FastQCFastQC Report
Fri 27 May 2016
SRR2138633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808015
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130600.054.0029221
GTATCAA236950.037.911971
ATCAACG282200.031.3492553
TCAACGC297500.029.763944
TATCAAC302600.029.3907322
CAACGCA302300.029.3432125
AACGCAG308050.028.7907036
ACGCAGA309650.028.6419377
CGCAGAG353000.025.112518
TGGTATC51700.024.8103472
GTGGTAT54700.024.1510931
GCAGAGT404100.021.8315229
GAGTACT245700.021.0391112-13
GTACATG275950.020.4156631
TACATGG271100.020.1675912
ACATGGG270000.019.8709583
CAGAGTA395750.019.77876510-11
AGAGTAC372900.019.14583810-11
GTACTTT272450.018.76258914-15
AGTACTT263750.018.16183712-13
Produced by FastQC (version 0.11.5)