FastQCFastQC Report
Fri 27 May 2016
SRR2138631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000037
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131150.050.772851
GTATCAA238400.038.5323831
ATCAACG296500.030.4727653
TCAACGC296900.030.4307614
CAACGCA300850.030.0142565
TATCAAC306550.029.769182
AACGCAG308600.029.3963746
ACGCAGA352300.025.6276027
CGCAGAG357300.025.3051768
GTGGTAT62800.023.1431661
GCAGAGT399650.022.494189
TGGTATC63200.021.2224662
GTACATG259800.020.9328561
TACATGG258950.020.6351642
GAGTACT257700.020.54602812-13
CAGAGTA391100.019.810910-11
ACATGGG264300.019.7279363
AGAGTAC370950.019.35275710-11
GTACTTT280800.018.76391614-15
CATGGGG184250.018.7015764
Produced by FastQC (version 0.11.5)