##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8000037
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80928950703603	33.0	33.0	33.0	33.0	33.0
2	32.807632639699044	33.0	33.0	33.0	33.0	33.0
3	32.80173291698526	33.0	33.0	33.0	33.0	33.0
4	36.754637634800936	37.0	37.0	37.0	37.0	37.0
5	36.76948644112521	37.0	37.0	37.0	37.0	37.0
6	36.77420254431323	37.0	37.0	37.0	37.0	37.0
7	36.75882961541303	37.0	37.0	37.0	37.0	37.0
8	36.755354881483676	37.0	37.0	37.0	37.0	37.0
9	36.75606812818491	37.0	37.0	37.0	37.0	37.0
10-11	36.733668981780966	37.0	37.0	37.0	37.0	37.0
12-13	36.716180062667206	37.0	37.0	37.0	37.0	37.0
14-15	39.00981445460815	40.0	40.0	40.0	37.0	40.0
16-17	38.95419802433413	40.0	40.0	40.0	37.0	40.0
18-19	38.98836911629284	40.0	40.0	40.0	37.0	40.0
20-21	39.00736287826004	40.0	40.0	40.0	37.0	40.0
22-23	39.0446734839911	40.0	40.0	40.0	37.0	40.0
24-25	39.047804016694386	40.0	40.0	40.0	37.0	40.0
26-27	38.96218434357744	40.0	40.0	40.0	37.0	40.0
28-29	38.8922308971938	40.0	40.0	40.0	37.0	40.0
30-31	38.80907591255739	40.0	37.0	40.0	37.0	40.0
32-33	38.77159759249037	40.0	37.0	40.0	37.0	40.0
34-35	38.747037651923	40.0	37.0	40.0	37.0	40.0
36-37	38.708059613924306	40.0	37.0	40.0	37.0	40.0
38-39	38.65846705943931	40.0	37.0	40.0	37.0	40.0
40-41	38.60744057165289	40.0	37.0	40.0	37.0	40.0
42-43	38.532591060035	40.0	37.0	40.0	37.0	40.0
44-45	38.44050403856528	40.0	37.0	40.0	37.0	40.0
46-47	38.35282098694083	40.0	37.0	40.0	37.0	40.0
48-49	38.305418408952804	40.0	37.0	40.0	37.0	40.0
50-51	38.253950208812014	40.0	37.0	40.0	37.0	40.0
52-53	38.18257251918119	40.0	37.0	40.0	37.0	40.0
54-55	38.11609288210893	40.0	37.0	40.0	37.0	40.0
56-57	38.011702228569206	40.0	37.0	40.0	37.0	40.0
58-59	37.88366918088253	40.0	37.0	40.0	33.0	40.0
60-61	37.70924464611168	40.0	37.0	40.0	33.0	40.0
62-63	37.515185617917425	37.0	37.0	40.0	33.0	40.0
64-65	37.27758460624129	37.0	37.0	40.0	33.0	40.0
66-67	37.03335129585031	37.0	37.0	40.0	33.0	40.0
68-69	36.7803176661846	37.0	37.0	40.0	33.0	40.0
70-71	36.504687273704896	37.0	37.0	40.0	33.0	40.0
72-73	36.24778809938087	37.0	37.0	37.0	33.0	40.0
74-75	36.00301146701631	37.0	37.0	37.0	33.0	40.0
76-77	34.36223977485332	35.0	33.0	37.0	30.0	37.0
78-79	35.635443287480804	37.0	35.0	37.0	33.0	37.0
80-81	35.77139285406935	37.0	37.0	37.0	33.0	37.0
82-83	35.70917722394472	37.0	37.0	37.0	33.0	37.0
84-85	35.587853275952256	37.0	37.0	37.0	33.0	37.0
86-87	35.474062989645645	37.0	33.0	37.0	33.0	37.0
88-89	35.39433331449814	37.0	33.0	37.0	33.0	37.0
90-91	35.320890435063646	37.0	33.0	37.0	33.0	37.0
92-93	35.23851784083372	37.0	33.0	37.0	33.0	37.0
94-95	35.1872192118563	37.0	33.0	37.0	33.0	37.0
96-97	35.148616899907935	37.0	33.0	37.0	33.0	37.0
98-99	35.104043000709325	37.0	33.0	37.0	33.0	37.0
100	35.044037208724944	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	4.0
20	33.0
21	115.0
22	440.0
23	1402.0
24	3451.0
25	7247.0
26	13312.0
27	22551.0
28	34414.0
29	49201.0
30	68190.0
31	92247.0
32	125825.0
33	172368.0
34	246272.0
35	384891.0
36	776731.0
37	2401023.0
38	3470852.0
39	129464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07535540148127	19.97947009495081	14.696294529637802	23.24887997393012
2	14.834481390523568	20.64874199956825	41.55192032236851	22.96485628753967
3	18.133231131889737	25.806752020372286	30.6601955752625	25.399821272475474
4	12.600094459516797	17.89695311802664	38.63266724122455	30.87028518123201
5	13.493039844614204	37.60467987685956	35.14434646151261	13.757933817013623
6	30.111048236401906	37.65057586608662	18.448689674810254	13.789686222701219
7	26.642114280221453	33.1764090591081	22.510145890575256	17.67133077009519
8	24.90129733149984	36.06739568829494	20.7234916538511	18.307815326354117
9	25.793968202897062	16.896209355031733	21.1909894916736	36.118832950397604
10-11	24.361356078728136	26.765626208978784	28.3484626383603	20.52455507393278
12-13	25.259739423705163	24.411199598201858	28.593717754055387	21.73534322403759
14-15	22.29724062526211	25.72968725019647	26.74648879748931	25.22658332705211
16-17	21.611806295395883	28.836741630069962	27.422829419413937	22.12862265512022
18-19	21.452932030189363	27.75824661810939	29.62149425058909	21.167327101112157
20-21	22.412989425691272	26.870035616868844	29.637158695632337	21.07981626180755
22-23	22.201714186741384	26.73945300362713	29.449126414568465	21.609706395063018
24-25	21.864548385213205	26.926666822541563	29.569352678657946	21.639432113587283
26-27	21.756607256072556	27.155050672024807	29.43002925437166	21.658312817530977
28-29	21.659589766888075	27.288699192021244	29.473041870585654	21.57866917050503
30-31	21.996933138368053	27.046651353456834	29.364125364439708	21.592290143735404
32-33	21.800700479795722	27.22198747417902	29.243924129850697	21.73338791617456
34-35	21.93504264908322	27.323447881374573	29.231744495219658	21.509764974322547
36-37	22.013468386694697	27.182443037801484	29.117540776325423	21.6865477991784
38-39	21.824896824589707	27.355930881118702	29.154565104263302	21.664607190028292
40-41	22.104832132978515	27.226206009423876	29.046308200916272	21.62265365668134
42-43	22.02755882199495	27.356562224038093	28.73690386227439	21.878975091692567
44-45	22.12612159827839	27.564625558989082	28.448700845226387	21.860551997506136
46-47	22.212453198923836	27.546598045911907	28.04408723845539	22.196861516708868
48-49	22.276098231678347	27.618058113255238	27.954857538396993	22.150986116669426
50-51	22.215348424026274	27.79127350868722	27.80092884171558	22.19244922557092
52-53	22.388270343950595	27.73525870201889	27.640477834971154	22.235993119059362
54-55	22.34318547391434	27.65797585362427	27.7078962039583	22.29094246850309
56-57	22.38633255769723	27.776273353159386	27.600323068634424	22.237071020508957
58-59	22.302823901278437	27.799180705083426	27.689716485839494	22.208278907798643
60-61	22.382617576244556	27.834342078878844	27.612586469886025	22.170453874990574
62-63	22.368470427944004	27.79963230775597	27.533740180351113	22.298157083948915
64-65	22.278024842017864	27.77708693957234	27.55219425202115	22.392693966388645
66-67	22.377685291407214	27.795785489438842	27.408271526947615	22.418257692206332
68-69	22.37088706137043	27.732964665459704	27.523498981995086	22.37264929117478
70-71	22.335640442204514	27.56183599884634	27.540729148205283	22.561794410743865
72-73	22.416030736586677	27.579384099569538	27.545843213781847	22.458741950061935
74-75	22.35809343028347	27.733132647771747	27.43792501580088	22.4708489061439
76-77	22.403737894981916	27.635166306620818	27.438206266569647	22.522889531827616
78-79	22.38770428888027	27.634269257823778	27.40752314799489	22.570503305301063
80-81	22.40806694033708	27.641464037688102	27.417772635731218	22.532696386243607
82-83	22.51365084572494	27.568141024478038	27.524133849584224	22.3940742802128
84-85	22.605943094742916	27.516434779119447	27.431949831431368	22.44567229470627
86-87	22.4204874997993	27.544021548405546	27.524456415780858	22.511034536014293
88-89	22.536321984928833	27.444924407048454	27.44756597786549	22.571187630157223
90-91	22.577416731518998	27.45611168356864	27.46227594303599	22.504195641876372
92-93	22.597601604887224	27.430632902924142	27.51917562354137	22.452589868647266
94-95	22.440882219099475	27.43594030095597	27.572891390184523	22.550286089760036
96-97	22.55933794371755	27.52800447696649	27.63578752669204	22.27687005262392
98-99	23.651065275268717	29.454306429590808	27.771685448774363	19.122942846366108
100	22.532518656640597	30.407654847623117	21.636493752302176	25.423332743434106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1567.0
1	1483.0
2	2246.0
3	3311.0
4	4721.5
5	7844.0
6	10397.0
7	12393.5
8	14028.0
9	13996.5
10	12677.5
11	11781.5
12	13603.0
13	18980.0
14	30247.5
15	44698.5
16	56252.0
17	63027.5
18	63836.0
19	59198.0
20	52071.5
21	44705.0
22	39796.5
23	39470.5
24	43662.0
25	51721.0
26	63203.5
27	76353.5
28	88978.0
29	103272.0
30	119860.5
31	136235.5
32	154267.5
33	171906.5
34	188675.5
35	205997.5
36	225260.0
37	243948.5
38	256122.5
39	263765.5
40	273876.5
41	284652.0
42	293797.0
43	304949.0
44	314696.5
45	324199.0
46	331971.0
47	334914.5
48	334588.0
49	328625.5
50	319028.0
51	309616.0
52	297644.5
53	282038.0
54	266425.0
55	247621.0
56	225522.0
57	200898.0
58	177045.5
59	155161.0
60	129145.5
61	105178.0
62	84447.5
63	64466.5
64	48010.5
65	35670.0
66	26566.0
67	20204.0
68	15120.0
69	10723.0
70	7619.0
71	5377.0
72	3627.0
73	2418.5
74	1571.0
75	993.0
76	652.5
77	421.0
78	278.0
79	181.5
80	124.5
81	84.5
82	58.5
83	41.5
84	28.0
85	16.0
86	10.5
87	7.5
88	6.0
89	4.0
90	2.5
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018749913281651072
4	0.010537451264287904
5	0.017062421086302477
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.004013409037146914
26-27	0.04285134192294412
28-29	0.08838097907207479
30-31	0.08515006423041098
32-33	0.036162166809941845
34-35	0.003199364602418607
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.070963730139898E-4
56-57	2.628430329039714E-4
58-59	2.0362906359613947E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.8436301298820952E-5
72-73	0.012882686400715064
74-75	0.026558522253171825
76-77	0.0048632444008526645
78-79	0.0
80-81	0.0
82-83	0.0056598869716823486
84-85	0.04282602093593813
86-87	0.04013866027056758
88-89	0.010796834164334303
90-91	1.718801966903218E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	425.0
22-23	974.0
24-25	1679.0
26-27	2975.0
28-29	5799.0
30-31	11985.0
32-33	16314.0
34-35	22748.0
36-37	31562.0
38-39	38671.0
40-41	43318.0
42-43	55268.0
44-45	67512.0
46-47	66039.0
48-49	56380.0
50-51	48747.0
52-53	47208.0
54-55	50512.0
56-57	52283.0
58-59	52822.0
60-61	51963.0
62-63	51733.0
64-65	55606.0
66-67	59351.0
68-69	59771.0
70-71	59505.0
72-73	57511.0
74-75	59080.0
76-77	59664.0
78-79	63462.0
80-81	63604.0
82-83	62733.0
84-85	63899.0
86-87	66844.0
88-89	72546.0
90-91	77010.0
92-93	79934.0
94-95	90339.0
96-97	262269.0
98-99	496534.0
100-101	5413354.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.75264979383313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96907683063374	72.79674207652235
2	8.215887844432213	13.597729790714194
3	2.150034632411762	5.337631889417498
4	0.7846710862254586	2.597344464070481
5	0.3402169894633539	1.4076928691486572
6	0.18282490701016574	0.9077547302041414
7	0.11007955139523434	0.6376562196250458
8	0.06156374447828328	0.40756503894467233
9	0.041693515496164145	0.3105223997875044
>10	0.14144008851351397	1.7523479216048996
>50	0.00183347400587859	0.10046958403943357
>100	6.002905683138722E-4	0.08355117406641244
>500	5.1363708249374055E-5	0.03153850848774264
>1k	2.5681657720827204E-5	0.031453333366951226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2499942187767382E-5
2	0.0	0.0	0.0	0.0	1.2499942187767382E-5
3	0.0	0.0	0.0	0.0	1.2499942187767382E-5
4	0.0	0.0	0.0	0.0	1.2499942187767382E-5
5	0.0	0.0	0.0	0.0	1.2499942187767382E-5
6	0.0	0.0	0.0	0.0	1.2499942187767382E-5
7	0.0	0.0	0.0	0.0	1.2499942187767382E-5
8	1.2499942187767382E-5	0.0	0.0	0.0	1.2499942187767382E-5
9	1.2499942187767382E-5	0.0	0.0	2.4999884375534765E-5	3.7499826563302146E-5
10-11	1.2499942187767382E-5	0.0	0.0	2.4999884375534765E-5	3.7499826563302146E-5
12-13	1.2499942187767382E-5	0.0	0.0	3.7499826563302146E-5	3.7499826563302146E-5
14-15	1.2499942187767382E-5	6.249971093883691E-6	0.0	4.999976875106952E-5	4.999976875106953E-5
16-17	1.2499942187767382E-5	1.2499942187767382E-5	0.0	6.249971093883691E-5	6.249971093883691E-5
18-19	1.2499942187767382E-5	1.2499942187767382E-5	0.0	6.249971093883691E-5	8.749959531437168E-5
20-21	1.2499942187767382E-5	1.2499942187767382E-5	0.0	9.374956640825537E-5	8.749959531437168E-5
22-23	1.2499942187767382E-5	1.2499942187767382E-5	0.0	9.999953750213906E-5	1.1249947968990643E-4
24-25	1.2499942187767382E-5	1.2499942187767382E-5	0.0	1.312493929715575E-4	1.312493929715575E-4
26-27	1.2499942187767382E-5	1.2499942187767382E-5	0.0	1.4999930625320858E-4	1.4999930625320858E-4
28-29	1.2499942187767382E-5	1.2499942187767382E-5	0.0	3.2499849688195193E-4	1.7499919062874335E-4
30-31	1.2499942187767382E-5	1.2499942187767382E-5	0.0	8.812459242376004E-4	1.7499919062874335E-4
32-33	1.2499942187767382E-5	1.2499942187767382E-5	0.0	0.0016624923109730618	1.8749913281651073E-4
34-35	1.2499942187767382E-5	1.2499942187767382E-5	0.0	0.002831236905529312	1.8749913281651073E-4
36-37	1.2499942187767382E-5	3.1249855469418453E-5	0.0	0.0045374790141595595	1.8749913281651073E-4
38-39	1.2499942187767382E-5	4.999976875106953E-5	0.0	0.0072874662954683835	1.8749913281651073E-4
40-41	1.2499942187767382E-5	4.999976875106953E-5	0.0	0.010049953518964976	1.8749913281651073E-4
42-43	1.2499942187767382E-5	6.249971093883691E-5	0.0	0.012724941147147194	1.999990750042781E-4
44-45	1.8749913281651073E-5	6.249971093883691E-5	0.0	0.016287424670660897	2.1249901719204548E-4
46-47	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.02003115735589723	2.1249901719204548E-4
48-49	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.023818639838790748	2.1249901719204548E-4
50-51	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.027718621801374167	2.1249901719204548E-4
52-53	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.03163110370614536	2.2499895937981286E-4
54-55	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.036099833038272196	2.3749890156758024E-4
56-57	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.040799811300872735	2.3749890156758024E-4
58-59	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.04498104196268092	2.562488148492313E-4
60-61	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.049087272971362506	2.749987281308824E-4
62-63	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.05346850270817498	2.749987281308824E-4
64-65	2.4999884375534765E-5	6.249971093883691E-5	0.0	0.05752473394810549	2.749987281308824E-4
66-67	2.4999884375534765E-5	8.749959531437168E-5	0.0	0.062005963222420094	2.749987281308824E-4
68-69	2.4999884375534765E-5	8.749959531437168E-5	0.0	0.06676219122486558	2.749987281308824E-4
70-71	2.4999884375534765E-5	8.749959531437168E-5	0.0	0.07103092148198814	2.874986703186498E-4
72-73	3.1249855469418453E-5	9.374956640825537E-5	0.0	0.07438715595940368	3.1249855469418455E-4
74-75	3.7499826563302146E-5	9.999953750213906E-5	0.0	0.07698089396336541	3.1249855469418455E-4
76-77	3.7499826563302146E-5	9.999953750213906E-5	0.0	0.07965588159154763	3.1249855469418455E-4
78-79	3.7499826563302146E-5	9.999953750213906E-5	0.0	0.08149337309314944	3.1249855469418455E-4
80-81	3.7499826563302146E-5	9.999953750213906E-5	0.0	0.08206837043378674	3.374984390697193E-4
82-83	3.7499826563302146E-5	1.0624950859602274E-4	0.0	0.08249961843926472	3.687482945391378E-4
84-85	3.7499826563302146E-5	1.1249947968990643E-4	0.0	0.08267461762989346	3.7499826563302147E-4
86-87	3.7499826563302146E-5	1.1249947968990643E-4	0.0	0.08275586725411395	3.7499826563302147E-4
88	3.7499826563302146E-5	1.1249947968990643E-4	0.0	0.08276211722520783	3.7499826563302147E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13115	0.0	50.77285	1
GTATCAA	23840	0.0	38.532383	1
ATCAACG	29650	0.0	30.472765	3
TCAACGC	29690	0.0	30.430761	4
CAACGCA	30085	0.0	30.014256	5
TATCAAC	30655	0.0	29.76918	2
AACGCAG	30860	0.0	29.396374	6
ACGCAGA	35230	0.0	25.627602	7
CGCAGAG	35730	0.0	25.305176	8
GTGGTAT	6280	0.0	23.143166	1
GCAGAGT	39965	0.0	22.49418	9
TGGTATC	6320	0.0	21.222466	2
GTACATG	25980	0.0	20.932856	1
TACATGG	25895	0.0	20.635164	2
GAGTACT	25770	0.0	20.546028	12-13
CAGAGTA	39110	0.0	19.8109	10-11
ACATGGG	26430	0.0	19.727936	3
AGAGTAC	37095	0.0	19.352757	10-11
GTACTTT	28080	0.0	18.763916	14-15
CATGGGG	18425	0.0	18.701576	4
>>END_MODULE