FastQCFastQC Report
Fri 27 May 2016
SRR2138634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7595767
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.049.1927721
GTATCAA205400.036.779911
ATCAACG247200.029.9008183
TCAACGC248600.029.8009094
CAACGCA252500.029.3381065
AACGCAG256700.028.9872686
TATCAAC258950.028.809272
ACGCAGA293650.025.2813437
CGCAGAG295400.025.1315738
GTGGTAT56500.021.4998991
GCAGAGT347650.021.3050529
GAGTACT209350.020.8054512-13
GTACATG235100.020.1017131
TGGTATC55700.019.8821322
CAGAGTA335500.019.56964510-11
TACATGG232350.019.508312
ACATGGG234850.019.0264853
AGAGTAC310500.018.94093910-11
GTACTTT234900.018.52491214-15
AGTACTT225300.017.54213512-13
Produced by FastQC (version 0.11.5)