##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7595767
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82985023105633	33.0	33.0	33.0	33.0	33.0
2	32.8263531516962	33.0	33.0	33.0	33.0	33.0
3	32.8227425880757	33.0	33.0	33.0	33.0	33.0
4	36.77718708327941	37.0	37.0	37.0	37.0	37.0
5	36.7903051791873	37.0	37.0	37.0	37.0	37.0
6	36.795520978987376	37.0	37.0	37.0	37.0	37.0
7	36.78208270474858	37.0	37.0	37.0	37.0	37.0
8	36.77909274994875	37.0	37.0	37.0	37.0	37.0
9	36.779743770444774	37.0	37.0	37.0	37.0	37.0
10-11	36.759301463565166	37.0	37.0	37.0	37.0	37.0
12-13	36.74330485650758	37.0	37.0	37.0	37.0	37.0
14-15	39.08370609577676	40.0	40.0	40.0	37.0	40.0
16-17	39.03611228464486	40.0	40.0	40.0	37.0	40.0
18-19	39.06835339999239	40.0	40.0	40.0	37.0	40.0
20-21	39.08279328203587	40.0	40.0	40.0	37.0	40.0
22-23	39.116041003115214	40.0	40.0	40.0	37.0	40.0
24-25	39.11838758751312	40.0	40.0	40.0	37.0	40.0
26-27	39.0398261221618	40.0	40.0	40.0	37.0	40.0
28-29	38.9747386205596	40.0	40.0	40.0	37.0	40.0
30-31	38.902994908180034	40.0	40.0	40.0	37.0	40.0
32-33	38.87096685662016	40.0	40.0	40.0	37.0	40.0
34-35	38.851083182561005	40.0	40.0	40.0	37.0	40.0
36-37	38.8169785523209	40.0	37.0	40.0	37.0	40.0
38-39	38.77222980012148	40.0	37.0	40.0	37.0	40.0
40-41	38.73053861462725	40.0	37.0	40.0	37.0	40.0
42-43	38.66611282241452	40.0	37.0	40.0	37.0	40.0
44-45	38.58236005671482	40.0	37.0	40.0	37.0	40.0
46-47	38.50271738775733	40.0	37.0	40.0	37.0	40.0
48-49	38.46009922002196	40.0	37.0	40.0	37.0	40.0
50-51	38.412845715067476	40.0	37.0	40.0	37.0	40.0
52-53	38.34019519059239	40.0	37.0	40.0	37.0	40.0
54-55	38.27238109586841	40.0	37.0	40.0	37.0	40.0
56-57	38.169221029313334	40.0	37.0	40.0	37.0	40.0
58-59	38.04884922111631	40.0	37.0	40.0	37.0	40.0
60-61	37.881930540198184	40.0	37.0	40.0	35.0	40.0
62-63	37.695055017622046	38.5	37.0	40.0	33.0	40.0
64-65	37.46871049643022	37.0	37.0	40.0	33.0	40.0
66-67	37.232864742844576	37.0	37.0	40.0	33.0	40.0
68-69	36.98676177517683	37.0	37.0	40.0	33.0	40.0
70-71	36.72055244225645	37.0	37.0	40.0	33.0	40.0
72-73	36.468344703514894	37.0	37.0	38.5	33.0	40.0
74-75	36.22279630207663	37.0	37.0	37.0	33.0	40.0
76-77	34.601744055471094	35.0	33.0	37.0	30.0	37.0
78-79	35.82578021680372	37.0	37.0	37.0	33.0	37.0
80-81	35.932437863745264	37.0	37.0	37.0	33.0	37.0
82-83	35.85964603075418	37.0	37.0	37.0	33.0	37.0
84-85	35.733808494692994	37.0	37.0	37.0	33.0	37.0
86-87	35.619444636652396	37.0	37.0	37.0	33.0	37.0
88-89	35.54374912806818	37.0	37.0	37.0	33.0	37.0
90-91	35.47567522565802	37.0	33.0	37.0	33.0	37.0
92-93	35.40102079717483	37.0	33.0	37.0	33.0	37.0
94-95	35.35498112600553	37.0	33.0	37.0	33.0	37.0
96-97	35.31941042962311	37.0	33.0	37.0	33.0	37.0
98-99	35.27630876899515	37.0	33.0	37.0	33.0	37.0
100	35.21265056459163	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	14.0
21	84.0
22	377.0
23	1209.0
24	2849.0
25	6110.0
26	11272.0
27	18701.0
28	28387.0
29	41212.0
30	57457.0
31	78232.0
32	105049.0
33	144918.0
34	209445.0
35	327391.0
36	660116.0
37	2131116.0
38	3616445.0
39	155380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82021381119247	20.199948207995323	14.50380192020108	23.47603606061113
2	14.568943465485448	20.878326046599376	41.916333136600954	22.636397351314226
3	17.978445234752776	26.156280144804334	30.590382968941743	25.274891651501147
4	12.283275257424512	18.26840089032903	39.13944077277716	30.308883079469307
5	13.243867740927953	37.739405785399505	35.53447343236383	13.482253041308706
6	29.582489826241382	38.01838576670401	18.91657550843779	13.482548898616823
7	26.237560999435605	32.92629960871628	23.08692459892464	17.74921479292348
8	24.383251882265476	36.51575936965944	21.181652886403704	17.919335861671374
9	25.595821988747154	16.765193034488814	21.39161193333076	36.24737304343327
10-11	24.0369932358378	26.888515669319503	28.909397826447282	20.16509326839541
12-13	24.972678861792364	24.534059562385206	28.968825663030472	21.524435912791954
14-15	22.133189446174427	25.89754267080599	26.946771800662134	25.02249608235745
16-17	21.439217395688942	29.015404237649733	27.57196870309476	21.973409663566564
18-19	21.34501361087037	28.064539104477532	29.57746070936615	21.012986575285947
20-21	22.284959265757387	27.190914151829144	29.514735203560623	21.009391378852847
22-23	21.995348206756812	27.132340166939745	29.357093102552284	21.515218523751155
24-25	21.706085001696472	27.265960295616274	29.469485575238036	21.55846912744921
26-27	21.666367984819935	27.465047635362172	29.32508663969745	21.54349774012044
28-29	21.485532715048322	27.55103568377789	29.403041779709078	21.560389821464714
30-31	21.88728271633242	27.33264050679889	29.294389643169687	21.485687133698995
32-33	21.67781842062658	27.539505027527962	29.178688244874813	21.603988306970646
34-35	21.7524129540245	27.67308711945774	29.160489586526232	21.414010339991528
36-37	21.793076343236162	27.54459997266794	29.050423663580226	21.61190002051567
38-39	21.66056753506296	27.674080249194017	29.13495088154281	21.530401334200217
40-41	21.931400302333472	27.497197770910887	28.99826873980408	21.57313318695156
42-43	21.82381224961718	27.645017817558543	28.75486079537495	21.77630913744933
44-45	21.947383617874674	27.845988798103598	28.479148660239982	21.727478923781746
46-47	22.022069553693164	27.782043736645733	28.169361428884876	22.02652528077623
48-49	22.01073183481915	27.90277052978768	28.112096266494007	21.974401368899162
50-51	21.975240319631254	28.019336896850117	28.0053895956634	22.000033187855234
52-53	22.12540790555654	27.97389658233517	27.850164290202034	22.050531221906265
54-55	22.10975508038283	27.88489612078986	27.8911996819825	22.11414911684481
56-57	22.21020462908248	27.98642771616775	27.76574151144055	22.03762614330923
58-59	22.082695078319716	28.078884896759966	27.84786282423997	21.990557200680346
60-61	22.164676085296644	28.0632902769743	27.810966640992525	21.96106699673653
62-63	22.087683283277276	28.119149633643815	27.7291038679325	22.064063215146405
64-65	22.09300542105898	28.108201139900135	27.695840511907953	22.102952927132932
66-67	22.166262827594263	28.07370212402721	27.574677635742383	22.18535741263614
68-69	22.151865267264633	28.031365414534715	27.653608469495968	22.16316084870469
70-71	22.098909445221107	27.785627613670062	27.72314917361124	22.392313767497594
72-73	22.199437418790993	27.845900779911613	27.685771419190136	22.268890382107255
74-75	22.08264340995342	28.005147769510153	27.63895885138489	22.27324996915154
76-77	22.175347427571992	27.98498407078273	27.535367128222926	22.304301373422348
78-79	22.154424363791673	27.991781638372533	27.5029861231238	22.350807874711997
80-81	22.096148867720167	28.035315487213936	27.544212059035043	22.324323586030854
82-83	22.244561559949844	27.940423297246404	27.625765358062264	22.189249784741488
84-85	22.357943738719907	27.917186538625664	27.54547691486965	22.17939280778478
86-87	22.129608873777606	27.95138946679766	27.635563731481877	22.283437927942856
88-89	22.2026142188963	27.933934590181845	27.5663179986798	22.29713319224205
90-91	22.23623738169687	27.923120973431658	27.62964480420522	22.210996840666247
92-93	22.25767417331087	27.92823287617991	27.611200795026125	22.202892155483095
94-95	22.077866039297124	28.02085700811563	27.67984689217168	22.221430060415575
96-97	22.20340409417012	28.07206754797818	27.764287396905075	21.960240960946624
98-99	23.289500560217615	30.017460674810632	27.891867190778886	18.801171574192864
100	22.237918862105822	30.999724323158766	21.811107164205527	24.95124965052989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1547.0
1	1455.0
2	2081.5
3	3046.0
4	4428.5
5	7433.5
6	9757.0
7	11059.0
8	11859.0
9	11613.5
10	10741.0
11	10122.5
12	11556.5
13	15804.5
14	25067.0
15	37112.0
16	46466.5
17	51879.5
18	52860.5
19	49692.0
20	44570.0
21	39406.5
22	36455.0
23	37516.0
24	42679.0
25	51897.0
26	64651.5
27	79283.5
28	94040.0
29	110105.5
30	125829.5
31	141027.5
32	158783.5
33	176629.0
34	194349.5
35	211238.0
36	228326.0
37	245643.5
38	256829.0
39	264989.5
40	276359.0
41	286515.5
42	293454.0
43	300071.5
44	306044.0
45	312105.0
46	316167.5
47	317217.5
48	314491.0
49	307680.0
50	299554.0
51	288942.5
52	274772.5
53	258993.5
54	243264.0
55	224912.0
56	205396.0
57	184466.5
58	162666.0
59	142094.0
60	119050.5
61	95921.5
62	75758.0
63	58126.5
64	42920.0
65	31140.0
66	22697.0
67	16873.0
68	12702.5
69	9137.0
70	6274.0
71	4292.5
72	2905.0
73	1896.0
74	1223.5
75	769.0
76	510.5
77	339.5
78	212.5
79	138.0
80	91.5
81	53.5
82	38.5
83	28.5
84	20.5
85	15.0
86	10.0
87	7.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001816801384244672
4	0.010821816940935656
5	0.017628239518142144
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0042335960006410316
26-27	0.04162901494264963
28-29	0.08710018517766883
30-31	0.0831597485710381
32-33	0.03546791544745019
34-35	0.0033393879617449255
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	9.938037048860147E-5
56-57	3.1466225262272775E-4
58-59	1.4412304764614277E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.0259557405557654E-5
72-73	0.012875824571466929
74-75	0.027071617039983238
76-77	0.005591879938847635
78-79	0.0
80-81	0.0
82-83	0.006454350411464839
84-85	0.044286299509015456
86-87	0.041673247008980806
88-89	0.011474406295777248
90-91	2.153766502718095E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	76.0
20-21	355.0
22-23	952.0
24-25	1688.0
26-27	3111.0
28-29	5839.0
30-31	13655.0
32-33	18301.0
34-35	24831.0
36-37	35963.0
38-39	43247.0
40-41	46810.0
42-43	56220.0
44-45	67321.0
46-47	66162.0
48-49	57181.0
50-51	50012.0
52-53	47840.0
54-55	51376.0
56-57	53062.0
58-59	53254.0
60-61	52417.0
62-63	52096.0
64-65	54852.0
66-67	58530.0
68-69	57219.0
70-71	55054.0
72-73	53753.0
74-75	54212.0
76-77	55186.0
78-79	57400.0
80-81	58800.0
82-83	56305.0
84-85	57017.0
86-87	59490.0
88-89	63647.0
90-91	66585.0
92-93	68716.0
94-95	82716.0
96-97	241379.0
98-99	463943.0
100-101	5129194.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.4570282293311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36995803266421	73.75094082156862
2	8.079646520762914	13.486065755326546
3	1.9932069946379316	4.990413972551942
4	0.7431107397915009	2.4807125595319364
5	0.3235622143476358	1.3501770428377762
6	0.17211227156033396	0.8618387223735055
7	0.09139659316925829	0.5339381639334035
8	0.061149520522980796	0.40826858083971757
9	0.03897729491450716	0.2927636281784689
>10	0.12422200585874368	1.5704889452829152
>50	0.0020369656430206337	0.11526454971874886
>100	5.427628968952854E-4	0.09011655282710204
>500	2.6027791295144162E-5	0.012533606191236732
>1k	5.205543871538962E-5	0.05647709883802096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3165227422062841E-5	0.0	0.0	0.0
3	0.0	1.3165227422062841E-5	0.0	2.6330454844125682E-5	0.0
4	0.0	5.2660909688251363E-5	0.0	2.6330454844125682E-5	0.0
5	0.0	6.582613711031421E-5	0.0	2.6330454844125682E-5	0.0
6	0.0	6.582613711031421E-5	0.0	2.6330454844125682E-5	1.3165227422062841E-5
7	0.0	7.899136453237705E-5	0.0	2.6330454844125682E-5	1.3165227422062841E-5
8	0.0	1.3165227422062842E-4	0.0	2.6330454844125682E-5	1.3165227422062841E-5
9	0.0	3.028002307074453E-4	0.0	2.6330454844125682E-5	1.3165227422062841E-5
10-11	0.0	3.028002307074453E-4	0.0	3.2913068555157104E-5	1.3165227422062841E-5
12-13	0.0	3.028002307074453E-4	0.0	3.9495682266188526E-5	1.3165227422062841E-5
14-15	0.0	3.3571329926260245E-4	0.0	3.9495682266188526E-5	1.3165227422062841E-5
16-17	0.0	3.488785266846653E-4	0.0	3.9495682266188526E-5	1.3165227422062841E-5
18-19	0.0	3.8837420895085383E-4	0.0	7.240875082134562E-5	3.2913068555157104E-5
20-21	0.0	4.081220500839481E-4	0.0	9.873920566547131E-5	5.2660909688251363E-5
22-23	0.0	4.8711341461632517E-4	0.0	1.1190443308753414E-4	5.9243523399282786E-5
24-25	0.0	5.726873928597336E-4	0.0	1.25069660509597E-4	6.582613711031421E-5
26-27	0.0	6.056004614148906E-4	0.0	1.843131839088798E-4	6.582613711031421E-5
28-29	0.0	6.319309162590163E-4	0.0	4.607829597721994E-4	6.582613711031421E-5
30-31	0.0	7.570005767686134E-4	0.0	0.001270444446229064	6.582613711031421E-5
32-33	0.0	7.767484179017077E-4	0.0	0.0028897674191427935	9.873920566547131E-5
34-35	0.0	9.149833058333675E-4	0.0	0.0050883603986272875	1.0532181937650273E-4
36-37	0.0	0.0010598008074760587	0.0	0.008182188842812056	1.0532181937650273E-4
38-39	0.0	0.0010927138760312158	0.0	0.016041829613783573	1.0532181937650273E-4
40-41	0.0	0.0011980356954077185	0.0	0.024375418571949352	1.1848704679856557E-4
42-43	0.0	0.0012243661502518441	0.0	0.030602571142585078	1.1848704679856557E-4
44-45	0.0	0.0013494358107614412	0.0	0.03837663793531318	1.1848704679856557E-4
46-47	0.0	0.0014481750164269125	0.0	0.04638767882163842	1.3165227422062842E-4
48-49	0.0	0.0014481750164269125	0.0	0.054800259144336574	1.4481750164269125E-4
50-51	0.0	0.0016785664963130122	0.0	0.06315359594363545	1.4481750164269125E-4
52-53	0.0	0.0016851491100240436	0.0	0.07922175601226314	1.4481750164269125E-4
54-55	0.0	0.0016851491100240436	0.0	0.09866021430093894	1.4481750164269125E-4
56-57	0.0	0.0016983143374461065	0.0	0.11898732544060395	1.5140011535372266E-4
58-59	0.0	0.001737810019712295	0.0	0.13687228689347633	1.579827290647541E-4
60-61	0.0	0.0017641404745564206	0.0	0.14590363290501143	1.7114795648681693E-4
62-63	0.0	0.0018036361568226093	0.0	0.15636998870555138	1.7114795648681693E-4
64-65	0.0	0.0019747841133094263	0.0	0.16165582751550964	1.7114795648681693E-4
66-67	0.0	0.0019945319544425206	0.0	0.16639530938745226	1.7114795648681693E-4
68-69	0.0	0.002047192864130772	0.0	0.171371765352992	2.0406102504197404E-4
70-71	0.0	0.002093271160107992	0.0	0.17605858631524637	2.303914798860997E-4
72-73	0.0	0.002113019001241086	0.0	0.18003448499670935	2.7646977586331966E-4
74-75	0.0	0.0021788451383514	0.0	0.18300982639409555	2.7646977586331966E-4
76-77	0.0	0.002284166957727903	0.0	0.18597858517777072	2.7646977586331966E-4
78-79	0.0	0.0023434104811271856	0.0	0.1878217170168595	2.896350032853825E-4
80-81	0.0	0.0023434104811271856	0.0	0.18846023054682956	3.2254807184053957E-4
82-83	0.0	0.0023565757085492484	0.0	0.18883543952835835	3.4229591297363387E-4
84-85	0.0	0.0024224018456595626	0.0	0.18893417873402382	3.4229591297363387E-4
86-87	0.0	0.002461897527925751	0.0	0.18896050918886795	3.4229591297363387E-4
88	0.0	0.002580384574724317	0.0	0.18896050918886795	3.4229591297363387E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11640	0.0	49.192772	1
GTATCAA	20540	0.0	36.77991	1
ATCAACG	24720	0.0	29.900818	3
TCAACGC	24860	0.0	29.800909	4
CAACGCA	25250	0.0	29.338106	5
AACGCAG	25670	0.0	28.987268	6
TATCAAC	25895	0.0	28.80927	2
ACGCAGA	29365	0.0	25.281343	7
CGCAGAG	29540	0.0	25.131573	8
GTGGTAT	5650	0.0	21.499899	1
GCAGAGT	34765	0.0	21.305052	9
GAGTACT	20935	0.0	20.80545	12-13
GTACATG	23510	0.0	20.101713	1
TGGTATC	5570	0.0	19.882132	2
CAGAGTA	33550	0.0	19.569645	10-11
TACATGG	23235	0.0	19.50831	2
ACATGGG	23485	0.0	19.026485	3
AGAGTAC	31050	0.0	18.940939	10-11
GTACTTT	23490	0.0	18.524912	14-15
AGTACTT	22530	0.0	17.542135	12-13
>>END_MODULE