##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7808015
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89154067967338	33.0	33.0	33.0	33.0	33.0
2	32.94881157374826	33.0	33.0	33.0	33.0	33.0
3	33.617996379361465	33.0	33.0	33.0	33.0	37.0
4	36.780762075892525	37.0	37.0	37.0	37.0	37.0
5	36.79448617862543	37.0	37.0	37.0	37.0	37.0
6	36.79664818779165	37.0	37.0	37.0	37.0	37.0
7	36.788291390321355	37.0	37.0	37.0	37.0	37.0
8	36.77710006960796	37.0	37.0	37.0	37.0	37.0
9	36.78316435099062	37.0	37.0	37.0	37.0	37.0
10-11	36.77761332425719	37.0	37.0	37.0	37.0	37.0
12-13	37.03050826618545	37.0	37.0	37.0	37.0	38.5
14-15	39.09017208599113	40.0	40.0	40.0	37.0	40.0
16-17	39.043036930641144	40.0	40.0	40.0	37.0	40.0
18-19	39.08481310550761	40.0	40.0	40.0	37.0	40.0
20-21	39.107793351151656	40.0	40.0	40.0	37.0	40.0
22-23	39.13775604079957	40.0	40.0	40.0	37.0	40.0
24-25	39.12822452380016	40.0	40.0	40.0	37.0	40.0
26-27	39.04876202326247	40.0	40.0	40.0	37.0	40.0
28-29	38.985794112000036	40.0	40.0	40.0	37.0	40.0
30-31	38.913230194990305	40.0	40.0	40.0	37.0	40.0
32-33	38.88122508945378	40.0	40.0	40.0	37.0	40.0
34-35	38.8624767295186	40.0	40.0	40.0	37.0	40.0
36-37	38.826474216907144	40.0	37.0	40.0	37.0	40.0
38-39	38.78419760469256	40.0	37.0	40.0	37.0	40.0
40-41	38.742553609652354	40.0	37.0	40.0	37.0	40.0
42-43	38.67403279023341	40.0	37.0	40.0	37.0	40.0
44-45	38.591270243223846	40.0	37.0	40.0	37.0	40.0
46-47	38.51152910955031	40.0	37.0	40.0	37.0	40.0
48-49	38.470428456372304	40.0	37.0	40.0	37.0	40.0
50-51	38.42185443005282	40.0	37.0	40.0	37.0	40.0
52-53	38.35718326914153	40.0	37.0	40.0	37.0	40.0
54-55	38.29313369868852	40.0	37.0	40.0	37.0	40.0
56-57	38.19097252974572	40.0	37.0	40.0	37.0	40.0
58-59	38.07284333814698	40.0	37.0	40.0	37.0	40.0
60-61	37.9027227730834	40.0	37.0	40.0	35.0	40.0
62-63	37.715258013128434	38.5	37.0	40.0	33.0	40.0
64-65	37.48831679720759	37.0	37.0	40.0	33.0	40.0
66-67	37.250463120885364	37.0	37.0	40.0	33.0	40.0
68-69	37.00196844392079	37.0	37.0	40.0	33.0	40.0
70-71	36.73486603976016	37.0	37.0	40.0	33.0	40.0
72-73	36.4754860861287	37.0	37.0	37.0	33.0	40.0
74-75	36.04021896839056	37.0	37.0	37.0	33.0	40.0
76-77	34.83452799220673	37.0	33.0	37.0	30.0	37.0
78-79	35.883632976533924	37.0	37.0	37.0	33.0	37.0
80-81	35.95949237348568	37.0	37.0	37.0	33.0	37.0
82-83	35.88186787399327	37.0	37.0	37.0	33.0	37.0
84-85	35.760540091971485	37.0	37.0	37.0	33.0	37.0
86-87	35.656972639893795	37.0	37.0	37.0	33.0	37.0
88-89	35.58585496330785	37.0	37.0	37.0	33.0	37.0
90-91	35.521610582448716	37.0	35.0	37.0	33.0	37.0
92-93	35.45340778184804	37.0	33.0	37.0	33.0	37.0
94-95	35.41186916939897	37.0	33.0	37.0	33.0	37.0
96-97	35.373932207788165	37.0	33.0	37.0	33.0	37.0
98-99	35.32588119898085	37.0	33.0	37.0	33.0	37.0
100	35.2498404499421	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	30.0
21	73.0
22	356.0
23	1105.0
24	2780.0
25	5827.0
26	10983.0
27	18286.0
28	27808.0
29	40716.0
30	56622.0
31	78505.0
32	106751.0
33	146093.0
34	211233.0
35	326403.0
36	648143.0
37	2120051.0
38	3796901.0
39	209341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.0195883734104	0.0	24.695264433307432	29.285147193282175
2	15.129654374111723	23.692208109400276	38.57306481559631	22.605072700891693
3	16.721215311531807	24.50914544725662	32.975545966743134	25.794093274468445
4	13.024772860368497	23.17079137789077	37.300098423330994	26.504337338409744
5	17.419900182570363	36.32489301386872	32.03932493669344	14.215881866867482
6	27.990686493302075	37.130141271501145	20.274640865828257	14.604531369368527
7	26.27443722892438	33.16052799591189	22.12882531603743	18.436209459126296
8	24.23140836691528	32.090217552092305	22.12737808521116	21.550995995781257
9	24.101900418992535	20.544555818604344	23.541271373069854	31.81227238933327
10-11	24.678525848118888	26.16685546838729	28.36072932749233	20.793889356001493
12-13	25.091883148277766	24.37919240677688	28.358391985671133	22.17053245927422
14-15	22.48711100068327	26.70930447751445	26.774846103651186	24.02873841815109
16-17	21.273198117575337	29.01585101975342	27.76768487253162	21.94326599013962
18-19	21.316908668056776	27.883502499291435	29.817682168305815	20.981906664345974
20-21	22.285721275048058	27.100115262713366	29.771496265504737	20.842667196733835
22-23	22.102444709256762	26.863968565452957	29.552125974892558	21.481460750397726
24-25	21.679990770274056	27.175841589511524	29.747333689889793	21.396833950324627
26-27	21.57575855481414	27.404476816561218	29.550349682581412	21.46941494604323
28-29	21.555759788627114	27.39575263797426	29.594661804085842	21.45382576931278
30-31	21.824732088069652	27.203769410716117	29.619195300389528	21.352303200824704
32-33	21.610798567678724	27.464107537955773	29.446958407940098	21.4781354864254
34-35	21.783235537216193	27.394330477580965	29.371482567125828	21.450951418077015
36-37	21.73665275139564	27.414592095105938	29.362173376587315	21.48658177691111
38-39	21.57378198957778	27.646093423929468	29.363720145256565	21.416404441236185
40-41	21.923606975754996	27.3607564702418	29.141452082583513	21.57418447141969
42-43	21.823153395991262	27.4315123537301	29.060776610516942	21.6845576397617
44-45	21.89700508614398	27.782182937761334	28.65235187490857	21.668460101186113
46-47	22.01532184365016	27.654665190907846	28.33098010717723	21.999032858264762
48-49	21.945545985144843	27.842674708447962	28.292390868923533	21.919388437483665
50-51	22.012111264297584	28.06752499780049	27.960521982509167	21.95984175539276
52-53	22.174220755746713	27.839894132153027	27.904562732048277	22.081322380051986
54-55	22.161846665062562	27.801312955388024	27.947129934266346	22.089710445283067
56-57	22.14356387916364	28.077670564422363	27.80731363421441	21.971451922199588
58-59	22.16400957808719	27.889236812062308	27.89168573081893	22.055067879031576
60-61	22.144167596327552	27.989070791707864	27.8953101818333	21.97145143013128
62-63	22.14431181919365	28.081480179598234	27.767539350573074	22.00666865063504
64-65	22.172885110806646	27.922164104349754	27.701700677697406	22.203250107146193
66-67	22.18268471473722	27.969801515538727	27.67335990575773	22.17415386396631
68-69	22.189624664775064	27.997379048728572	27.745581879000685	22.06741440749568
70-71	22.205842151234506	27.647278026331097	27.775229272838935	22.37165054959546
72-73	22.20457286035403	27.795838362716097	27.739089579039167	22.26049919789071
74-75	22.126256121423506	27.951574524494262	27.6978391477848	22.224330206297434
76-77	22.335625813596906	27.750210802053253	27.53037491916398	22.383788465185862
78-79	22.154704359755602	27.854302636934808	27.685546439336534	22.305446563973057
80-81	22.21256013046233	27.8828179508317	27.608187821117667	22.2964340975883
82-83	22.36820285919685	27.701692597551354	27.672515837578725	22.257588705673072
84-85	22.405106160392677	27.712094240231146	27.681647528166582	22.2011520712096
86-87	22.26203018695361	27.80166763586804	27.65912245452312	22.277179722655227
88-89	22.366576889358186	27.61339960796431	27.650510806122377	22.36951269655513
90-91	22.381192725097048	27.599769344679796	27.73266542680675	22.286372503416402
92-93	22.358079655472	27.66344503057437	27.722490345484097	22.255984968469537
94-95	22.378452954151612	27.561275783444227	27.75995474661162	22.300316515792538
96-97	22.389752500680203	27.839069121185528	27.856633327711293	21.914545050422976
98-99	23.43904434994168	29.934823845578016	27.36840911356732	19.25772269091299
100	22.485079306157065	30.29497872220507	21.77416749124145	25.445774480396416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1643.0
1	1494.5
2	2101.0
3	3119.5
4	4576.0
5	7609.0
6	9943.5
7	11712.5
8	13276.0
9	13572.0
10	12898.5
11	12191.0
12	13616.0
13	17941.0
14	27980.5
15	42352.0
16	54730.0
17	61775.0
18	62052.0
19	57018.0
20	49881.0
21	42409.5
22	37370.0
23	37512.0
24	42226.0
25	51067.0
26	63877.5
27	78706.0
28	93693.0
29	110689.0
30	127911.0
31	144645.5
32	162791.5
33	180464.5
34	198091.5
35	214313.5
36	230997.5
37	248305.5
38	260983.5
39	270605.0
40	281356.0
41	292124.0
42	299481.0
43	306195.5
44	312729.5
45	318349.5
46	322295.0
47	324823.0
48	324969.0
49	319333.5
50	311879.5
51	302688.5
52	288587.5
53	272720.0
54	255670.5
55	234924.5
56	213793.5
57	191866.0
58	169425.5
59	147944.5
60	124617.5
61	101653.0
62	80453.0
63	61901.0
64	46537.0
65	34216.5
66	25526.5
67	19125.5
68	14442.5
69	10396.5
70	7162.0
71	5027.0
72	3392.0
73	2162.0
74	1361.0
75	892.5
76	563.0
77	359.0
78	243.0
79	165.5
80	112.0
81	78.0
82	59.0
83	43.5
84	32.0
85	19.5
86	12.5
87	10.0
88	6.5
89	6.5
90	5.0
91	4.0
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.0491763911826503E-4
2	8.196705564730601E-4
3	0.003906242495691927
4	0.011821186306634913
5	0.014882143540963998
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.561470488978313E-5
20-21	5.1230692512807186E-5
22-23	3.522374249318004E-4
24-25	0.007648146813672504
26-27	0.04687075103666696
28-29	0.08791980923568299
30-31	0.07825185660424297
32-33	0.03017989351571515
34-35	0.002359999174645097
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.8752493567001055E-6
54-55	1.9393281197729046E-4
56-57	2.652809691970399E-4
58-59	7.03712236866161E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	7.272677025140553E-6
68-69	7.339950004664537E-5
70-71	0.0011927453523492009
72-73	0.014248718026542087
74-75	0.022837985205331368
76-77	0.0036865196923370814
78-79	4.615026063359693E-5
80-81	4.895343766580063E-4
82-83	0.009774358348703414
84-85	0.04264846237500618
86-87	0.03612448275488865
88-89	0.008595823565044841
90-91	1.0672394513436914E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	119.0
20-21	439.0
22-23	1022.0
24-25	2306.0
26-27	3540.0
28-29	7137.0
30-31	14105.0
32-33	19219.0
34-35	26820.0
36-37	37263.0
38-39	43895.0
40-41	47831.0
42-43	60153.0
44-45	72891.0
46-47	71328.0
48-49	60773.0
50-51	53649.0
52-53	52654.0
54-55	56230.0
56-57	57307.0
58-59	56960.0
60-61	55740.0
62-63	56396.0
64-65	59646.0
66-67	62939.0
68-69	63001.0
70-71	61393.0
72-73	60698.0
74-75	62680.0
76-77	63023.0
78-79	65358.0
80-81	66458.0
82-83	64803.0
84-85	66379.0
86-87	69738.0
88-89	74374.0
90-91	76357.0
92-93	79739.0
94-95	101724.0
96-97	362169.0
98-99	1373496.0
100-101	4116263.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.80084124350556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.50912352776733	76.79958097651294
2	7.640342411025538	13.110956125088494
3	1.6909451395039858	4.3525354639817655
4	0.5782555859667675	1.9845926291881963
5	0.24639990002780937	1.0570659352350857
6	0.12531198488503253	0.6451124232617548
7	0.06569244552303762	0.39455269624538514
8	0.040078118934299196	0.2750989056016112
9	0.023882797926624316	0.18442477380177152
>10	0.07822544366057543	1.0001361406806462
>50	0.0012757208537480208	0.0723611274129224
>100	4.2238409162577683E-4	0.08808055933667572
>500	2.2269927775547733E-5	0.013604743187527635
>1k	2.226990583322726E-5	0.021897500465206148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.2807352444891563E-5
5	0.0	0.0	0.0	0.0	1.2807352444891563E-5
6	0.0	1.2807352444891563E-5	0.0	1.2807352444891563E-5	1.2807352444891563E-5
7	0.0	1.2807352444891563E-5	0.0	2.5614704889783125E-5	1.2807352444891563E-5
8	0.0	2.5614704889783125E-5	0.0	2.5614704889783125E-5	1.2807352444891563E-5
9	0.0	5.122940977956625E-5	0.0	3.842205733467469E-5	1.2807352444891563E-5
10-11	1.2807352444891563E-5	5.122940977956625E-5	0.0	6.403676222445782E-5	2.5614704889783125E-5
12-13	1.2807352444891563E-5	5.122940977956625E-5	0.0	7.04404384469036E-5	3.201838111222891E-5
14-15	1.2807352444891563E-5	1.024588195591325E-4	0.0	9.605514333668673E-5	4.4825733557120475E-5
16-17	1.2807352444891563E-5	1.0886249578157828E-4	0.0	1.2166984822646986E-4	5.122940977956625E-5
18-19	1.2807352444891563E-5	1.1526617200402406E-4	0.0	1.47284553116253E-4	5.763308600201204E-5
20-21	1.2807352444891563E-5	1.1526617200402406E-4	0.0	1.6009190556114455E-4	6.403676222445782E-5
22-23	1.2807352444891563E-5	1.2807352444891564E-4	0.0	1.8570661045092765E-4	8.324779089179517E-5
24-25	1.2807352444891563E-5	1.3447720067136142E-4	0.0	1.9851396289581922E-4	1.024588195591325E-4
26-27	1.2807352444891563E-5	1.408808768938072E-4	0.0	2.4333969645293972E-4	1.024588195591325E-4
28-29	1.2807352444891563E-5	1.408808768938072E-4	0.0	4.290463069038674E-4	1.024588195591325E-4
30-31	1.2807352444891563E-5	1.6649558178359034E-4	0.0	8.965146711424094E-4	1.024588195591325E-4
32-33	1.2807352444891563E-5	1.6649558178359034E-4	0.0	0.001933910219178626	1.024588195591325E-4
34-35	1.2807352444891563E-5	1.8570661045092765E-4	0.0	0.0032530675210024574	1.1526617200402406E-4
36-37	1.2807352444891563E-5	2.04917639118265E-4	0.0	0.005001271129730155	1.1526617200402406E-4
38-39	1.2807352444891563E-5	2.04917639118265E-4	0.0	0.007703622495602275	1.1526617200402406E-4
40-41	1.2807352444891563E-5	2.433396964529397E-4	0.0	0.010719753996374239	1.2166984822646986E-4
42-43	1.2807352444891563E-5	2.94569106232506E-4	0.0	0.014100895041825611	1.2807352444891564E-4
44-45	1.2807352444891563E-5	3.4579851601207224E-4	0.0	0.01789827504173596	1.408808768938072E-4
46-47	1.2807352444891563E-5	3.4579851601207224E-4	0.0	0.02172126974653609	1.6649558178359034E-4
48-49	1.2807352444891563E-5	3.4579851601207224E-4	0.0	0.02564031959467291	1.9211028667337344E-4
50-51	1.2807352444891563E-5	4.290463069038673E-4	0.0	0.029444103270805703	1.9851396289581922E-4
52-53	1.2807352444891563E-5	4.4185365934875894E-4	0.0	0.03319665753715893	2.2412866778560235E-4
54-55	1.2807352444891563E-5	4.482573355712047E-4	0.0	0.03739106546286092	2.433396964529397E-4
56-57	1.2807352444891563E-5	4.6106468801609626E-4	0.0	0.041873638818572964	2.497433726753855E-4
58-59	1.2807352444891563E-5	4.6106468801609626E-4	0.0	0.046938946710527577	2.6255072512027704E-4
60-61	1.2807352444891563E-5	4.6746836423854207E-4	0.0	0.05134467595157028	2.6895440134272285E-4
62-63	1.2807352444891563E-5	4.99486745350771E-4	0.0	0.05587847871706189	2.6895440134272285E-4
64-65	1.2807352444891563E-5	5.635235075752288E-4	0.0	0.060303418986771926	2.6895440134272285E-4
66-67	1.2807352444891563E-5	5.635235075752288E-4	0.0	0.06486283645715332	2.6895440134272285E-4
68-69	1.2807352444891563E-5	5.827345362425661E-4	0.0	0.06979366714843657	2.817617537876144E-4
70-71	1.2807352444891563E-5	6.019455649099035E-4	0.0	0.07432106623770574	3.0737645867739754E-4
72-73	1.2807352444891563E-5	6.019455649099035E-4	0.0	0.07780466610271625	3.6500954467940955E-4
74-75	1.2807352444891563E-5	6.147529173547951E-4	0.0	0.0810001005377167	3.7141322090185536E-4
76-77	1.2807352444891563E-5	6.275602697996866E-4	0.0	0.08389456219026219	3.7141322090185536E-4
78-79	1.2807352444891563E-5	6.275602697996866E-4	0.0	0.08555951800809809	3.7141322090185536E-4
80-81	1.2807352444891563E-5	6.275602697996866E-4	0.0	0.08628313342123446	3.778168971243011E-4
82-83	1.2807352444891563E-5	6.275602697996866E-4	0.0	0.08680823487147502	3.842205733467469E-4
84-85	1.2807352444891563E-5	6.339639460221324E-4	0.0	0.08699394148192593	3.906242495691927E-4
86-87	2.5614704889783125E-5	6.403676222445782E-4	0.0	0.08708999662526264	3.9702792579163844E-4
88	2.5614704889783125E-5	6.403676222445782E-4	0.0	0.08708999662526264	3.9702792579163844E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13060	0.0	54.002922	1
GTATCAA	23695	0.0	37.91197	1
ATCAACG	28220	0.0	31.349255	3
TCAACGC	29750	0.0	29.76394	4
TATCAAC	30260	0.0	29.390732	2
CAACGCA	30230	0.0	29.343212	5
AACGCAG	30805	0.0	28.790703	6
ACGCAGA	30965	0.0	28.641937	7
CGCAGAG	35300	0.0	25.11251	8
TGGTATC	5170	0.0	24.810347	2
GTGGTAT	5470	0.0	24.151093	1
GCAGAGT	40410	0.0	21.831522	9
GAGTACT	24570	0.0	21.03911	12-13
GTACATG	27595	0.0	20.415663	1
TACATGG	27110	0.0	20.167591	2
ACATGGG	27000	0.0	19.870958	3
CAGAGTA	39575	0.0	19.778765	10-11
AGAGTAC	37290	0.0	19.145838	10-11
GTACTTT	27245	0.0	18.762589	14-15
AGTACTT	26375	0.0	18.161837	12-13
>>END_MODULE