##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6971590
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.812869374131296	33.0	33.0	33.0	33.0	33.0
2	32.81791183933651	33.0	33.0	33.0	33.0	33.0
3	32.8153244812159	33.0	33.0	33.0	33.0	33.0
4	36.7641490678597	37.0	37.0	37.0	37.0	37.0
5	36.77633294556909	37.0	37.0	37.0	37.0	37.0
6	36.78256122347987	37.0	37.0	37.0	37.0	37.0
7	36.768032543508724	37.0	37.0	37.0	37.0	37.0
8	36.76606484317064	37.0	37.0	37.0	37.0	37.0
9	36.76874199429398	37.0	37.0	37.0	37.0	37.0
10-11	36.7452205307541	37.0	37.0	37.0	37.0	37.0
12-13	36.727921464113635	37.0	37.0	37.0	37.0	37.0
14-15	39.04450541411643	40.0	40.0	40.0	37.0	40.0
16-17	38.98918941016325	40.0	40.0	40.0	37.0	40.0
18-19	39.02259326781982	40.0	40.0	40.0	37.0	40.0
20-21	39.038643783800325	40.0	40.0	40.0	37.0	40.0
22-23	39.070319032518086	40.0	40.0	40.0	37.0	40.0
24-25	39.0773219769774	40.0	40.0	40.0	37.0	40.0
26-27	38.98550379488363	40.0	40.0	40.0	37.0	40.0
28-29	38.90008161272313	40.0	40.0	40.0	37.0	40.0
30-31	38.834080963606276	40.0	38.5	40.0	37.0	40.0
32-33	38.79907473302619	40.0	37.0	40.0	37.0	40.0
34-35	38.77797620639568	40.0	37.0	40.0	37.0	40.0
36-37	38.74450630991467	40.0	37.0	40.0	37.0	40.0
38-39	38.67166095538023	40.0	37.0	40.0	37.0	40.0
40-41	38.658267520106165	40.0	37.0	40.0	37.0	40.0
42-43	38.612723293227305	40.0	37.0	40.0	37.0	40.0
44-45	38.53460661474745	40.0	37.0	40.0	37.0	40.0
46-47	38.45978549345274	40.0	37.0	40.0	37.0	40.0
48-49	38.40938375384369	40.0	37.0	40.0	37.0	40.0
50-51	38.31622618875406	40.0	37.0	40.0	37.0	40.0
52-53	38.248301405917545	40.0	37.0	40.0	37.0	40.0
54-55	38.18261002019999	40.0	37.0	40.0	37.0	40.0
56-57	38.07110041144378	40.0	37.0	40.0	37.0	40.0
58-59	37.958556416105964	40.0	37.0	40.0	35.0	40.0
60-61	37.771114058960336	40.0	37.0	40.0	33.0	40.0
62-63	37.58778500083324	38.5	37.0	40.0	33.0	40.0
64-65	37.380430235250415	37.0	37.0	40.0	33.0	40.0
66-67	37.15888326589325	37.0	37.0	40.0	33.0	40.0
68-69	36.93040878095027	37.0	37.0	40.0	33.0	40.0
70-71	36.67715691496848	37.0	37.0	40.0	33.0	40.0
72-73	36.43572953128141	37.0	37.0	37.0	33.0	40.0
74-75	36.19795643918218	37.0	37.0	37.0	33.0	40.0
76-77	34.576023456661176	35.0	33.0	37.0	30.0	37.0
78-79	35.8081310280101	37.0	37.0	37.0	33.0	37.0
80-81	35.918421399100524	37.0	37.0	37.0	33.0	37.0
82-83	35.84924915142429	37.0	37.0	37.0	33.0	37.0
84-85	35.72876284974527	37.0	37.0	37.0	33.0	37.0
86-87	35.61964742805311	37.0	37.0	37.0	33.0	37.0
88-89	35.54439413471794	37.0	37.0	37.0	33.0	37.0
90-91	35.47916798773553	37.0	33.0	37.0	33.0	37.0
92-93	35.40825484147952	37.0	33.0	37.0	33.0	37.0
94-95	35.36383118428644	37.0	33.0	37.0	33.0	37.0
96-97	35.33265353529757	37.0	33.0	37.0	33.0	37.0
98-99	35.28880541132784	37.0	33.0	37.0	33.0	37.0
100	35.22904850112081	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	23.0
21	106.0
22	425.0
23	1261.0
24	3131.0
25	6441.0
26	11563.0
27	18900.0
28	28184.0
29	40120.0
30	54007.0
31	73353.0
32	99076.0
33	135264.0
34	197135.0
35	306852.0
36	618594.0
37	1989280.0
38	3239037.0
39	148836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.513025005773436	20.545413600053934	14.784001927824212	24.15755946634842
2	14.233195010033578	20.59855786126264	42.18740631620621	22.98084081249758
3	17.582407809443566	26.207448457331083	31.229798878484083	24.98034485474127
4	12.195212922383927	18.599783383661965	38.08843971682076	31.11656397713335
5	13.194314229504473	37.49075543666404	35.54391316430123	13.771017169530264
6	29.441892021762612	37.779114950821835	18.68853446631256	14.090458561102992
7	25.944339813442845	33.04279224681888	22.43172647846474	18.58114146127354
8	24.473111585735822	36.21449339390297	21.35683538475441	17.9555596356068
9	25.057081670035096	16.881141891591444	20.982903469653262	37.07887296872019
10-11	23.864269126555058	26.674653845105635	28.64493609061921	20.81614093772009
12-13	24.936542453012873	24.209993703014664	28.595248716576847	22.258215127395616
14-15	22.222670868481938	25.740024872374885	26.166627698989757	25.87067656015342
16-17	21.75627797962875	29.171049932655247	26.83306103772597	22.23961104999003
18-19	21.71512524402611	27.695382258566553	28.708687688174432	21.880804809232902
20-21	22.456846665366605	26.755651999724307	28.90237946963246	21.885121865276634
22-23	21.965345946283644	27.391457998326047	28.595063456736515	22.048132598653794
24-25	22.015166239356034	27.55743225647419	28.8491136957159	21.57828780845388
26-27	22.077941283625194	27.696201931895146	28.74298314159275	21.48287364288691
28-29	21.336842857181868	27.191637853013066	29.171655787257716	22.299863502547343
30-31	22.068670078263118	26.887986028540347	29.40912668374504	21.634217209451503
32-33	21.701405940745428	27.65951631692302	28.943238969831576	21.69583877249998
34-35	21.555264782211385	27.72524479658738	29.491870084836002	21.227620336365234
36-37	22.167144065334853	27.25676887307872	28.69358709192879	21.882499969657633
38-39	21.812917904224033	27.082110407186853	29.02065928277759	22.08431240581152
40-41	21.857500644236787	26.964424791425344	29.182780569450053	21.995293994887817
42-43	22.08840739978804	26.911459660104082	29.17935848599784	21.82077445411004
44-45	21.782976392940938	27.413681875789763	28.75169386280939	22.05164786845991
46-47	22.4361611922791	26.93694205689059	27.97178278854671	22.655113962283597
48-49	22.162021443870543	27.06633815682895	28.238529801107543	22.533110598192962
50-51	21.961370600827276	27.476252646447037	28.241486341660988	22.320890411064706
52-53	21.973776629460566	27.78137794950623	28.370998864661058	21.873846556372147
54-55	21.95715201415788	27.78486106532697	27.881610578575128	22.37637634194002
56-57	22.412856460927255	28.151167159526995	27.3249657707947	22.11101060875105
58-59	21.80208814597211	28.588301462769046	28.04456215300297	21.565048238255876
60-61	22.377570372378788	28.855849129721463	27.106311061608636	21.66026943629111
62-63	21.981365667928966	29.121176610870574	27.04493702895065	21.85252069224981
64-65	22.007990430406814	28.805361690743624	27.2062178368435	21.980430042006063
66-67	22.199839938616957	28.426450679787973	27.24121615236722	22.132493229227848
68-69	22.259739483629946	27.98098486150249	27.52935648650076	22.229919168366802
70-71	22.270911584609323	27.72662255927831	27.560709377486404	22.44175647862596
72-73	22.381471254960104	27.736640263727224	27.485413670649596	22.396474810663076
74-75	22.255833558394816	27.935324733751678	27.449932340524253	22.358909367329254
76-77	22.3679388639421	27.865699345595978	27.378578755298154	22.387783035163764
78-79	22.28298132541263	27.86708520920243	27.355576785974034	22.494356679410902
80-81	22.31006738308613	27.870826868722226	27.41588449028336	22.40322125790829
82-83	22.373186082891568	27.835792188570448	27.54448470283083	22.24653702570716
84-85	22.512944075248136	27.822413477103865	27.381394819113236	22.28324762853476
86-87	22.270696898043347	27.818730234706624	27.497130846394008	22.41344202085602
88-89	22.376937024583704	27.789417371557107	27.429327470880665	22.404318132978524
90-91	22.387895115672986	27.87979018246327	27.419318958955046	22.31299574290869
92-93	22.44482148246007	27.84043641270181	27.459902573754576	22.254839531083547
94-95	22.272855590919683	27.91232187758665	27.488920751164557	22.325901780329108
96-97	22.38768388802579	28.002085647345194	27.511408933038258	22.09882153159077
98-99	23.49807234885119	29.897202346147772	27.675340675156463	18.929384629844577
100	22.353333932538103	30.935252770927846	21.557202542700658	25.15421075383339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1759.0
1	1566.5
2	2068.0
3	2937.0
4	4118.0
5	6809.5
6	8932.5
7	10181.0
8	10776.0
9	10432.0
10	9631.0
11	8950.0
12	10135.0
13	13870.0
14	22080.0
15	32704.0
16	41128.0
17	46327.0
18	47247.5
19	44366.5
20	40031.5
21	35801.5
22	33585.0
23	35092.5
24	40709.5
25	49564.0
26	61294.5
27	74963.0
28	88413.0
29	103066.0
30	117062.5
31	130566.5
32	146704.5
33	162140.0
34	176899.5
35	191626.0
36	206700.5
37	220797.0
38	229090.5
39	235151.0
40	244943.0
41	253818.5
42	259393.5
43	265674.0
44	274513.0
45	291388.0
46	315563.0
47	332989.0
48	327555.0
49	316292.5
50	307124.0
51	287554.5
52	266648.0
53	247604.5
54	231889.0
55	216520.5
56	199390.5
57	180269.0
58	160470.5
59	141483.5
60	118668.5
61	95227.0
62	75047.5
63	57938.5
64	43042.5
65	31365.5
66	22998.5
67	17228.0
68	12834.0
69	9175.5
70	6807.5
71	5037.5
72	3442.5
73	2249.0
74	1481.5
75	938.0
76	602.5
77	387.5
78	258.5
79	186.5
80	130.0
81	90.5
82	67.0
83	45.0
84	27.5
85	17.5
86	13.5
87	12.0
88	9.0
89	7.0
90	5.5
91	4.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001907742710056099
4	0.010614508311590326
5	0.01831719880256871
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.003938401111016497
26-27	0.04225826928097221
28-29	0.08755357756238892
30-31	0.08482091692295568
32-33	0.03556818407912141
34-35	0.002849944422467081
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	8.639744489767322E-5
56-57	2.699582088812757E-4
58-59	8.033616507603737E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.460849486271502E-5
72-73	0.012460655502082337
74-75	0.02561695036682821
76-77	0.004811824315580251
78-79	0.0
80-81	0.0
82-83	0.005943762845144646
84-85	0.04330779440422236
86-87	0.04120388196428274
88-89	0.010875726322920706
90-91	1.8140031880628662E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	71.0
20-21	395.0
22-23	920.0
24-25	1708.0
26-27	3308.0
28-29	7218.0
30-31	16993.0
32-33	21931.0
34-35	29393.0
36-37	42582.0
38-39	49521.0
40-41	51349.0
42-43	59883.0
44-45	70096.0
46-47	68563.0
48-49	60234.0
50-51	53530.0
52-53	52924.0
54-55	65128.0
56-57	73875.0
58-59	71477.0
60-61	73444.0
62-63	74609.0
64-65	78544.0
66-67	86687.0
68-69	64498.0
70-71	53994.0
72-73	51699.0
74-75	52527.0
76-77	52381.0
78-79	54378.0
80-81	54994.0
82-83	52715.0
84-85	53482.0
86-87	55905.0
88-89	58445.0
90-91	60932.0
92-93	62439.0
94-95	75165.0
96-97	212454.0
98-99	401982.0
100-101	4439217.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.51411125476511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.8692507728947	75.10705746948044
2	7.808826082751523	13.199119926535477
3	1.8912569331072195	4.795136965679082
4	0.6993376935001314	2.364156145324837
5	0.32326201008850447	1.3660100742529433
6	0.14664862969462086	0.7436327163222022
7	0.08354034381746574	0.49422365381554395
8	0.052182020055886305	0.35280936388012474
9	0.032358334773529	0.2461262314562068
>10	0.09153463057049373	1.1216703697409633
>50	0.0011993177898566975	0.06700746189246624
>100	5.503749769660095E-4	0.09668137179087143
>500	2.6427989567940113E-5	0.019029744190981322
>1k	2.6427989469993553E-5	0.027338505637835187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8687860301595477E-5	4.303179045239321E-5	0.0	0.0	0.0
3	2.8687860301595477E-5	4.303179045239321E-5	0.0	0.0	0.0
4	2.8687860301595477E-5	1.864710919603706E-4	0.0	0.0	0.0
5	2.8687860301595477E-5	1.864710919603706E-4	0.0	1.4343930150797738E-5	0.0
6	2.8687860301595477E-5	2.0081502211116833E-4	0.0	4.303179045239321E-5	0.0
7	2.8687860301595477E-5	2.0081502211116833E-4	0.0	5.7375720603190954E-5	0.0
8	2.8687860301595477E-5	2.2950288241276382E-4	0.0	5.7375720603190954E-5	0.0
9	2.8687860301595477E-5	3.872861140715389E-4	0.0	5.7375720603190954E-5	0.0
10-11	2.8687860301595477E-5	3.872861140715389E-4	0.0	7.889161582938755E-5	0.0
12-13	3.585982537699434E-5	4.0163004422233666E-4	0.0	8.606358090478642E-5	0.0
14-15	4.303179045239321E-5	5.092095203533197E-4	0.0	8.606358090478642E-5	0.0
16-17	4.303179045239321E-5	5.163814854287186E-4	0.0	1.0757947613098304E-4	0.0
18-19	4.303179045239321E-5	5.52241310805713E-4	0.0	1.1475144120638191E-4	1.4343930150797738E-5
20-21	4.303179045239321E-5	5.881011361827072E-4	0.0	1.1475144120638191E-4	2.8687860301595477E-5
22-23	4.303179045239321E-5	6.741647170874937E-4	0.0	1.1475144120638191E-4	2.8687860301595477E-5
24-25	4.303179045239321E-5	7.10024542464488E-4	0.0	1.5061126658337624E-4	2.8687860301595477E-5
26-27	5.7375720603190954E-5	7.315404376906846E-4	0.0	1.864710919603706E-4	2.8687860301595477E-5
28-29	5.7375720603190954E-5	7.315404376906846E-4	0.0	4.446618346747299E-4	2.8687860301595477E-5
30-31	5.7375720603190954E-5	8.319479487462688E-4	0.0	0.0012120620977424089	2.8687860301595477E-5
32-33	5.7375720603190954E-5	8.319479487462688E-4	0.0	0.0027612065540285648	2.8687860301595477E-5
34-35	5.7375720603190954E-5	9.395274248772519E-4	0.0	0.004898452146497428	2.8687860301595477E-5
36-37	5.7375720603190954E-5	0.001047106901008235	0.0	0.007946537303541947	2.8687860301595477E-5
38-39	7.171965075398868E-5	0.0010614508311590327	0.0	0.012199512593253477	2.8687860301595477E-5
40-41	7.171965075398868E-5	0.0011188265517622234	0.0	0.016710678625679366	2.8687860301595477E-5
42-43	7.171965075398868E-5	0.0011403424469884202	0.0	0.02069829120760114	2.8687860301595477E-5
44-45	7.889161582938755E-5	0.0012694378183455996	0.0	0.025438960122439788	2.8687860301595477E-5
46-47	8.606358090478642E-5	0.0013913612246273805	0.0	0.030387616024465006	2.8687860301595477E-5
48-49	8.606358090478642E-5	0.0013985331897027795	0.0	0.03509242511392666	2.8687860301595477E-5
50-51	8.606358090478642E-5	0.0015491444562861557	0.0	0.04052877464107901	2.8687860301595477E-5
52-53	8.606358090478642E-5	0.0015634883864369534	0.0	0.045936436307929755	2.8687860301595477E-5
54-55	9.32355459801853E-5	0.0015706603515123524	0.0	0.05097115579085976	2.8687860301595477E-5
56-57	1.1475144120638191E-4	0.0015778323165877512	0.0	0.05599153134363897	5.020375552779208E-5
58-59	1.2909537135717965E-4	0.0015778323165877512	0.0	0.06106211065194597	7.171965075398868E-5
60-61	1.2909537135717965E-4	0.0016065201768893467	0.0	0.06610400209995138	7.171965075398868E-5
62-63	1.2909537135717965E-4	0.0016638958974925376	0.0	0.07149014787157593	7.171965075398868E-5
64-65	1.2909537135717965E-4	0.0018216791291513128	0.0	0.07642445984345034	7.171965075398868E-5
66-67	1.362673364325785E-4	0.0018360230593021105	0.0	0.08147352325653115	7.171965075398868E-5
68-69	1.577832316587751E-4	0.001864710919603706	0.0	0.08675208955202472	7.171965075398868E-5
70-71	1.577832316587751E-4	0.0018790548497545035	0.0	0.09101940877188705	7.171965075398868E-5
72-73	1.577832316587751E-4	0.0018790548497545035	0.0	0.09456235951913408	7.171965075398868E-5
74-75	1.577832316587751E-4	0.0019149146751314978	0.0	0.09814834205683352	7.889161582938755E-5
76-77	1.577832316587751E-4	0.0020081502211116834	0.0	0.10129683472493362	8.606358090478642E-5
78-79	1.577832316587751E-4	0.002036838081413279	0.0	0.1033265008412715	8.606358090478642E-5
80-81	1.7212716180957285E-4	0.002036838081413279	0.0	0.10400066555835899	8.606358090478642E-5
82-83	1.864710919603706E-4	0.0020440100464886777	0.0	0.10438795167243053	8.606358090478642E-5
84-85	1.864710919603706E-4	0.002079869871865672	0.0	0.10456007883424011	8.606358090478642E-5
86-87	2.0081502211116833E-4	0.00209421380201647	0.0	0.1046533143802203	8.606358090478642E-5
88	2.0081502211116833E-4	0.0021659334527704584	0.0	0.10466765831037109	8.606358090478642E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8855	0.0	53.73445	1
GGTATCA	9960	0.0	46.214222	1
TATACAC	12240	0.0	41.170864	3
TTATACA	12310	0.0	39.505154	2
GTATCAA	18335	0.0	37.325138	1
ATCAACG	22220	0.0	30.308142	3
TCAACGC	22385	0.0	30.084093	4
TATCAAC	22705	0.0	29.919458	2
CAACGCA	22580	0.0	29.746761	5
AACGCAG	23165	0.0	29.135418	6
TACACAT	17500	0.0	28.81633	5
ACACATC	18500	0.0	26.324371	6
ACATCTC	18425	0.0	26.203863	8
ATACACA	20490	0.0	25.801655	4
ACGCAGA	26470	0.0	25.38669	7
CGCAGAG	26665	0.0	25.169577	8
CACATCT	19530	0.0	24.635347	7
CATCTCC	21630	0.0	22.379314	9
GCAGAGT	30340	0.0	22.024076	9
GTGGTAT	5175	0.0	20.35128	1
>>END_MODULE