FastQCFastQC Report
Fri 27 May 2016
SRR2138632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7679226
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124700.049.746441
CTTATAC63900.038.107231
GTATCAA223750.037.13221
ATCAACG269500.030.3037343
TCAACGC272800.029.9681954
CAACGCA275100.029.6514475
TATCAAC277450.029.6362882
AACGCAG281200.029.0781946
TATACAC94450.028.9132823
TTATACA96550.026.1530882
CGCAGAG322000.025.3804448
ACGCAGA323600.025.2152237
GCAGAGT366650.022.2545919
GTGGTAT56300.022.0064181
GAGTACT226500.021.11545212-13
GTACATG241850.020.8991031
TACACAT128650.020.7575445
ACATCTC120900.020.7009818
TACATGG238850.020.6590352
TGGTATC56050.020.1924132
Produced by FastQC (version 0.11.5)