FastQCFastQC Report
Fri 27 May 2016
SRR2138629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6971590
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC88550.053.734451
GGTATCA99600.046.2142221
TATACAC122400.041.1708643
TTATACA123100.039.5051542
GTATCAA183350.037.3251381
ATCAACG222200.030.3081423
TCAACGC223850.030.0840934
TATCAAC227050.029.9194582
CAACGCA225800.029.7467615
AACGCAG231650.029.1354186
TACACAT175000.028.816335
ACACATC185000.026.3243716
ACATCTC184250.026.2038638
ATACACA204900.025.8016554
ACGCAGA264700.025.386697
CGCAGAG266650.025.1695778
CACATCT195300.024.6353477
CATCTCC216300.022.3793149
GCAGAGT303400.022.0240769
GTGGTAT51750.020.351281
Produced by FastQC (version 0.11.5)