##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5827892
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89665886052796	33.0	33.0	33.0	33.0	33.0
2	32.95491320017598	33.0	33.0	33.0	33.0	33.0
3	33.62942141000554	33.0	33.0	33.0	33.0	37.0
4	36.78841714980305	37.0	37.0	37.0	37.0	37.0
5	36.80520949942106	37.0	37.0	37.0	37.0	37.0
6	36.80691457563044	37.0	37.0	37.0	37.0	37.0
7	36.79854602659075	37.0	37.0	37.0	37.0	37.0
8	36.78643238412791	37.0	37.0	37.0	37.0	37.0
9	36.79309293995153	37.0	37.0	37.0	37.0	37.0
10-11	36.788813862713994	37.0	37.0	37.0	37.0	37.0
12-13	37.043533493757266	37.0	37.0	37.0	37.0	38.5
14-15	39.108203703843515	40.0	40.0	40.0	37.0	40.0
16-17	39.06229962737813	40.0	40.0	40.0	37.0	40.0
18-19	39.105699196210224	40.0	40.0	40.0	37.0	40.0
20-21	39.12754464871925	40.0	40.0	40.0	37.0	40.0
22-23	39.15519993453478	40.0	40.0	40.0	37.0	40.0
24-25	39.143492512054195	40.0	40.0	40.0	37.0	40.0
26-27	39.06207929789768	40.0	40.0	40.0	37.0	40.0
28-29	38.99754263182051	40.0	40.0	40.0	37.0	40.0
30-31	38.91754803350408	40.0	40.0	40.0	37.0	40.0
32-33	38.88486911719057	40.0	40.0	40.0	37.0	40.0
34-35	38.864064596278084	40.0	40.0	40.0	37.0	40.0
36-37	38.828324234522356	40.0	37.0	40.0	37.0	40.0
38-39	38.78165385255902	40.0	37.0	40.0	37.0	40.0
40-41	38.73945277933429	40.0	37.0	40.0	37.0	40.0
42-43	38.66729458393179	40.0	37.0	40.0	37.0	40.0
44-45	38.57998585198059	40.0	37.0	40.0	37.0	40.0
46-47	38.49836773919149	40.0	37.0	40.0	37.0	40.0
48-49	38.44957468548159	40.0	37.0	40.0	37.0	40.0
50-51	38.388903996227704	40.0	37.0	40.0	37.0	40.0
52-53	38.31570022916945	40.0	37.0	40.0	37.0	40.0
54-55	38.24307194031633	40.0	37.0	40.0	37.0	40.0
56-57	38.129607389960064	40.0	37.0	40.0	37.0	40.0
58-59	37.998227985333216	40.0	37.0	40.0	37.0	40.0
60-61	37.828772937089234	40.0	37.0	40.0	35.0	40.0
62-63	37.63447848145874	37.0	37.0	40.0	33.0	40.0
64-65	37.40332547868347	37.0	37.0	40.0	33.0	40.0
66-67	37.17397723734932	37.0	37.0	40.0	33.0	40.0
68-69	36.93005186077819	37.0	37.0	40.0	33.0	40.0
70-71	36.68017992823907	37.0	37.0	40.0	33.0	40.0
72-73	36.43274882061465	37.0	37.0	37.0	33.0	40.0
74-75	36.00525417815463	37.0	37.0	37.0	33.0	40.0
76-77	34.81003220339689	37.0	33.0	37.0	30.0	37.0
78-79	35.855200298086515	37.0	37.0	37.0	33.0	37.0
80-81	35.944573986177474	37.0	37.0	37.0	33.0	37.0
82-83	35.87396931702037	37.0	37.0	37.0	33.0	37.0
84-85	35.759778353271585	37.0	37.0	37.0	33.0	37.0
86-87	35.661586999450975	37.0	37.0	37.0	33.0	37.0
88-89	35.59420759903344	37.0	37.0	37.0	33.0	37.0
90-91	35.52981144986917	37.0	35.0	37.0	33.0	37.0
92-93	35.45980170049798	37.0	33.0	37.0	33.0	37.0
94-95	35.428743735416155	37.0	33.0	37.0	33.0	37.0
96-97	35.38889973095707	37.0	33.0	37.0	33.0	37.0
98-99	35.33742121263974	37.0	33.0	37.0	33.0	37.0
100	35.26043542479892	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	0.0
18	0.0
19	6.0
20	8.0
21	68.0
22	247.0
23	774.0
24	2003.0
25	4102.0
26	7923.0
27	13383.0
28	20175.0
29	29402.0
30	40936.0
31	56822.0
32	77305.0
33	107864.0
34	156066.0
35	244509.0
36	506707.0
37	1675710.0
38	2740161.0
39	143719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.24160458869648	0.0	24.721090975355388	29.03730443594813
2	15.325870883662828	23.347120590887368	38.44433062306908	22.882677902380728
3	16.81350100280456	24.85927446644071	32.62939679349639	25.697827737258343
4	13.169928892038946	22.769718150935592	37.428186279346946	26.63216667767852
5	17.478616043626136	36.58313868411465	31.766598039624522	14.17164723263469
6	28.064126788897255	37.15484089272759	20.07195740758408	14.709074910791072
7	26.38439422007134	33.202451246522756	21.915076669231347	18.498077864174558
8	24.470563284288726	31.739246369013014	22.062728684745704	21.727461661952553
9	24.36510491271973	20.601840253731538	23.18205279027134	31.851002043277397
10-11	25.111695618244127	25.9093253615544	27.955777148924515	21.023201871276957
12-13	25.429314750513566	24.180055155449004	28.090422746337783	22.30020734769965
14-15	22.78105874302406	26.4313322896169	26.613353507580445	24.174255459778596
16-17	21.428494213688243	28.609521247133614	27.61732715705782	22.344657382120328
18-19	21.367082643261064	27.627708269130586	29.741397060892687	21.263812026715666
20-21	22.503079879359845	26.868482309064856	29.654800412063366	20.973637399511937
22-23	22.33644709858165	26.50186068273684	29.486804043693333	21.674888174988176
24-25	21.876035774392353	26.894225925191662	29.717986149910185	21.511752150505796
26-27	21.90357463648998	27.12892461184676	29.360426245667686	21.60707450599557
28-29	21.68344461900346	27.079845448221818	29.558569134138	21.67814079863672
30-31	22.087698308991527	26.923692198233567	29.49183349156948	21.496776001205422
32-33	21.87848405115666	27.32070013906475	29.210491710834248	21.59032409894434
34-35	21.928442569247004	27.189422343114778	29.297646035991644	21.58448905164658
36-37	22.05461299996046	27.18514084111352	29.133971263654203	21.626274895271816
38-39	21.703000380456974	27.506960880916964	29.206012876267806	21.58402586235825
40-41	22.186695272948683	27.092193880163666	28.950058959244817	21.77105188764283
42-43	22.088711587456437	27.150512314374257	28.860789842747725	21.899986255421585
44-45	22.10977606089598	27.737338454298854	28.335568969869435	21.81731651493573
46-47	22.28254006629057	27.430429262539928	27.89774789797455	22.389282773194953
48-49	22.23045894731566	27.668991765827442	27.928158040760348	22.17239124609655
50-51	22.249868825603777	27.903531071946546	27.592493053157764	22.254107049291918
52-53	22.45380347920162	27.685449224233317	27.46418093345733	22.396566363107727
54-55	22.415827482315066	27.656004425911718	27.517188531895847	22.41097955987737
56-57	22.544006518673097	27.96156783222765	27.26812582439248	22.22629982470677
58-59	22.46065979718376	27.796233477378994	27.51174313993626	22.231363585500986
60-61	22.46160623795915	27.78404231683452	27.465663604904904	22.288687840301428
62-63	22.41244208884325	27.918267907115148	27.309074364789097	22.36021563925251
64-65	22.40537340243492	27.715564681653408	27.327957856394807	22.551104059516863
66-67	22.516575400219818	27.83104618886427	27.192634346890483	22.459744064025422
68-69	22.461693871294873	27.764727155530146	27.35967799122689	22.4139009819481
70-71	22.526697491920018	27.34941255933921	27.429509752540888	22.694380196199887
72-73	22.565554203149713	27.52753257485887	27.38931585127688	22.517597370714533
74-75	22.35015334332149	27.7240597249776	27.34564895913516	22.58013797256575
76-77	22.618595600699276	27.542578414657815	27.13792050450177	22.700905480141135
78-79	22.439649424194826	27.65619439522416	27.24868548505818	22.655470695522833
80-81	22.429014649442287	27.69264565540427	27.28991671477179	22.58842298038165
82-83	22.74140469235589	27.379966397316835	27.43355007832311	22.44507883200416
84-85	22.83300838484113	27.389828633546387	27.344537940262658	22.43262504134983
86-87	22.42890749972429	27.618828239693823	27.311421256667796	22.640843003914092
88-89	22.66214454179252	27.292895758921244	27.35806939117155	22.686890308114677
90-91	22.785148240582995	27.27977006532315	27.39417194011014	22.540909753983712
92-93	22.62359310038347	27.400119582777094	27.441707295362665	22.53458002147677
94-95	22.651125205492878	27.27040412451508	27.415533646854655	22.662937023137385
96-97	22.584917704784722	27.677435118126574	27.56570304243407	22.171944134654638
98-99	23.704283461673885	29.65147757484831	27.028329327475088	19.615909636002716
100	22.87131534506821	29.932987768435694	21.46700604331449	25.728690843181607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1137.0
1	1093.5
2	1776.5
3	2663.0
4	4074.5
5	7315.0
6	9670.5
7	10829.5
8	11418.0
9	10854.5
10	9633.5
11	8704.5
12	9980.5
13	13873.5
14	22656.5
15	35462.5
16	46552.0
17	53059.5
18	53571.0
19	48935.0
20	42095.5
21	34710.0
22	29433.0
23	28345.5
24	31050.0
25	37220.0
26	46413.5
27	56397.5
28	65299.0
29	76270.0
30	87798.5
31	98327.0
32	111593.0
33	124988.5
34	138930.5
35	153764.5
36	169606.5
37	186640.0
38	194198.5
39	196403.5
40	202537.0
41	205737.5
42	207058.5
43	212298.0
44	219205.5
45	226812.5
46	232577.0
47	238000.5
48	240153.5
49	236675.0
50	232845.0
51	228301.5
52	221428.0
53	212954.5
54	203857.5
55	190284.0
56	173748.5
57	156519.0
58	139216.5
59	121904.5
60	102069.5
61	82076.5
62	64407.0
63	48768.5
64	35863.5
65	26154.5
66	19124.5
67	14300.0
68	10763.0
69	7854.0
70	5694.0
71	4085.0
72	2886.5
73	1937.0
74	1297.5
75	892.5
76	570.0
77	334.0
78	212.0
79	152.0
80	107.5
81	75.5
82	54.5
83	37.0
84	22.5
85	14.0
86	12.5
87	11.5
88	7.0
89	3.5
90	2.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.5
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0295317758119059E-4
2	8.064665577193263E-4
3	0.0040494916515268296
4	0.014361968272576088
5	0.012749035157137436
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.5738939205655153E-5
22-23	2.7456910883018543E-4
24-25	0.008015210707360367
26-27	0.045813069970129225
28-29	0.08615362416801975
30-31	0.07566887198434824
32-33	0.02872157133011716
34-35	0.002453110392818122
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	9.28719910984054E-5
56-57	1.497510529136922E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.9496166420056407E-5
68-69	5.9027823551668724E-5
70-71	0.0013506597824067229
72-73	0.015284906140152266
74-75	0.023710182268991613
76-77	0.003409893612340221
78-79	3.092732809843632E-5
80-81	4.374752097381148E-4
82-83	0.010187334343998286
84-85	0.041465585159148104
86-87	0.03458865025931817
88-89	0.008888993209591594
90-91	5.500616013987406E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	339.0
22-23	778.0
24-25	1412.0
26-27	2591.0
28-29	5518.0
30-31	10039.0
32-33	14017.0
34-35	20277.0
36-37	27930.0
38-39	33772.0
40-41	36464.0
42-43	45773.0
44-45	55498.0
46-47	54744.0
48-49	46234.0
50-51	39773.0
52-53	38769.0
54-55	41088.0
56-57	42746.0
58-59	42196.0
60-61	41786.0
62-63	41529.0
64-65	43717.0
66-67	46687.0
68-69	47673.0
70-71	46814.0
72-73	45400.0
74-75	45278.0
76-77	46584.0
78-79	49475.0
80-81	49824.0
82-83	48305.0
84-85	49012.0
86-87	51003.0
88-89	55367.0
90-91	56971.0
92-93	58750.0
94-95	74837.0
96-97	267883.0
98-99	1030619.0
100-101	3070335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.48338643001881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81251610611653	70.73777796836926
2	8.918885385840277	14.534819688389414
3	2.3847288280425687	5.829473418785955
4	0.9123935821556548	2.9737967532423335
5	0.40786416226598854	1.6617076572437721
6	0.21945439872090083	1.0729132544845552
7	0.1202639567141349	0.6859660120978638
8	0.06738246154475527	0.43924409221257404
9	0.0459542425729434	0.33700565751029726
>10	0.10740791016238506	1.2567144456025754
>50	0.002023487577625085	0.10849167624205358
>100	8.734183036321644E-4	0.1808500109005962
>500	1.800428453917018E-4	0.10154920592079046
>1k	7.201713720874375E-5	0.07969015899797131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7158862930198432E-5	0.0
2	0.0	0.0	0.0	1.7158862930198432E-5	0.0
3	0.0	0.0	0.0	1.0295317758119059E-4	0.0
4	0.0	0.0	0.0	1.0295317758119059E-4	0.0
5	0.0	0.0	0.0	1.3727090344158746E-4	0.0
6	0.0	0.0	0.0	1.3727090344158746E-4	0.0
7	0.0	0.0	0.0	1.3727090344158746E-4	0.0
8	0.0	1.7158862930198432E-5	0.0	1.3727090344158746E-4	0.0
9	0.0	3.4317725860396864E-5	0.0	1.544297663717859E-4	0.0
10-11	0.0	3.4317725860396864E-5	0.0	2.5738294395297645E-4	0.0
12-13	0.0	3.4317725860396864E-5	0.0	3.260183956737702E-4	0.0
14-15	0.0	3.4317725860396864E-5	0.0	3.431772586039686E-4	0.0
16-17	0.0	3.4317725860396864E-5	0.0	3.6033612153416706E-4	0.0
18-19	0.0	3.4317725860396864E-5	0.0	4.0323327885966314E-4	0.0
20-21	0.0	3.4317725860396864E-5	0.0	4.890275935106553E-4	0.0
22-23	0.0	3.4317725860396864E-5	0.0	5.319247508361514E-4	0.0
24-25	0.0	3.4317725860396864E-5	0.0	5.662424766965482E-4	0.0
26-27	0.0	3.4317725860396864E-5	0.0	6.691956542777388E-4	0.0
28-29	0.0	3.4317725860396864E-5	0.0	9.52316892626013E-4	0.0
30-31	0.0	6.863545172079373E-5	0.0	0.0016043536839735533	8.579431465099216E-6
32-33	0.0	6.863545172079373E-5	0.0	0.0028397918149478405	1.7158862930198432E-5
34-35	0.0	8.579431465099215E-5	0.0	0.004804481620455561	1.7158862930198432E-5
36-37	0.0	1.0295317758119059E-4	0.0	0.00799603012547247	1.7158862930198432E-5
38-39	0.0	1.0295317758119059E-4	0.0	0.012543128801975053	1.7158862930198432E-5
40-41	0.0	1.3727090344158746E-4	0.0	0.017330451559500415	1.7158862930198432E-5
42-43	0.0	1.458503349066867E-4	0.0	0.02181749421574731	1.7158862930198432E-5
44-45	0.0	1.544297663717859E-4	0.0	0.0273512275107363	1.7158862930198432E-5
46-47	0.0	1.544297663717859E-4	0.0	0.03407750177937409	1.7158862930198432E-5
48-49	0.0	1.544297663717859E-4	0.0	0.040735140596291075	1.7158862930198432E-5
50-51	0.0	1.544297663717859E-4	0.0	0.04733272339295237	1.7158862930198432E-5
52-53	0.0	1.544297663717859E-4	0.0	0.053595708362474806	1.7158862930198432E-5
54-55	0.0	1.544297663717859E-4	0.0	0.0590350679113477	1.7158862930198432E-5
56-57	0.0	1.7158862930198433E-4	0.0	0.06353926943052479	1.7158862930198432E-5
58-59	0.0	1.7158862930198433E-4	0.0	0.06830943332511996	1.7158862930198432E-5
60-61	0.0	1.7158862930198433E-4	0.0	0.07310533551411041	1.7158862930198432E-5
62-63	0.0	2.0590635516238117E-4	0.0	0.07783260225138008	1.7158862930198432E-5
64-65	0.0	3.088595327435718E-4	0.0	0.08295452283604432	1.7158862930198432E-5
66-67	0.0	3.088595327435718E-4	0.0	0.08770752786770929	1.7158862930198432E-5
68-69	0.0	3.088595327435718E-4	0.0	0.09291524276702451	1.7158862930198432E-5
70-71	0.0	3.088595327435718E-4	0.0	0.09750523860085258	1.7158862930198432E-5
72-73	0.0	3.17438964208671E-4	0.0	0.10121155299377545	1.7158862930198432E-5
74-75	0.0	3.5175669006906786E-4	0.0	0.1038282795906307	1.7158862930198432E-5
76-77	0.0	3.774949844643655E-4	0.0	0.10703698695857783	1.7158862930198432E-5
78-79	0.0	3.774949844643655E-4	0.0	0.10870997609427216	1.7158862930198432E-5
80-81	0.0	3.774949844643655E-4	0.0	0.10937917174854991	1.7158862930198432E-5
82-83	0.0	3.774949844643655E-4	0.0	0.10967087241836328	1.7158862930198432E-5
84-85	0.0	3.9465384739456393E-4	0.0	0.10979956389033976	1.7158862930198432E-5
86-87	0.0	3.9465384739456393E-4	0.0	0.10988535820499076	1.7158862930198432E-5
88	0.0	3.9465384739456393E-4	0.0	0.10988535820499076	1.7158862930198432E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11570	0.0	49.439114	1
GTATCAA	20745	0.0	37.223087	1
ATCAACG	24155	0.0	31.43926	3
ACGCAGA	25380	0.0	29.86223	7
AACGCAG	25460	0.0	29.80185	6
CAACGCA	25615	0.0	29.592075	5
TATCAAC	25975	0.0	29.580738	2
TCAACGC	25725	0.0	29.51824	4
CGCAGAG	29395	0.0	25.739956	8
GTGGTAT	5490	0.0	23.58464	1
GCAGAGT	32905	0.0	22.89074	9
TGGTATC	5660	0.0	21.521753	2
GAGTACT	19925	0.0	20.867373	12-13
CAGAGTA	32315	0.0	20.245209	10-11
GTACTAG	1915	0.0	20.01713	1
ACATGGG	22080	0.0	19.829971	3
GTACATG	23230	0.0	19.7467	1
TACATGG	22650	0.0	19.66942	2
CTCGATA	1125	0.0	19.482262	94
GTACTTT	21895	0.0	18.932306	14-15
>>END_MODULE