FastQCFastQC Report
Fri 27 May 2016
SRR2138630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5827892
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115700.049.4391141
GTATCAA207450.037.2230871
ATCAACG241550.031.439263
ACGCAGA253800.029.862237
AACGCAG254600.029.801856
CAACGCA256150.029.5920755
TATCAAC259750.029.5807382
TCAACGC257250.029.518244
CGCAGAG293950.025.7399568
GTGGTAT54900.023.584641
GCAGAGT329050.022.890749
TGGTATC56600.021.5217532
GAGTACT199250.020.86737312-13
CAGAGTA323150.020.24520910-11
GTACTAG19150.020.017131
ACATGGG220800.019.8299713
GTACATG232300.019.74671
TACATGG226500.019.669422
CTCGATA11250.019.48226294
GTACTTT218950.018.93230614-15
Produced by FastQC (version 0.11.5)