
40	323918.5
41	334790.0
42	341075.5
43	347460.0
44	353126.5
45	357849.5
46	360467.0
47	360599.0
48	357930.0
49	351273.5
50	342501.5
51	328854.0
52	311827.0
53	292879.5
54	273199.5
55	252624.0
56	230997.0
57	208434.0
58	185705.0
59	162549.0
60	135154.0
61	108927.5
62	86387.0
63	66616.5
64	50036.5
65	36794.5
66	27241.0
67	20730.0
68	15757.5
69	11238.0
70	7778.0
71	5378.0
72	3639.0
73	2432.0
74	1673.0
75	1137.5
76	734.0
77	444.5
78	271.5
79	192.5
80	145.0
81	96.0
82	66.5
83	48.0
84	38.0
85	32.5
86	25.5
87	18.0
88	12.5
89	11.0
90	10.5
91	8.5
92	5.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0020988241741184536
4	0.011116848922253786
5	0.018462733531668375
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0038930312664048014
26-27	0.0428535428826196
28-29	0.08919995382128838
30-31	0.08559028165092089
32-33	0.035668972240594836
34-35	0.003062467123514703
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.383677038709749E-4
56-57	3.1075304468222904E-4
58-59	2.5587505109504927E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.377781560893126E-5
72-73	0.012781128561375539
74-75	0.026305191161841296
76-77	0.004949065634480641
78-79	0.0
80-81	0.0
82-83	0.005876929639678277
84-85	0.04583340996370544
86-87	0.043622509156190536
88-89	0.011222518423387817
90-91	1.6467498509878517E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	468.0
22-23	1081.0
24-25	2386.0
26-27	3888.0
28-29	8023.0
30-31	19209.0
32-33	24658.0
34-35	33803.0
36-37	49282.0
38-39	57905.0
40-41	61367.0
42-43	73111.0
44-45	86455.0
46-47	83679.0
48-49	73834.0
50-51	65020.0
52-53	61508.0
54-55	64930.0
56-57	67538.0
58-59	67759.0
60-61	66787.0
62-63	66136.0
64-65	68824.0
66-67	72046.0
68-69	72227.0
70-71	71555.0
72-73	68093.0
74-75	69382.0
76-77	69838.0
78-79	72533.0
80-81	73701.0
82-83	71319.0
84-85	71197.0
86-87	73941.0
88-89	77943.0
90-91	80595.0
92-93	82953.0
94-95	98208.0
96-97	272181.0
98-99	513202.0
100-101	5652865.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.99583697608755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78388693997918	74.5747689351388
2	7.742322376422363	13.00645696292575
3	1.960492956482834	4.940197402965
4	0.7173586309467567	2.4102055447357253
5	0.32568946476291627	1.3678279593527565
6	0.1675045561317451	0.8441811233756379
7	0.09048257783837506	0.5320111900101813
8	0.053745194091001404	0.36114980488927506
9	0.04326745588235683	0.32708575535980544
>10	0.11287009325692912	1.392976547355504
>50	0.0017681745338518462	0.09801267544078611
>100	5.228382363712397E-4	0.07895442354865122
>500	6.655641608080458E-5	0.038201301453920505
>1k	2.218501929129461E-5	0.02797037344818112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1532000956694799E-5	0.0
3	0.0	0.0	0.0	1.1532000956694799E-5	0.0
4	0.0	1.1532000956694799E-5	0.0	2.3064001913389597E-5	0.0
5	0.0	1.1532000956694799E-5	0.0	3.45960028700844E-5	0.0
6	3.45960028700844E-5	1.1532000956694799E-5	0.0	3.45960028700844E-5	0.0
7	3.45960028700844E-5	2.3064001913389597E-5	0.0	3.45960028700844E-5	0.0
8	3.45960028700844E-5	2.3064001913389597E-5	0.0	3.45960028700844E-5	0.0
9	3.45960028700844E-5	4.6128003826779195E-5	0.0	3.45960028700844E-5	0.0
10-11	3.45960028700844E-5	4.6128003826779195E-5	0.0	4.6128003826779195E-5	5.766000478347399E-6
12-13	3.45960028700844E-5	4.6128003826779195E-5	0.0	6.91920057401688E-5	1.1532000956694799E-5
14-15	3.45960028700844E-5	1.326180110019902E-4	0.0	7.49580062185162E-5	1.1532000956694799E-5
16-17	6.91920057401688E-5	1.383840114803376E-4	0.0	9.80220081319058E-5	1.1532000956694799E-5
18-19	6.91920057401688E-5	1.499160124370324E-4	0.0	1.383840114803376E-4	1.1532000956694799E-5
20-21	6.91920057401688E-5	1.614480133937272E-4	0.0	1.72980014350422E-4	1.1532000956694799E-5
22-23	6.91920057401688E-5	1.960440162638116E-4	0.0	1.9604401626381157E-4	1.1532000956694799E-5
24-25	6.91920057401688E-5	2.0757601722050639E-4	0.0	2.364060196122434E-4	1.1532000956694799E-5
26-27	6.91920057401688E-5	2.0757601722050639E-4	0.0	3.45960028700844E-4	1.1532000956694799E-5
28-29	6.91920057401688E-5	2.0757601722050639E-4	0.0	8.187720679253307E-4	1.1532000956694799E-5
30-31	6.91920057401688E-5	2.0757601722050639E-4	0.0	0.0024620822042543396	1.1532000956694799E-5
32-33	7.49580062185162E-5	2.0757601722050639E-4	0.0	0.006071598503699812	1.72980014350422E-5
34-35	8.07240066968636E-5	2.2487401865554858E-4	0.0	0.010228884848588287	2.3064001913389597E-5
36-37	8.07240066968636E-5	2.652360220039804E-4	0.0	0.016715635386729114	2.3064001913389597E-5
38-39	8.07240066968636E-5	2.940660243957174E-4	0.0	0.033246758758151106	2.3064001913389597E-5
40-41	8.07240066968636E-5	3.517260291791914E-4	0.0	0.05075233621041381	2.3064001913389597E-5
42-43	9.802200813190578E-5	3.690240306142336E-4	0.0	0.06267642519963623	2.3064001913389597E-5
44-45	1.0378800861025319E-4	4.03620033484318E-4	0.0	0.07710295839646142	2.3064001913389597E-5
46-47	1.095540090886006E-4	4.3821603635440235E-4	0.0	0.0930113537162219	2.8830002391736996E-5
48-49	1.15320009566948E-4	4.3821603635440235E-4	0.0	0.10833738298766929	4.6128003826779195E-5
50-51	1.268520105236428E-4	4.8434404018118157E-4	0.0	0.12239489215388026	4.6128003826779195E-5
52-53	1.268520105236428E-4	5.074080420945712E-4	0.0	0.15543407489481087	4.6128003826779195E-5
54-55	1.326180110019902E-4	5.074080420945712E-4	0.0	0.1953520962064099	4.6128003826779195E-5
56-57	1.4991601243703238E-4	5.074080420945712E-4	0.0	0.23608312358545594	4.6128003826779195E-5
58-59	1.72980014350422E-4	5.131740425729186E-4	0.0	0.2618398477222338	4.6128003826779195E-5
60-61	1.72980014350422E-4	5.362380444863082E-4	0.0	0.2804409652653825	4.6128003826779195E-5
62-63	1.72980014350422E-4	5.420040449646556E-4	0.0	0.29492515846699113	4.6128003826779195E-5
64-65	1.72980014350422E-4	5.881320487914348E-4	0.0	0.30251898109697467	4.6128003826779195E-5
66-67	1.72980014350422E-4	5.881320487914348E-4	0.0	0.30984756770495425	4.6128003826779195E-5
68-69	1.72980014350422E-4	6.111960507048243E-4	0.0	0.3171242603086286	4.6128003826779195E-5
70-71	1.72980014350422E-4	6.342600526182139E-4	0.0	0.3226826847697555	4.6128003826779195E-5
72-73	1.845120153071168E-4	6.342600526182139E-4	0.0	0.326707353103642	4.6128003826779195E-5
74-75	1.960440162638116E-4	6.342600526182139E-4	0.0	0.32980946136099293	4.6128003826779195E-5
76-77	1.960440162638116E-4	6.457920535749087E-4	0.0	0.3334593396637868	4.6128003826779195E-5
78-79	1.960440162638116E-4	6.573240545316036E-4	0.0	0.335852229862301	4.6128003826779195E-5
80-81	1.960440162638116E-4	6.573240545316036E-4	0.0	0.3365556819206594	4.6128003826779195E-5
82-83	1.960440162638116E-4	6.688560554882983E-4	0.0	0.3371438139694508	4.6128003826779195E-5
84-85	2.0757601722050639E-4	6.688560554882983E-4	0.0	0.3373513899866713	4.6128003826779195E-5
86-87	2.0757601722050639E-4	6.91920057401688E-4	0.0	0.3374378799938465	4.6128003826779195E-5
88	2.0757601722050639E-4	6.91920057401688E-4	0.0	0.3374378799938465	4.6128003826779195E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13330	0.0	49.57124	1
GTATCAA	24145	0.0	36.958214	1
ATCAACG	29345	0.0	29.961092	3
TCAACGC	29475	0.0	29.900377	4
CAACGCA	29780	0.0	29.577686	5
TATCAAC	30370	0.0	29.159374	2
AACGCAG	30450	0.0	28.947052	6
CGCAGAG	34470	0.0	25.53426	8
ACGCAGA	34485	0.0	25.523153	7
GCAGAGT	40130	0.0	21.90117	9
GTGGTAT	6525	0.0	20.604933	1
GAGTACT	25160	0.0	20.347624	12-13
TGGTATC	6270	0.0	19.887136	2
GTACATG	27295	0.0	19.671753	1
TACATGG	26200	0.0	19.635952	2
CAGAGTA	39400	0.0	19.385422	10-11
AGAGTAC	36810	0.0	19.021727	10-11
ACATGGG	26820	0.0	18.59692	3
GTACTTT	27850	0.0	18.352299	14-15
AGTACTT	26730	0.0	17.915316	12-13
>>END_MODULE