##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7679226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82250541916594	33.0	33.0	33.0	33.0	33.0
2	32.82307188250483	33.0	33.0	33.0	33.0	33.0
3	32.81948949021685	33.0	33.0	33.0	33.0	33.0
4	36.77251014620484	37.0	37.0	37.0	37.0	37.0
5	36.78543345384027	37.0	37.0	37.0	37.0	37.0
6	36.79139538281593	37.0	37.0	37.0	37.0	37.0
7	36.77674455732909	37.0	37.0	37.0	37.0	37.0
8	36.775182160285425	37.0	37.0	37.0	37.0	37.0
9	36.775378143578536	37.0	37.0	37.0	37.0	37.0
10-11	36.75415914312198	37.0	37.0	37.0	37.0	37.0
12-13	36.73857671333022	37.0	37.0	37.0	37.0	37.0
14-15	39.070766051161925	40.0	40.0	40.0	37.0	40.0
16-17	39.020869616286845	40.0	40.0	40.0	37.0	40.0
18-19	39.052523978328026	40.0	40.0	40.0	37.0	40.0
20-21	39.067360273249975	40.0	40.0	40.0	37.0	40.0
22-23	39.09945575268392	40.0	40.0	40.0	37.0	40.0
24-25	39.102606608177155	40.0	40.0	40.0	37.0	40.0
26-27	39.016865022723685	40.0	40.0	40.0	37.0	40.0
28-29	38.94653904293446	40.0	40.0	40.0	37.0	40.0
30-31	38.87006828264706	40.0	40.0	40.0	37.0	40.0
32-33	38.84011507883973	40.0	38.5	40.0	37.0	40.0
34-35	38.81988121442427	40.0	37.0	40.0	37.0	40.0
36-37	38.78204878870011	40.0	37.0	40.0	37.0	40.0
38-39	38.72694597262451	40.0	37.0	40.0	37.0	40.0
40-41	38.68839892294871	40.0	37.0	40.0	37.0	40.0
42-43	38.62475625611423	40.0	37.0	40.0	37.0	40.0
44-45	38.53998213031249	40.0	37.0	40.0	37.0	40.0
46-47	38.45908653275447	40.0	37.0	40.0	37.0	40.0
48-49	38.410035413874425	40.0	37.0	40.0	37.0	40.0
50-51	38.34411212284435	40.0	37.0	40.0	37.0	40.0
52-53	38.2730052205677	40.0	37.0	40.0	37.0	40.0
54-55	38.207366439594026	40.0	37.0	40.0	37.0	40.0
56-57	38.09857934710536	40.0	37.0	40.0	37.0	40.0
58-59	37.97830956099653	40.0	37.0	40.0	37.0	40.0
60-61	37.801098455077565	40.0	37.0	40.0	33.0	40.0
62-63	37.61343355903979	38.5	37.0	40.0	33.0	40.0
64-65	37.39442322601455	37.0	37.0	40.0	33.0	40.0
66-67	37.164339004325214	37.0	37.0	40.0	33.0	40.0
68-69	36.92711122020256	37.0	37.0	40.0	33.0	40.0
70-71	36.668088854293856	37.0	37.0	40.0	33.0	40.0
72-73	36.4252025246995	37.0	37.0	37.0	33.0	40.0
74-75	36.18579840058665	37.0	37.0	37.0	33.0	40.0
76-77	34.55848502897325	35.0	33.0	37.0	30.0	37.0
78-79	35.79702619376776	37.0	37.0	37.0	33.0	37.0
80-81	35.911939758295865	37.0	37.0	37.0	33.0	37.0
82-83	35.843070354295634	37.0	37.0	37.0	33.0	37.0
84-85	35.721956566452704	37.0	37.0	37.0	33.0	37.0
86-87	35.61303372848764	37.0	37.0	37.0	33.0	37.0
88-89	35.537212133091955	37.0	37.0	37.0	33.0	37.0
90-91	35.47130860078259	37.0	33.0	37.0	33.0	37.0
92-93	35.399409663741096	37.0	33.0	37.0	33.0	37.0
94-95	35.354091087268124	37.0	33.0	37.0	33.0	37.0
96-97	35.318914703756974	37.0	33.0	37.0	33.0	37.0
98-99	35.27587298848812	37.0	33.0	37.0	33.0	37.0
100	35.214044553337324	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	30.0
21	116.0
22	409.0
23	1360.0
24	3271.0
25	6708.0
26	12352.0
27	20327.0
28	30822.0
29	43453.0
30	60140.0
31	81757.0
32	109893.0
33	151062.0
34	216830.0
35	336870.0
36	684629.0
37	2202981.0
38	3562316.0
39	153898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36122572769704	20.19578535649296	14.79787155632612	23.645117359483887
2	14.481654791771984	20.559884550864894	41.92326153703511	23.035199120328013
3	17.928185993986006	25.938982069092326	30.699730319502827	25.433101617418842
4	12.331231974515408	18.169388925897636	38.58054416532686	30.918834934260094
5	13.359404742476674	37.53885671930311	35.3674780574395	13.734260480780716
6	29.849310334140444	37.67266388565723	18.590219899765938	13.887805880436389
7	26.417375917833386	33.007740623859746	22.459099393610764	18.115784064696104
8	24.717764524706006	36.22527843300875	20.901533566013033	18.15542347627222
9	25.443579860782844	16.929831209551587	21.169972077915144	36.456616851750425
10-11	24.15490180911462	26.786234706466512	28.443159505918956	20.615703978499916
12-13	25.091565217640422	24.324365502460797	28.651839391105298	21.93222988879348
14-15	22.29702576796151	25.75262142304446	26.50780169772318	25.442551111270852
16-17	21.638267971277315	29.06682913095669	27.13772195270721	22.157180945058784
18-19	21.660646528699637	27.883116084876264	29.195071222021596	21.261166164402507
20-21	22.472162073328462	26.936133855408944	29.214202668753025	21.37750140250957
22-23	22.145374729311087	27.026930584344242	29.073526324480802	21.754168361863872
24-25	21.983127971604546	27.107018761988915	29.198050134395675	21.711803132010868
26-27	21.957284749373116	27.347696180981085	29.093476423507692	21.601542646138107
28-29	21.67495608615639	27.318249457767013	29.2049547743997	21.8018396816769
30-31	21.95694708065606	27.159377229608257	29.202024836856662	21.681650852879024
32-33	21.648300563519996	27.14887590140845	29.36026636274812	21.84255717232344
34-35	21.740918059331214	27.301800594935006	29.16733658689164	21.789944758842143
36-37	21.827817155866995	27.281548735091356	29.12895313312761	21.76168097591404
38-39	21.81635676490425	27.29504874589626	29.079854012777545	21.808740476421946
40-41	21.99593354429225	27.203145051272543	29.014467318119586	21.786454086315622
42-43	22.000642684030037	27.25775133333578	28.883401567067885	21.858204415566302
44-45	21.992750090607057	27.54414866684911	28.540436299036237	21.92266494350759
46-47	22.268601925310122	27.31577826374795	28.048344385235673	22.367275425706254
48-49	22.2108843537415	27.49076988527162	28.03980268962602	22.258543071360858
50-51	22.09311017756351	27.70619775707438	27.967625533732587	22.23306653162952
52-53	22.178795175342085	27.782671112670382	27.954046095237807	22.084487616749726
54-55	22.13358117913604	27.78160411031903	27.754435752804397	22.330378957740532
56-57	22.421634933372257	27.93249407802662	27.507388655006498	22.138482333594624
58-59	22.072083229154476	28.222313269555883	27.813139644285656	21.89246385700399
60-61	22.399872819379897	28.27938535856732	27.38904943939029	21.931692382662494
62-63	22.215802655376056	28.418723759629472	27.305506857329487	22.059966727664985
64-65	22.1783112537064	28.252642739325566	27.408818216531916	22.16022779043612
66-67	22.33447065018985	28.127957418762694	27.322906636892924	22.21466529415453
68-69	22.305331659265796	27.880220408318635	27.532059364766308	22.282388567649257
70-71	22.294077831056853	27.674696635976748	27.548571738495898	22.482653794470504
72-73	22.403042040286138	27.747918989493257	27.458450912242853	22.39058805797775
74-75	22.28061920763375	27.85006625079704	27.45420972612369	22.415104815445517
76-77	22.395115301313247	27.823851959985735	27.315689697430486	22.465343041270533
78-79	22.309607851585987	27.847572154865645	27.34332754549432	22.49949244805405
80-81	22.320182701893675	27.844280193582755	27.40031540700442	22.43522169751915
82-83	22.401727124926747	27.83412112929614	27.48338096398123	22.28077078179588
84-85	22.520509663848426	27.820642999631705	27.35352836521063	22.30531897130924
86-87	22.2916315974519	27.822188252862272	27.43700829667382	22.449171853012007
88-89	22.41457529944377	27.765855503996917	27.388415948947415	22.4311532476119
90-91	22.404642190175032	27.878778545081634	27.37602669396062	22.34055257078271
92-93	22.436300806705514	27.816648097724094	27.409792054780898	22.337259040789494
94-95	22.296280788742433	27.86068258528942	27.471770378837668	22.371266247130478
96-97	22.336945139682836	28.00773860409865	27.50407701524718	22.151239240971343
98-99	23.49730990869172	29.863317891901065	27.65805933980173	18.981312859605488
100	22.455259546193496	30.800848831051198	21.55861523316539	25.185276389589916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2503.0
1	2117.0
2	2349.0
3	3176.0
4	4418.0
5	7234.5
6	9517.0
7	11012.5
8	11982.5
9	11701.0
10	10808.0
11	10318.0
12	11868.5
13	16441.5
14	26200.0
15	38773.0
16	48949.5
17	55232.5
18	56283.0
19	52704.5
20	46986.0
21	41058.5
22	37539.5
23	38455.5
24	43931.5
25	52946.5
26	64873.0
27	78950.5
28	92896.5
29	108548.0
30	124716.5
31	139507.0
32	156140.5
33	171839.5
34	186946.5
35	201707.0
36	218428.0
37	235068.0
38	244480.5
39	251456.0
40	261441.5
41	270647.5
42	277220.0
43	284219.5
44	291898.5
45	301052.5
46	311056.0
47	319417.0
48	329456.0
49	328777.5
50	316923.0
51	307407.5
52	294790.5
53	275841.0
54	255904.0
55	236817.5
56	218570.0
57	199069.0
58	176786.0
59	153724.5
60	127532.0
61	101782.0
62	79780.5
63	61198.0
64	45257.5
65	32885.5
66	24258.0
67	18389.0
68	13924.0
69	10098.0
70	7126.0
71	4991.0
72	3461.5
73	2292.0
74	1476.0
75	940.0
76	599.5
77	375.5
78	233.5
79	149.0
80	97.5
81	61.5
82	45.0
83	35.0
84	24.0
85	18.0
86	15.5
87	11.5
88	8.0
89	3.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018100782552824986
4	0.011212067466174326
5	0.01743665312103069
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.004207116474280093
26-27	0.04299354126269295
28-29	0.08870453835384554
30-31	0.08583310230108122
32-33	0.03605509909140889
34-35	0.003064893420623419
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.326646383872672E-4
56-57	3.096631076522681E-4
58-59	1.4194108550722613E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.5003256831976802E-5
72-73	0.011771637495298155
74-75	0.025358420368762086
76-77	0.005378758474814944
78-79	0.0
80-81	0.0
82-83	0.006036376167975498
84-85	0.043305381677886695
86-87	0.040933057640549865
88-89	0.011020880536587369
90-91	1.8941897376917815E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	386.0
22-23	953.0
24-25	1674.0
26-27	2871.0
28-29	5626.0
30-31	11802.0
32-33	16036.0
34-35	22371.0
36-37	31953.0
38-39	38780.0
40-41	42483.0
42-43	52920.0
44-45	63493.0
46-47	63255.0
48-49	54990.0
50-51	48047.0
52-53	47529.0
54-55	54763.0
56-57	59212.0
58-59	59392.0
60-61	60169.0
62-63	61653.0
64-65	66204.0
66-67	71481.0
68-69	61533.0
70-71	57033.0
72-73	54715.0
74-75	56269.0
76-77	56454.0
78-79	59918.0
80-81	60455.0
82-83	58747.0
84-85	59049.0
86-87	62233.0
88-89	66260.0
90-91	69378.0
92-93	71552.0
94-95	85668.0
96-97	246447.0
98-99	466200.0
100-101	5149199.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.93330566960752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13416658390977	73.09257777239499
2	8.175220588142759	13.559961359058242
3	2.072760779843971	5.157027100043223
4	0.7609854266557763	2.5244414799584076
5	0.32981775202439984	1.3676438221951184
6	0.1777974701697017	0.884719916452008
7	0.10140438370458774	0.588686052500752
8	0.06180585043377304	0.41006107889553156
9	0.03829164353928492	0.2858087320411652
>10	0.14410789254361936	1.8290999553996738
>50	0.003047970527512463	0.17200922937812108
>100	5.12453097716074E-4	0.06643144886051588
>500	5.413698308014013E-5	0.030260959582741092
>1k	2.7068424059691458E-5	0.031271093239459716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.604429144291365E-5	0.0	1.3022145721456825E-5	0.0
3	0.0	2.604429144291365E-5	0.0	1.3022145721456825E-5	0.0
4	0.0	1.041771657716546E-4	0.0	1.3022145721456825E-5	0.0
5	0.0	1.041771657716546E-4	0.0	1.3022145721456825E-5	0.0
6	1.1719931149311142E-4	1.041771657716546E-4	0.0	1.3022145721456825E-5	0.0
7	1.1719931149311142E-4	1.041771657716546E-4	0.0	1.3022145721456825E-5	1.3022145721456825E-5
8	1.1719931149311142E-4	1.1719931149311142E-4	0.0	1.3022145721456825E-5	2.604429144291365E-5
9	1.1719931149311142E-4	1.692878943789387E-4	0.0	2.604429144291365E-5	3.9066437164370474E-5
10-11	1.1719931149311142E-4	1.692878943789387E-4	0.0	3.2555364303642065E-5	3.9066437164370474E-5
12-13	1.1719931149311142E-4	1.8231004010039553E-4	0.0	5.20885828858273E-5	3.9066437164370474E-5
14-15	1.1719931149311142E-4	2.4090969584695122E-4	0.0	5.20885828858273E-5	3.9066437164370474E-5
16-17	1.562657486574819E-4	2.6044291442913647E-4	0.0	5.20885828858273E-5	3.9066437164370474E-5
18-19	1.562657486574819E-4	2.799761330113217E-4	0.0	7.813287432874093E-5	4.557751002509888E-5
20-21	1.562657486574819E-4	2.9950935159350696E-4	0.0	9.115502005019777E-5	9.115502005019777E-5
22-23	1.562657486574819E-4	3.2555364303642057E-4	0.0	1.1719931149311142E-4	9.115502005019777E-5
24-25	1.562657486574819E-4	3.711311530615195E-4	0.0	1.4324360293602507E-4	9.115502005019777E-5
26-27	1.562657486574819E-4	4.0368651736516156E-4	0.0	1.6277682151821029E-4	9.115502005019777E-5
28-29	1.562657486574819E-4	4.0368651736516156E-4	0.0	3.060204244542354E-4	1.041771657716546E-4
30-31	1.562657486574819E-4	4.2973080880807517E-4	0.0	7.162180146801253E-4	1.1719931149311142E-4
32-33	1.562657486574819E-4	4.2973080880807517E-4	0.0	0.0015301021222711769	1.1719931149311142E-4
34-35	1.692878943789387E-4	4.6228617311171725E-4	0.0	0.00250025197851971	1.1719931149311142E-4
36-37	1.692878943789387E-4	5.013526102760877E-4	0.0	0.004238708432334196	1.1719931149311142E-4
38-39	1.8882111296112395E-4	5.208858288582729E-4	0.0	0.007044980835308141	1.6277682151821029E-4
40-41	1.9533218582185236E-4	5.990187031870139E-4	0.0	0.00996194147691447	1.692878943789387E-4
42-43	1.9533218582185236E-4	6.185519217691991E-4	0.0	0.013054701085760467	1.692878943789387E-4
44-45	2.0184325868258078E-4	7.031958689586685E-4	0.0	0.01649254755622507	1.692878943789387E-4
46-47	2.083543315433092E-4	7.683065975659526E-4	0.0	0.02037965805407993	1.692878943789387E-4
48-49	2.083543315433092E-4	7.683065975659526E-4	0.0	0.0245662779035283	1.692878943789387E-4
50-51	2.3439862298622283E-4	8.399283990339651E-4	0.0	0.02856407664001554	1.8231004010039553E-4
52-53	2.3439862298622283E-4	8.529505447554219E-4	0.0	0.032275388170630734	1.8231004010039553E-4
54-55	2.3439862298622283E-4	8.659726904768788E-4	0.0	0.03623412046995361	1.8231004010039553E-4
56-57	2.3439862298622283E-4	8.724837633376072E-4	0.0	0.04043376246512344	1.8882111296112395E-4
58-59	2.3439862298622283E-4	8.789948361983355E-4	0.0	0.04486129201041876	1.9533218582185236E-4
60-61	2.799761330113217E-4	8.85505909059064E-4	0.0	0.04915860009849951	1.9533218582185236E-4
62-63	3.385757887578774E-4	9.050391276412492E-4	0.0	0.05329313136506205	1.9533218582185236E-4
64-65	3.385757887578774E-4	0.0011003713134631016	0.0	0.057440684777346054	1.9533218582185236E-4
66-67	3.5159793447933423E-4	0.0011068823863238301	0.0	0.06224585654856362	2.0184325868258078E-4
68-69	3.5159793447933423E-4	0.0011524598963489289	0.0	0.06692080686256661	2.2137647726476603E-4
70-71	3.5159793447933423E-4	0.0011980374063740276	0.0	0.07143298035505141	2.4090969584695122E-4
72-73	3.776422259222479E-4	0.0012110595520954846	0.0	0.0747080500039978	2.4742076870767964E-4
74-75	3.776422259222479E-4	0.0012436149163991268	0.0	0.0775013002612503	2.4742076870767964E-4
76-77	3.776422259222479E-4	0.0013087256450064109	0.0	0.08077636991019668	2.4742076870767964E-4
78-79	3.776422259222479E-4	0.0013412810093100529	0.0	0.08258644816547918	2.5393184156840805E-4
80-81	3.776422259222479E-4	0.0013412810093100529	0.0	0.08320500008724838	2.734650601505933E-4
82-83	3.776422259222479E-4	0.0013412810093100529	0.0	0.08362170875033499	2.8648720587205013E-4
84-85	3.776422259222479E-4	0.0013412810093100529	0.0	0.08380401879043539	2.8648720587205013E-4
86-87	3.9066437164370473E-4	0.0013412810093100529	0.0	0.08388215166476412	2.8648720587205013E-4
88	3.9066437164370473E-4	0.0013673253007529665	0.0	0.08388866273762485	2.8648720587205013E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12470	0.0	49.74644	1
CTTATAC	6390	0.0	38.10723	1
GTATCAA	22375	0.0	37.1322	1
ATCAACG	26950	0.0	30.303734	3
TCAACGC	27280	0.0	29.968195	4
CAACGCA	27510	0.0	29.651447	5
TATCAAC	27745	0.0	29.636288	2
AACGCAG	28120	0.0	29.078194	6
TATACAC	9445	0.0	28.913282	3
TTATACA	9655	0.0	26.153088	2
CGCAGAG	32200	0.0	25.380444	8
ACGCAGA	32360	0.0	25.215223	7
GCAGAGT	36665	0.0	22.254591	9
GTGGTAT	5630	0.0	22.006418	1
GAGTACT	22650	0.0	21.115452	12-13
GTACATG	24185	0.0	20.899103	1
TACACAT	12865	0.0	20.757544	5
ACATCTC	12090	0.0	20.700981	8
TACATGG	23885	0.0	20.659035	2
TGGTATC	5605	0.0	20.192413	2
>>END_MODULE